OBSERVATIONS ON THE QUASISPECIES COMPOSITION OF THREE ANIMAL PATHOGENIC RNA VIRUSES

I. Kiss; S. Kecskeméti; J. Tanyi; S. Belák; C. Ros; S. B. Klingeborn

doi:10.1556/avet.47.1999.4.7

Jump to Content

Acta Veterinaria Hungarica

Volume/Issue:: Volume 47: Issue 4

OBSERVATIONS ON THE QUASISPECIES COMPOSITION OF THREE ANIMAL PATHOGENIC RNA VIRUSES

Authors:

I. Kiss

I. Kiss Veterinary Institute of Debrecen H-4002 Debrecen, P.O. Box 51, Hungary

Search for other papers by I. Kiss in
Current site
Google Scholar
PubMed

S. Kecskeméti

S. Kecskeméti Veterinary Institute of Debrecen H-4002 Debrecen, P.O. Box 51, Hungary

Search for other papers by S. Kecskeméti in
Current site
Google Scholar
PubMed

J. Tanyi

J. Tanyi Veterinary Institute of Debrecen Please ask the editor of the journal.

Search for other papers by J. Tanyi in
Current site
Google Scholar
PubMed

S. Belák

S. Belák Veterinary Institute of Debrecen Please ask the editor of the journal.

Search for other papers by S. Belák in
Current site
Google Scholar
PubMed

C. Ros

C. Ros Veterinary Institute of Debrecen Please ask the editor of the journal.

Search for other papers by C. Ros in
Current site
Google Scholar
PubMed

, and

S. B. Klingeborn

S. B. Klingeborn Veterinary Institute of Debrecen Please ask the editor of the journal.

Search for other papers by S. B. Klingeborn in
Current site
Google Scholar
PubMed

View More View Less

Pages:: 471–480

Online Publication Date:: 22 Jul 2005

Publication Date:: 01 Nov 1999

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/avet.47.1999.4.7

Keywords:: csfv; csfv; csfv; fcov; rhdv; rt-pcr; sscp; quasispecies

Restricted access

Purchase article

USD $30.00

1 year subscription (Individual Only)

USD $836.00

USD $30.00

Click here to view the full Terms and Conditions

USD $836.00

Click here to view the full Terms and Conditions

The quasispecies nature of three animal pathogenic RNA viruses of field origin was examined by testing variants of classical swine fever virus (CSFV) originating from geographically different areas, feline coronavirus (FCoV) detected from the same animal by successive sampling, and rabbit haemorrhagic disease virus (RHDV) originating from successive outbreaks in the same geographic area. Clinical samples were investigated using reverse transcriptase polymerase chain reaction (RT-PCR) and ensuing single strand conformational polymorphism (SSCP) assay. By the combination of these methods even subtle differences could be detected among the amplified fragments of the same virus species of different origin. FCoV proved to comprise the most and CSFV the less heterogeneous virus quasispecies. The results show that the combination of RT-PCR and SSCP provides novel and highly sensitive means for the characterisation of RNA viruses, with special regard to genome composition, evolution, features of pathogenicity and molecular epizootiology.

Save
Cite
Email this content

Share Link

Copy this link, or click below to email it to a friend
Email this content
or copy the link directly:

https://akjournals.com/abstract/journals/004/47/4/article-p471.xml
The link was not copied. Your current browser may not support copying via this button.

Link copied successfully

Collapse
Expand

Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA

University of Veterinary Medicine,

H-1078 Budapest, István utca 2., Hungary

Phone: (36 20) 560 4183 (ed.-in-chief) or (36 1) 478 4100/8430 (editor)

E-mail: acta.veterinaria@univet.hu (ed.-in-chief)

Indexing and Abstracting Services:

Biological Abstracts
BIOSIS Previews
CAB Abstracts
Chemical Abstracts
Current Contents: Agriculture, Biology and Environmental Sciences
Elsevier Science Navigator
Focus On: Veterinary Science and Medicine
Global Health
Index Medicus
Index Veterinarius
Medline
Science Citation Index
Science Citation Index Expanded (SciSearch)
SCOPUS
The ISI Alerting Services
Zoological Abstracts

2023
Web of Science
Journal Impact Factor	0.7
Rank by Impact Factor	Q3 (Veterinary Sciences)
Journal Citation Indicator	0.4
Scopus
CiteScore	1.8
CiteScore rank	Q2 (General Veterinary)
SNIP	0.39
Scimago
SJR index	0.258
SJR Q rank	Q3

Acta Veterinaria Hungarica
Publication Model	Hybrid
Submission Fee	none
Article Processing Charge	1100 EUR/article
Printed Color Illustrations	40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency	World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100%
Further Discounts	Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2025	Online subsscription: 832 EUR / 916 USD Print + online subscription: 960 EUR / 1054 USD
Subscription Information	Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title	Individual articles are sold on the displayed price.

Acta Veterinaria Hungarica
Language	English
Size	A4
Year of Foundation	1951
Volumes per Year	1
Issues per Year	4
Founder	Magyar Tudományos Akadémia
Founder's Address	H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher	Akadémiai Kiadó
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-6290 (Print)
ISSN	1588-2705 (Online)