The pathogenesis of chicken infectious anaemia virus (CAV) infection was studied in 6-week-old and one-day-old SPF chickens inoculated intramuscularly with graded doses of Cux-1 strain (106−102 TCID50/chicken). Viraemia, virus shedding, development of virus neutralizing (VN) antibodies and CAV distribution in the thymus were studied by virus isolation, polymerase chain reaction (PCR), immunocytochemistry (IP) and in situ hybridization until postinfection day (PID) 28. In 6-week-old chickens infected with high doses of CAV, viraemia and VN antibodies could be detected 4 PID and onward without virus shedding or contact transmission to sentinel birds. However, virus shedding and contact transmission were demonstrated in one-day-old infected chickens. In the 6-weekold groups infected with lower doses, VN antibodies developed by PID 14, transient viraemia and virus shedding were detected. The thymus cortex of all 1-dayold inoculated chickens stained with VP3-specific mAb. Cells with positive in situ hybridization signal were fewer and scattered throughout the thymus tissue of the one-day-old inoculated chickens as compared to IP-positive cells. These results suggest that early immune response induced by high doses of CAV in 6-week-old chickens curtails viral replication and prevents virus shedding.
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-
Adair, B. M. (2000): Immunopathogenesis of chicken anemia virus infection. Dev. Comp. Immunol. 24, 247–255.
-
Akiyama, Y. and Kato, S. (1974): Two cell lines from lymphomas of Marek’s disease. Biken J. 17, 105–117.
-
Allan, G. M., Smyth, J. A., Todd, D. and McNulty, M. S. (1993): In situ hybridization for the detection of chicken anaemia virus in formalin-fixed, paraffin-embedded sections. Avian Dis. 37, 177–182.
-
Boom, R., Sol, C. J. A., Salimans, M. M. M., Jansen, C. L., Wertheim-van Dillen, P. M. E. and van derNoordaa, J. (1990): Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28, 495–503.
-
Bülow, V. v. and Schat, K. A. (1997): Chicken infectious anemia. In: Calnek, B. W. (ed.) Diseases of Poultry. 9th edition. Iowa State University Press, Ames, Iowa, pp. 739–756.
-
Bülow, V. v., Fuchs, B. and Bertram, M. (1985): Untersuchungen über den Erreger der infektiosen Anämie bei Hühnerküken (CAA) in vitro: Vermehrung, Titration, Serumneutralizationstest und indirekt Immunfluoreszenztest. Zbl. Vet-med. B 32, 679–693.
-
Drén, Cs. N., Koch, G., Kant, A., Verschueren, C. A. J., van der Eb, A. J. and Noteborn, M. H. M. (1994): A hot start PCR for the laboratory diagnosis of CAV. In: Kaleta, E. F. (ed.) Proceedings of the International Symposium on Infectious Bursal Disease and Chicken Infectious Anaemia. Rauischholzhausen, Germany, pp. 413–420.
-
Farkas, T., Maeda, K., Sugiura, H., Kai, K., Hirai, K., Otsuki, K. and Hayashi, T. (1998): A serological survey of chickens, Japanese quail, pigeon, ducks and crows for antibodies to chicken anaemia virus (CAV) in Japan. Avian Pathol. 27, 316–320.
-
Koch, G., van Roozelaar, D. J., Verschueren, C. A. J., van der Eb, A. J. and Noteborn, M. H. M. (1995): Immunogenic and protective properties of chicken anaemia virus proteins expressed by baculovirus. Vaccine 13, 763–770.
-
McNulty, M. S., Connor, T. J. and McNeilly, F. (1990): Influence of virus dose on experimental anaemia due to chicken anaemia agent. Avian Pathol. 19, 167–171.
-
Noteborn, M. H. M. and Koch, G. (1995): Chicken anaemia virus infection: molecular basis of pathogenicity. Avian Pathol. 24, 11–31.
-
Noteborn, M. H. M., de Boer, G. F., van Roozelaar, D. J., Karreman, C., Kranenburg, O., Vos, J. G., Jeurissen, S. H. M., Hoeben, R. C., Zantema, A., Koch, G., van Ormondt, H. and van der Eb, A. J. (1991): Characterization of cloned chicken anemia virus DNA that contain all elements for the infectious replication cycle. J. Virol. 65, 3131–3139.
-
Vielitz, E., von Bülow, V., Landgraf, H. and Conrad, C. (1987): Anemia in broilers-development of a vaccine for breeder stock. Zbl. Vet.-Med.? 34, 553–557.
-
Yuasa, N., Taniguchi, T. and Yoshida, I. (1979): Isolation and some characteristics of an agent inducing anemia in chicks. Avian Dis. 23, 366–385.
-
Yuasa, N., Taniguchi, M., Goda, M., Shibatani, M., Imada, T. and Hihara, H. (1983): Isolation of chicken anemia agent with MDCC-MSB1 cells from chickens in the field. Natl. Inst. Anim. Health Q. 23, 75–77.
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