This paper describes the production of an enzyme preparation from the fungus Thermomyces lanuginosus. Thermal resistance, pH stability and lignocellulolytic activity of the enzyme preparation high in xylanase were studied on a variety of grains and forages. The enzyme preparation preserved more than 70% of its original xylanase activity for 4 and 1 h at 60 and 70 °C, respectively. The xylanase activity remained over 80% when the preparation was incubated for 30 min at pH 4.5. In vitro digestibility studies indicated that the enzyme digested 7.5, 8.5 and 8.0% of the dry matter (DM) of barley meal, wheat bran and oat meal samples, respectively. When applying 60-min incubation, 7.5, 7.3 and 8.4% of DM of the oat straw, alfalfa hay and triticale straw was digested, respectively. When the time of digestion was increased to 360 min, the sunflower hull showed 15.8% DM digestibility.
Alam, M., Gomes, I., Mohuiddin, G. and Hoq, M. M. (1994): Production and characterization of thermostable xylanase by Thermomyces lanuginosus and Thermoascus auranticus grown on lignocelluloses. Enzyme Microb. Technol. 16, 298–302.
Bailey, M., Biely, P. and Poutanen, K. (1992): Inter laboratory testing of methods for assay xylanase activity. J. Biotechnol. 23, 257–270.
Bakalova, N., Petrova, S. and Kolev, D. (1996): Separation of enzymes from polyenzyme mixtures used in medicine and pharmacy. Pharmazie 51, 761–764.
Bedford, M. R. and Morgan, A. J. (1997): Method and composition for treatment and prophylaxis of coccidiosis. US Patent 5-624-678.
Bennet, N. I., Ryen, J., Biely, P. and Vrasanska, M. (1998): Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Thermomyces lanuginosus ATCC 46882. Carbohydr. Res. 306, 445–455.
Caesar, T. and Mrsa, V. (1996): Purification and properties of the xylanase produced by Thermomyces lanuginosus. Enzyme Microb. Technol. 19, 289–296.
Gomes, J., Gomes, I., Kreiner, W., Esterbauer, H., Sinner, M. and Steiner, W. (1993 a): Production of high level of cellulase-free and thermostable xylanase by a wild strain of Thermomyces lanuginosus using beechwood xylane. J. Biotechnol. 30, 283–297.
Gomes, J., Purkarthofer, H., Hayn, M., Kapplmüller, J., Sinner, M. and Steiner, W. (1993 b): Production of a high level of cellulase-free xylanase by the thermophilic fungus Thermomyces lanuginosus in laboratory and pilot scales using lignocellulosic materials. Appl. Microbiol. Biotechnol. 39, 700–707.
Hoq, M. M. and Deckwer, W.-D. (1995): Cellulase-free xylanase by thermophilic fungi: a comparison of xylanase production by two Thermomyces lanuginosus strains. Appl. Microbiol. Biotechnol. 43, 604–609.
Hoq, M. M., Hempel, C. and Deckwer, W.-D. (1994): Cellulase-free xylanase by Thermomyces lanuginosus RT9: Effect of agitation, aeration, and medium components on production. J. Biotechnol. 37, 49–58.
Lisching, T., Purkarthofer, H. and Steiner, W. (1993): thermostability of endo-beta-xylanase from the thermophilic fungus Thermomyces lanuginosus. Biotech. Letter 15, 411–414.
Miller, G. L. (1959): Use of 2,4-dinitrosalicylic acid reagent for the determination of reducing sugar. Anal. Chem. 31, 426–428.
Pack, M. and Bedford, M. (1999): Best-cost approach optimises enzyme addition. Feed Tech. 2, 29–31.
Purkarthofer, H., Sinner, M. and Steiner, W. (1993): Cellulase-free xylanase from Thermomyces lanuginosus: optimization of production in submerged and solid-state culture. Enzyme Microb. Technol. 15, 677–682.
Purkarthofer, H. and Steiner, W. (1995): Induction of endo-beta-Xylanase in the fungus Thermomyces lanuginosus. Enzyme Microb. Technol. 17, 114–118.
Schülien, M., Heldt-Hansen, H. P. and Dalbqge, H. (1992): Xylanase corresponding recombinant DNA sequence, xylanase containing agent, and use of the agent. WO Patent 92, 17573.
Silversides, F. G. (1999): Soluble non-starch polysaccharides, enzymes, and gut viscosity - is there a connection? World Poultry 15, 17–18.
Van de Mierop, L. and Ghesquiere, H. (1998): Enzymes have a long life ahead. World Poultry 14, 16–18.