Identification of Trypanosoma evansi by DNA hybridisation using a non-radioactive probe generated by arbitrary primer PCR: Short communication

S. H. Basagoudanavar; J. R. Rao; Swati Omanwar; A. K. Tiwari; R. K. Singh; R. S. Kataria; G. Butchaiah

doi:10.1556/004.49.2001.2.8

Acta Veterinaria Hungarica

Volume/Issue:: Volume 49: Issue 2

Identification of Trypanosoma evansi by DNA hybridisation using a non-radioactive probe generated by arbitrary primer PCR: Short communication

Authors:

S. H. Basagoudanavar

S. H. Basagoudanavar National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India

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J. R. Rao

J. R. Rao Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India

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jrrao@ivri.up.nic.in

Swati Omanwar

Swati Omanwar National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India

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A. K. Tiwari

A. K. Tiwari National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India

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R. K. Singh

R. K. Singh National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India

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R. S. Kataria

R. S. Kataria National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India

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, and

G. Butchaiah

G. Butchaiah National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India

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Pages:: 191–195

Online Publication Date:: Apr 2001

Publication Date:: 01 Apr 2001

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/004.49.2001.2.8

Keywords:: AP-PCR; non-isotopic probe

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A highly reproducible, dominant, monomorphic fragment of 473 base pair (bp) amplified from the genome of Trypanosoma evansi by arbitrary primer — polymerase chain reaction (AP-PCR) was labelled with digoxigenin and investigated for its potential as DNA probe. Dot-blot hybridisation of total genomic DNA with the probe proved useful in detecting bubaline, cameline and equine strains of T. evansi down to 10 pg of parasite template DNA. No cross-hybridisation was seen with Babesia bigemina, Theileria annulata and the bubaline host DNA. This probe may facilitate laboratory identification of T. evansi in developing countries, without the inherent risk associated with radioisotopes.

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Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

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Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language	English
Size	A4
Year of Foundation	1951
Volumes per Year	1
Issues per Year	4
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ISSN	0236-6290 (Print)
ISSN	1588-2705 (Online)