Acta Veterinaria Hungarica
Volume/Issue:
Volume 49: Issue 3
Resolution of parentage in dogs by examination of microsatellites after death of putative sire: Case report
Authors: Z. Pádár 1 , B. Egyed 1 , K. Kontadakis 2 , L. Zöldág 2 , and S. Fekete 2
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  • 1 DNA Laboratory, P.O. Box 314/4, H-1903 Budapest, Hungary
  • 2 Szent Istán University, Budapest, Hungary
DOI:
https://doi.org/10.1556/004.49.2001.3.2
Page Count:
269–273
Publication Date:
01 Aug 2001
Online Publication Date:
Aug 2001
Article Category:
Research Article
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A case of disputed paternity in dogs is reported. DNA examinations were carried out from hair samples of the individuals several months after the death of the putative sire. Ten short tandem repeat (STR) loci were analysed by fluorescence- labelled multiplex PCR using ABI PRISM 310 Genetic Analyser. Based on the results the candidate sire was included in the pedigree records as the biological sire. In spite o f the genetic homogeneity of pedigree dogs due to inbreeding, canine microsatellites can provide an adequate basis for assigning paternity in pure breeds.

  • FBI Laboratory (1994): PCR-Based Typing Protocols. FBI Laboratory, Washington, D.C.

  • Francisco, L. V., Langston, A. A., Mellersh, C. S., Neal, C. L. and Ostrander, E. A. (1996): A class of highly polymorphic tetranucleotide repeats for canine genetic mapping. Mammalian Genome 7, 359–362.

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    • Export Citation

  • Fredholm, M. and Winters, A. K. (1996): Efficient resolution of parentage in dogs by amplification ofmicrosatellites. Anim. Genet. 27, 19–23.

    • Search Google Scholar
    • Export Citation

  • Shutler, G. G., Gagnon, P., Verret, G., Kalyn, H., Korkosh, S., Johnston, E. and Halverson, J. (1999): Removal of a PCR inhibitor and resolution of DNA STR types in mixed humancanine stains from a five year old case. J. Forensic Sci. 44, 623–626.

    • Search Google Scholar
    • Export Citation

  • Vriesendorp, H. M., D-Duyzer-Den Hartog, B., Smid-Mercx, B. M. J. and Westbroek, D. L. (1974): Immunogenetic markers in canine paternity cases. J. Small Anim. Pract. 15, 693–699.

    • Search Google Scholar
    • Export Citation

  • Zajc, I., Mellersh, C. S., Kelly, E. P. and Sampson, J. (1994): A new method of paternity testing for dogs, based on microsatellite sequences. Vet. Rec. 135, 545–547.

    • Search Google Scholar
    • Export Citation

  • Zajc, I., Mellersh, C. S. and Sampson, J. (1997): Variability of canine microsatellites within and between different dog breeds. Mammalian Genome 8, 182–185.

    • Search Google Scholar
    • Export Citation
Cover Acta Veterinaria Hungarica
Acta Veterinaria Hungarica
Print ISSN:
0236-6290
Online ISSN:
1588-2705
Keywords:
Dog; microsatellites; parentage control

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