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  • 1 Department of General and Thoracic Surgery, Semmelweis Teaching Hospital H-3529 Miskolc, Csabai kapu 9-11, Hungary
  • 2 József Fodor' National Center for Public Health Budapest, Hungary
  • 3 Department of Medical Microbiology and Immunology, University of Pécs Pécs, Hungary
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Besides the well-known O157:H7 clone causing enterohaemorrhagic colitis and haemolytic uraemic syndrome in Europe, Japan and North America, the number of Escherichia coli isolates with non-motile (NM) phenotype has considerably increased. We supposed that spontaneous antibiotic resistance mutation could cause this phenotypic change. To model our hypothesis we isolated rifampicin- (Rif) and ampicillin- (Amp) resistant mutants from E. coli O157:H7 prototype strains 7785 and EDL933. Among Rif r mutants we could isolate strains with no or reduced motility, while the Ampr mutants became hypermotile. The biochemical profile of the mutants had not changed but phage sensitivity and generation time of the mutants were altered. Among the representative strains we did not find polymorphism with Southern blot analysis and no polymorphism was found in the fliC gene of the mutants. The described characteristics have proven to be stable. In a mice virulence assay by intravenous infections the virulence of the derivatives was also found to be changed. In summary, we found that the antibiotic-resistant phenotype in E. coli O157:H7 was coexpressed with several other phenotypic changes including motility and virulence. It can be assumed that expression of the involved phenotypes may be under the influence of a common regulatory cascade. Further work is needed to identify the components and mechanism of this regulatory system.

  • O' Brien, A. D., Newland, J. W., Miller, S. F., Holmes, R. K., Smith, H. W. and Formal, S. B. 1983: Escherichia coli O157:H7 strains associated with haemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (Shiga)-like cytotoxin. Lancet 1, 702.

    'Escherichia coli O157:H7 strains associated with haemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (Shiga)-like cytotoxin. ' () 1 Lancet : 702.

    • Search Google Scholar
  • Perna, N. T., Plunkett, G. 3rd, Burland, V., Mau, B., Glasner, J. D., Rose, D. J., Mayhew, G. F., Evans, P. S., Gregor, J., Kirkpatrick, H. A., Posfai, G., Hackett, J., Klink, S., Boutin, A., Shao, Y., Miller, L., Grotbeck, E. J., Davis, N. W., Lim, A., Dimalanta, E. T., Potamousis, K. D., Apodaca, J., Anantharaman, T. S., Lin, J., Yen, G., Schwartz, D. C., Welch, R. A. and Blattner, F. R. 2001: Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 409, 529-533.

    'Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. ' () 409 Nature : 529 -533.

    • Search Google Scholar
  • Reid, S. D., Selander, R. K. and Whittam, T. S. 1999: Sequence diversity of flagellin (fliC) alleles in pathogenic Escherichia coli . J. Bacteriol. 181, 153-160.

    'Sequence diversity of flagellin (fliC) alleles in pathogenic Escherichia coli . ' () 181 J. Bacteriol. : 153 -160.

    • Search Google Scholar
  • Riley, L. W., Remis, R. S., Helgerson, S. D., McGee, H. B., Wells, J. G., Davis, B. R., Herbert, R. J., Olcott, H. M., Johnson, L. M., Hargett, N. T., Blake, P. A. and Cohen, M. L. 1983: Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Eng. J. Med. 308, 681-685.

    'Hemorrhagic colitis associated with a rare Escherichia coli serotype. ' () 308 N. Eng. J. Med. : 681 -685.

    • Search Google Scholar
  • Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989: Molecular Cloning: A Laboratory Manual. Cold Spring Harbour Laboratory Press, Cold Spring Harbour, New York.

    Molecular Cloning: A Laboratory Manual , ().

  • Sperandio, V., Mellies, J. L., Nguyen, W., Shin, S. and Kaper, J. B. 1999: Quorum sensing controls expression of the type III secretion gene transcription and protein secretion in enterohemorrhagic and enteropathogenic Escherichia coli. Proc. Natl. Acad. Sci. USA 96, 15196-15201.

    'Quorum sensing controls expression of the type III secretion gene transcription and protein secretion in enterohemorrhagic and enteropathogenic Escherichia coli. ' () 96 Proc. Natl. Acad. Sci. USA : 15196 -15201.

    • Search Google Scholar
  • Sperandio, V., Torres, A. G., Giron, J. A. and Kaper, J. B. 2001: Quorum sensing is a global regulatory mechanism in enterohemorrhagic Escherichia coli O157:H7. J. Bacteriol. 183, 5187-5197.

    'Quorum sensing is a global regulatory mechanism in enterohemorrhagic Escherichia coli O157:H7. ' () 183 J. Bacteriol. : 5187 -5197.

    • Search Google Scholar
  • Surette, M. G., Miller, M. B. and Bassler, B. L. 1999: Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi : a new family of genes responsible for autoinducer production. Proc. Natl. Acad. Sci. USA 96,1639-1644.

    'Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi : a new family of genes responsible for autoinducer production. ' () 96 Proc. Natl. Acad. Sci. USA : 1639 -1644.

    • Search Google Scholar
  • Tóth, I., Barrett, T. J., Cohen, M. L., Rumschlag, H. S., Green, J. H. and Wachsmuth, I. K. 1991: Enzyme-linked immunosorbent assay for products of the 60-megadalton plasmid of Escherichia coli serotype O157:H7. J. Clin. Microbiol. 29, 1016-1019.

    'Enzyme-linked immunosorbent assay for products of the 60-megadalton plasmid of Escherichia coli serotype O157:H7. ' () 29 J. Clin. Microbiol. : 1016 -1019.

    • Search Google Scholar
  • Tóth, I., Cohen, M. L., Rumschlag, H. S., Riley, L. W., White, E. H., Carr, J. H., Bond, W. W. and Wachsmuth, I. K. 1990: Influence of the 60-megadalton plasmid on adherence of Escherichia coli O157:H7 and genetic derivatives. Infect. Immun. 58, 1223-1231.

    'Influence of the 60-megadalton plasmid on adherence of Escherichia coli O157:H7 and genetic derivatives. ' () 58 Infect. Immun. : 1223 -1231.

    • Search Google Scholar
  • Wells, J. G., Davis, B. R., Wachsmuth, I. K., Riley, L. W., Remis, R. S., Sokolow, R. and Morris, G. K. 1983: Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J. Clin. Microbiol. 18, 512-520.

    'Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. ' () 18 J. Clin. Microbiol. : 512 -520.

    • Search Google Scholar
  • Yamamori, T., Ito, K., Yura, T., Suzuki, T. and Iino, T. 1977: Ribonucleic acid polymerase mutants of Escherichia coli defective in flagella formation. J. Bact. 132, 254-261.

    'Ribonucleic acid polymerase mutants of Escherichia coli defective in flagella formation. ' () 132 J. Bact. : 254 -261.

    • Search Google Scholar
  • Gardel, C. L. and Mekalanos, J. J. 1996: Alterations in Vibrio cholerae motility phenotypes correlate with changes in virulence factor expression. Infect. Immun. 64, 2246-2255.

    'Alterations in Vibrio cholerae motility phenotypes correlate with changes in virulence factor expression. ' () 64 Infect. Immun. : 2246 -2255.

    • Search Google Scholar
  • Griffin, P. M. and Tauxe, R. V. 1991: The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and associated hemolytic uremic syndrome. Epid. Rev. 13, 60-98.

    'The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and associated hemolytic uremic syndrome. ' () 13 Epid. Rev. : 60 -98.

    • Search Google Scholar
  • Gunzer, F., Böhm, H., Rüssmann, H., Bitzan, M., Aleksic, S. and Karch, H. 1992: Molecular detection of sorbitol-fermenting Escherichia coli O157 in patients with hemolytic-uremic syndrome. J. Clin. Microbiol. 30, 1807-1810.

    'Molecular detection of sorbitol-fermenting Escherichia coli O157 in patients with hemolytic-uremic syndrome. ' () 30 J. Clin. Microbiol. : 1807 -1810.

    • Search Google Scholar
  • Hammarström, E. 1949: Phage-typing of Shigella sonnei. Acta Med. Scand. (Suppl. 233) 133, 1-132.

    'Phage-typing of Shigella sonnei. ' () 133 Acta Med. Scand. (Suppl. 233) : 1 -132.

  • Jin, D. J. and Gross, C. A. 1988: Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. J. Mol. Biol. 202, 45-58.

    'Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. ' () 202 J. Mol. Biol. : 45 -58.

    • Search Google Scholar
  • Jin, D. J. and Gross, C. A. 1989: Characterization of the pleiotropic phenotypes of rifampinresistant rpoB mutants of Escherichia coli. J. Bacteriol. 171, 5229-5231.

    'Characterization of the pleiotropic phenotypes of rifampinresistant rpoB mutants of Escherichia coli. ' () 171 J. Bacteriol. : 5229 -5231.

    • Search Google Scholar
  • Karch, H. and Bielaszewska, M. 2001: Sorbitol-fermenting Shiga toxin-producing Escherichia coli O157:H(-) strains: epidemiology, phenotypic and molecular characteristics, and microbiological diagnosis. J. Clin. Microbiol. 39, 2043-2049.

    'Sorbitol-fermenting Shiga toxin-producing Escherichia coli O157:H(-) strains: epidemiology, phenotypic and molecular characteristics, and microbiological diagnosis. ' () 39 J. Clin. Microbiol. : 2043 -2049.

    • Search Google Scholar
  • Karch, H., Böhm, H., Schmidt, H., Gunzer, F., Aleksic, S. and Heesemann, J. 1993: Clonal structure and pathogenicity of Shiga-like toxin-producing, sorbitol-fermenting Escherichia coli O157:H-. J. Clin. Microbiol. 31, 1200-1205.

    'Clonal structure and pathogenicity of Shiga-like toxin-producing, sorbitol-fermenting Escherichia coli O157:H-. ' () 31 J. Clin. Microbiol. : 1200 -1205.

    • Search Google Scholar
  • Laemmli, U. K. 1970: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.

    'Cleavage of structural proteins during the assembly of the head of bacteriophage T4. ' () 227 Nature : 680 -685.

    • Search Google Scholar
  • Milch, H. and Gyenes, M. 1972: Subdivision and correlation studies of serologically grouped Escherichia coli strains by phage typing, colicinogeny, lysogeny and biochemical test. Acta Microbiol. Acad. Sci. Hung. 19, 213-244.

    'Subdivision and correlation studies of serologically grouped Escherichia coli strains by phage typing, colicinogeny, lysogeny and biochemical test. ' () 19 Acta Microbiol. Acad. Sci. Hung. : 213 -244.

    • Search Google Scholar
  • Morooka, T., Umeda, A. and Amako, K. 1985: Motility as an intestinal colonization factor for Camphylobacter jejuni . J. Gen. Microbiol. 131, 1973-1980.

    'Motility as an intestinal colonization factor for Camphylobacter jejuni . ' () 131 J. Gen. Microbiol. : 1973 -1980.

    • Search Google Scholar
  • Brunder, W., Schmidt, H. and Karch, H. 1997: EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V. Mol. Microbiol. 24, 767-778.

    'EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V. ' () 24 Mol. Microbiol. : 767 -778.

    • Search Google Scholar
  • Caprioli, A. and Tozzi, A. E. 1998: Epidemiology of Shiga toxin-producing Escherichia coli infections in continental Europe. In: Kaper, J. B. and O' Brien, A. D. (eds) Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains. American Society for Microbiology, Washington, D.C., pp. 38-48.

  • China, B., Pirson, V. and Mainil, J. 1996: Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes. Appl. Environ. Microbiol. 62, 3462-3465.

    'Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes. ' () 62 Appl. Environ. Microbiol. : 3462 -3465.

    • Search Google Scholar
  • Fields, P. I., Blom, K., Hughes, H. J., Helsel, L. O., Feng, P. and Swaminathan, B. 1997: Molecular characterization of gene encoding H antigen in Escherichia coli and development of PCR-restriction length polymorphism test for identification of E. coli O157:H7 and O157:NM. J. Clin. Microbiol. 35, 1066-1070.

    'Molecular characterization of gene encoding H antigen in Escherichia coli and development of PCR-restriction length polymorphism test for identification of E. coli O157:H7 and O157:NM. ' () 35 J. Clin. Microbiol. : 1066 -1070.

    • Search Google Scholar
  • Fuchs, S., Muhldorfer, I., Donohue-Rolfe, A., Kerenyi, M., Emody, L., Alexiev, R., Nenkov, P. and Hacker, J. 1999: Influence of RecA on in vivo virulence and Shiga toxin 2 production in Escherichia coli pathogens. Microb. Pathog. 27, 13-23.

    'Influence of RecA on in vivo virulence and Shiga toxin 2 production in Escherichia coli pathogens. ' () 27 Microb. Pathog. : 13 -23.

    • Search Google Scholar
  • Farmer, J. J. 3rd and Davis, B. D. 1985: H7 antiserum-sorbitol fermentation medium: a single tube screening medium for detecting Escherichia coli O157:H7 associated with hemorrhagic colitis. J. Clin. Microbiol. 22, 620-625.

    'H7 antiserum-sorbitol fermentation medium: a single tube screening medium for detecting Escherichia coli O157:H7 associated with hemorrhagic colitis. ' () 22 J. Clin. Microbiol. : 620 -625.

    • Search Google Scholar
  • Farris, M., Grant, A., Richardson, T. B. and O'Connor, C. D. 1998: BipA: a tyrosinephosphorylated GTPase that mediates interaction between enteropathogenic Escherichia coli (EPEC) and epithelial cells. Mol. Microbiol. 28, 265-279.

    'BipA: a tyrosinephosphorylated GTPase that mediates interaction between enteropathogenic Escherichia coli (EPEC) and epithelial cells. ' () 28 Mol. Microbiol. : 265 -279.

    • Search Google Scholar
  • LeClerc, J. E., Li, B., Payne, W. L. and Cebula, T. A. 1996: High mutation frequencies among Escherichia coli and Salmonella pathogens. Science 274, 1208-1211.

    'High mutation frequencies among Escherichia coli and Salmonella pathogens. ' () 274 Science : 1208 -1211.

    • Search Google Scholar
  • Liesegang, A., Sachse, U., Prager, R., Claus, H., Steinruck, H., Aleksic, S., Rabsch, W., Voigt, W., Fruth, A., Karch, H., Bockemuhl, J. and Tschape, H. 2000: Clonal diversity of Shiga toxin-producing Escherichia coli O157:H7/H- in Germany - a ten-year study. Int. J. Med. Microbiol. 290, 269-278.

    'Clonal diversity of Shiga toxin-producing Escherichia coli O157:H7/H- in Germany - a ten-year study. ' () 290 Int. J. Med. Microbiol. : 269 -278.

    • Search Google Scholar
  • Bielaszewska, M., Schmidt, H., Karmali, M. A., Khakhria, R., Janda, J., Blahova, K. and Karch, H. 1998: Isolation and characterization of sorbitol-fermenting Shiga toxin (verocytotoxin)- producing Escherichia coli O157:H- strains in the Czech Republic. J. Clin. Microbiol. 36, 2135-2137.

    'Isolation and characterization of sorbitol-fermenting Shiga toxin (verocytotoxin)- producing Escherichia coli O157:H- strains in the Czech Republic. ' () 36 J. Clin. Microbiol. : 2135 -2137.

    • Search Google Scholar
  • Feldman, M., Bryan, R., Rajan, S., Scheffler, L., Brunnert, S., Tang, H. and Prince, A. 1998: Role of flagella in pathogenesis of Pseudomonas aeruginosa pulmonary infection. Infect. Immun. 66, 43-51.

    'Role of flagella in pathogenesis of Pseudomonas aeruginosa pulmonary infection. ' () 66 Infect. Immun. : 43 -51.

    • Search Google Scholar
  • Allison, C., Emody, L., Coleman, N. and Hughes, C. 1994: The role of swarm cell differentiation and multicellular migration in the uropathogenicity of Proteus mirabilis . J. Infect Dis. 69, 1155-1588.

    'The role of swarm cell differentiation and multicellular migration in the uropathogenicity of Proteus mirabilis . ' () 69 J. Infect Dis. : 1155 -1588.

    • Search Google Scholar
  • Morschhäuser, J., Vetter, V., Emody, L. and Hacker, J. 1994: Adhesin regulatory genes within large, unstable DNA regions of pathogenic Escherichia coli : cross-talk between different adhesin gene clusters. Mol. Microbiol. 11, 555-566.

    'Adhesin regulatory genes within large, unstable DNA regions of pathogenic Escherichia coli : cross-talk between different adhesin gene clusters. ' () 11 Mol. Microbiol. : 555 -566.

    • Search Google Scholar
  • Feng, P., Lampel, K. A., Karch, H. and Whittam, T. S. 1998: Genotypic and phenotypic changes in the emergence of Escherichia coli O157:H7. J. Infect. Dis. 177, 1750-1753.

    'Genotypic and phenotypic changes in the emergence of Escherichia coli O157:H7. ' () 177 J. Infect. Dis. : 1750 -1753.

    • Search Google Scholar
  • Bachman, B. J. 1996: Derivations, and genotypes of some mutant derivatives of Escherichia coli K-12. In: Neihardt, F. C., Curtiss III., R., Ingraham, J. L., Lin, E. C., Low, K. B., Magassanik, B., Reznikoff, W. S., Riley, M., Schaechter, M. and Umbarger, H. E. (eds) Escherichia coli and Salmonella: Cellular and Molecular Biology. 2nd edition. ASM Press, Washington, D.C. pp. 2460-2488.

    Derivations, and genotypes of some mutant derivatives of Escherichia coli K-12 , () 2460 -2488.

    • Search Google Scholar

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