View More View Less
  • 1 Department of Anatomy, Faculty of Veterinary Medicine, Mansoura University Mansoura, Egypt
  • 2 Department and Clinic for Obstetrics and Reproduction, Faculty of Veterinary Science, Szent István University Budapest, Hungary
  • 3 Department of Physiology and Biochemistry, Faculty of Veterinary Science, Szent István University H-1400 Budapest, P.O. Box 2, Hungary
  • 4 Department of Physiology and Biochemistry, Faculty of Veterinary Science, Szent István University H-1400 Budapest, P.O. Box 2, Hungary
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

Leptin and leptin receptor were studied in the mammary gland of non-pregnant dry and lactating cows. Using RT-PCR it was demonstrated that leptin and its short (Ob-Ra) and long (Ob-Rb) receptor isoforms are expressed both in the dry and the lactating mammary gland tissue. Tissue distribution of leptin and its receptor mRNA transcripts were examined by insitu hybridisation, while the leptin protein was localised by immunohistochemistry. Although in situ hybridisation is semiquantitative, our morphological data suggest that the epithelial leptin mRNA expression of the lactating gland is higher than that of the dry gland. To compare the leptin mRNA levels between dry and lactating udders competitive PCR was used, which showed no difference in leptin expression for the whole mammary tissues. The lack of difference in total leptin mRNA levels is explained by the high adipose tissue content of the dry mammary gland. Leptin and its receptor transcripts are expressed mainly in the epithelial cells of lactating cows, while in dry mammary tissue the signal is found in the stromal tissues as well. The results provide additional evidence that locally produced leptin takes part in the regulation and maintenance of mammary epithelial cell activity.

  • Yanagihara, N., Utsunomiya, K., Cheah, T. B., Hirano, H., Kajiwara, K., Hara, K., Nakamura, E., Toyohira, Y., Uezono, Y., Ueno, S. and Izumi, F. (2000): Characterization and functional role of leptin receptor in bovine adrenal medullary cells. Biochem. Pharmacol. 59, 1141-1145.

    'Characterization and functional role of leptin receptor in bovine adrenal medullary cells ' () 59 Biochem. Pharmacol. : 1141 -1145.

    • Search Google Scholar
  • Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L. and Friedman, J. M. (1994): Positional cloning of the mouse obese gene and its human homologue. Nature 372, 425-432.

    'Positional cloning of the mouse obese gene and its human homologue ' () 372 Nature : 425 -432.

    • Search Google Scholar
  • Ahima, R. S. and Flier, J. S. (2000): Adipose tissue as an endocrine organ. Trends Endocrinol. Metabol. 11, 327-332.

    'Adipose tissue as an endocrine organ ' () 11 Trends Endocrinol. Metabol. : 327 -332.

  • Aoki, N., Kawamura, M. and Matsuda, T. (1999): Lactation-dependent down regulation of leptin production in mouse mammary gland. Biochim. Biophys. Acta 1427, 298-306.

    'Lactation-dependent down regulation of leptin production in mouse mammary gland ' () 1427 Biochim. Biophys. Acta : 298 -306.

    • Search Google Scholar
  • Bornstein, S. R., Abu-Asab, M., Glasow, A., Path, G., Hauner, H., Tsokos, M., Chrousos, G. P. and Scherbaum, W. A. (2000): Immunohistochemical and ultrastructural localization of leptin and leptin receptor in human white adipose tissue and differentiating human adipose cells in primary culture. Diabetes 49, 532-538.

    'Immunohistochemical and ultrastructural localization of leptin and leptin receptor in human white adipose tissue and differentiating human adipose cells in primary culture ' () 49 Diabetes : 532 -538.

    • Search Google Scholar
  • Considine, R. V. and Caro, J. F. (1997): Leptin and the regulation of body weight Int. J. Biochem. Cell Biol. 29, 1255-1272.

    'Leptin and the regulation of body weight Int ' () 29 J. Biochem. Cell Biol. : 1255 -1272.

    • Search Google Scholar
  • Dyer, C. J., Simmons, J. M., Matteri, R. L. and Keisler, D. H. (1997): Leptin receptor mRNA is expressed in ewe anterior pituitary and adipose tissues and is differentially expressed in hypothalamic regions of well-fed and feed-restricted ewes. Domest. Anim. Endocrinol. 14, 119-128.

    'Leptin receptor mRNA is expressed in ewe anterior pituitary and adipose tissues and is differentially expressed in hypothalamic regions of well-fed and feed-restricted ewes ' () 14 Domest. Anim. Endocrinol. : 119 -128.

    • Search Google Scholar
  • Ehrhardt, R. A., Slepetis, R. M., Siegal-Willott, J., Van Amburgh, M. E., Bell, A. W. and Boisclair, Y. R. (2000): Development of a specific radioimmunoassay to measure physiological changes of circulating leptin in cattle and sheep. J. Endocrinol. 166, 519-528.

    'Development of a specific radioimmunoassay to measure physiological changes of circulating leptin in cattle and sheep ' () 166 J. Endocrinol. : 519 -528.

    • Search Google Scholar
  • Estienne, M. J., Harper, A. F., Barb, C. R. and Azain, M. J. (2000): Concentrations of leptin in serum and milk collected from lactating sows differing in body condition. Domest. Anim. Endocrinol. 19, 275-280. 110

    'Concentrations of leptin in serum and milk collected from lactating sows differing in body condition ' () 19 Domest. Anim. Endocrinol. : 275 -280.

    • Search Google Scholar
  • SAYED-AHMED et al. Acta Veterinaria Hungarica 52, 2004.

  • Baumann, H., Morella, K. K., White, D. W., Dembski, M., Bailon, P. S., Kim, H., Lai, C. F. and Tartaglia, L. A. (1996): The full-length leptin receptor has signaling capabilities of interleukin 6-type cytokine receptors. Proc. Natl. Acad. Sci. USA 93, 8374-8378.

    'The full-length leptin receptor has signaling capabilities of interleukin 6-type cytokine receptors ' () 93 Proc. Natl. Acad. Sci. USA : 8374 -8378.

    • Search Google Scholar
  • Bonnet, M., Gourdou, I., Leroux, C., Chilliard, Y. and Djiane, J. (2002): Leptin expression in the ovine mammary gland: putative sequential involvement of adipose, epithelial, and myoepithelial cells during pregnancy and lactation. J. Anim. Sci. 80, 723-728.

    'Leptin expression in the ovine mammary gland: putative sequential involvement of adipose, epithelial, and myoepithelial cells during pregnancy and lactation ' () 80 J. Anim. Sci. : 723 -728.

    • Search Google Scholar
  • Casabiell, X., Pineiro, V., Tome, M. A., Peino, R., Dieguez, C. and Casanueva, F. F. (1997): Presence of leptin in colostrum and/or breast milk from lactating mothers: a potential role in the regulation of neonatal food intake. J. Clin. Endocrinol. Metab. 82, 4270-4273.

    'Presence of leptin in colostrum and/or breast milk from lactating mothers: a potential role in the regulation of neonatal food intake ' () 82 J. Clin. Endocrinol. Metab. : 4270 -4273.

    • Search Google Scholar
  • Chilliard, Y., Bonnet, M., Delavaud, C., Faulconnier, Y., Leroux, C., Djiane, J. and Bocquier, F. (2001): Leptin in ruminants. Gene expression in adipose tissue and mammary gland, and regulation of plasma concentration. Domest. Anim. Endocrinol. 21, 271-295.

    'Leptin in ruminants. Gene expression in adipose tissue and mammary gland, and regulation of plasma concentration ' () 21 Domest. Anim. Endocrinol. : 271 -295.

    • Search Google Scholar
  • Fei, H., Okano, H. J., Li, C., Lee, G. H., Zhao, C., Darnell, R. and Friedman, J. M. (1997): Anatomic localization of alternatively spliced leptin receptors (Ob-R) in mouse brain and other tissues. Proc. Natl. Acad. Sci. USA 94, 7001-7005.

    'Anatomic localization of alternatively spliced leptin receptors (Ob-R) in mouse brain and other tissues ' () 94 Proc. Natl. Acad. Sci. USA : 7001 -7005.

    • Search Google Scholar
  • Halaas, J. L., Gajiwala, K. S., Maffei, M., Cohen, S. L., Chait, B. T., Rabinowitz, D., Lallone, R. L., Burley, S. K. and Friedman, J. M. (1995): Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543-546.

    'Weight-reducing effects of the plasma protein encoded by the obese gene ' () 269 Science : 543 -546.

    • Search Google Scholar
  • Houseknecht, K. L. and Portocarrero, C. P. (1998): Leptin and its receptors: regulators of wholebody energy homeostasis. Domest. Anim. Endocrinol. 15, 457-475.

    'Leptin and its receptors: regulators of wholebody energy homeostasis ' () 15 Domest. Anim. Endocrinol. : 457 -475.

    • Search Google Scholar
  • Houseknecht, K. L., Baile, C. A., Matteri, R. L. and Suprlock, M. E. (1998): The biology of leptin: a review. J. Anim. Sci. 76, 1405-1420.

    'The biology of leptin: a review ' () 76 J. Anim. Sci. : 1405 -1420.

  • Knoll, J. H. M. and Lichter, P. (1995): In situ hybridization and detection using nonisotopic probes. In: Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. (eds) Current Protocols in Molecular Biology. Volume 2. John Wiley & Sons, Inc., Etobicoke, Ontario, Canada. pp. 14.7.1-14.7.11.

    In situ hybridization and detection using nonisotopic probes , () 14.7.1 -14.7.11.

  • Laud, K., Gourdou, I., Belair, L., Keisler, D. H. and Djiane, J. (1999): Detection and regulation of leptin receptor mRNA in ovine mammary epithelial cells during pregnancy and lactation. FEBS Lett. 463, 194-198.

    'Detection and regulation of leptin receptor mRNA in ovine mammary epithelial cells during pregnancy and lactation ' () 463 FEBS Lett. : 194 -198.

    • Search Google Scholar
  • Hovey, R. C., MacKenzie, D. D. and McFadden, T. B. (1998): The proliferation of mouse mammary epithelial cells in response to specific mitogens is modulated by the mammary fat pad in vitro. In Vitro Cell. Dev. Biol. Anim. 34, 385-392.

    'The proliferation of mouse mammary epithelial cells in response to specific mitogens is modulated by the mammary fat pad in vitro. ' () 34 In Vitro Cell. Dev. Biol. Anim. : 385 -392.

    • Search Google Scholar
  • Hu, X., Juneja, S. C., Maihle, N. J. and Cleary, M. P. (2002): Leptin - a growth factor in normal and malignant breast cells and for normal mammary gland development. J. Natl. Cancer Inst. 94, 1704-1711.

    'Leptin - a growth factor in normal and malignant breast cells and for normal mammary gland development ' () 94 J. Natl. Cancer Inst. : 1704 -1711.

    • Search Google Scholar
  • Laud, K., Gourdou, I., Pessemesse, L., Peyrat, J. P. and Djiane, J. (2002): Identification of leptin receptors in human breast cancer: functional activity in the T47-D breast cancer cell line. Mol. Cell. Endocrinol. 188, 219-226.

    'Identification of leptin receptors in human breast cancer: functional activity in the T47-D breast cancer cell line ' () 188 Mol. Cell. Endocrinol. : 219 -226.

    • Search Google Scholar
  • Machinal-Quelin, F., Dieudonne, M. N., Leneveu, M. C., Pecquery, R. and Giudicelli, Y. (2002): Proadipogenic effect of leptin on rat preadipocytes in vitro: activation of MAPK and STAT3 signaling pathways. Am. J. Physiol. Cell Physiol. 282, 853-863.

    'Proadipogenic effect of leptin on rat preadipocytes in vitro: activation of MAPK and STAT3 signaling pathways ' () 282 Am. J. Physiol. Cell Physiol. : 853 -863.

    • Search Google Scholar
  • Mercer, J. G., Hoggard, N., Williams, L. M., Lawrence, C. B., Hannah, L. T. and Trayhurn, P. (1996): Localization of leptin receptor mRNA and the long form splice variant (Ob-Rb) in mouse hypothalamus and adjacent brain regions by in situ hybridization. FEBS Lett. 387, 113-116.

    'Localization of leptin receptor mRNA and the long form splice variant (Ob-Rb) in mouse hypothalamus and adjacent brain regions by in situ hybridization ' () 387 FEBS Lett. : 113 -116.

    • Search Google Scholar
  • Moschos, S., Chan, J. L. and Mantzoros, C. S. (2002): Leptin and reproduction: a review. Fertil. Steril. 77, 433-444.

    'Leptin and reproduction: a review ' () 77 Fertil. Steril. : 433 -444.

  • Maffei, M., Halaas, J., Ravussin, E., Pratley, R. E., Lee, G. H., Zhang, Y., Fei, H., Kim, S., Lallone, R., Ranganathan, S., Kern, P. K. and Friedman, J. M. (1995): Leptin levels in human and rodent: Measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat. Med. 11, 1155-1161.

    'Leptin levels in human and rodent: Measurement of plasma leptin and ob RNA in obese and weight-reduced subjects ' () 11 Nat. Med. : 1155 -1161.

    • Search Google Scholar
  • Mounzih, K., Qiu, J., Ewart-Toland, A. and Chehab, F. F. (1998): Leptin is not necessary for gestation and parturition but regulates maternal nutrition via a leptin resistance state. Endocr inology 139, 5259-5262.

    'Leptin is not necessary for gestation and parturition but regulates maternal nutrition via a leptin resistance state ' () 139 Endocr inology : 5259 -5262.

    • Search Google Scholar
  • Silva, L. F., VandeHaar, M. J., Weber Nielsen, M. S. and Smith, G. W. (2002): Evidence for a local effect of leptin in bovine mammary gland. J. Dairy Sci. 85, 3277-3286.

    'Evidence for a local effect of leptin in bovine mammary gland ' () 85 J. Dairy Sci. : 3277 -3286.

    • Search Google Scholar
  • Smith, J. L. and Sheffield, L. G. (2002): Production and regulation of leptin in bovine mammary epithelial cells. Domest. Anim. Endocrinol. 22, 145-154.

    'Production and regulation of leptin in bovine mammary epithelial cells ' () 22 Domest. Anim. Endocrinol. : 145 -154.

    • Search Google Scholar
  • Smith-Kirwin, S. M., O'Connor, D. M., Johnston, J., De Lancey, E., Hassink, S. G. and Funanage, V. L. (1998): Leptin expression in human mammary epithelial cells and breast milk. J. Clin. Endocrinol. Metab. 83, 1810-1813.

    'Leptin expression in human mammary epithelial cells and breast milk ' () 83 J. Clin. Endocrinol. Metab. : 1810 -1813.

    • Search Google Scholar
  • Sweeney, G. (2002): Leptin signalling. Cellular Signalling 14, 655-663.

    'Leptin signalling ' () 14 Cellular Signalling : 655 -663.

  • Tartaglia, L. A. (1997): The leptin receptor. J. Biol. Chem. 272, 6093-6096.

    'The leptin receptor ' () 272 J. Biol. Chem. : 6093 -6096.

  • Tu, H. M., Kim, S. W., Salvatore, D., Bartha, T., Legradi, G., Larsen, P. R. and Lechan, R. M. (1997): Regional distribution of type 2 thyroxine deiodinase messenger ribonucleic acid in rat hypothalamus and pituitary and its regulation by thyroid hormone. Endocrinol. 138, 3359-3368.

    'Regional distribution of type 2 thyroxine deiodinase messenger ribonucleic acid in rat hypothalamus and pituitary and its regulation by thyroid hormone ' () 138 Endocrinol. : 3359 -3368.

    • Search Google Scholar
  • Vernon, R. G., Denis, R. G. P. and Sørensen, A. (2001): Signals of adiposity. Domest. Anim. Endocrinol. 21, 197-214.

    'Signals of adiposity ' () 21 Domest. Anim. Endocrinol. : 197 -214.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 5 0
Jul 2020 5 0 0
Aug 2020 8 0 0
Sep 2020 22 0 0
Oct 2020 4 0 0
Nov 2020 5 1 0
Dec 2020 0 0 0