Detection of infectious laryngotracheitis virus (ILTV) by avidin-biotin complex (ABC) method was studied in trigeminal ganglia (TRG) during the acute and post-acute or latent period in chickens inoculated with an A96 strain of the antigen. TRG tissue samples were collected from uninoculated (10 chickens) and inoculated (30 chickens) animals at various intervals post inoculation (PI), and stained by the ABC method. The results indicated that no ILTV antigen was detected in the TRG between PI days 3 and 11. However, the antigen was detected in most of the chickens between PI days 13 and 41. It is concluded that the ABC method can be used successfully for the detection of ILTV antigens in TRG during post-acute or latent period.
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Jordan, F. T. W. (1996): Infectious laryngotracheitis. In: Jordan, F. T. W. and Pattison, M. (eds) Poultry Diseases. 4th edition. W. B. Saunders, London. pp. 173-177.
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Timurkaan, N., Yilmaz, F., Bulut, H., Ozer, H. and Bolat, Y. (2003): Pathological and immunohistochemical findings in broilers inoculated with a low virulent strain of infectious laryngotracheitis virus. J. Vet. Sci. 4, 175-180.
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Guy, J. S., Barnes, H. J. and Smith, L. G. (1992): Rapid diagnosis of infectious laryngotracheitis using a monoclonal antibody-based immunoperoxidase procedure. Avian Pathol. 21, 77-86.
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Haines, D. M. and Clark, E. G. (1991): Enzyme immunohistochemical staining of formalin-fixed tissues for diagnosis in veterinary pathology. Can. Vet. J. 32, 295-302.
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Bagust, T. J., Calnek, B. W. and Fahey, K. J. (1986): Gallid-1 herpesvirus in chickens. 3. Reinvestigation of the pathogenesis of infectious laryngotracheitis in acute and early post-acute respiratory disease. Avian Dis. 30, 179-190.
'Gallid-1 herpesvirus in chickens. 3. Reinvestigation of the pathogenesis of infectious laryngotracheitis in acute and early post-acute respiratory disease ' () 30 Avian Dis. : 179 -190.
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Bagust, T. J. and Johnson, M. A. (1995): Avian infectious laryngotracheitis virus: Virus-host interaction in relation to prospects for eradication. Avian Pathol. 24, 373-391.
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Bourne, J. A. (1985): Handbook of Immunoperoxidase Staining Methods. Immunochemistry Laboratory, DAKO Corporation, USA.
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