Comparison of selective media for the isolation of Rhodococcus equi and description of a new selective plating medium

L. Makrai; L. Fodor; I. Vendég; G. Szigeti; B. Dénes; J. Reiczigel; J. Varga

doi:10.1556/avet.53.2005.3.1

Jump to Content

Acta Veterinaria Hungarica

Volume/Issue:: Volume 53: Issue 3

Comparison of selective media for the isolation of Rhodococcus equi and description of a new selective plating medium

Authors:

L. Makrai

L. Makrai Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University H-1581 Budapest, P.O. Box 22, Hungary

Search for other papers by L. Makrai in
Current site
Google Scholar
PubMed

L. Fodor

L. Fodor Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University H-1581 Budapest, P.O. Box 22, Hungary

Search for other papers by L. Fodor in
Current site
Google Scholar
PubMed

I. Vendég

I. Vendég Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University H-1581 Budapest, P.O. Box 22, Hungary

Search for other papers by I. Vendég in
Current site
Google Scholar
PubMed

G. Szigeti

G. Szigeti Central Veterinary Institute Budapest, Hungary

Search for other papers by G. Szigeti in
Current site
Google Scholar
PubMed

B. Dénes

B. Dénes Central Veterinary Institute Budapest, Hungary

Search for other papers by B. Dénes in
Current site
Google Scholar
PubMed

J. Reiczigel

J. Reiczigel Department of Biomathematics and Informatics, Faculty of Veterinary Science, Szent István University Budapest, Hungary

Search for other papers by J. Reiczigel in
Current site
Google Scholar
PubMed

, and

J. Varga

J. Varga Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University H-1581 Budapest, P.O. Box 22, Hungary

Search for other papers by J. Varga in
Current site
Google Scholar
PubMed

View More View Less

Pages:: 275–285

Online Publication Date:: 19 Sep 2005

Publication Date:: 01 Aug 2005

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/avet.53.2005.3.1

Keywords:: Rhodococcus equi; Rhodococcus equi; Rhodococcus equi; Rhodococcus equi; isolation; new selective plating medium; TCP; contaminated samples

Restricted access

Purchase article

USD $30.00

1 year subscription (Individual Only)

USD $836.00

USD $30.00

Click here to view the full Terms and Conditions

USD $836.00

Click here to view the full Terms and Conditions

In order to improve the isolation rate of Rhodococcus equi from animals and soil, the efficacy of four previously described selective media (CAZ-NB, M3T, NANAT and TINSDALE) and that of four other media (NC, PNP, TCP and TVP) composed by us was compared and evaluated. Two selective plating media proved to be the best for the isolation of R. equi from contaminated samples. One of them was CAZ-NB containing ceftazidime, novobiocin and cycloheximide, while the other was the newly composed TCP containing trimethoprim, cefoperazone, polymyxin B, cycloheximide and potassium tellurite as selective components. These two media allowed the growth of at least 62-72% of R. equi present in the artificially contaminated samples, and the inhibition of unwanted contaminant bacteria and fungi was satisfactory with both media. TCP medium proved to be superior to CAZ-NB since the colony morphology of R. equi was much more characteristic (shiny, smooth, black colonies 3-5 mm in diameter) on it, and it inhibited the unwanted contaminant bacterial and fungal flora more effectively, especially in the case of faecal and soil samples. Therefore, TCP is recommended as a new, highly selective plating medium for the isolation of R. equi from contaminated samples.

Save
Cite
Email this content

Share Link

Copy this link, or click below to email it to a friend
Email this content
or copy the link directly:

https://akjournals.com/abstract/journals/004/53/3/article-p275.xml
The link was not copied. Your current browser may not support copying via this button.

Link copied successfully

Collapse
Expand

Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA

University of Veterinary Medicine,

H-1078 Budapest, István utca 2., Hungary

Phone: (36 20) 560 4183 (ed.-in-chief) or (36 1) 478 4100/8430 (editor)

E-mail: acta.veterinaria@univet.hu (ed.-in-chief)

Indexing and Abstracting Services:

Biological Abstracts
BIOSIS Previews
CAB Abstracts
Chemical Abstracts
Current Contents: Agriculture, Biology and Environmental Sciences
Elsevier Science Navigator
Focus On: Veterinary Science and Medicine
Global Health
Index Medicus
Index Veterinarius
Medline
Science Citation Index
Science Citation Index Expanded (SciSearch)
SCOPUS
The ISI Alerting Services
Zoological Abstracts

2024
Scopus
CiteScore
CiteScore rank
SNIP
Scimago
SJR index	0.272
SJR Q rank	Q2

2023
Web of Science
Journal Impact Factor	0.7
Rank by Impact Factor	Q3 (Veterinary Sciences)
Journal Citation Indicator	0.4
Scopus
CiteScore	1.8
CiteScore rank	Q2 (General Veterinary)
SNIP	0.39
Scimago
SJR index	0.258
SJR Q rank	Q3

Acta Veterinaria Hungarica
Publication Model	Hybrid
Submission Fee	none
Article Processing Charge	1100 EUR/article
Printed Color Illustrations	40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency	World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100%
Further Discounts	Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2025	Online subsscription: 832 EUR / 916 USD Print + online subscription: 960 EUR / 1054 USD
Subscription Information	Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title	Individual articles are sold on the displayed price.

Acta Veterinaria Hungarica
Language	English
Size	A4
Year of Foundation	1951
Volumes per Year	1
Issues per Year	4
Founder	Magyar Tudományos Akadémia
Founder's Address	H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher	Akadémiai Kiadó
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-6290 (Print)
ISSN	1588-2705 (Online)