View More View Less
  • 1 Clarigen Inc. Carlsbad, CA, USA
  • | 2 Clarigen Inc. Carlsbad, CA, USA
  • | 3 Veterinary Medical Research Institute of the Hungarian Academy of Sciences H-1581 Budapest, P.O. Box 18, Hungary
  • | 4 Second Department of Internal Medicine, Semmelweis University Budapest, Hungary
  • | 5 Veterinary Medical Research Institute of the Hungarian Academy of Sciences H-1581 Budapest, P.O. Box 18, Hungary
  • | 6 Veterinary Medical Research Institute of the Hungarian Academy of Sciences H-1581 Budapest, P.O. Box 18, Hungary
  • | 7 Veterinary Medical Research Institute of the Hungarian Academy of Sciences H-1581 Budapest, P.O. Box 18, Hungary
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

The capture of mycoplasmas (M. hominis, M. buccale, M. fermentans, M. bovis, M. synoviae, M. gallisepticum and M. arthritidis) based on lipid structures and adhesion molecules present in the mycoplasmal membrane was tested using different chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow and SulfEtox). All of the resins efficiently reduced mycoplasma concentrations in Phosphate Buffered Saline (PBS) and in Fetal Bovine Serum (FBS) by 3-8 logs in a few minutes. This technology could be used for removing mycoplasmas from tissue culture components such as serum, and for concentrating mycoplasmas in vaccine production.

  • Brade, H., Opal, S. M., Vogel, S. M. and Morrison, D. C. (eds) (1999): Endotoxin in Health and Disease. Marcel Dekker, New York.

    Endotoxin in Health and Disease , ().

  • Christensen, N. D., Reed, C. A., Culp, T. D., Hermonat, P. L., Howett, M. K., Anderson, R. A. and Zaneveld, J. D. (2001): Papillomavirus microbicidal activities of high-molecular weight cellulose sulfate, dextrane-sulfate and polystyrene sulfonate. Antimicrob. Agent Chemother. 45, 3427-3432.

    'Papillomavirus microbicidal activities of high-molecular weight cellulose sulfate, dextrane-sulfate and polystyrene sulfonate. ' () 45 Antimicrob. Agent Chemother. : 3427 -3432.

    • Search Google Scholar
  • Elfaki, M. G., Kleven, S. H., Jin, L. H. and Ragland, W. L. (1993): Protection against airsacculitis with sequential systemic and local immunization of chickens using killed Mycoplasma gallisepticum bacteria with iota carrageenan adjuvant. Vaccine 11, 311-317.

    'Protection against airsacculitis with sequential systemic and local immunization of chickens using killed Mycoplasma gallisepticum bacteria with iota carrageenan adjuvant. ' () 11 Vaccine : 311 -317.

    • Search Google Scholar
  • Ernø, H. and Stipkovits, L. (1973): Bovine mycoplasmas: Culture and biochemical studies. Acta Vet. Scand. 14, 436-449.

    'Bovine mycoplasmas: Culture and biochemical studies. ' () 14 Acta Vet. Scand. : 436 -449.

    • Search Google Scholar
  • Athamna, A., Kramer, M. R. and Kahane, I. (1996): Adherence of Mycoplasma pneumoniae to human alveolar macrophages. FEMS Immunol. Med. Microbiol. 15, 135-141.

    'Adherence of Mycoplasma pneumoniae to human alveolar macrophages. ' () 15 FEMS Immunol. Med. Microbiol. : 135 -141.

    • Search Google Scholar
  • Ben-Menachem, C., Zahringer, U. and Rottem, S. (2001): The phosphocholine motif in membranes of Mycoplasma fermentans strains. FEM Microbiol. Lett. 199, 137-141.

    'The phosphocholine motif in membranes of Mycoplasma fermentans strains. ' () 199 FEM Microbiol. Lett. : 137 -141.

    • Search Google Scholar
  • Kenny, G. E. (1985): Mycoplasma contamination of cell cultures. In: Gylstorff, I. (ed.) Infektionen durch Mycoplasmatales. VEB Gustav Fischer Verlag, Jena. pp. 210-227.

    Infektionen durch Mycoplasmatales , () 210 -227.

  • Kleven, S. H. (1990): Summary of discussion of Avian Mycoplasma Team, IRPCM, IOM. Avian Pathol. 19, 795-800.

    'Summary of discussion of Avian Mycoplasma Team, IRPCM, IOM. ' () 19 Avian Pathol. : 795 -800.

    • Search Google Scholar
  • Krivan, H. C., Olson, L. D., Barile, M. F., Ginsburg, V. and Roberts, D. D. (1989): Adhesion of Mycoplasma pneumoniae to sulfated glycolipids and inhibition by dextran sulfate. J. Biol. Chem. 264, 9283-9288.

    'Adhesion of Mycoplasma pneumoniae to sulfated glycolipids and inhibition by dextran sulfate. ' () 264 J. Biol. Chem. : 9283 -9288.

    • Search Google Scholar
  • Markham, P. F., Glew, M. D., Brandon, M. R., Walker, J. D. and Whithear, K. G.(1992): Characterization of a major hemagglutinin protein from Mycoplasma gallisepticum. Infect. Immun. 60, 3885-3891.

    'Characterization of a major hemagglutinin protein from Mycoplasma gallisepticum ' () 60 Infect. Immun. : 3885 -3891.

    • Search Google Scholar
  • Matsuda, K., Ishizuka, I. and Kasama, T. (1997): Structure of a novel phosphocholine-containing aminoglycoglycerolipid of Mycoplasma fermentans. Biochim. Biophys. Acta 1349, 1-12.

    'Structure of a novel phosphocholine-containing aminoglycoglycerolipid of Mycoplasma fermentans. ' () 1349 Biochim. Biophys. Acta : 1 -12.

    • Search Google Scholar
  • Muhlradt, P. F., Kiess, M., Meyer, H., Sussmuth, R. and Jung, G. (1997): Isolation, structure elucidation, and synthesis of a macrophage stimulatory lipopeptide from Mycoplasma fermentans acting at picomolar concentration. J. Exp. Med. 185, 1951-1958.

    'Isolation, structure elucidation, and synthesis of a macrophage stimulatory lipopeptide from Mycoplasma fermentans acting at picomolar concentration. ' () 185 J. Exp. Med. : 1951 -1958.

    • Search Google Scholar
  • Frey, J. (2002): Mycoplasmas of Animals. In: Rasin, S. and Herrmann, R. (eds) Molecular Biology and Pathogenicity of Mycoplasmas. Kluwer Academic/Plenum Publishers, New York. pp. 71-90.

    Molecular Biology and Pathogenicity of Mycoplasmas , () 71 -90.

  • Pfützner, H. and Sachse, K. (1996): Mycoplasma bovis as an agent of mastitis, pneumonia, arthritis and genital disorders in cattle. Rev. Sci. Technol. 15, 1477-1494.

    'Mycoplasma bovis as an agent of mastitis, pneumonia, arthritis and genital disorders in cattle. ' () 15 Rev. Sci. Technol. : 1477 -1494.

    • Search Google Scholar
  • Rosengarten, R. and Wise, K. S. (1990): Phenotypic switching in Mycoplasmas: Phase variation of diverse surface lipoproteins. Science 247, 315-318.

    'Phenotypic switching in Mycoplasmas: Phase variation of diverse surface lipoproteins. ' () 247 Science : 315 -318.

    • Search Google Scholar
  • Ross, R. F. (1990): Proc. Mycoplasma Pneumonia Symposia. SmithKline Beecham Animal Health, Ames, Iowa. p. 116.

    , , .

  • Rottem, S. (1980): Membrane lipids of mycoplasmas. Biochim. Biophys. Acta 604, 65-90.

    'Membrane lipids of mycoplasmas. ' () 604 Biochim. Biophys. Acta : 65 -90.

  • Washburn, L. R., Weaver, K. E., Weaver, E. J., Donelan, W. and Al-Sheboul, S. (1998): Molecular characterization of Mycoplasma arthritidis variable surface protein MAA2. Infect. Immun. 66, 2576-2586.

    'Molecular characterization of Mycoplasma arthritidis variable surface protein MAA2. ' () 66 Infect. Immun. : 2576 -2586.

    • Search Google Scholar
  • Zhang, Q., Young, T. F. and Ross, R. F. (1994): Glycolipid receptors for attachment of Mycoplasma hyopneumoniae to porcine respiratory ciliated cells. Infect. Immun. 62, 4367-4373.

    'Glycolipid receptors for attachment of Mycoplasma hyopneumoniae to porcine respiratory ciliated cells. ' () 62 Infect. Immun. : 4367 -4373.

    • Search Google Scholar
  • Galanos, C., Gumenscheimer, M., Muhlradt, P., Jirillo, E. and Freudenberg, M. (2000): MALP-2, a Mycoplasma lipopeptide with classical endotoxic properties: end of an era of LPS monopoly? J. Endotoxin Res. 6, 471-476.

    'MALP-2, a Mycoplasma lipopeptide with classical endotoxic properties: end of an era of LPS monopoly? ' () 6 J. Endotoxin Res. : 471 -476.

    • Search Google Scholar
  • Giron, J. A., Lange, M. and Baseman, J. B. (1996): Adherence, fibronectin binding and induction of cytoskeleton reorganization in cultured human cells by M. penetrans. Infect. Immun. 64, 197-208.

    'Adherence, fibronectin binding and induction of cytoskeleton reorganization in cultured human cells by M. penetrans. ' () 64 Infect. Immun. : 197 -208.

    • Search Google Scholar
  • Smith, P. F., Langworthy, T. A. and Mayberry, W. R. (1976): Distribution and composition of lipopolysaccharides from mycoplasmas. J. Bacteriol. 125, 916-922.

    'Distribution and composition of lipopolysaccharides from mycoplasmas. ' () 125 J. Bacteriol. : 916 -922.

    • Search Google Scholar
  • Stipkovits, L., Molnár, T., Glávits, R., Szabó, I. and Thevasagayam, S. (2004): New opportunities in the control of Mycoplasma pneumonia in pigs [in Hungarian with English abstract]. Magyar Állatorvosok Lapja 126, 325-331.

    'New opportunities in the control of Mycoplasma pneumonia in pigs [in Hungarian with English abstract]. ' () 126 Magyar Állatorvosok Lapja : 325 -331.

    • Search Google Scholar
  • Olson, L. D. and Gilbert, A. A. (1993): Characteristics of Mycoplasma hominis adhesion. J. Bacteriol. 175, 3224-3227.

    'Characteristics of Mycoplasma hominis adhesion. ' () 175 J. Bacteriol. : 3224 -3227.

  • Amoureux, M. C., Hegyi, E., Le, D., Grandics, P., Tong, H. and Szathmary, S. (2004): A new method for removing endotoxin from plasma using hemocompatible affinity chromatography technology, applicable for extracorporeal treatment of septic patients. J. Endotoxin Res. 10, 85-95.

    'A new method for removing endotoxin from plasma using hemocompatible affinity chromatography technology, applicable for extracorporeal treatment of septic patients. ' () 10 J. Endotoxin Res. : 85 -95.

    • Search Google Scholar
  • Takeuchi, O., Kaufmann, A., Grote, K., Kawai, T., Hoshino, K., Morr, M., Muhlradt, P. F. and Akira, S. (2000): Cutting edge: preferentially the R-stereoisomer of the mycoplasmal lipo-lipopeptide macrophage-activating lipopeptide-2 activates immune cells through a Toll-like receptor 2- and MyD88-dependent signaling pathway. J. Immunol. 164, 554-557.

    'Cutting edge: preferentially the R-stereoisomer of the mycoplasmal lipo-lipopeptide macrophage-activating lipopeptide-2 activates immune cells through a Toll-like receptor 2- and MyD88-dependent signaling pathway. ' () 164 J. Immunol. : 554 -557.

    • Search Google Scholar
  • Theiss, P. and Wise, K. S. (1997): Localized frameshift mutation generates selective, highfrequency phase variation of a surface lipoprotein encoded by a Mycoplasma ABC transporter operon. J. Bacteriol. 179, 4013-4022.

    'Localized frameshift mutation generates selective, highfrequency phase variation of a surface lipoprotein encoded by a Mycoplasma ABC transporter operon. ' () 179 J. Bacteriol. : 4013 -4022.

    • Search Google Scholar
  • Nishiguchi, M., Matsumoto, M. and Takao, T. (2001): Mycoplasma fermentans lipoprotein M161 Ag-induced cell activation is mediated by Toll-like receptor 2: Role of N-terminal hydrophobic portion in its multiple functions. J. Immunol. 164, 2610-2616.

    'Mycoplasma fermentans lipoprotein M161 Ag-induced cell activation is mediated by Toll-like receptor 2: Role of N-terminal hydrophobic portion in its multiple functions. ' () 164 J. Immunol. : 2610 -2616.

    • Search Google Scholar
  • Olson, L. D., Shane, S. W., Karpas, A. A., Cunningham, T. M., Probst, P. S. and Barile, M. F. (1991): Monoclonal antibodies to surface antigens of a pathogenic Mycoplasma hominis strain. Infect. Immun. 59, 1683-1689.

    'Monoclonal antibodies to surface antigens of a pathogenic Mycoplasma hominis strain. ' () 59 Infect. Immun. : 1683 -1689.

    • Search Google Scholar
  • Rottem, S. and Barile, M. F. (1993): Beware of mycoplasmas. Tibtech 11, 143-151.

    'Beware of mycoplasmas. ' () 11 Tibtech : 143 -151.

All Time Past Year Past 30 Days
Abstract Views 17 17 1
Full Text Views 6 3 0
PDF Downloads 1 0 0