Acta Veterinaria Hungarica
Volume/Issue:
Volume 54: Issue 3
Rumen fermentation response to a direct-fed xylanase enzyme preparation from Thermomyces lanuginosus in sheep
Authors: V. Jurkovich 1 , J. Kutasi 2 , Hedvig Fébel 3 , J. Reiczigel 4 , E. Brydl 5 , L. Könyves 6 , and P. Rafai 7
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  • 1 Department of Animal Hygiene, Herd Health and Veterinary Ethology, Faculty of Veterinary Science, Szent István University H-1400 Budapest, P.O. Box 2, Hungary
  • 2 Department of Plant Anatomy, Eötvös Lóránd University Budapest, Hungary
  • 3 Research Institute for Animal Breeding and Nutrition Herceghalom, Hungary
  • 4 Department of Biomathematics and Informatics, Faculty of Veterinary Science, Szent István University H-1400 Budapest, P.O. Box 2, Hungary
  • 5 Department of Animal Hygiene, Herd Health and Veterinary Ethology, Faculty of Veterinary Science, Szent István University H-1400 Budapest, P.O. Box 2, Hungary
  • 6 Department of Animal Hygiene, Herd Health and Veterinary Ethology, Faculty of Veterinary Science, Szent István University H-1400 Budapest, P.O. Box 2, Hungary
  • 7 Department of Animal Hygiene, Herd Health and Veterinary Ethology, Faculty of Veterinary Science, Szent István University H-1400 Budapest, P.O. Box 2, Hungary
DOI:
https://doi.org/10.1556/avet.54.2006.3.4
Page Count:
333–342
Publication Date:
01 Sep 2006
Online Publication Date:
15 Aug 2006
Article Category:
Research Article
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A study was conducted to obtain data on the effects of a fungal fibrolytic enzyme preparation (Rumino-zyme, with 250 FXU/g xylanase activities) from Thermomyces lanuginosus on some rumen fermentation parameters in sheep. Ruminal fluid samples were taken just before the morning feeding and then 2 h and 4 h after feeding. Xylanase activity, pH, concentration of ammonia and volatile fatty acids were measured. The enzyme supplementation did not affect the pH but increased the xylanase activity and the total VFA concentration of the rumen fluid. The molar proportion of acetate increased, propionate was not affected and butyrate decreased after enzyme administration. The concentration of ammonia also decreased after supplementation with the enzyme product. It can be concluded that the xylanase enzyme preparation from T. lanuginosus induced favourable changes in the major rumen fermentation parameters in sheep.

  • Bata, Á., Kutasi, J., Jurkovich, V., Fébel, H. and Rafai, P. (2002): Activity and stability of a fungal 1,4-beta-endo-xylanase preparation in the rumen of sheep. J. Anim. Feed Sci. 11, 627-635.

    'Activity and stability of a fungal 1,4-beta-endo-xylanase preparation in the rumen of sheep ' () 11 J. Anim. Feed Sci. : 627 -635.

    • Search Google Scholar

  • Beauchemin, K. A., Rode, L. M. and Sewalt, V. J. (1995): Fibrolytic enzymes increase fibre digestibility and growth rate of steers fed dry forages. Can. J. Anim. Sci. 75, 641-644.

    'Fibrolytic enzymes increase fibre digestibility and growth rate of steers fed dry forages ' () 75 Can. J. Anim. Sci. : 641 -644.

    • Search Google Scholar

  • Beauchemin, K. A., Rode, L. M., Maekawa, M., Morgavi, D. P. and Kampen, R. (2000): Evaluation of a nonstarch polysaccharidase feed enzyme in dairy cows. J. Dairy Sci. 83, 543-553.

    'Evaluation of a nonstarch polysaccharidase feed enzyme in dairy cows ' () 83 J. Dairy Sci. : 543 -553.

    • Search Google Scholar

  • Bennet, N. I., Ryen, J., Biely, P. and Vrasanska, M. (1998): Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Thermomyces lanuginosus ATCC 46882. Carbohydr. Res. 306, 445-455.

    'Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Thermomyces lanuginosus ATCC 46882 ' () 306 Carbohydr. Res. : 445 -455.

    • Search Google Scholar

  • Biely, P., Mislovicova, D. and Toman, R. (1988): Remazol brillant blue xylan: a soluble chromogenic substrates for xylanase. Meth. Enzymol. 160A, 536.

    'Remazol brillant blue xylan: a soluble chromogenic substrates for xylanase ' () 160A Meth. Enzymol. : 536.

    • Search Google Scholar

  • Bowman, G. R., Beauchemin, K. A. and Shelford, J. A. (2002): The proportion of the diet to which fibrolytic enzymes are added affects nutrient digestion by lactating dairy cows. J. Dairy Sci. 85, 3420-3429.

    'The proportion of the diet to which fibrolytic enzymes are added affects nutrient digestion by lactating dairy cows ' () 85 J. Dairy Sci. : 3420 -3429.

    • Search Google Scholar

  • Chaney, A. L. and Marbach, E. P. (1962): Modified reagents for determination of urea and ammonia. Clin. Biochem. 8, 130-132.

    'Modified reagents for determination of urea and ammonia ' () 8 Clin. Biochem. : 130 -132.

    • Search Google Scholar

  • Dhiman, T. R., Zaman, M. S., Gimenez, R. R., Walters, J. L. and Treacher, R. (2002): Performance of dairy cows fed forage treated with fibrolytic enzymes prior to feeding. Anim. Feed Sci. Technol. 101, 115-125.

    'Performance of dairy cows fed forage treated with fibrolytic enzymes prior to feeding ' () 101 Anim. Feed Sci. Technol. : 115 -125.

    • Search Google Scholar

  • Hristov, A. N., McAllister, T. A. and Cheng, K. J. (2000): Intraruminal supplementation with increasing levels of exogenous polysaccharide-degrading enzymes: Effects on nutrient digestion in cattle fed a barley grain diet. J. Anim. Sci. 78, 477-487.

    'Intraruminal supplementation with increasing levels of exogenous polysaccharide-degrading enzymes: Effects on nutrient digestion in cattle fed a barley grain diet ' () 78 J. Anim. Sci. : 477 -487.

    • Search Google Scholar

  • Huhtanen, P. and Khalili, H. (1992): The effect of sucrose supplements on particle associated carboxymethylcellulase (EC 3.2.1.4) and xylanase (EC 3.2.1.8) activities in cattle given grasssilage-based diet. Brit. J. Nutr. 67, 245-255.

    'The effect of sucrose supplements on particle associated carboxymethylcellulase (EC 3.2.1.4) and xylanase (EC 3.2.1.8) activities in cattle given grasssilage-based diet ' () 67 Brit. J. Nutr. : 245 -255.

    • Search Google Scholar

  • Kutasi, J., Bata, Á., Brydl, E., Rafai, P. and Jurkovich, V. (2001): Characterisation and effects of a xylanase enzyme preparation extracted from Thermomyces lanuginosus cultures. Acta Vet. Hung. 49, 175-184.

    'Characterisation and effects of a xylanase enzyme preparation extracted from Thermomyces lanuginosus cultures ' () 49 Acta Vet. Hung. : 175 -184.

    • Search Google Scholar

  • Lee, S. S., Ha, J. K. and Cheng, K. J. (2000): Influence of an anaerobic fungal culture administration on in vivo ruminal fermentation and nutrient digestion. Anim. Feed Sci. Technol. 88, 201-217.

    'Influence of an anaerobic fungal culture administration on in vivo ruminal fermentation and nutrient digestion ' () 88 Anim. Feed Sci. Technol. : 201 -217.

    • Search Google Scholar

  • McAllister, T. A., Oosting, S. J., Popp, J. D., Mir, Z., Hristov, A. N., Treacher, R. J. and Cheng, K. J. (1999): Effect of exogenous enzymes on digestibility of barley silage and growth performance of feedlot cattle. Can. J. Anim. Sci. 79, 353-360.

    'Effect of exogenous enzymes on digestibility of barley silage and growth performance of feedlot cattle ' () 79 Can. J. Anim. Sci. : 353 -360.

    • Search Google Scholar

  • Morgavi, D. P., Beauchemin, K. A., Nsereko, V. L., Rode, L. M., Iwaasa, A. D., Yang, W. Z., McAllister, T. A. and Wang, Y. (2000): Synergy between ruminal fibrolytic enzymes and enzymes from Trichoderma longibrachiatum. J. Dairy Sci. 83, 1310-1321.

    'Synergy between ruminal fibrolytic enzymes and enzymes from Trichoderma longibrachiatum ' () 83 J. Dairy Sci. : 1310 -1321.

    • Search Google Scholar

  • Satter, L. D. and Slyter, L. L. (1974): Effect of ammonia concentration on rumen microbial protein production in vitro. Brit. J. Nutr. 32, 199-208.

    'Effect of ammonia concentration on rumen microbial protein production in vitro ' () 32 Brit. J. Nutr. : 199 -208.

    • Search Google Scholar

  • Schingoethe, D. J., Stegeman, G. A. and Treacher, R. J. (1999): Response of lactating dairy cows to a cellulase and xylanase enzyme mixture applied to forages at the time of feeding. J. Dairy Sci. 82, 996-1003.

    'Response of lactating dairy cows to a cellulase and xylanase enzyme mixture applied to forages at the time of feeding ' () 82 J. Dairy Sci. : 996 -1003.

    • Search Google Scholar

  • Supelco Inc. (1975): G. C. Separation of VFA C2-C5. Bull. 749., Supelco Inc., Bellefonte, PA.

  • Sutton, J. D., Phipps, R. H., Beever, D. E., Humphries, D. J., Hartnell, G. F., Vicini, J. L. and Hard, D. L. (2003): Effect of method of application of a fibrolytic enzyme product on digestive processes and milk production in Holstein-Friesian cows. J. Dairy Sci. 86, 546-556.

    'Effect of method of application of a fibrolytic enzyme product on digestive processes and milk production in Holstein-Friesian cows ' () 86 J. Dairy Sci. : 546 -556.

    • Search Google Scholar

  • Wang, Y., McAllister, T. A., Rode, L. M., Beauchemin, K. A., Morgavi, D. P., Nsereko, V. L., Iwaasa, A. D. and Yang, W. (2001): Effects of an exogenous enzyme preparation on microbial protein synthesis, enzyme activity and attachment to feed in the Rumen Simulation Technique (Rusitec). Brit. J. Nutr. 85, 325-332.

    'Effects of an exogenous enzyme preparation on microbial protein synthesis, enzyme activity and attachment to feed in the Rumen Simulation Technique (Rusitec) ' () 85 Brit. J. Nutr. : 325 -332.

    • Search Google Scholar

  • Yang, W. Z., Beauchemin, K. A. and Rode, L. M. (1999): Effects of an enzyme feed additive on extent of digestion and milk production of lactating dairy cows. J. Dairy Sci. 82, 391-403.

    'Effects of an enzyme feed additive on extent of digestion and milk production of lactating dairy cows ' () 82 J. Dairy Sci. : 391 -403.

    • Search Google Scholar

  • Yang, W. Z., Beauchemin, K. A. and Vedres, D. D. (2002): Effects of pH and fibrolytic enzymes on digestibility, bacterial protein synthesis, and fermentation in continuous culture. Anim. Feed Sci. Technol. 102, 137-150.

    'Effects of pH and fibrolytic enzymes on digestibility, bacterial protein synthesis, and fermentation in continuous culture ' () 102 Anim. Feed Sci. Technol. : 137 -150.

    • Search Google Scholar
Cover Acta Veterinaria Hungarica
Acta Veterinaria Hungarica
Print ISSN:
0236-6290
Online ISSN:
1588-2705
Keywords:
sheep; Xylanase; ruminal fermentation; Thermomyces lanuginosus

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