Application of real-time rt-pcr utilising lux (light upon extension) fluorogenic primer for the rapid detection of avian influenza viruses

I. Kiss; P. Germán; L. Sámi; Márta Antal; T. Farkas; G. Kardos; S. Kecskeméti; Á. Dán; S. Belák

doi:10.1556/avet.54.2006.4.10

Acta Veterinaria Hungarica

Volume/Issue:: Volume 54: Issue 4

Application of real-time rt-pcr utilising lux (light upon extension) fluorogenic primer for the rapid detection of avian influenza viruses

Authors:

I. Kiss

I. Kiss Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

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P. Germán

P. Germán Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

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L. Sámi

L. Sámi Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

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Márta Antal

Márta Antal Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

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T. Farkas

T. Farkas Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

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G. Kardos

G. Kardos Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

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S. Kecskeméti

S. Kecskeméti Central Veterinary Institute, Institute of Debrecen H-4031 Debrecen, Bornemissza u. 3-7, Hungary

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Á. Dán

Á. Dán Central Veterinary Institute Budapest, Hungary

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, and

S. Belák

S. Belák Departments of Virology, National Veterinary Institute and Swedish University of Agricultural Sciences Uppsala, Sweden

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Pages:: 525–533

Online Publication Date:: 18 Oct 2006

Publication Date:: 01 Dec 2006

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/avet.54.2006.4.10

Keywords:: Avian influenza virus; LUX RT-PCR; rapid detection

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A real-time RT-PCR assay utilising light upon extension fluorogenic primer (LUX RT-PCR) was developed for the rapid and efficient detection of avian influenza viruses (AIV). The assay detected each of the AIV isolates tested (16/16) and gave negative results with heterologous pathogens (17/17). The detection limit of the assay proved to be 10^-0.5 EID₅₀/0.2 ml and 10^1.5 EID₅₀/0.2 ml in allantoic fluid of virus-infected embryonated chicken eggs and in spiked chicken faeces samples, respectively. Based on its specificity, sensitivity and relative simplicity, the LUX RT-PCR assay provides a novel, rapid and cost-effective diagnostic tool for avian influenza surveillance and monitoring programs.

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Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language	English
Size	A4
Year of Foundation	1951
Volumes per Year	1
Issues per Year	4
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ISSN	0236-6290 (Print)
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