View More View Less
  • 1 University of Ljubljana Institute for Breeding and Health Care of Wild Animals, Fishes and Bees Gerbičeva 60 1000 Ljubljana Slovenia
  • 2 University of Ljubljana Institute of Physiology, Pharmacology and Toxicology Gerbičeva 60 1000 Ljubljana Slovenia
  • 3 University of Ljubljana Institute for Hygiene and Pathology of Animal Nutrition, Veterinary Faculty Gerbičeva 60 1000 Ljubljana Slovenia
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

A sensitive and reliable method for the determination of trace amounts of abamectin in muscles, kidneys and fat tissue of fallow deer is presented. Abamectin was extracted from the tissues with acetonitrile and the extract was cleaned up on a C8 solid-phase extraction cartridge. Abamectin residue was derivatised with trifluoroacetic acid anhydride and 1-methylimidazole, and determined using reversed- phase high-performance liquid chromatography under isocratic conditions and fluorescence detection. The recoveries of the method were high and consistent, ranging from 78% to 90%. The limit of detection of the method was below 1 μg/kg when analysing muscle, kidney and fat tissue. Matrix-matched calibration was used in order to obtain accurate values and to avoid matrix interference.

  • Ali, M. S., Sun, T., McLeroy, G. E. and Phillippo, E. T. (2000): Simultaneous determination of eprinomectin, moxidectin, abamectin, doramectin, and ivermectin in beef liver by LC with fluorescence detection. J. AOAC Int. 83 , 31–38.

    Phillippo E. T. , 'Simultaneous determination of eprinomectin, moxidectin, abamectin, doramectin, and ivermectin in beef liver by LC with fluorescence detection ' (2000 ) 83 J. AOAC Int. : 31 -38.

    • Search Google Scholar
  • Borges, F. A., Cho, H. S., Santos, E., Oliveira, G. P. and Costa, A. J. (2007): Pharmacokinetics of a new long acting endectocide formulation containing 2.25% ivermectin and 1.25% abamectin in cattle. J. Vet. Pharmacol. Ther. 30 , 62–67.

    Costa A. J. , 'Pharmacokinetics of a new long acting endectocide formulation containing 2.25% ivermectin and 1.25% abamectin in cattle ' (2007 ) 30 J. Vet. Pharmacol. Ther. : 62 -67.

    • Search Google Scholar
  • DeMontigny, P., Shim, J. S. K. and Pivnichny, J. V. (1990): Liquid chromatographic determination of ivermectin in animal plasma with trifluoroacetic anhydride and N-methylimidazole as the derivatization reagent. J. Pharm. Biomed. Anal. 8 , 507–511.

    Pivnichny J. V. , 'Liquid chromatographic determination of ivermectin in animal plasma with trifluoroacetic anhydride and N-methylimidazole as the derivatization reagent ' (1990 ) 8 J. Pharm. Biomed. Anal. : 507 -511.

    • Search Google Scholar
  • European Commission (2002 a ): Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Off. J. Eur. Commun. L 221/8.

  • European Commission (2002 b ): Commission Regulation No. 868/2002 of 24 May 2002 amending Annexes I and II of Council Regulation (EEC) No 2377/90 laying down a Community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. Off. J. Eur. Commun. L 137/6.

  • Diserens, H. and Henzelin, M. (1999): Determination of abamectin residues in fruits and vegetables by high-performance liquid chromatography. J. Chromatogr. A 833 , 13–18.

    Henzelin M. , 'Determination of abamectin residues in fruits and vegetables by high-performance liquid chromatography ' (1999 ) 833 J. Chromatogr. A : 13 -18.

    • Search Google Scholar
  • EMEA (2002): Abamectin, Summary report 3, MRL/813/01-FINAL. European Medicines Agency Veterinary Medicines and Inspections. Available from http://www.emea.europa.eu

  • EMEA (2006): Doramectin, Modification of MRLs, Summary report 6, CMVP/126676. European Medicines Agency Veterinary Medicines and Inspections . Available from http//www.emea.europa.eu

  • Gokbulut, C., Boyacioglu, M. and Karademir, U. (2005): Plasma pharmacokinetics and faecal excretion of ivermectin (Eqvalan® paste) and doramectin (Dectomax® 1%) following oral administration in donkeys. Res. Vet. Sci. 79 , 177–264.

    Karademir U. , 'Plasma pharmacokinetics and faecal excretion of ivermectin (Eqvalan® paste) and doramectin (Dectomax® 1%) following oral administration in donkeys ' (2005 ) 79 Res. Vet. Sci. : 177 -264.

    • Search Google Scholar
  • Hoffman, L. C., Kroucamp, M. and Manley, M. (2007): Meat quality characteristics of springbok ( Antidorcas marsupialis ). 4: Sensory meat evaluation as influenced by age, gender and production region. Meat Sci. 76 , 774–778.

    Manley M. , 'Meat quality characteristics of springbok (Antidorcas marsupialis). 4: Sensory meat evaluation as influenced by age, gender and production region ' (2007 ) 76 Meat Sci. : 774 -778.

    • Search Google Scholar
  • Lankas, G. R. and Gordon, L. R. (1989): Toxicology. In: Campbell, W. C. (ed.) Ivermectin and Abamectin. Springer-Verlag, New York, Berlin, Heidelberg, London, Paris, Tokyo. pp. 89–112.

    Gordon L. R. , '', in Ivermectin and Abamectin , (1989 ) -.

  • Miller, J. N. and Miller, J. C. (2000): The Cochran’s test and the Grubbs’ test. In: Miller, J. N. and Miller, J. C. (eds) Statistics and Chemometrics for Analytical Chemistry. Fourth Edition. Pearson Education Limited, Harlow. pp. 93–98.

    Miller J. C. , '', in Statistics and Chemometrics for Analytical Chemistry , (2000 ) -.

  • Nagata, T., Miyamoto, F., Hasegawa, Y. and Ashizawa, E. (2003): Simultaneous determination of residual antiparasitic lactones in cattle muscle and liver by liquid chromatography with fluorescence detection. J. AOAC Int. 86 , 490–493.

    Ashizawa E. , 'Simultaneous determination of residual antiparasitic lactones in cattle muscle and liver by liquid chromatography with fluorescence detection ' (2003 ) 86 J. AOAC Int. : 490 -493.

    • Search Google Scholar
  • Nordlander, I. and Johnson, H. (1990): Determination of ivermectin residues in swine tissues: an improved cleanup procedure using solid-phase extraction. Food Addit. Contam. 7 , 79–82.

    Johnson H. , 'Determination of ivermectin residues in swine tissues: an improved cleanup procedure using solid-phase extraction ' (1990 ) 7 Food Addit. Contam. : 79 -82.

    • Search Google Scholar
  • Pound, J. M., Miller, J. A. and Oehler, D. D. (2004 a ): Depletion rates of injected and ingested ivermectin from blood serum of penned white-tailed deer, Odocoileus virginianus (Zimmermann) (Artiodactyla: Cervidae). J. Med. Entomol. 41 , 65–68.

    Oehler D. D. , 'Depletion rates of injected and ingested ivermectin from blood serum of penned white-tailed deer, Odocoileus virginianus (Zimmermann) (Artiodactyla: Cervidae) ' (2004 ) 41 J. Med. Entomol. : 65 -68.

    • Search Google Scholar
  • Pound, J. M., Miller, J. A. and Oehler, D. D. (2004 b ): Depletion rate of doramectin from blood serum of penned female white-tailed deer (Artiodactyla: Cervidae). J. Med. Entomol. 41 , 942–945.

    Oehler D. D. , 'Depletion rate of doramectin from blood serum of penned female white-tailed deer (Artiodactyla: Cervidae) ' (2004 ) 41 J. Med. Entomol. : 942 -945.

    • Search Google Scholar
  • Reising, K. P. (1992): Rapid analysis for ivermectin residue in liver and muscle-tissue by liquid chromatography. J. AOAC Int. 75 , 751–753.

    Reising K. P. , 'Rapid analysis for ivermectin residue in liver and muscle-tissue by liquid chromatography ' (1992 ) 75 J. AOAC Int. : 751 -753.

    • Search Google Scholar
  • Stevenson, J. M., Seman, D. L. and Littlejohn, R. P. (1992): Seasonal variation in venison quality of mature, farmed red deer stags in New Zealand. J. Anim. Sci. 70 , 1389–1396.

    Littlejohn R. P. , 'Seasonal variation in venison quality of mature, farmed red deer stags in New Zealand ' (1992 ) 70 J. Anim. Sci. : 1389 -1396.

    • Search Google Scholar
  • Wei, G. Z. and Li, J. S. (2001): Improved method for determination of abamectin and ivermectin in cattle plasma. J. AOAC Int. 84 , 1730–1734.

    Li J. S. , 'Improved method for determination of abamectin and ivermectin in cattle plasma ' (2001 ) 84 J. AOAC Int. : 1730 -1734.

    • Search Google Scholar
  • Wislocki, P. G., Grosso, L. S. and Dybas, R. A. (1989): Environmental aspects of abamectin use in crop protection. In: Campbell, W. C. (eds) Ivermectin and Abamectin. Springer Verlag, New York, Berlin, Heidelberg, London, Paris, Tokyo. pp. 10–14.

    Dybas R. A. , '', in Ivermectin and Abamectin , (1989 ) -.

  • Yong, J. H. and Byeon, W. H. (2005): Alternative production of avermectin components in Streptomyces avermitilis by gene replacement. J. Microbiol. 43 , 277–284.

    Byeon W. H. , 'Alternative production of avermectin components in Streptomyces avermitilis by gene replacement ' (2005 ) 43 J. Microbiol. : 277 -284.

    • Search Google Scholar

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2020 7 0 0
Sep 2020 3 0 0
Oct 2020 0 0 0
Nov 2020 1 0 0
Dec 2020 1 0 0
Jan 2021 2 0 0
Feb 2021 0 0 0