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  • 1 University of Zagreb Department of Chemistry and Biochemistry Heinzelova 55 10000 Zagreb Croatia
  • 2 University of Zagreb Clinic of Internal Medicine Heinzelova 55 10000 Zagreb Croatia
  • 3 University of Zagreb Department of Parasitology, Faculty of Veterinary Medicine Heinzelova 55 10000 Zagreb Croatia
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Changes in coagulation parameters were studied in dogs naturally infected with Babesia canis canis (n = 30), and haemostasis was evaluated and compared to values obtained from healthy dogs (n = 29). To date, there have not been any studies examining the dynamics of thrombin-antithrombin complex formation in cases of canine babesiosis. Coagulation parameters evaluated before (day 0) and on days 1, 2, and 3 after treatment with imidocarb (6 mg/kg inj. s.c.) included the determination of platelet counts, the formation of thrombin-antithrombin complexes (TAT), prothrombin time (PT), activated partial thromboplastin time (APTT) and antithrombin III (AT III) activity. TAT complexes were significantly elevated in animals with babesiosis on days 0 and 2 (mean 49.7 and 87.7 μg/L vs. control, 7.2 μg/L). AT III activity was significantly decreased at all time-points examined. There were no differences in PT. On days 2 and 3 the APTT was significantly shortened in the infected dogs when compared to control animals (means of 21.3 and 19.2 s vs. control, 30.0 s). Our analysis demonstrated that infected dogs had significant thrombocytopenia during the course of the study (mean day 0 − 29 × 10 9 /L, day 1 − 48 × 10 9 /L, day 2 − 47 × 10 9 /L and day 3 − 87 × 10 9 /L, vs. control −259 × 10 9 /L). These data suggest that babesiosis in dogs compromise primary and secondary haemostasis and that induction of disseminated intravascular coagulation (DIC) occurs in canine babesiosis.

  • Bateman, S. W., Mathews, K. A., Abrams-Ogg, A. C., Lumsden, J. H., Johnstone, I. B., Hillers, T. K. and Foster, R. A. (1999): Diagnosis of disseminated intravascular coagulation in dogs admitted to an intensive care unit. J. Am. Vet. Med. Assoc. 215 , 798–804.

    Foster R. A. , 'Diagnosis of disseminated intravascular coagulation in dogs admitted to an intensive care unit ' (1999 ) 215 J. Am. Vet. Med. Assoc. : 798 -804.

    • Search Google Scholar
  • Bick, R. L. (1996): Disseminated intravascular coagulation: Objective clinical and laboratory diagnosis, treatment, and assessment of therapeutic response. Sem. Thromb. Haemost. 22 , 69–88.

    Bick R. L. , 'Disseminated intravascular coagulation: Objective clinical and laboratory diagnosis, treatment, and assessment of therapeutic response ' (1996 ) 22 Sem. Thromb. Haemost. : 69 -88.

    • Search Google Scholar
  • Boozer, A. L. and MacIntire, D. K. (2003): Canine babesiosis. Vet. Clin. N. Amer. Small Anim. Pract. 33 , 885–904.

    MacIntire D. K. , 'Canine babesiosis ' (2003 ) 33 Vet. Clin. N. Amer. Small Anim. Pract. : 885 -904.

    • Search Google Scholar
  • Caccio, S. M., Antunovic, B., Moretti, A., Mangili, V., Marinculić, A., Barić Rafaj, R., Slemenda S. S. and Pieniazek, N. J. (2002): Molecular characterisation of Babesia canis canis and Babesia canis vogeli from naturally infected European dogs. Vet. Parasitol. 106 , 285–292.

    Pieniazek N. J. , 'Molecular characterisation of Babesia canis canis and Babesia canis vogeli from naturally infected European dogs ' (2002 ) 106 Vet. Parasitol. : 285 -292.

    • Search Google Scholar
  • D’Angelo, A., Della Valle, P., Giudici, D. and Viganò D’Angelo, S. (2004): Protein C and coagulation in sepsis. Minerva Anestesiol. 70 , 339–350.

    Viganò D’Angelo S. , 'Protein C and coagulation in sepsis ' (2004 ) 70 Minerva Anestesiol. : 339 -350.

    • Search Google Scholar
  • Esmon, C. T. (2004): Crosstalk between inflammation and thrombosis. Maturitas 47 , 305–314.

    Esmon C. T. , 'Crosstalk between inflammation and thrombosis ' (2004 ) 47 Maturitas : 305 -314.

    • Search Google Scholar
  • Esmon, C. T. (2005): The interaction between inflammation and coagulation. Brit. J. Haematol. 131 , 417–430.

    Esmon C. T. , 'The interaction between inflammation and coagulation ' (2005 ) 131 Brit. J. Haematol. : 417 -430.

    • Search Google Scholar
  • Esmon, C. T., Fukudome, K., Mather, T., Bode, W., Regan, L. M., Stearns-Kurosawa, D. J. and Kurosawa, S. (1999): Inflammation, sepsis, and coagulation. Haematologica 84 , 254–259.

    Kurosawa S. , 'Inflammation, sepsis, and coagulation ' (1999 ) 84 Haematologica : 254 -259.

  • Furlanello, T., Fiorio, F., Caldin, M., Lubas, G. and Solano-Gallego, L. (2005): Clinicopathological findings in naturally occurring cases of babesiosis caused by large form Babesia from dogs of northeastern Italy. Vet. Parasitol. 134 , 77–85.

    Solano-Gallego L. , 'Clinicopathological findings in naturally occurring cases of babesiosis caused by large form Babesia from dogs of northeastern Italy ' (2005 ) 134 Vet. Parasitol. : 77 -85.

    • Search Google Scholar
  • Gentile, N. T., Vaidyula, V. R., Kanamalla, U., DeAngelis, M., Gaughan, J. and Rao, A. K. (2007): Factor VIIa and tissue factor procoagulant activity in diabetes mellitus after acute ischemic stroke: impact of hyperglycemia. Thromb. Haemost. 98 , 1007–1013.

    Rao A. K. , 'Factor VIIa and tissue factor procoagulant activity in diabetes mellitus after acute ischemic stroke: impact of hyperglycemia ' (2007 ) 98 Thromb. Haemost. : 1007 -1013.

    • Search Google Scholar
  • Goodger, B. V., Redebramanis, K. and Wright, I. G. (1987): Procoagulant activity of Babesia bovis -infected erythrocytes. J. Parasitol. 73 , 1052–1060.

    Wright I. G. , 'Procoagulant activity of Babesia bovis-infected erythrocytes ' (1987 ) 73 J. Parasitol. : 1052 -1060.

    • Search Google Scholar
  • Humphries, J. E. (1995): Thrombophilia and complex acquired deficiencies of antithrombin, protein C, and protein S. Semin. Hematol. 32 , 8–16.

    Humphries J. E. , 'Thrombophilia and complex acquired deficiencies of antithrombin, protein C, and protein S ' (1995 ) 32 Semin. Hematol. : 8 -16.

    • Search Google Scholar
  • Jacobson, L. S. (2006): The South African form of severe and complicated canine babesiosis: clinical advances 1994–2004. Vet. Parasitol. 138 , 126–139.

    Jacobson L. S. , 'The South African form of severe and complicated canine babesiosis: clinical advances 1994–2004 ' (2006 ) 138 Vet. Parasitol. : 126 -139.

    • Search Google Scholar
  • Jacobson, L. S. and Clark, I. A. (1994): Supportive treatment of canine babesiosis. J. S. Afr. Vet. Assoc. 66 , 95–105.

    Clark I. A. , 'Supportive treatment of canine babesiosis ' (1994 ) 66 J. S. Afr. Vet. Assoc. : 95 -105.

    • Search Google Scholar
  • Korte, W., Clarke, S. and Lefkowitz, J. B. (2000): Short activated partial thromboplastin times are related to increased thrombin generation and an increased risk for thromboembolism. Am. J. Clin. Pathol. 113 , 123–127.

    Lefkowitz J. B. , 'Short activated partial thromboplastin times are related to increased thrombin generation and an increased risk for thromboembolism ' (2000 ) 113 Am. J. Clin. Pathol. : 123 -127.

    • Search Google Scholar
  • Kume, K., Yamasaki, M., Tashiro, M., Yoshikawa, I. and Otsuki, M. (2007): Activations of coagulation and fibrinolysis secondary to bowel inflammation in patients with ulcerative colitis. Intern. Med. 46 , 1323–1329.

    Otsuki M. , 'Activations of coagulation and fibrinolysis secondary to bowel inflammation in patients with ulcerative colitis ' (2007 ) 46 Intern. Med. : 1323 -1329.

    • Search Google Scholar
  • Mammen, E. F. (2000): Disseminated intravascular coagulation (DIC). Clin. Lab. Sci. 13 , 239–245.

    Mammen E. F. , 'Disseminated intravascular coagulation (DIC) ' (2000 ) 13 Clin. Lab. Sci. : 239 -245.

    • Search Google Scholar
  • Mastrorilli, C., Dondi, F., Agnoli, C., Turba, M. E., Vezzali, E. and Gentilini, F. (2007): Clinicopathologic features and outcome predictors of Leptospira interrogans australis serogroup infection in dogs: A retrospective study of 20 cases (2001–2004). J. Vet. Int. Med. 21 , 3–10.

    Gentilini F. , 'Clinicopathologic features and outcome predictors of Leptospira interrogans australis serogroup infection in dogs: A retrospective study of 20 cases (2001–2004) ' (2007 ) 21 J. Vet. Int. Med. : 3 -10.

    • Search Google Scholar
  • Máthé, Á., Vörös, K., Papp, L. and Reiczigel, J. (2006): Clinical manifestations of canine babesiosis in Hungary (63 cases). Acta Vet. Hung. 54 , 367–385.

    Reiczigel J. , 'Clinical manifestations of canine babesiosis in Hungary (63 cases) ' (2006 ) 54 Acta Vet. Hung. : 367 -385.

    • Search Google Scholar
  • Matijatko, V., Mrljak, V., Kiš, I., Kučer, N., Foršek, J., Živičnjak, T., Romić, Ž., Šimec, Z. and Ceron, J. J. (2007): Evidence of an acute phase response in dogs naturally infected with Babesia canis . Vet. Parasitol. 144 , 242–250.

    Ceron J. J. , 'Evidence of an acute phase response in dogs naturally infected with Babesia canis ' (2007 ) 144 Vet. Parasitol. : 242 -250.

    • Search Google Scholar
  • Matyszko, J. and Tymcio, J. (2008): Thrombin activatable fibrinolysis inhibitor and other hemostatic parameters in patients with essential arterial hypertension. Pol. Arch. Med. Wewn. 118 , 36–41.

    Tymcio J. , 'Thrombin activatable fibrinolysis inhibitor and other hemostatic parameters in patients with essential arterial hypertension ' (2008 ) 118 Pol. Arch. Med. Wewn. : 36 -41.

    • Search Google Scholar
  • Moore, D. J. and Williams, M. C. (1979): Disseminated intravascular coagulation: A complication of Babesia canis infection in the dog. J. S. Afr. Vet. Assoc. 50 , 265–275.

    Williams M. C. , 'Disseminated intravascular coagulation: A complication of Babesia canis infection in the dog ' (1979 ) 50 J. S. Afr. Vet. Assoc. : 265 -275.

    • Search Google Scholar
  • Mrljak, V., Barić Rafaj, R., Sušić, V., Matijatko, V., Kučer, N. and Kiš, I. (2005): Antithrombin III in healthy dogs and in dogs suffering from babesiosis. Vet. Arhiv 75 , 477–486.

    Kiš I. , 'Antithrombin III in healthy dogs and in dogs suffering from babesiosis ' (2005 ) 75 Vet. Arhiv : 477 -486.

    • Search Google Scholar
  • Nel, M., Lobetti, R. G., Keller, N. and Thompson, P. N. (2004): Prognostic value of blood lactate, blood glucose, and hematocrit in canine babesiosis. J. Vet. Intern. Med. 18 , 471–476.

    Thompson P. N. , 'Prognostic value of blood lactate, blood glucose, and hematocrit in canine babesiosis ' (2004 ) 18 J. Vet. Intern. Med. : 471 -476.

    • Search Google Scholar
  • Oglsbee, M. J., Diehl, K., Crawford, R., Kearns, R. and Krakowka, S. (1999): Whole body hyperthermia: effects upon canine immune and hemostatic functions. Vet. Immunol. Immunopathol. 69 , 185–199.

    Krakowka S. , 'Whole body hyperthermia: effects upon canine immune and hemostatic functions ' (1999 ) 69 Vet. Immunol. Immunopathol. : 185 -199.

    • Search Google Scholar
  • Ravanat, C., Freund, M., Dol, F., Cadroy, Y., Roussi, J., Incardona, F., Maffrand, J. P., Boneu, B., Drouet, L. and Legrand, C. (1995): Cross-reactivity of human molecular markers for detection of prethrombotic states in various animal species. Blood Coagul. Fibrinolysis. 6 , 446–455.

    Legrand C. , 'Cross-reactivity of human molecular markers for detection of prethrombotic states in various animal species ' (1995 ) 6 Blood Coagul. Fibrinolysis. : 446 -455.

    • Search Google Scholar
  • Ruiz de Gopegui, R., Penalba, B., Goicoa, A., Espada, Y., Fidalgo, L. E. and Espino, L. (2007): Clinico-pathological findings and coagulation disorders in 45 cases of canine babesiosis in Spain. Vet. J. 174 , 129–132.

    Espino L. , 'Clinico-pathological findings and coagulation disorders in 45 cases of canine babesiosis in Spain ' (2007 ) 174 Vet. J. : 129 -132.

    • Search Google Scholar
  • Schetters, T. P. M. (2005): Vaccination against canine babesiosis. Trends Parasitol. 21 , 179–184.

    Schetters T. P. M. , 'Vaccination against canine babesiosis ' (2005 ) 21 Trends Parasitol. : 179 -184.

    • Search Google Scholar
  • Schetters, T. P. M., Moubri, K., Precigout, E., Kleuskens, J., Scholters, N. C. and Gorenflot, A. (1997): Different Babesia canis isolates, different diseases. Parasitology 115 , 485–493.

    Gorenflot A. , 'Different Babesia canis isolates, different diseases ' (1997 ) 115 Parasitology : 485 -493.

    • Search Google Scholar
  • Tarnow, I., Falk, T., Tidholm, A., Martinussen, T., Jensen, A. L., Olsen, H., Pedersen, H. D. and Kristensen, A. T. (2007): Hemostatic biomarkers in dogs with chronic congestive heart failure. J. Vet. Int. Med. 21 , 451–457.

    Kristensen A. T. , 'Hemostatic biomarkers in dogs with chronic congestive heart failure ' (2007 ) 21 J. Vet. Int. Med. : 451 -457.

    • Search Google Scholar
  • ten Boekel, E., Böck, M., Vrielink, G. J., Liem, R., Hemdriks, H. and de Kieviet, W. (2007): Detection of shortened activated partial thromboplastin times: an evaluation of different commercial reagents. Thromb. Res. 121 , 361–367.

    Kieviet W. , 'Detection of shortened activated partial thromboplastin times: an evaluation of different commercial reagents ' (2007 ) 121 Thromb. Res. : 361 -367.

    • Search Google Scholar
  • ten Boekel, E., de Kieviet, W. and Bartels, P. C. (2003): Subjects with a shortened activated partial thromboplastin time show increased in-hospital mortality associated with elevated Ddimer, C-reactive protein and glucose levels. Scand. J. Clin. Lab. Invest. 63 , 441–448.

    Bartels P. C. , 'Subjects with a shortened activated partial thromboplastin time show increased in-hospital mortality associated with elevated Ddimer, C-reactive protein and glucose levels ' (2003 ) 63 Scand. J. Clin. Lab. Invest. : 441 -448.

    • Search Google Scholar
  • Uilenberg, G., Franssen, F. F., Perié, N. M. and Soanjer, A. A. (1989): Three groups of Babesia canis distinguished and a proposal for nomenclature. Vet. Quart. 11 , 33–40.

    Soanjer A. A. , 'Three groups of Babesia canis distinguished and a proposal for nomenclature ' (1989 ) 11 Vet. Quart. : 33 -40.

    • Search Google Scholar
  • Ulutas, B., Bayramli, G., Ulutas, P. A. and Karagenc, T. (2005): Serum concentration of some acute phase proteins in naturally occurring canine babesiosis: a preliminary study. Vet. Clin. Path. 34 , 144–147.

    Karagenc T. , 'Serum concentration of some acute phase proteins in naturally occurring canine babesiosis: a preliminary study ' (2005 ) 34 Vet. Clin. Path. : 144 -147.

    • Search Google Scholar
  • Warren, B. L., Eid, A., Singer, P., Pillay, S. S., Carl, P., Novak, I., Chalupa, P., Atherstone, A., Pénzes, I., Kübler, A., Knaub, A., Keinecke, H-O., Heinrichs, H., Schindel, F., Juers, M., Bone, R. C. and Opal, S. M. (2001): High-dose antithrombin III in severe sepsis. A randomized controlled trial. J. Am. Med. Assoc. 286 , 1869–1878.

    Opal S. M. , 'High-dose antithrombin III in severe sepsis. A randomized controlled trial ' (2001 ) 286 J. Am. Med. Assoc. : 1869 -1878.

    • Search Google Scholar
  • Wiinberg, B., Jensen, A. L., Johansson, P. I., Rozanski, E., Traholm, M. and Kristensen, A. T. (2008): Thromboelastographic evaluation of hemostatic function in dogs with disseminated intravascular coagulation. J. Vet. Int. Med. 22 , 357–365.

    Kristensen A. T. , 'Thromboelastographic evaluation of hemostatic function in dogs with disseminated intravascular coagulation ' (2008 ) 22 J. Vet. Int. Med. : 357 -365.

    • Search Google Scholar
  • Williamson, L. H. (1991): Antithrombin III: A natural anticoagulant. Comp. Cont. Educ. Vet. 13 , 100–107.

    Williamson L. H. , 'Antithrombin III: A natural anticoagulant ' (1991 ) 13 Comp. Cont. Educ. Vet. : 100 -107.

    • Search Google Scholar
  • Zygner, W., Gójska, O., Rapacka, G., Jaros, D. and Wędrychowitz, H. (2006): Hematological changes during the course of canine babesiosis caused by large Babesia in domestic dogs in Warsaw (Poland). Vet. Parasitol. 145 , 141–151.

    Wędrychowitz H. , 'Hematological changes during the course of canine babesiosis caused by large Babesia in domestic dogs in Warsaw (Poland) ' (2006 ) 145 Vet. Parasitol. : 141 -151.

    • Search Google Scholar

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      Dénes, Béla
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      Fodor, László
      Harrach, Balázs
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      Németh, Tibor
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      Kurt Pfister (Germany)
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      Varga, János
      Štefan, Vilĉek (Slovak Republic)
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      Herbert Weissenböck (Austria)
      Zsarnovszky, Attila

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