Acta Veterinaria Hungarica
Volume/Issue:
Volume 57: Issue 3
Identification of chromosome abnormalities in the horse using a panel of chromosome-specific painting probes generated by microdissection
Authors: Monika Bugno 1 , Ewa Słota 1 , Aldona Pieńkowska-schelling 2 , and Claude Schelling 3
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  • 1 National Research Institute of Animal Production Department of Immuno- and Cytogenetics Krakowska 1 32-083 Balice/Kraków Poland
  • 2 Swiss Federal Institute of Technology Zurich Department of Animal Science Zurich Switzerland
  • 3 Vetsuisse Faculty University of Zurich Department of Veterinary Medicine Zurich Switzerland
DOI:
https://doi.org/10.1556/avet.57.2009.3.3
Page Count:
369–381
Publication Date:
01 Sep 2009
Online Publication Date:
27 Jul 2009
Article Category:
Research Article
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Fluorescent in situ hybridisation (FISH) using a panel of molecular probes for all chromosome pairs obtained by chromosome microdissection of the domestic horse ( Equus caballus ) was used to diagnose karyotype abnormalities in 35 horses (32 mares, 2 stallions and 1 intersex), which were selected for the study due to infertility (23 horses), reduced fertility (10 horses) and developmental anomalies (2 horses). The use of the FISH technique with probes for each horse chromosome pair enabled the diagnosis of many different chromosome aberrations in this population. Among the horses analysed, 21 animals had normal karyotype — 64,XX (19 mares) and 64,XY (2 stallions). Fourteen animals, constituting 40% of the population studied, showed the following chromosome abnormalities: 63,X (1 mare); 63,X/64,XX (6 mares); 63,X/64,XX/65,XXX (3 mares); 63,X/65,XXX (1 mare); 64,XX/65,XX+Xp (1 mare); 63,X/64,XX/65,XX+Xq (1 mare), and 63,X/64,XX/65,XX+delY (1 intersex). When only the mares studied because of complete infertility were taken into consideration, this proportion exceeded 56%. Due to the increased frequency of the above-mentioned aberrations in the mosaic form of two or more lines, it was necessary to analyse a large number (100–300) of metaphase spreads. The use of specific molecular probes obtained by chromosome microdissection made these diagnoses much easier.

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Cover Acta Veterinaria Hungarica
Acta Veterinaria Hungarica
Print ISSN:
0236-6290
Online ISSN:
1588-2705
Keywords:
Horse; painting probes; FISH technique; chromosome aberration

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