Development of Primer-Probe Energy Transfer real-time PCR for the detection and quantification of porcine circovirus type 2

Ádám Bálint; Miklós Tenk; Zoltán Deim; Thomas Rasmussen; Åse Uttenthal; Attila Cságola; Tamás Tuboly; Attila Farsang; Caroline Fossum; Sirje Timmusk; Mikael Berg; Sándor Belák

doi:10.1556/avet.57.2009.3.10

Acta Veterinaria Hungarica

Volume/Issue:: Volume 57: Issue 3

Development of Primer-Probe Energy Transfer real-time PCR for the detection and quantification of porcine circovirus type 2

Authors:

Ádám Bálint

Ádám Bálint

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Miklós Tenk

Miklós Tenk The National Veterinary Institute & The Swedish University of Agricultural Sciences Joint R&D Division, Departments of Virology Ulls väg 2B SE-751 89 Uppsala Sweden

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Zoltán Deim

Zoltán Deim Central Agricultural Office Veterinary Diagnostic Directorate Budapest Hungary

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Thomas Rasmussen

Thomas Rasmussen Technical University of Denmark National Veterinary Institute Lindholm, Kalvehave Denmark

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Åse Uttenthal

Åse Uttenthal Technical University of Denmark National Veterinary Institute Lindholm, Kalvehave Denmark

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Attila Cságola

Attila Cságola Szent István University Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science Budapest Hungary

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Tamás Tuboly

Tamás Tuboly Szent István University Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science Budapest Hungary

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Attila Farsang

Attila Farsang Institute for Veterinary Medicinal Products Department of Virology Budapest Hungary

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Caroline Fossum

Caroline Fossum Swedish University of Agricultural Sciences Department of Biomedical Sciences and Veterinary Public Health Uppsala Sweden

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Sirje Timmusk

Sirje Timmusk Swedish University of Agricultural Sciences Department of Biomedical Sciences and Veterinary Public Health Uppsala Sweden

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Mikael Berg

Mikael Berg

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, and

Sándor Belák

Sándor Belák

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Pages:: 441–452

Online Publication Date:: 27 Jul 2009

Publication Date:: 01 Sep 2009

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/avet.57.2009.3.10

Keywords:: Porcine circovirus type 2; PCV2; diagnosis; real-time PCR; primer-probe energy transfer; PriProET

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A real-time PCR assay, based on Primer-Probe Energy Transfer (PriProET), was developed to improve the detection and quantification of porcine circovirus type 2 (PVC2). PCV2 is recognised as the essential infectious agent in post-weaning multisystemic wasting syndrome (PMWS) and has been associated with other disease syndromes such as porcine dermatitis and nephropathy syndrome (PDNS) and porcine respiratory disease complex (PRDC). Since circoviruses commonly occur in the pig populations and there is a correlation between the severity of the disease and the viral load in the organs and blood, it is important not only to detect PCV2 but also to determine the quantitative aspects of viral load. The PriProET real-time PCR assay described in this study was tested on various virus strains and clinical forms of PMWS in order to investigate any correlation between the clinical signs and viral loads in different organs. The data obtained in this study correlate with those described earlier; namely, the viral load in 1 ml plasma and in 500 ng tissue DNA exceeds 10 ⁷ copies in the case of PMWS. The results indicate that the new assay provides a specific, sensitive and robust tool for the improved detection and quantification of PCV2.

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Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language	English
Size	A4
Year of Foundation	1951
Volumes per Year	1
Issues per Year	4
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ISSN	0236-6290 (Print)
ISSN	1588-2705 (Online)