Tissue tropism of highly pathogenic avian influenza virus subtype H5N1 in naturally infected mute swans ( Cygnus Olor ), domestic geese ( Anser Anser var. domestica ), pekin ducks ( Anas platyrhynchos ) and mulard ducks ( Cairina moschata × anas platyrhynchos )

Levente Szeredi; Ádám Dán; Nimród Pálmai; Krisztina Ursu; Ádám Bálint; Zsófia Szeleczky; Éva Ivanics; Károly Erdélyi; Dóra Rigó; Lajos Tekes; Róbert Glávits

doi:10.1556/avet.58.2010.1.14

Acta Veterinaria Hungarica

Volume/Issue:: Volume 58: Issue 1

Tissue tropism of highly pathogenic avian influenza virus subtype H5N1 in naturally infected mute swans ( Cygnus Olor ), domestic geese ( Anser Anser var. domestica ), pekin ducks ( Anas platyrhynchos ) and mulard ducks ( Cairina moschata × anas platyrhynchos )

Authors:

Levente Szeredi

Levente Szeredi Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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Ádám Dán

Ádám Dán Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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Nimród Pálmai

Nimród Pálmai Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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Krisztina Ursu

Krisztina Ursu Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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Ádám Bálint

Ádám Bálint Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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Zsófia Szeleczky

Zsófia Szeleczky Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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Éva Ivanics

Éva Ivanics Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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Károly Erdélyi

Károly Erdélyi Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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Dóra Rigó

Dóra Rigó Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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Lajos Tekes

Lajos Tekes Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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, and

Róbert Glávits

Róbert Glávits Veterinary Diagnostic Directorate Central Agricultural Office Tábornok u. 2 H-1149 Budapest Hungary

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Pages:: 133–145

Online Publication Date:: 25 Feb 2010

Publication Date:: 01 Mar 2010

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/avet.58.2010.1.14

Keywords:: Highly pathogenic avian influenza virus; H5N1; natural infection; waterfowl; viral antigen; polymerase chain reaction

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The 2006 epidemic due to highly pathogenic avian influenza virus (HPAIV) subtype H5N1 in Hungary caused the most severe losses in waterfowl which were, according to the literature at the time, supposed to be the most resistant to this pathogen. The presence of pathological lesions and the amount of viral antigen were quantified by gross pathology, histopathology and immunohistochemistry (IHC) in the organs of four waterfowl species [mute swans (n = 10), domestic geese (n = 6), mulard ducks (n = 6) and Pekin ducks (n = 5)] collected during the epidemic. H5N1 subtype HPAIV was isolated from all birds examined. Quantitative real-time reverse transcriptase-polymerase chain reaction (qRRT-PCR) was also applied on a subset of samples [domestic geese (n = 3), mulard (n = 4) and Pekin duck (n = 4)] in order to compare its sensitivity with IHC. Viral antigen was detected by IHC in all cases. However, the overall presence of viral antigen in tissue samples was quite variable: virus antigen was present in 56/81 (69%) swan, 22/38 (58%) goose, 28/46 (61%) mulard duck and 5/43 (12%) Pekin duck tissue samples. HPAIV subtype H5N1 was detected by qRRT-PCR in all birds examined, in 19/19 (100%) goose, 7/28 (25%) mulard duck and 12/28 (43%) Pekin duck tissue samples. As compared to qRRTPCR, the IHC was less sensitive in geese and Pekin ducks but more sensitive in mulard ducks. The IHC was consistently positive above 4.31 log10 copies/reaction but it gave very variable results below that level. Neurotropism of the isolated virus strains was demonstrated by finding the largest amount of viral antigen and the highest average RNA load in the brain in all four waterfowl species examined.

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Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

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Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
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