View More View Less
  • 1 University of Veterinary Medicine Komenského 73 041 81 Košice Slovak Republic
  • 2 Slovak Academy of Sciences Institute of Animal Physiology Košice Slovak Republic
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

This study was conducted to investigate the effects of deoxynivalenol (DON) and zearalenone (ZEA) on some biochemical indices of broiler chickens. Twenty-four Ross 308 hybrid broiler chickens of both sexes were fed diets containing maize contaminated with Fusarium mycotoxins. The diets included a control diet (DON 0.60 mg/kg feed; ZEA 0.07 mg/kg feed), an experimental 1 diet (DON 3.4 mg kg −1 feed; ZEA 3.4 mg kg −1 feed), and an experimental 2 diet (DON 8.2 mg kg −1 feed; ZEA 8.3 mg kg −1 feed). Contaminated diets were fed from 14 days of age for 14 days. Blood samples were collected from 4-week-old birds. Chicks fed a diet containing a low level of contaminated maize (experimental 1) had decreased plasma potassium, magnesium, phosphorus, total protein, albumin, triglycerides, free glycerol concentrations and increased cholesterol and calcium levels as well as alkaline phosphatase (ALP) and aspartate aminotransferase (AST) enzyme activities as compared to the control. Feeding a diet contaminated with high levels of mycotoxins (experimental 2) resulted in decreased plasma potassium, magnesium, total protein, albumin, triglycerides, free glycerol concentrations and increased plasma ALP, alanine aminotransferase (ALT) and AST enzyme activities. The effect of mycotoxin-contaminated diets on ALP activity was dose dependent. Chloride concentration was not affected by the diets. It can be concluded that feeding diets contaminated with both levels of Fusarium mycotoxins significantly affected protein, lipid and mineral metabolism as well as AST and ALP enzyme activities in broiler chickens.

  • Abid-Essefi, S., Ouanes, Z., Haassen, W., Baudrimont, I., Creppy, E. and Bacha, H. (2004): Cytotoxicity, inhibition of DNA and protein syntheses and oxidative damage in cultured cells exposed to zearalenone. Toxicol. In Vitro 18 , 467–474.

    Bacha H. , 'Cytotoxicity, inhibition of DNA and protein syntheses and oxidative damage in cultured cells exposed to zearalenone. Toxicol ' (2004 ) 18 In Vitro : 467 -474.

    • Search Google Scholar
  • Bailly, J. D., Le Bars, P., Pietri, A., Benard, G. and Le Bars, J. (1999): Evaluation of a flurodensitometric method for analysis of ergosterol as a fungal marker in compound feeds. J. Food Protect. 62 , 686–690.

    Bars J. , 'Evaluation of a flurodensitometric method for analysis of ergosterol as a fungal marker in compound feeds ' (1999 ) 62 J. Food Protect. : 686 -690.

    • Search Google Scholar
  • Bailly, J. D., Querin, A., Tardieu, D. and Guerre, P. (2005): Production and purification of fumonisins from a highly toxigenic Fusarium verticilloides strain. Rev. Med. Vet. 11 , 547–554.

    Guerre P. , 'Production and purification of fumonisins from a highly toxigenic Fusarium verticilloides strain ' (2005 ) 11 Rev. Med. Vet. : 547 -554.

    • Search Google Scholar
  • Balogh, K., Hausenblasz, J., Weber, M., Erdélyi, M., Fodor, J. and Mézes, M. (2007): Effects of ochratoxin A on some production traits, lipid peroxide and glutathione redox status of weaned piglets. Acta Vet. Hung. 55 , 463–470.

    Mézes M. , 'Effects of ochratoxin A on some production traits, lipid peroxide and glutathione redox status of weaned piglets ' (2007 ) 55 Acta Vet. Hung. : 463 -470.

    • Search Google Scholar
  • Bergsjø, B., Langseth, W., Nafstad, I., Jansen, J. H. and Larsen, H. J. S. (1993): The effects of naturally deoxynivalenol-contaminated oats on the clinical conditions, blood parameters, performance, and carcass composition of growing pigs. Vet. Res. Commun. 17 , 283–294.

    Larsen H. J. S. , 'The effects of naturally deoxynivalenol-contaminated oats on the clinical conditions, blood parameters, performance, and carcass composition of growing pigs ' (1993 ) 17 Vet. Res. Commun. : 283 -294.

    • Search Google Scholar
  • Berthiller, F., Dall’Asta, C., Schumacher, R., Lemmens, M., Adam, G. and Krka, R. (2005): Masked mycotoxins: Determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography-tandem mass spectrophotometry. J. Agric. Food Chem. 53 , 3421–3425.

    Krka R. , 'Masked mycotoxins: Determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography-tandem mass spectrophotometry ' (2005 ) 53 J. Agric. Food Chem. : 3421 -3425.

    • Search Google Scholar
  • Daly, J. A. and Ertingshausen, G. (1972): Direct method for determining inorganic phosphate in serum with ‘CentrifiChem’. Clin. Chem. 18 , 263–265.

    Ertingshausen G. , 'Direct method for determining inorganic phosphate in serum with ‘CentrifiChem’ ' (1972 ) 18 Clin. Chem. : 263 -265.

    • Search Google Scholar
  • Dänicke, S., Valenta, H., Klobasa, F., Döll, S., Ganter, M. and Flachowsky, O. (2004): Effect of graded levels of Fusarium toxin contaminated wheat in diets for fattening pigs on growth performance, nutrient digestibility, deoxynivalenol balance and clinical serum characteristics. Arch. Anim. Nutr. 58 , 1–17.

    Flachowsky O. , 'Effect of graded levels of Fusarium toxin contaminated wheat in diets for fattening pigs on growth performance, nutrient digestibility, deoxynivalenol balance and clinical serum characteristics ' (2004 ) 58 Arch. Anim. Nutr. : 1 -17.

    • Search Google Scholar
  • Doll, S., Dänicke, S., Valenta, H. and Flachowsky, G. (2004): In vitro studies on the evaluation of mycotoxin detoxifying agents for their efficacy on deoxynivalenol and zearalenone. Arch. Anim. Nutr. 58 , 311–324.

    Flachowsky G. , 'In vitro studies on the evaluation of mycotoxin detoxifying agents for their efficacy on deoxynivalenol and zearalenone ' (2004 ) 58 Arch. Anim. Nutr. : 311 -324.

    • Search Google Scholar
  • Doumas, B. T., Bayse, D. D., Carter, R. J., Peters, T. Jr. and Schaffer, R. (1981): A candidate reference method for determination of total protein in serum. I. Development and validation. Clin. Chem. 27 , 1642–1650.

    Schaffer R. , 'A candidate reference method for determination of total protein in serum. I. Development and validation ' (1981 ) 27 Clin. Chem. : 1642 -1650.

    • Search Google Scholar
  • Eriksen, G. S. and Pettersson, H. (2004): Toxicological evaluation of trichothecenes in animal feed. Anim. Feed Sci. Technol. 114 , 205–239.

    Pettersson H. , 'Toxicological evaluation of trichothecenes in animal feed ' (2004 ) 114 Anim. Feed Sci. Technol. : 205 -239.

    • Search Google Scholar
  • Faixová, Z., Faix, Š., Bořutová, R. and Leng, Ľ. (2007): Comparison of two methods to counteract the effects of deoxynivalenol. Folia Vet. 51 , 34–37.

    Leng , 'Comparison of two methods to counteract the effects of deoxynivalenol ' (2007 ) 51 Folia Vet. : 34 -37.

    • Search Google Scholar
  • Gourama, N. and Bullerman, L. B. (1995): Aspergillus flavus and Aspergillus parasiticus : aflatoxigenic fungi of concern in foods and feeds: a review. J. Food Prot. 58 , 1395–1404.

    Bullerman L. B. , 'Aspergillus flavus and Aspergillus parasiticus: aflatoxigenic fungi of concern in foods and feeds: a review ' (1995 ) 58 J. Food Prot. : 1395 -1404.

    • Search Google Scholar
  • Goyarts, T., Grove, N. and Dänicke, S. (2006): Effects of the Fusarium toxin deoxynivalenol from naturally contaminated wheat given subchronically or as one single dose on the in vivo protein synthesis of peripheral blood lymphocytes and plasma protein in the pig. Food Chem. Toxicol. 44 , 1953–1965.

    Dänicke S. , 'Effects of the Fusarium toxin deoxynivalenol from naturally contaminated wheat given subchronically or as one single dose on the in vivo protein synthesis of peripheral blood lymphocytes and plasma protein in the pig ' (2006 ) 44 Food Chem. Toxicol. : 1953 -1965.

    • Search Google Scholar
  • Harvey, R. B., Kubena, L. F., Huff, W. E., Elissalde, M. H. and Phillips, T. D. (1991): Hematological and immunologic toxicity of deoxynivalenol (DON)-contaminated diets to growing chickens. Bull. Environ. Contam. Toxicol. 46 , 410–416.

    Phillips T. D. , 'Hematological and immunologic toxicity of deoxynivalenol (DON)-contaminated diets to growing chickens ' (1991 ) 46 Bull. Environ. Contam. Toxicol. : 410 -416.

    • Search Google Scholar
  • Hunder, G., Schuhann, K., Strugala, G., Gropp, J., Fichtl, B. and Forth, W. (1991): Influence of subtoxic exposure to low dietary deoxynivalenol, a trichothecene mycotoxin, on intestinal absorption of nutrients in mice. Food. Chem. Toxicol. 29 , 809–814.

    Forth W. , 'Influence of subtoxic exposure to low dietary deoxynivalenol, a trichothecene mycotoxin, on intestinal absorption of nutrients in mice ' (1991 ) 29 Food. Chem. Toxicol. : 809 -814.

    • Search Google Scholar
  • Koditschek, I. K. and Umbreit, W. W. (1969): α-Glycerophosphate oxidase in Streptococcus faecium F 24. J. Bacteriol. 98 , 1063–1068.

    Umbreit W. W. , 'α-Glycerophosphate oxidase in Streptococcus faecium F 24 ' (1969 ) 98 J. Bacteriol. : 1063 -1068.

    • Search Google Scholar
  • Kouadio, J. H., Dano, S. D., Moukha, S., Mobio, T. A. and Creppy, E. E. (2007): Effects of combinations of Fusarium mycotoxins on the inhibition of macromolecular synthesis, malondialdehyde levels, DNA methylation and fragmentation, and viability in Caco-2 cells. Toxicon 49 , 306–317.

    Creppy E. E. , 'Effects of combinations of Fusarium mycotoxins on the inhibition of macromolecular synthesis, malondialdehyde levels, DNA methylation and fragmentation, and viability in Caco-2 cells ' (2007 ) 49 Toxicon : 306 -317.

    • Search Google Scholar
  • Kubena, L. F., Edrington, T. S., Harvey, R. B., Buckley, S. A., Phillips, T. D., Rottinghans, G. E. and Casper, H. H. (1997): Individual and combined effects of fumonisin B1 present in Fusarium moniliforme culture material and T-2 toxin or deoxynivalenol on broiler chicks. Poult. Sci. 76 , 1230–1247.

    Casper H. H. , 'Individual and combined effects of fumonisin B1 present in Fusarium moniliforme culture material and T-2 toxin or deoxynivalenol on broiler chicks ' (1997 ) 76 Poult. Sci. : 1230 -1247.

    • Search Google Scholar
  • Kubena, L. F., Swanson, S. P., Harvey, R. B., Fletcher, O. J., Rowe, L. D. and Phillips, T. D. (1985): Effects of feeding deoxynivalenol (vomitoxin)-contaminated wheat to growing chicks. Poult. Sci. 64 , 1649–1655.

    Phillips T. D. , 'Effects of feeding deoxynivalenol (vomitoxin)-contaminated wheat to growing chicks ' (1985 ) 64 Poult. Sci. : 1649 -1655.

    • Search Google Scholar
  • Kuffer, H., Richterich, R., Kraft, R., Peheim, E. and Colombo, J. A. P. (1975): The determination of chloride in plasma and serum (mercury (III) -thiocyanate method) with the Greiner Electronic Selective Analyzer GSA II. Z. Klin. Chem. Klin. Biochem. 5 , 203–211.

    Colombo J. A. P. , 'The determination of chloride in plasma and serum (mercury (III) -thiocyanate method) with the Greiner Electronic Selective Analyzer GSA II ' (1975 ) 5 Z. Klin. Chem. Klin. Biochem. : 203 -211.

    • Search Google Scholar
  • Kuiper-Goodman, T., Scott, P. M. and Watanabe, H. (1987): Risk assessment of mycotoxin zearalenone. Regul. Toxicol. Pharmacol. 7 , 253–306.

    Watanabe H. , 'Risk assessment of mycotoxin zearalenone ' (1987 ) 7 Regul. Toxicol. Pharmacol. : 253 -306.

    • Search Google Scholar
  • Labuda, R., Tancinova, D. and Hudec, K. (2003): Identification and enumeration of Fusarium species in poultry feed mixtures from Slovakia. Ann. Agric. Environ. Med. 10 , 61–66.

    Hudec K. , 'Identification and enumeration of Fusarium species in poultry feed mixtures from Slovakia ' (2003 ) 10 Ann. Agric. Environ. Med. : 61 -66.

    • Search Google Scholar
  • Leung, M. C., Smith, T. K., Karrow, N. A. and Boermans, H. J. (2007): Effects of foodborne Fusarium mycotoxins with and without a polymeric glucomannan mycotoxin adsorbent on food intake and nutrient digestibility, body weight, and physical and clinicopathologic variables of mature dogs. Am. J. Vet. Res. 68 , 1122–1129.

    Boermans H. J. , 'Effects of foodborne Fusarium mycotoxins with and without a polymeric glucomannan mycotoxin adsorbent on food intake and nutrient digestibility, body weight, and physical and clinicopathologic variables of mature dogs ' (2007 ) 68 Am. J. Vet. Res. : 1122 -1129.

    • Search Google Scholar
  • Luongo, D., De Luna, R., Russo, R. and Severino, L. (2008): Effects of four Fusarium toxins (fumonisin B 1 , alpha-zearalenol, nivalenol and deoxynivalenol) on porcine whole blood cellular proliferation. Toxicon 52 , 156–162.

    Severino L. , 'Effects of four Fusarium toxins (fumonisin B1, alpha-zearalenol, nivalenol and deoxynivalenol) on porcine whole blood cellular proliferation ' (2008 ) 52 Toxicon : 156 -162.

    • Search Google Scholar
  • Magan, M., Hope, R., Colleate, A. and Baxter, E. S. (2002): Relationship between growth and mycotoxin production by Fusarium species, biocides and environment. Eur. J. Plant. Pathol. 108 , 685–690.

    Baxter E. S. , 'Relationship between growth and mycotoxin production by Fusarium species, biocides and environment ' (2002 ) 108 Eur. J. Plant. Pathol. : 685 -690.

    • Search Google Scholar
  • Malekinejad, H., Maas-Bakker, R. and Fink-Gremmel, S. (2006): Species differences in the hepatic biotransformation of zearalenone. Vet. J. 172 , 96–102.

    Fink-Gremmel S. , 'Species differences in the hepatic biotransformation of zearalenone ' (2006 ) 172 Vet. J. : 96 -102.

    • Search Google Scholar
  • Mann, C. K. and Yoe, J. H. (1957): Spectrophotometric determination of magnesium with sodium 1-azo-2-hydroxy-3-(2,4-dimethylcarboxanilido naphthalene-1′-(2-hydroxybenzene). Anal. Clin. Acta 16 , 155–160.

    Yoe J. H. , 'Spectrophotometric determination of magnesium with sodium 1-azo-2-hydroxy-3-(2,4-dimethylcarboxanilido naphthalene-1′-(2-hydroxybenzene) ' (1957 ) 16 Anal. Clin. Acta : 155 -160.

    • Search Google Scholar
  • Minervini, F., Giannocaro, A., Cavallini, A. and Visconti, A. (2005): Investigations on cellular proliferation induced by zearalenone and its derivates in relation to the estrogenic parameters. Toxicol. Lett. 159 , 272–283.

    Visconti A. , 'Investigations on cellular proliferation induced by zearalenone and its derivates in relation to the estrogenic parameters ' (2005 ) 159 Toxicol. Lett. : 272 -283.

    • Search Google Scholar
  • Pál, L., Dublecz, K., Weber, M., Balogh, K., Erdélyi, M., Szigeti, G. and Mézes, M. (2009): Effect of combined treatment with aflatoxin B 1 and T-2 toxin and metabolites on some production traits and lipid peroxide status parameters of broiler chickens. Acta Vet. Hung. 57 , 75–84.

    Mézes M. , 'Effect of combined treatment with aflatoxin B1 and T-2 toxin and metabolites on some production traits and lipid peroxide status parameters of broiler chickens ' (2009 ) 57 Acta Vet. Hung. : 75 -84.

    • Search Google Scholar
  • Pestka, J. J., Zhou, H. R., Moon, Y. and Chung, Y. J. (2004): Cellular and molecular mechanisms for immune modulation by deoxynivalenol and other trichothecenes: unraveling a paradox. Toxicol. Lett. 153 , 61–73.

    Chung Y. J. , 'Cellular and molecular mechanisms for immune modulation by deoxynivalenol and other trichothecenes: unraveling a paradox ' (2004 ) 153 Toxicol. Lett. : 61 -73.

    • Search Google Scholar
  • Ray Sarkar, B. C. and Chauhan, U. S. P. (1967): A new method for the determination of microquantities of calcium in biological material. Anal. Biochem. 20 , 155–166.

    Chauhan U. S. P. , 'A new method for the determination of microquantities of calcium in biological material ' (1967 ) 20 Anal. Biochem. : 155 -166.

    • Search Google Scholar
  • Raymond, S. L., Smith, T. K. and Swamy, H. V. L. N. (2003): Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on feed intake, serum chemistry, and hematology of horses, and the efficacy of a polymeric glucomannan-mycotoxin adsorbent. J. Anim. Sci. 81 , 2123–2130.

    Swamy H. V. L. N. , 'Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on feed intake, serum chemistry, and hematology of horses, and the efficacy of a polymeric glucomannan-mycotoxin adsorbent ' (2003 ) 81 J. Anim. Sci. : 2123 -2130.

    • Search Google Scholar
  • Rotter, B. A., Prelusky, D. B. and Pestka, J. J. (1996): Toxicology of deoxynivalenol (vomitoxin). J. Toxicol. Environ. Health 48 , 1–34.

    Pestka J. J. , 'Toxicology of deoxynivalenol (vomitoxin) ' (1996 ) 48 J. Toxicol. Environ. Health : 1 -34.

    • Search Google Scholar
  • Smith, T. K., McMillan, E. G. and Castillo, J. B. (1997): Effect of feeding blends of Fusarium mycotoxin-contaminated grains containing deoxynivalenol and fusaric acid on growth and feed consumption of immature swine. J. Anim. Sci. 75 , 2184–2191.

    Castillo J. B. , 'Effect of feeding blends of Fusarium mycotoxin-contaminated grains containing deoxynivalenol and fusaric acid on growth and feed consumption of immature swine ' (1997 ) 75 J. Anim. Sci. : 2184 -2191.

    • Search Google Scholar
  • Szkudelska, K., Szkudelski, T. and Nogowski, L. (2002): Short-term deoxynivalenol treatment induces metabolic disturbances in the rat. Toxicol. Lett. 136 , 25–31.

    Nogowski L. , 'Short-term deoxynivalenol treatment induces metabolic disturbances in the rat ' (2002 ) 136 Toxicol. Lett. : 25 -31.

    • Search Google Scholar
  • Wu, X., Kohut, M., Cunnick, J., Bailley, T. and Hendrich, S. (2009): Deoxynivalenol suppresses circulating and splenic leukocyte subpopulations in BALB/c mice: dose response, time course and sex differences. Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess. 7 , 1070–1080.

    Hendrich S. , 'Deoxynivalenol suppresses circulating and splenic leukocyte subpopulations in BALB/c mice: dose response, time course and sex differences ' (2009 ) 7 Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess. : 1070 -1080.

    • Search Google Scholar
  • Yegani, M., Smith, T. K., Leeson, S. and Boermans, H. J. (2006): Effects of feeding grains naturally contaminated with Fusarium mycotoxins on performance and metabolism of broiler breeders. Poult. Sci. 85 , 1541–1549.

    Boermans H. J. , 'Effects of feeding grains naturally contaminated with Fusarium mycotoxins on performance and metabolism of broiler breeders ' (2006 ) 85 Poult. Sci. : 1541 -1549.

    • Search Google Scholar

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Sep 2020 5 0 0
Oct 2020 8 0 0
Nov 2020 3 0 0
Dec 2020 1 0 0
Jan 2021 1 0 0
Feb 2021 10 0 0
Mar 2021 0 0 0