Detection of Vibrio parahaemolyticus in mediterranean mussels (Mytilus galloprovincialis) in Slovenia

Urška Henigman; Majda Biasizzo; Stanka Vadnjal; Andrej Kirbiš; Ivan Toplak; Darja Barlič-Maganja

doi:10.1556/avet.2011.010

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Acta Veterinaria Hungarica

Volume/Issue:: Volume 59: Issue 2

Detection of Vibrio parahaemolyticus in mediterranean mussels (Mytilus galloprovincialis) in Slovenia

Authors:

Urška Henigman

Urška Henigman University of Ljubljana Institute for Food Hygiene, Veterinary Faculty Gerbičeva 60 1000 Ljubljana Slovenia

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Majda Biasizzo

Majda Biasizzo University of Ljubljana Institute for Food Hygiene, Veterinary Faculty Gerbičeva 60 1000 Ljubljana Slovenia

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Stanka Vadnjal

Stanka Vadnjal University of Ljubljana Institute for Food Hygiene, Veterinary Faculty Gerbičeva 60 1000 Ljubljana Slovenia

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Andrej Kirbiš

Andrej Kirbiš University of Ljubljana Institute for Food Hygiene, Veterinary Faculty Gerbičeva 60 1000 Ljubljana Slovenia

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Ivan Toplak

Ivan Toplak University of Ljubljana Institute for Microbiology and Parasitology, Virology Unit Ljubljana Slovenia

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Darja Barlič-Maganja

Darja Barlič-Maganja

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Pages:: 155–164

Online Publication Date:: 05 Apr 2011

Publication Date:: 01 Jun 2011

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/avet.2011.010

Keywords:: Mussels; PCR

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The aim of this study was to determine the prevalence of Vibrio parahaemolyticus in shellfish samples harvested along the Slovenian coast. Shellfish samples of Mediterranean mussels (Mytilus galloprovincialis) were collected along the Slovenian coast at four locations (Seča, Piran, Strunjan and Debeli Rtič) between 2006 and 2008. Samples were examined and analysed for the presence of V. parahaemolyticus by conventional and molecular methods. The presence of Vibrio in the samples was examined by conventional methods on plate grown bacterial cells before and after enrichment in alkaline saline peptone water (ASPW). PCR methods were used for the detection of V. parahaemolyticus-specific toxR and tlh genes and of the virulence-associated tdh and trh genes. Out of 168 samples examined, 24 were positive for toxR and tlh genes by PCR from enrichment broth. Five out of 62 (8.1%), 4 out of 32 (12.5%) and 15 out of 74 (20.2%) samples were positive in 2006, 2007 and 2008, respectively. Colonies of V. parahaemolyticus were isolated from only one sample positive for V. parahaemolyticus by PCR.

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Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language	English
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