Acta Veterinaria Hungarica
Volume/Issue:
Volume 59: Issue 2
Effect of recombinant interleukin-10 on some haematological and biochemical parameters in a rat endotoxaemic model
Authors: Ramazan Çöl 1 and Zafer Durgun 1
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  • 1 University of Selçuk Department of Physiology, Faculty of Veterinary Medicine Campus 42031 Selçuklu, Konya Turkey
DOI:
https://doi.org/10.1556/avet.2011.009
Page Count:
237–245
Publication Date:
01 Jun 2011
Online Publication Date:
05 Apr 2011
Article Category:
Research Article
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Recombinant interleukin-10 (rIL10) has been found to suppress the synthesis of tumour necrosis factor (TNF), interleukin-1 (IL-1), interleukin-6 (IL-6) and tissue factor and to improve survival from experimental sepsis. The aim of this study was to evaluate the protective effect of rIL-10 on lipopolysaccharide-(LPS-) induced haematological and biochemical disturbances in rats. In the present study, 40 rats were used and divided equally into four groups. Group 1 (control group, C) was treated with 0.9% saline. Group 2: LPS was injected intravenously (1.6 mg/100 g), Group 3 received rIL10 treatment (125 μg/kg) 2 min before 0.9% saline injection, Group 4 received rIL10 treatment 2 min before endotoxin treatment. When compared with the controls, platelet count, leukocyte count (with a marked neutrophilia and lymphopenia) and fibrinogen were decreased, while activated partial thromboplastin time (APTT) and prothrombin time (PT) were prolonged in the endotoxaemic rats. In addition, LPS caused statistically significant increases in plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities as well as creatinine, cholesterol and triglyceride concentrations, while it caused a statistically significant decrease in glucose, total protein and albumin levels as compared to the control group. On the other hand, rIL10 significantly suppressed disturbances in the haematological and biochemical parameters associated with endotoxaemia. As a result, rIL10 may be efficacious in preventing haematological disorders, tissue damage and changes in lipid, protein and carbohydrate metabolism in endotoxaemia.

  • Agarwal, R. and Gupta, D. (2005): Anticoagulation in sepsis: is low-dose heparin as effective as activated protein C. Intensive Care Med. 31, 1297–1298.

    Gupta D. , 'Anticoagulation in sepsis: is low-dose heparin as effective as activated protein C ' (2005 ) 31 Intensive Care Med. : 1297 -1298.

    • Search Google Scholar

  • Al-Dughaym, A. M. (2004): Some endotoxin-induced clinical and biochemical changes in plasma of camels (Camelus dromedarius). Vet. Res. Commun. 28, 711–718.

    Al-Dughaym A. M. , 'Some endotoxin-induced clinical and biochemical changes in plasma of camels (Camelus dromedarius) ' (2004 ) 28 Vet. Res. Commun. : 711 -718.

    • Search Google Scholar

  • Asakura, H., Asamura, R., Ontachi, Y., Hayashi, T., Yamazaki, M., Morishita, E., Miyamoto, K. I. and Nakao, S. (2005): Selective inducible nitric oxide synthase inhibition attenuates organ dysfunction and elevated endothelin levels in LPS-induced DIC model rats. J. Thromb. Haemost. 3, 1050–1055.

    Nakao S. , 'Selective inducible nitric oxide synthase inhibition attenuates organ dysfunction and elevated endothelin levels in LPS-induced DIC model rats ' (2005 ) 3 J. Thromb. Haemost. : 1050 -1055.

    • Search Google Scholar

  • Barsness, K. A., Bensard, D. D., Partrick, D. A., Calkins, C. M., Hendrickson, R. J. and McIntyre, R. C. (2004): Endotoxin induces an exaggerated interleukin-10 response in peritoneal macrophages of children compared with adults. J. Pediatr. Surg. 39, 912–915.

    McIntyre R. C. , 'Endotoxin induces an exaggerated interleukin-10 response in peritoneal macrophages of children compared with adults ' (2004 ) 39 J. Pediatr. Surg. : 912 -915.

    • Search Google Scholar

  • Bussolati, B., Mariano, F., Montrucchio, G., Piccoli, G. and Camussi, G. (1997): Modulatory effect of interleukin-10 on the production of platelet-activating factor and superoxide anions by human leucocytes. Immunology 90, 440–447.

    Camussi G. , 'Modulatory effect of interleukin-10 on the production of platelet-activating factor and superoxide anions by human leucocytes ' (1997 ) 90 Immunology : 440 -447.

    • Search Google Scholar

  • Chen, C. P., Yokozawa, T. and Kitani, K. (1999): Beneficial effects of Sanguisorbae radix in renal dysfunction caused by endotoxin in vivo. Biol. Pharm. Bull. 22, 1327–1330.

    Kitani K. , 'Beneficial effects of Sanguisorbae radix in renal dysfunction caused by endotoxin in vivo ' (1999 ) 22 Biol. Pharm. Bull. : 1327 -1330.

    • Search Google Scholar

  • Çöl, R., Keskin, E. and Atalay, B. (2005): Effect of pentoxifylline on endotoxin-induced haemostatic disturbances in rabbits. Acta Vet. Hung. 53, 325–335.

    Atalay B. , 'Effect of pentoxifylline on endotoxin-induced haemostatic disturbances in rabbits ' (2005 ) 53 Acta Vet. Hung. : 325 -335.

    • Search Google Scholar

  • Dhuley, J. N. and Naik, S. R. (1998): Effect of rhinax on bacterial lipopolysaccharide induced endotoxemia in rats. Indian J. Exp. Biol. 36, 315–317.

    Naik S. R. , 'Effect of rhinax on bacterial lipopolysaccharide induced endotoxemia in rats ' (1998 ) 36 Indian J. Exp. Biol. : 315 -317.

    • Search Google Scholar

  • Fraunberg, P., Schaefer, S., Werdan, K., Walli, A. K. and Seidel, D. (1999): Reduction of circulating cholesterol and apolipoprotein levels during sepsis. Clin. Chem. Lab. Med. 37, 357–362.

    Seidel D. , 'Reduction of circulating cholesterol and apolipoprotein levels during sepsis ' (1999 ) 37 Clin. Chem. Lab. Med. : 357 -362.

    • Search Google Scholar

  • Gaviria, J. M., Dale, D. C. and Root, R. K. (1998): Neutrophils: Function and role in sepsis syndrome. Sepsis 2, 107–117.

    Root R. K. , 'Neutrophils: Function and role in sepsis syndrome ' (1998 ) 2 Sepsis : 107 -117.

    • Search Google Scholar

  • Hoffmann, J. N., Vollmar, B., Römisch, J., Inthorn, D., Schildberg, F. W. and Menger, M. D. (2002): Antithrombin effects on endotoxin-induced microcirculatory disorders are mediated mainly by its interaction with microvascular endothelium. Crit. Care Med. 30, 218–225.

    Menger M. D. , 'Antithrombin effects on endotoxin-induced microcirculatory disorders are mediated mainly by its interaction with microvascular endothelium ' (2002 ) 30 Crit. Care Med. : 218 -225.

    • Search Google Scholar

  • Hopper, K. and Bateman, S. (2005): An updated view of hemostasis: mechanisms of hemostatic dysfunction associated with sepsis. J. Vet. Emerg. Crit. Care 15, 83–91.

    Bateman S. , 'An updated view of hemostasis: mechanisms of hemostatic dysfunction associated with sepsis ' (2005 ) 15 J. Vet. Emerg. Crit. Care : 83 -91.

    • Search Google Scholar

  • Howard, M., Muchamuel, T., Andrade, S. and Menon, S. (1993): Interleukin 10 protects mice from lethal endotoxemia. J. Exp. Med. 177, 1205–1208.

    Menon S. , 'Interleukin 10 protects mice from lethal endotoxemia ' (1993 ) 177 J. Exp. Med. : 1205 -1208.

    • Search Google Scholar

  • Hryszko, T., Inaba, K., Ihara, H., Suzuki, Y., Mogami, H. and Urano, T. (2006): Nafamostat attenuated the impairment of fibrinolysis in animal sepsis model by suppressing the increase of plasminogen activator inhibitor type 1. J. Trauma 60, 859–864.

    Urano T. , 'Nafamostat attenuated the impairment of fibrinolysis in animal sepsis model by suppressing the increase of plasminogen activator inhibitor type 1 ' (2006 ) 60 J. Trauma : 859 -864.

    • Search Google Scholar

  • Iba, T., Nakarai, E., Takayama, T., Nakajima, K., Sasaoka, T. and Ohno, Y. (2009): Combination effect of antithrombin and recombinant human soluble thrombomodulin in a lipopolysaccharide induced rat sepsis model. Crit. Care 13, R203.

    Ohno Y. , 'Combination effect of antithrombin and recombinant human soluble thrombomodulin in a lipopolysaccharide induced rat sepsis model ' (2009 ) 13 Crit. Care : R203 -.

    • Search Google Scholar

  • Izutani, W., Fujita, M., Nishizawa, K. and Koga, J. (2000): Urinary protein C inhibitor as a therapeutic agent to disseminated intravascular coagulation (DIC): A comparison with low molecular weight heparin in rats with lipopolysaccharide-induced DIC. Biol. Pharm. Bull. 23, 1046–1050.

    Koga J. , 'Urinary protein C inhibitor as a therapeutic agent to disseminated intravascular coagulation (DIC): A comparison with low molecular weight heparin in rats with lipopolysaccharide-induced DIC ' (2000 ) 23 Biol. Pharm. Bull. : 1046 -1050.

    • Search Google Scholar

  • Jeschke, M. G., Rensing, H., Klein, D., Schubert, T., Mautes, A. E., Bolder, U. and Croner, R. S. (2005): Insulin prevents liver damage and preserves liver function in lipopolysaccharide-induced endotoxaemic rats. J. Hepatol. 42, 870–879.

    Croner R. S. , 'Insulin prevents liver damage and preserves liver function in lipopolysaccharide-induced endotoxaemic rats ' (2005 ) 42 J. Hepatol. : 870 -879.

    • Search Google Scholar

  • Lanza-Jacoby, S., Phetteplace, H., Sedkova, N. and Knee, G. (1998): Sequential alterations in tissue lipoprotein lipase, triglyceride secretion rates, and serum tumor necrosis factor alpha during Escherichia coli bacteremic sepsis in relation to the development of hypertriglyceridemia. Shock 9, 46–51

    Knee G. , 'Sequential alterations in tissue lipoprotein lipase, triglyceride secretion rates, and serum tumor necrosis factor alpha during Escherichia coli bacteremic sepsis in relation to the development of hypertriglyceridemia ' (1998 ) 9 Shock : 46 -51.

    • Search Google Scholar

  • Lauw, F. N., Pajkrt, D., Hack, C. E., Kurimoto, M., Van Deventer, S. J. H. and van der Poll, T. (2000): Proinflammatory effects of IL-10 during human endotoxemia. J. Immunol. 165, 2783–2789.

    Poll T. , 'Proinflammatory effects of IL-10 during human endotoxemia ' (2000 ) 165 J. Immunol. : 2783 -2789.

    • Search Google Scholar

  • Levi, M., Van der Poll, T., ten Cate, H. and van Deventer, S. J. H. (1997): The cytokine-mediated imbalance between coagulant and anticoagulant mechanism in sepsis and endotoxaemia. Eur. J. Clin. Invest. 27, 3–9.

    Deventer S. J. H. , 'The cytokine-mediated imbalance between coagulant and anticoagulant mechanism in sepsis and endotoxaemia ' (1997 ) 27 Eur. J. Clin. Invest. : 3 -9.

    • Search Google Scholar

  • Møller, S., Laigaard, F., Olgaard, K. and Hemmingsen, C. (2007): Effect of 1,25-dihydroxyvitamin D3 in experimental sepsis. Int. J. Med. Sci. 4, 190–195.

    Hemmingsen C. , 'Effect of 1,25-dihydroxyvitamin D3 in experimental sepsis ' (2007 ) 4 Int. J. Med. Sci. : 190 -195.

    • Search Google Scholar

  • Nagaki, M., Tanaka, M., Sugiyama, A., Ohnishi, H. and Moriwaki, H. (1999): Interleukin-10 inhibits hepatic injury and tumor necrosis factor-a and interferon-γ mRNA expression induced by staphylococcal enterotoxin B or lipopolysaccharide in galactosamine-sensitized mice. J. Hepatol. 31, 815–824.

    Moriwaki H. , 'Interleukin-10 inhibits hepatic injury and tumor necrosis factor-a and interferon-γ mRNA expression induced by staphylococcal enterotoxin B or lipopolysaccharide in galactosamine-sensitized mice ' (1999 ) 31 J. Hepatol. : 815 -824.

    • Search Google Scholar

  • Nussler, A. K. and Billiar, T. R. (1993): Inflammation, immunoregulation, and inducible nitric oxide synthase. J. Leukoc. Biol. 54, 171–178.

    Billiar T. R. , 'Inflammation, immunoregulation, and inducible nitric oxide synthase ' (1993 ) 54 J. Leukoc. Biol. : 171 -178.

    • Search Google Scholar

  • Oikawa, M. and Yamaoka, S. (2003): Clinical, hematological, and biochemical analysis of experimental endotoxemia in Thoroughbred horses. J. Equine Sci. 14, 5–12.

    Yamaoka S. , 'Clinical, hematological, and biochemical analysis of experimental endotoxemia in Thoroughbred horses ' (2003 ) 14 J. Equine Sci. : 5 -12.

    • Search Google Scholar

  • Pajkrt, B. D., van der Poll, T., Levi, M., Cutler, D. L., Affrime, M. B., van den Ende, A., ten Cate, J. W. and van Deventer, S. J. H. (1997): Interleukin-10 inhibits activation of coagulation and fibrinolysis during human endotoxemia. Blood 89, 2701–2705.

    Deventer S. J. H. , 'Interleukin-10 inhibits activation of coagulation and fibrinolysis during human endotoxemia ' (1997 ) 89 Blood : 2701 -2705.

    • Search Google Scholar

  • Ramani, M., Ollivier, V., Khechai, F., Vu, T., Ternisien, C., Bridey, F. and de Prost, D. (1993): Interleukin-10 inhibits endotoxin-induced tissue factor mRNA production by human monocytes. FEBS Lett. 334, 114–116.

    Prost D. , 'Interleukin-10 inhibits endotoxin-induced tissue factor mRNA production by human monocytes ' (1993 ) 334 FEBS Lett : 114 -116.

    • Search Google Scholar

  • Scherer, R. U., Giebler, R. M., Schmidt, U., Paar, D., Wüst, T., Spangenberg, P., Militzer, K., Hirche, H. and Kox, W. J. (1995): Short-time rabbit model of endotoxin-induced hypercoagulability. Lab. Anim. Sci. 45, 538–546.

    Kox W. J. , 'Short-time rabbit model of endotoxin-induced hypercoagulability ' (1995 ) 45 Lab. Anim. Sci. : 538 -546.

    • Search Google Scholar

  • Semrad, S. D. (1993): Comparison of flunixin, prednisolone, dimethyl sulfoxide, and a lazaroid (U74389F) for treating endotoxemic neonatal calves. Am. J. Vet. Res. 54, 1517–1522.

    Semrad S. D. , 'Comparison of flunixin, prednisolone, dimethyl sulfoxide, and a lazaroid (U74389F) for treating endotoxemic neonatal calves ' (1993 ) 54 Am. J. Vet. Res. : 1517 -1522.

    • Search Google Scholar

  • Southorn, B. G. and Thompson, J. R. (1986): Time course changes in blood metabolites during endotoxin fever in sheep. Can. J. Vet. Res. 50, 374–379.

    Thompson J. R. , 'Time course changes in blood metabolites during endotoxin fever in sheep ' (1986 ) 50 Can. J. Vet. Res. : 374 -379.

    • Search Google Scholar

  • Tinsley, J. H., Chiasson, V. L., South, S., Mahajan, A. and Mitchell, B. M. (2009): Immunosuppression improves blood pressure and endothelial function in a rat model of pregnancyinduced hypertension. Am. J. Hypertens. 22, 1107–1114.

    Mitchell B. M. , 'Immunosuppression improves blood pressure and endothelial function in a rat model of pregnancyinduced hypertension ' (2009 ) 22 Am. J. Hypertens. : 1107 -1114.

    • Search Google Scholar

  • van Devender, S. J. H., Buller, H. R., ten Cate, J. W., Aareden, L. A., Hack, C. E. and Sturk, A. (1990): Experimental endotoxemia in humans; analysis of cytokine release and coagulation, fibrinolytic, and complement pathways. Blood 76, 2520–2526.

    Sturk A. , 'Experimental endotoxemia in humans; analysis of cytokine release and coagulation, fibrinolytic, and complement pathways ' (1990 ) 76 Blood : 2520 -2526.

    • Search Google Scholar

  • Wang, Z., Su, F., Rogiers, P. and Vincent, J. (2007): Beneficial effects of recombinant human activated protein C in a ewe model of septic shock. Crit. Care Med. 35, 2594–2600.

    Vincent J. , 'Beneficial effects of recombinant human activated protein C in a ewe model of septic shock ' (2007 ) 35 Crit. Care Med. : 2594 -2600.

    • Search Google Scholar

  • Yang, H., Li, Q. W., Han, Z. S., Hu, J. H., Li, W. Y. and Liu, Z. B. (2009): Recombinant human antithrombin expressed in the milk of non-transgenic goats exhibits high efficiency on rat DIC model. J. Thromb. Thrombolysis 28, 449–457.

    Liu Z. B. , 'Recombinant human antithrombin expressed in the milk of non-transgenic goats exhibits high efficiency on rat DIC model ' (2009 ) 28 J. Thromb. Thrombolysis : 449 -457.

    • Search Google Scholar

  • Yazar, E., Çöl, R., Uney, K., Atalay, B., Elmas, M. and Tras, B. (2004): Effect of pentoxifylline on biochemical parameters in endotoxemic New Zealand White rabbits. Bull. Vet. Inst. PuŁawy 48, 297–299.

    Tras B. , 'Effect of pentoxifylline on biochemical parameters in endotoxemic New Zealand White rabbits ' (2004 ) 48 Bull. Vet. Inst. PuŁawy : 297 -299.

    • Search Google Scholar
Cover Acta Veterinaria Hungarica
Acta Veterinaria Hungarica
Print ISSN:
0236-6290
Online ISSN:
1588-2705
Keywords:
Recombinant interleukin-10; haematological and biochemical parameters; endotoxin; rat

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