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  • 1 Szentes University Teaching Hospital Department of Traumatology and Bone & Joint Reconstructive Surgery Szentes Hungary
  • 2 Erzsébet Hospital Department of Traumatology Sopron Hungary
  • 3 Semmelweis University Department of Orthopaedics Budapest Hungary
  • 4 Szent István University Department and Clinic of Surgery and Ophthalmology, Faculty of Veterinary Science Budapest Hungary
  • 5 Semmelweis University Department of Traumatology Uzsoki u. 29-41 H-1145 Budapest Hungary
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The present study evaluated the histological changes in the muscle tissue after limb lengthening in skeletally immature rabbits and assessed the effect of different lengthening rates on the regeneration and degeneration properties of striated muscle. Thirteen different lengthening protocols were applied on a total of 16 male domestic white rabbits divided into four groups. The histopathological changes were analysed by a semiquantitative method according to the scoring system of Lee et al. (1993). After evaluation of the five main degenerative parameters (muscle atrophy, internalisation of muscle nuclei, degeneration of the muscle fibre, perimysial and endomysial fibrosis, haematomas), it is evident that rabbits subjected to limb lengthening at a rate of 3.2 mm/day showed more degenerative changes than those limb-lengthened at 0.8 or 1.6 mm/day. Our study showed that the regenerative mechanisms were not endless. If the daily lengthening rate reached the 3.2 mm/day limit, the regenerating ability of the muscle decreased, and signs of degeneration increased significantly.

  • Aronson, J. (1994): Temporal and spatial increases in blood flow during distraction osteogenesis. Clin. Orthop. 301, 124–131.

    Aronson J. , 'Temporal and spatial increases in blood flow during distraction osteogenesis ' (1994 ) 301 Clin. Orthop. : 124 -131.

    • Search Google Scholar
  • Caiozzo, V. J., Utkan, A., Chou, R., Khalafi, A., Chandra, H., Baker, M., Rourke, B., Adams, G., Baldwin, K. and Green, S. (2002): Effects of distraction on muscle length: Mechanisms involved in sarcomerogenesis. Clin. Orth. Rel. Resch. 403S, 133–145.

    Green S. , 'Effects of distraction on muscle length: Mechanisms involved in sarcomerogenesis ' (2002 ) 403S Clin. Orth. Rel. Resch. : 133 -145.

    • Search Google Scholar
  • Cooper, R. N., Tajbakhsh, S., Mouly, V., Cossu, G., Buckingham, M. and Butler-Browne, G. S. (1999): In vivo satellite cell activation via Myf5 and MyoD in regenerating mouse skeletal muscle. J. Cell Sci. 112, 2895–2901.

    Butler-Browne G. S. , 'In vivo satellite cell activation via Myf5 and MyoD in regenerating mouse skeletal muscle ' (1999 ) 112 J. Cell Sci. : 2895 -2901.

    • Search Google Scholar
  • De Deyne, P., Hayatsu, K., Meyer, R., Paley, D. and Herzenberg, J. (1999): Muscle regeneration and fiber-type transformation during distraction osteogenesis. J. Orthop. Res. 17, 560–570.

    Herzenberg J. , 'Muscle regeneration and fiber-type transformation during distraction osteogenesis ' (1999 ) 17 J. Orthop. Res. : 560 -570.

    • Search Google Scholar
  • Engel, A. G. and Banker, B. Q. (eds) (1986): Myology. McGraw-Hill Book Company, New York.

    '', in Myology , (1986 ) -.

  • Hawke, T. J. and Garry, D. J. (2001): Myogenic satellite cells: physiology to molecular biology. J. Appl. Physiol. 91, 534–551.

    Garry D. J. , 'Myogenic satellite cells: physiology to molecular biology ' (2001 ) 91 J. Appl. Physiol. : 534 -551.

    • Search Google Scholar
  • Hayatsu, K. and De Deyne, P. G. (2001): Muscle adaptation during distraction osteogenesis in skeletally immature and mature rabbits. J. Orthop. Res. 19, 897–905.

    Deyne P. G. , 'Muscle adaptation during distraction osteogenesis in skeletally immature and mature rabbits ' (2001 ) 19 J. Orthop. Res. : 897 -905.

    • Search Google Scholar
  • Jones, D. A. and Round, J. M. (1990): Skeletal Muscle in Health and Disease. A Textbook of Muscle Physiology. Manchester University Press, Manchester and New York, USA. 221 pp.

    Round J. M. , '', in Skeletal Muscle in Health and Disease. A Textbook of Muscle Physiology , (1990 ) -.

  • Lee, D. Y., Choi, I. H., Chung, C. Y., Chung, P. H., Chi, J. G. and Suh, Y. L. (1993): Effect of tibial lengthening on the gastrocnemius muscle. A histopathologic and morphometric study in rabbits. Acta Orthop. Scand. 64, 688–692.

    Suh Y. L. , 'Effect of tibial lengthening on the gastrocnemius muscle. A histopathologic and morphometric study in rabbits ' (1993 ) 64 Acta Orthop. Scand. : 688 -692.

    • Search Google Scholar
  • Leung, K. S., Cheung, W. H., Yeung, H. Y., Lee, K. M. and Fung, K. P. (2004): Effect of weight bearing on bone formation during distraction osteogenesis. Clin. Orthop. 419, 251–257.

    Fung K. P. , 'Effect of weight bearing on bone formation during distraction osteogenesis ' (2004 ) 419 Clin. Orthop. : 251 -257.

    • Search Google Scholar
  • Lindsey, C. A., Makarov, M. R., Shoemaker, S., Birch, J. G., Buschang, P. H., Cherkashin, A. M., Welch, R. D. and Samchukov, M. L. (2002): The effect of the amount of limb lengthening on skeletal muscle. Clin. Orthop. Relat. Res. 402, 278–287.

    Samchukov M. L. , 'The effect of the amount of limb lengthening on skeletal muscle ' (2002 ) 402 Clin. Orthop. Relat. Res. : 278 -287.

    • Search Google Scholar
  • Makarov, M. R., Kochutina, L. N., Samchukov, M. L., Birch, J. G. and Welch, R. D. (2001): Effect of rhythm and level of distraction on muscle structure. Clin. Orth. Relat. Res. 384, 250–264.

    Welch R. D. , 'Effect of rhythm and level of distraction on muscle structure ' (2001 ) 384 Clin. Orth. Relat. Res. : 250 -264.

    • Search Google Scholar
  • Mastalgia, F. L., Dawkins, R. L. and Papadimitrou, J. M. (1975): Morphological changes in skeletal muscle after transplantation: a light electron microscopic study of the initial phases of degeneration and regeneration. J. Neurol. Sci. 25, 227–247.

    Papadimitrou J. M. , 'Morphological changes in skeletal muscle after transplantation: a light electron microscopic study of the initial phases of degeneration and regeneration ' (1975 ) 25 J. Neurol. Sci. : 227 -247.

    • Search Google Scholar
  • Meffert, R. H., Tis, J. E., Inoue, N., McCarthy, E. F., Brug, E. and Chao, E. Y. S. (2000): Primary resective shortening followed by distraction osteogenesis for limb reconstruction: a comparison with simple lengthening. J. Orthop. Res. 18, 629–636.

    Chao E. Y. S. , 'Primary resective shortening followed by distraction osteogenesis for limb reconstruction: a comparison with simple lengthening ' (2000 ) 18 J. Orthop. Res. : 629 -636.

    • Search Google Scholar
  • Schultz, E. and McCormick, K. M. (1994): Skeletal muscle satellite cells. Rev. Physiol. Biochem. Pharmacol. 123, 213–257.

    McCormick K. M. , 'Skeletal muscle satellite cells ' (1994 ) 123 Rev. Physiol. Biochem. Pharmacol. : 213 -257.

    • Search Google Scholar
  • Schumacher, B., Keller, J. and Hvid, I. (1994): Distraction effects on muscle. Leg lengthening studied in rabbits. Acta Orthop. Scand. 65, 647–650.

    Hvid I. , 'Distraction effects on muscle. Leg lengthening studied in rabbits ' (1994 ) 65 Acta Orthop. Scand. : 647 -650.

    • Search Google Scholar
  • Shisha, T., Kiss, S., Pap, K., Simpson, H. and Szőke, G. (2007): Relative ability of young and adult muscles to respond to limb lengthening. J. Bone Joint Surg. [Br] 88, 1666–1669.

    Szőke G. , 'Relative ability of young and adult muscles to respond to limb lengthening ' (2007 ) 88 J. Bone Joint Surg. [Br] : 1666 -1669.

    • Search Google Scholar
  • Simpson, A. H., Williams, P. E., Kyberd, P., Goldspink, G. and Kenwright, J. (1995): The response of muscle to leg lengthening. J. Bone Joint Surg. [Br] 77, 630–636.

    Kenwright J. , 'The response of muscle to leg lengthening ' (1995 ) 77 J. Bone Joint Surg. [Br] : 630 -636.

    • Search Google Scholar
  • Tatsumi, R., Sheehan, S. M., Iwasaki, H., Hattori, A. and Allen, R. E. (2001): Mechanical stretch induces activation of skeletal muscle satellite cells in vitro. Exp. Cell Res. 267, 107–114.

    Allen R. E. , 'Mechanical stretch induces activation of skeletal muscle satellite cells in vitro ' (2001 ) 267 Exp. Cell Res. : 107 -114.

    • Search Google Scholar
  • Williams, P., Simpson, H., Kyberd, P., Kenwright, J. and Goldspink, G. (1999): Effect of rate of distraction on loss of range of joint movement, muscle stiffness, and intramuscular connective tissue content during surgical limb-lengthening: a study in the rabbit. Anat. Rec. 255, 78–83.

    Goldspink G. , 'Effect of rate of distraction on loss of range of joint movement, muscle stiffness, and intramuscular connective tissue content during surgical limb-lengthening: a study in the rabbit ' (1999 ) 255 Anat. Rec. : 78 -83.

    • Search Google Scholar

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