View More View Less
  • 1 Hungarian Academy of Sciences Institute for Veterinary Medical Research, Centre for Agricultural Research Hungária krt. 21 H-1143 Budapest Hungary
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

Enteropathogenic Escherichia coli (EPEC) bacteria frequently cause severe enteric diseases primarily in children and in young rabbits. Their pathogenicity for pigs has been tested by oral infection of colostrum-deprived newborn, and of severely immunosuppressed weaned pigs, but colonisation of conventional weaned pigs by porcine EPEC has not been experimentally studied. EPEC show similarities to enterohaemorrhagic E. coli (EHEC) additionally carrying shiga toxin genes integrated into the chromosome by lambdoid phages. We have demonstrated earlier that the porcine EPEC prototype strain P86-1390 (O45) could be transduced in vivo (in ligated loops of weaned pigs), by Stx2 phage derived from a human EHEC. Thus, the ability of this porcine EPEC strain to colonise conventional weaned pigs under farming conditions became a question of relevance to human health. To clarify this question, four intragastric infection experiments were performed on a total of 95 conventional weaned pigs. The EPEC P86-1390 and other well-characterised porcine EPEC strains were applied to 54 pigs, leaving 41 weaned pigs as negative controls. In three experiments moderate predispositions were applied: coinfections with enterotoxigenic E. coli (ETEC) or with low-virulence TGE coronavirus, application of fumonisin B1 with a normal therapeutic dose of dexamethasone, and the increase of soybean protein concentration in the feed. A total of 41 weaned pigs served as negative controls inoculated with a commensal porcine E. coli. Housing conditions simulated the farm environment. As an overall result, ileal segments of 18.5% of infected pigs were shown to be colonised by EPEC, while no EPEC were detected in the ilea of controls. Among predisposing factors occurring on farms, feed protein content increased by 20% (26.3% crude protein, provided by 48% soybean meal) seemed to enhance EPEC colonisation and resulted in the mobilisation of spontaneous latent EPEC/ETEC infection. The results indicate that under normal farm conditions porcine EPEC may colonise conventional weaned pigs by inducing ileal attaching effacing (AE) lesions with reasonable frequency, without clinical signs. The results also suggest that conventional weaned pigs may represent undetected reservoirs of porcine EPEC, potentially giving rise to the emergence of new types of EHEC due to natural transduction by Stx phages.

  • Anon. (2011): Seven E. coli O45 cases linked to smoked meat. Food Safety News Presented by Marler Calk LLP, PS. www.foodsafetynews.com/2011

  • Bielaszewska, M., Mellmann, A., Zhang, W., Köck, R., Fruth, A., Bauwens, A., Peters, G. and Karch, H. (2011): Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study. Lancet Infect. Dis. 11, 671–676.

    Karch H. , 'Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study ' (2011 ) 11 Lancet Infect. Dis. : 671 -676.

    • Search Google Scholar
  • Bouhet, S., Le Dorze, E., Peres, S., Fairbrother, J. M. and Oswald, I. P. (2006): Mycotoxin fumonisin B1 selectively down-regulates the basal IL-8 expression in pig intestine: in vivo and in vitro studies. Food Chem. Toxicol. 44, 1768–1773.

    Oswald I. P. , 'Mycotoxin fumonisin B1 selectively down-regulates the basal IL-8 expression in pig intestine: in vivo and in vitro studies ' (2006 ) 44 Food Chem. Toxicol. : 1768 -1773.

    • Search Google Scholar
  • Cornick, N. A. and VuKhac, H. (2008): Indirect transmission of E. coli O157:H7 occurs readily among swine but not among sheep. Appl. Environ. Microbiol. 74, 2488–2491.

    VuKhac H. , 'Indirect transmission of E. coli O157:H7 occurs readily among swine but not among sheep ' (2008 ) 74 Appl. Environ. Microbiol. : 2488 -2491.

    • Search Google Scholar
  • Frank, C., Faber, M. S., Askar, M., Bernard, H., Fruth, A., Gilsdorf, A., Hohle, M., Karch, H., Krause, G., Prager, R., Spode, A., Stark, K. and Werber, D., on behalf of the HUS investigation team (2011): Large and ongoing outbreak of haemolytic uraemic syndrome, Germany, May 2011. Euro Surveill. 16(21):pii=19878.

    Werber D. , 'Large and ongoing outbreak of haemolytic uraemic syndrome, Germany, May 2011 ' (2011 ) 16 Euro Surveill. : pii=19878 -.

    • Search Google Scholar
  • Ferens, W. A. and Hoyde, C. J. (2011): Escherichia coli O157:H7: animal reservoir and sources of human infection. Foodborne Pathogen Dis. 8, 465–487.

    Hoyde C. J. , 'Escherichia coli O157:H7: animal reservoir and sources of human infection ' (2011 ) 8 Foodborne Pathogen Dis. : 465 -487.

    • Search Google Scholar
  • Fröhlicher, E., Krause, G., Zweifel, C., Beutin, L. and Stephan, R. (2008): Characterization of attaching and effacing Escherichia coli (AEEC) isolated from pigs and sheep. BMC Microbiol. 8, 144. doi:10.1186/1471-2180-8-144.

    Stephan R. , 'Characterization of attaching and effacing Escherichia coli (AEEC) isolated from pigs and sheep ' (2008 ) 8 BMC Microbiol. : 144 -.

    • Search Google Scholar
  • Girard, F., Oswald, I. P., Taranu, I., Helie, P., Appleyard, G. D., Harel, J. and Fairbrother, J. M. (2005): Host immune status influences the development of attaching and effacing lesions in weaned pigs. Infect. Immun. 73, 5514–5523.

    Fairbrother J. M. , 'Host immune status influences the development of attaching and effacing lesions in weaned pigs ' (2005 ) 73 Infect. Immun. : 5514 -5523.

    • Search Google Scholar
  • Goffaux, F., China, B., Janssen, L. and Mainil, J. (2000): Genotypic characterization of enteropathogenic Escherichia coli (EPEC) isolated in Belgium from dogs and cats. Res. Microbiol. 151, 865–871.

    Mainil J. , 'Genotypic characterization of enteropathogenic Escherichia coli (EPEC) isolated in Belgium from dogs and cats ' (2000 ) 151 Res. Microbiol. : 865 -871.

    • Search Google Scholar
  • Helie, P., Morin, M., Jacques, M. and Fairbrother, J. M. (1991): Experimental infection of newborn pigs with an attaching and effacing Escherichia coli O45:K”E65” strain. Infect. Immun. 59, 814–821.

    Fairbrother J. M. , 'Experimental infection of newborn pigs with an attaching and effacing Escherichia coli O45:K”E65” strain ' (1991 ) 59 Infect. Immun. : 814 -821.

    • Search Google Scholar
  • Janke, B. H., Francis, D. H., Collins, J. E., Libal, M. C., Zeman, D. H. and Johnson, D. D. (1989): Attaching effacing Escherichia coli infections in calves, pigs, lambs and dogs. J. Vet. Diagn. Invest. 1, 6–11.

    Johnson D. D. , 'Attaching effacing Escherichia coli infections in calves, pigs, lambs and dogs ' (1989 ) 1 J. Vet. Diagn. Invest. : 6 -11.

    • Search Google Scholar
  • Kovács, M., Vetési, F., Kovács, F., Bata, Á., Horn, P., Tóth, Á. and Romvári, R. (2000): Experiment to determine limits of tolerance for fumonisin B1 in weaned piglets. J. Vet. Med. B 47, 277–286.

    Romvári R. , 'Experiment to determine limits of tolerance for fumonisin B1 in weaned piglets ' (2000 ) 47 J. Vet. Med. B : 277 -286.

    • Search Google Scholar
  • Krause, G., Zimmermann, S. and Beutin, L. (2005): Investigation of domestic animals and pets as a reservoir for intimin-(eae) gene positive Escherichia coli types. Vet. Microbiol. 106, 87–95.

    Beutin L. , 'Investigation of domestic animals and pets as a reservoir for intimin-(eae) gene positive Escherichia coli types ' (2005 ) 106 Vet. Microbiol. : 87 -95.

    • Search Google Scholar
  • Mainil, J., Janssen, L., Charlier, G., Jacquemin, E., China, B. and Goffaux, F. (2000): Les souches pathogènes d’Escherichia coli chez les chiens et chats: (II) Données cliniques et bactériologiques sur les souches entéropathogènes. Ann. Méd. Vét. 144, 335–343.

    Goffaux F. , 'Les souches pathogènes d’Escherichia coli chez les chiens et chats: (II) Données cliniques et bactériologiques sur les souches entéropathogènes ' (2000 ) 144 Ann. Méd. Vét. : 335 -343.

    • Search Google Scholar
  • Malik, A., Tóth, I., Beutin, L., Schmidt, H., Taminiau, B., Dow, M. A., Morabito, S., Oswald, E., Mainil, J. and Nagy, B. (2006): Serotypes and intimin types of intestinal and faecal strains of eae+ Escherichia coli from weaned pigs. Vet. Microbiol. 114, 82–93.

    Nagy B. , 'Serotypes and intimin types of intestinal and faecal strains of eae+ Escherichia coli from weaned pigs ' (2006 ) 114 Vet. Microbiol. : 82 -93.

    • Search Google Scholar
  • Moon, H. W., Sorensen, D. K. and Sautter, J. H. (1968): Experimental enteric colibacillosis in piglets. Can. J. Comp. Med. 32, 493–497.

    Sautter J. H. , 'Experimental enteric colibacillosis in piglets ' (1968 ) 32 Can. J. Comp. Med. : 493 -497.

    • Search Google Scholar
  • Moon, H. W., Whipp, S. C., Argenzio, R. A., Levine, M. M. and Gianella, R. A. (1983): Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infect. Immun. 53, 1340–1351.

    Gianella R. A. , 'Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines ' (1983 ) 53 Infect. Immun. : 1340 -1351.

    • Search Google Scholar
  • Nagy, B., Casey, T. A. and Moon, H. W. (1990): Phenotype and genotype of Escherichia coli isolated from pigs with postweaning diarrhea in Hungary. J. Clin. Microbiol. 28, 651–653.

    Moon H. W. , 'Phenotype and genotype of Escherichia coli isolated from pigs with postweaning diarrhea in Hungary ' (1990 ) 28 J. Clin. Microbiol. : 651 -653.

    • Search Google Scholar
  • Nagy, B., Nagy, G., Meder, M. and Mocsári, E. (1996): Enterotoxigenic Escherichia coli, rotavirus, porcine epidemic diarrhoea virus, adenovirus and calici-like virus in porcine postweaning diarrhoea in Hungary. Acta Vet. Hung. 44, 9–19.

    Mocsári E. , 'Enterotoxigenic Escherichia coli, rotavirus, porcine epidemic diarrhoea virus, adenovirus and calici-like virus in porcine postweaning diarrhoea in Hungary ' (1996 ) 44 Acta Vet. Hung. : 9 -19.

    • Search Google Scholar
  • Nagy, B., Whipp, S. C., Imberechts, H., Bertschinger, H. U., Dean-Nystrom, E. A., Casey, T. A. and Salajka, E. (1997): Biological relationship between F18ab and F18ac fimbriae of enterotoxigenic and verotoxigenic Escherichia coli from weaned pigs with oedema disease or diarrhoea. Microb. Pathog. 22, 1–11.

    Salajka E. , 'Biological relationship between F18ab and F18ac fimbriae of enterotoxigenic and verotoxigenic Escherichia coli from weaned pigs with oedema disease or diarrhoea ' (1997 ) 22 Microb. Pathog. : 1 -11.

    • Search Google Scholar
  • Nataro, J. P. and Kaper, J. B. (1998): Diarrhoeagic Escherichia coli. Clin. Microbiol. Rev. 11, 142–201.

    Kaper J. B. , 'Diarrhoeagic Escherichia coli ' (1998 ) 11 Clin. Microbiol. Rev. : 142 -201.

  • Pritchard, G. C. (1987): Transmissible gastroenteritis in endemically infected breeding herds of pigs in East Anglia, 1981–85. Vet. Rec. 120, 226–230.

    Pritchard G. C. , 'Transmissible gastroenteritis in endemically infected breeding herds of pigs in East Anglia, 1981–85 ' (1987 ) 120 Vet. Rec. : 226 -230.

    • Search Google Scholar
  • Schmidt, H., Bielaszewska, M. and Karch, H. (1999): Transduction of enteric Escherichia coli isolates with a derivative of Shiga toxin 2-encoding bacteriophage ϕ3538 isolated from Escherichia coli O157:H7. Appl. Environ. Microbiol. 65, 3855–3861.

    Karch H. , 'Transduction of enteric Escherichia coli isolates with a derivative of Shiga toxin 2-encoding bacteriophage ϕ3538 isolated from Escherichia coli O157:H7 ' (1999 ) 65 Appl. Environ. Microbiol. : 3855 -3861.

    • Search Google Scholar
  • Szmolka, A., Anjum, M. F., La Ragione, R., Kaszanyitzky, E. J. and Nagy, B. (2012): Microarray based comparative genotyping of gentamicin resistant Escherichia coli strains from food animals and humans. Vet. Microbiol. 156, 110–118.

    Nagy B. , 'Microarray based comparative genotyping of gentamicin resistant Escherichia coli strains from food animals and humans ' (2012 ) 156 Vet. Microbiol. : 110 -118.

    • Search Google Scholar
  • Tóth, I., Schmidt, H., Kardos, G., Lancz, Z., Creuzburg, K., Damjanova, I., Pászti, J., Beutin, L. and Nagy, B. (2009): Virulence genes and molecular typing of different groups of Escherichia coli O157 strains in cattle. Appl. Environ. Microbiol. 75, 6282–6291.

    Nagy B. , 'Virulence genes and molecular typing of different groups of Escherichia coli O157 strains in cattle ' (2009 ) 75 Appl. Environ. Microbiol. : 6282 -6291.

    • Search Google Scholar
  • Trabulsi, L. R., Keller, R. and Gomes, T. A. T. (2002): Typical and atypical enteropathogenic Escherichia coli. Emerg. Infect. Dis. 8, 508–513.

    Gomes T. A. T. , 'Typical and atypical enteropathogenic Escherichia coli ' (2002 ) 8 Emerg. Infect. Dis. : 508 -513.

    • Search Google Scholar
  • Wada, Y., Nakaoka, Y., Kondo, H., Nakazawa, M. and Kubo, M. (1996): Dual infection with attaching and effacing Escherichia coli and enterotoxigenic Escherichia coli in post-weaning pigs. J. Comp. Pathol. 114, 93–99.

    Kubo M. , 'Dual infection with attaching and effacing Escherichia coli and enterotoxigenic Escherichia coli in post-weaning pigs ' (1996 ) 114 J. Comp. Pathol. : 93 -99.

    • Search Google Scholar
  • Yin, X., Chambers, J. R., Wheatcroft, R., Johnson, R. P., Liu, B. and Gyles, C. L. (2009): Adherence of Escherichia coli O157:H7 mutants in vitro and in ligated pig intestines. Appl. Environ. Microbiol. 75, 4975–4983.

    Gyles C. L. , 'Adherence of Escherichia coli O157:H7 mutants in vitro and in ligated pig intestines ' (2009 ) 75 Appl. Environ. Microbiol. : 4975 -4983.

    • Search Google Scholar
  • Zhu, C., Harel, J., Jacques, M., Desautels, C., Donnenberg, M. S., Beaudry, M. and Fairbrother, J. M. (1994): Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea. Infect. Immun. 62, 4153–4159.

    Fairbrother J. M. , 'Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea ' (1994 ) 62 Infect. Immun. : 4153 -4159.

    • Search Google Scholar

Author information is available in PDF.
Please, download the file from HERE.

The manuscript preparation instructions is available in PDF.
Please, download the file from HERE.

Senior editors

Editor-in-Chief: Mária BENKŐ

Managing Editor: András SZÉKELY

Editorial Board

  • Béla DÉNES (National Food Chain Safety Office, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (National Agricultural Innovation Centre, Herceghalom, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 467 4081 (ed.-in-chief) or (36 1) 213 9793 (editor) Fax: (36 1) 467 4076 (ed.-in-chief) or (36 1) 213 9793

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture, Biology and Environmental Sciences
  • Elsevier Science Navigator
  • Focus On: Veterinary Science and Medicine
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

2020  
Total Cites 987
WoS
Journal
Impact Factor
0,955
Rank by Veterinary Sciences 101/146 (Q3)
Impact Factor  
Impact Factor 0,920
without
Journal Self Cites
5 Year 1,164
Impact Factor
Journal  0,57
Citation Indicator  
Rank by Journal  Veterinary Sciences 93/166 (Q3)
Citation Indicator   
Citable 49
Items
Total 49
Articles
Total 0
Reviews
Scimago 33
H-index
Scimago 0,395
Journal Rank
Scimago Veterinary (miscellaneous) Q2
Quartile Score  
Scopus 355/217=1,6
Scite Score  
Scopus General Veterinary 73/183 (Q2)
Scite Score Rank  
Scopus 0,565
SNIP  
Days from  145
sumbission  
to acceptance  
Days from  150
acceptance  
to publication  
Acceptance 19%
Rate

 

2019  
Total Cites
WoS
798
Impact Factor 0,991
Impact Factor
without
Journal Self Cites
0,897
5 Year
Impact Factor
1,092
Immediacy
Index
0,119
Citable
Items
59
Total
Articles
59
Total
Reviews
0
Cited
Half-Life
9,1
Citing
Half-Life
9,2
Eigenfactor
Score
0,00080
Article Influence
Score
0,253
% Articles
in
Citable Items
100,00
Normalized
Eigenfactor
0,09791
Average
IF
Percentile
42,606
Scimago
H-index
32
Scimago
Journal Rank
0,372
Scopus
Scite Score
335/213=1,6
Scopus
Scite Score Rank
General Veterinary 62/178 (Q2)
Scopus
SNIP
0,634
Acceptance
Rate
18%

 

Acta Veterinaria Hungarica
Publication Model Hybrid
Submission Fee none
Article Processing Charge 1100 EUR/article
Printed Color Illustrations 40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2021 Online subsscription: 696 EUR / 872 USD
Print + online subscription: 804 EUR / 1004 USD
Subscription fee 2022 Online subsscription: 710 EUR / 892 USD
Print + online subscription: 824 EUR / 1028 USD
Subscription Information Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation
1951
Publication
Programme
2020 Volume 68
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Apr 2021 0 1 2
May 2021 4 1 1
Jun 2021 5 2 3
Jul 2021 10 0 0
Aug 2021 7 1 1
Sep 2021 1 0 0
Oct 2021 0 0 0