Acta Veterinaria Hungarica
Volume/Issue:
Volume 60: Issue 3
Characterisation of cocaine- and amphetamine- regulated transcript-like immunoreactive (CART-LI) enteric neurons in the porcine small intestine
Authors: Joanna Wojtkiewicz 1 , Sławomir Gonkowski 3 , Marek Bladowski 2 , and Mariusz Majewski 2
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  • 1 University of Warmia and Mazury Division of Neurosurgery, Department of Neurology and Neurosurgery ul. Warszawska 30 10-082 Olsztyn Poland
  • 2 University of Warmia and Mazury Department of Human Physiology, Faculty of Medical Sciences Olsztyn Poland
  • 3 University of Warmia and Mazury Department of Clinical Physiology, Faculty of Veterinary Medicine Olsztyn Poland
DOI:
https://doi.org/10.1556/avet.2012.032
Page Count:
371–381
Publication Date:
01 Sep 2012
Online Publication Date:
17 Aug 2012
Article Category:
Research Article
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The aim of this study was to investigate the distribution and the number of cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) neurons and the co-localisation of CART with substance P (SP), somatostatin (SOM), nitric oxide synthase (NOS) and vasoactive intestinal polypeptide (VIP) within the enteric nervous system (ENS) in the porcine small intestine. Accordingly, the myenteric plexus (MP), outer submucous plexus (OSP) and inner submucous plexus (ISP) of the small intestine (duodenum, jejunum and ileum) were studied by double-labelling immunofluorescence technique. CART-LI neurons were observed in all gut fragments and all types of intramural plexuses studied and amounted from 0.2 ± 0.1% in the ISP of ileum to 22.4 ± 2.4% in the MP of this segment. The co-localisation of CART and NOS or/and VIP was observed depending on the segment of the gut and the complexity of the intramural plexus. On the other hand, during this study the co-localisation of CART and SOM or/and SP was not observed. The present study, for the first time, presents a detailed description of the CART distribution pattern and co-localisation with other neuromodulators within the ENS of the porcine small intestine.

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Cover Acta Veterinaria Hungarica
Acta Veterinaria Hungarica
Print ISSN:
0236-6290
Online ISSN:
1588-2705
Keywords:
Cocaine- and amphetamine-regulated transcript (CART); small intestine; pig; immunohistochemistry; enteric nervous system

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