Authors:
Jianan Li China Agricultural University National Animal Protozoa Laboratory, College of Veterinary Medicine Yuanmingyuan West Road 2, Haidian District Beijing 100193 China

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Jun Zou China Agricultural University National Animal Protozoa Laboratory, College of Veterinary Medicine Yuanmingyuan West Road 2, Haidian District Beijing 100193 China

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Guangwen Yin China Agricultural University National Animal Protozoa Laboratory, College of Veterinary Medicine Yuanmingyuan West Road 2, Haidian District Beijing 100193 China

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Xianyong Liu China Agricultural University National Animal Protozoa Laboratory, College of Veterinary Medicine Yuanmingyuan West Road 2, Haidian District Beijing 100193 China

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Xun Suo China Agricultural University National Animal Protozoa Laboratory, College of Veterinary Medicine Yuanmingyuan West Road 2, Haidian District Beijing 100193 China

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Eimerian coccidia are the most common parasitic organisms infecting chickens. The feasibility of genetic manipulation of these parasites via electroporation is proven, but this method is cumbersome and time consuming. Here we report our endeavour to develop a rapid and simple transfection method by gene gun. Tungsten particles coated with plasmid DNA encoding enhanced yellow fluorescent protein (EYFP) were used for the bombardment of Eimeria maxima unsporulated oocysts. Seven Mpa (1015 psi) helium pressure, 65 mm target distance and −0.098 Mpa (24.8″ Hg) chamber vacuum were the optimised parameters for bombardment. After sporulation, the bombarded oocysts were inoculated into chickens, and the progeny oocysts were checked under fluorescent microscope and subjected to genomic DNA extraction, which was used either for polymerase chain reaction (PCR) amplification or plasmid rescue assay. Although the expression of EYFP was not observed, the gene was amplified from both genomic DNA and the rescued plasmid, suggesting that the plasmid DNA existed in the form of episome. These results are encouraging for the genetic processing of the sporogony stage of eimerian parasites.

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Senior editors

Editor-in-Chief: Ferenc BASKA

Editorial assistant: Szilvia PÁLINKÁS

 

Editorial Board

  • Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
  • Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • János GÁL (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA

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Phone: (36 20) 560 4183 (ed.-in-chief) or (36 1) 478 4100/8430 (editor)

E-mail: acta.veterinaria@univet.hu (ed.-in-chief)

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2023  
Web of Science  
Journal Impact Factor 0.7
Rank by Impact Factor Q3 (Veterinary Sciences)
Journal Citation Indicator 0.4
Scopus  
CiteScore 1.8
CiteScore rank Q2 (General Veterinary)
SNIP 0.39
Scimago  
SJR index 0.258
SJR Q rank Q3

Acta Veterinaria Hungarica
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Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation
1951
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

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