The aim of the study was to determine the magnitude of diurnal variability of serum adiponectin in healthy beagle dogs, and the possible roles of feeding and glucocorticoids on adiponectin concentrations. For this, adiponectin was measured at 8:00, 10:00, 12:00, 16:00, 20:00, 24:00 and at 4:00 h in 4 beagle dogs that were fasted on the day of the experiment and in 4 dogs that were fed as usual at 9:00 h. Diurnal variability in serum adiponectin concentrations was negligible in both the fed and the fasted dogs. To study the possible effect of glucocorticoids on adiponectin, beagle dogs (n = 14) were assigned to one of three experimental groups. Dogs of the control group were injected with 0.1 ml/kg 0.9% NaCl subcutaneously, while dogs of Groups 1 and 2 were injected with 1 mg/kg and 5 mg/kg of methylprednisolone, respectively, and adiponectin was measured at 8:00, 10:00, 12:00, 16:00 and 20:00 h. Average serum adiponectin levels were not significantly different before and after methylprednisolone exposure at different time-points in the two treated groups. In conclusion, no evidence of postprandial changes in adiponectin level or effects of single-dose glucocorticoid administration on adiponectin were observed in the present study.