Vitrification of early avian blastodermal cells with a new type of cryocontainer

Eszter Patakiné Várkonyi; Gabriella Horváth; Nikoletta Sztán; Éva VÁradi; Judit Barna

doi:10.1556/avet.2012.044

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Acta Veterinaria Hungarica

Volume/Issue:: Volume 60: Issue 4

Vitrification of early avian blastodermal cells with a new type of cryocontainer

Authors:

Eszter Patakiné Várkonyi

Eszter Patakiné Várkonyi Institute for Small Animal Research and Co-ordination Centre for Gene Conservation Isaszegi út 200 H-2100 Gödöllő Hungary

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Gabriella Horváth

Gabriella Horváth Institute for Small Animal Research and Co-ordination Centre for Gene Conservation Isaszegi út 200 H-2100 Gödöllő Hungary

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Nikoletta Sztán

Nikoletta Sztán Institute for Small Animal Research and Co-ordination Centre for Gene Conservation Isaszegi út 200 H-2100 Gödöllő Hungary

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Éva VÁradi

Éva VÁradi Institute for Small Animal Research and Co-ordination Centre for Gene Conservation Isaszegi út 200 H-2100 Gödöllő Hungary

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, and

Judit Barna

Judit Barna Institute for Small Animal Research and Co-ordination Centre for Gene Conservation Isaszegi út 200 H-2100 Gödöllő Hungary

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Pages:: 501–509

Online Publication Date:: 16 Nov 2012

Publication Date:: 01 Dec 2012

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/avet.2012.044

Keywords:: Vitrification; blastodermal cells; chicken; avian; cryocontainer

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Although cryopreservation of avian semen is only applicable for singlegene traits, cryopreservation of avian blastodermal cells could facilitate preservation of the entire genome of endangered or rare-breed poultry. Slow freezing methods result in acceptable survival rates; however, there are apparently no reports regarding the use of vitrification. The aim of the study was to establish methods for chicken embryonic cell vitrification, including development of a container which supported cryopreservation of large numbers of cells (to increase the probability of chimera production). Based on a preliminary study, vitrification seemed to be practical for avian blastodermal cell preservation. Pieces of mosquito net as carrier increased live cell rates compared to pellet form in media containing two macromolecules. Furthermore, we concluded that fetal calf serum in the vitrification medium could be replaced by polyvinylpyrrolidone, a chemically defined substance free of unwanted growth factors and potential pathogens.

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Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language	English
Size	A4
Year of Foundation	1951
Volumes per Year	1
Issues per Year	4
Founder	Magyar Tudományos Akadémia
Founder's Address	H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher	Akadémiai Kiadó
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Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-6290 (Print)
ISSN	1588-2705 (Online)