The aim of this study was to describe long-term follow-up and difference in immune reactions in the tear film following penetrating keratoplasty (PK) in horses when differently preserved corneas were utilised. This report describes for the first time the use of corneal grafts preserved in tissue culture media in equine PK. Eight experimental horses with normal eyes were included and freshly harvested, frozen or preserved corneal grafts were used for the PK. The graft-taking technique and storage, PK surgery, postoperative treatments and complications are described. The mean postoperative follow-up time was 286 days. Tear film samples taken before and periodically after surgery were measured for IgM, IgG and IgA contents by direct ELISA. All grafts were incorporated into the donor horse but were rejected to some degree. The differently harvested corneal grafts healed in the same manner and looked similar. Preoperatively, the clear corneas meant low risk for graft failure, and the fresh or stored tissues provided intact endothelium, although there were no clear graft sites postoperatively. The presence of IgA, IgG and IgM was demonstrated in the tear film from the early postoperative period. IgG levels were lower than IgA or IgM and had a constant baseline in every case, as IgA and IgM had great variability with time and an individual pattern in each eye.
Andrew, S. E. and Brooks, D. E. (1999): The success and complication rate of penetrating keratoplasty in the horse. Invest. Ophthalmol. Vis. Sci. 40, 253–257.
Brooks D. E., 'The success and complication rate of penetrating keratoplasty in the horse' (1999) 40Invest. Ophthalmol. Vis. Sci.: 253-257.
Brooks D. E.The success and complication rate of penetrating keratoplasty in the horseInvest. Ophthalmol. Vis. Sci.199940253257)| false
Andrew, S. E. and Willis, A. M. (2005): Diseases of the cornea and sclera. In: Gilger, B. C. (ed.) Equine Ophthalmology. First edition. Elsevier Saunders, St. Louis, Missouri. pp. 157–251.
Willis A. M., '', in Equine Ophthalmology, (2005) -.
Willis A. M.Equine Ophthalmology2005)| false
Andrew, S. E., Brooks, D. E., Biros, D. J., Denis, H. M., Cutler, T. J. and Gelatt, K. N. (2000): Posterior lamellar keratoplasty for treatment of deep stromal abscesses in nine horses. Vet. Ophthalmol. 3, 99–103.
Gelatt K. N., 'Posterior lamellar keratoplasty for treatment of deep stromal abscesses in nine horses' (2000) 3Vet. Ophthalmol.: 99-103.
Gelatt K. N.Posterior lamellar keratoplasty for treatment of deep stromal abscesses in nine horsesVet. Ophthalmol.2000399103)| false
Kerényi, Á., Nagy, Gy., Veres, A., Varga, L., Füst, Á., Nagymihály, A., Czumbel, N., Süveges, I. and Füst, Gy. (2002): C1r-C1s-C1inhibitor (C1rs-C1inh) complex measurements in tears of patients before and after penetrating keratoplasty. Curr. Eye Res. 24, 99–104.
Füst Gy., 'C1r-C1s-C1inhibitor (C1rs-C1inh) complex measurements in tears of patients before and after penetrating keratoplasty' (2002) 24Curr. Eye Res.: 99-104.
Füst Gy.C1r-C1s-C1inhibitor (C1rs-C1inh) complex measurements in tears of patients before and after penetrating keratoplastyCurr. Eye Res.20022499104)| false
Strubbe, D. T., Brooks, D. E., Schultz, G. S., Willis-Goulet, H., Gelatt, K. N., Andrew, S. T., Kallberg, M. E., Mackay, E. O. and Collante, W. R. (2000): Evaluation of tear film proteinases in horses with ulcerative keratitis. Vet. Ophthalmol. 3, 111–119.
Collante W. R., 'Evaluation of tear film proteinases in horses with ulcerative keratitis' (2000) 3Vet. Ophthalmol.: 111-119.
Collante W. R.Evaluation of tear film proteinases in horses with ulcerative keratitisVet. Ophthalmol.20003111119)| false