Ultrastructural lesions and immunohistochemical analysis of Bcl-2 protein expression in the kidney of chickens with experimental ochratoxicosis

Carmen Solcan; Dorina Timofte; Viorel Floristean; Stuart Carter; Gheorghe Solcan

doi:10.1556/avet.2013.021

Acta Veterinaria Hungarica

Volume/Issue:: Volume 61: Issue 3

Ultrastructural lesions and immunohistochemical analysis of Bcl-2 protein expression in the kidney of chickens with experimental ochratoxicosis

Authors:

Carmen Solcan

Carmen Solcan University of Agricultural Sciences and Veterinary Medicine Faculty of Veterinary Medicine Mihail Sadoveanu Alley 8 700489 Iasi Romania

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Dorina Timofte

Dorina Timofte

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Viorel Floristean

Viorel Floristean University of Agricultural Sciences and Veterinary Medicine Faculty of Veterinary Medicine Mihail Sadoveanu Alley 8 700489 Iasi Romania

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Stuart Carter

Stuart Carter University of Liverpool School of Veterinary Science Liverpool UK

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Gheorghe Solcan

Gheorghe Solcan University of Agricultural Sciences and Veterinary Medicine Faculty of Veterinary Medicine Mihail Sadoveanu Alley 8 700489 Iasi Romania

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Pages:: 344–353

Online Publication Date:: 24 Mar 2013

Publication Date:: 01 Sep 2013

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/avet.2013.021

Keywords:: Chickens; ochratoxicosis; kidney; Bcl-2; immunohistochemistry; electron microscopy; adenoma-like structures

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A study was conducted to evaluate the nephrotoxic effect of ochratoxin A (OTA) in broiler chickens. Forty Ross 308 broilers (6 days old) were divided into two groups: one group received daily, by gavage, ochratoxin A at a daily dose of 50 μg/kg body weight for up to 21 days, while the control group received only diluent (sunflower oil). After 21 days, the chickens were euthanised and the kidneys removed for analysis by histopathology and immunohistochemistry to detect an anti-apoptotic marker (Bcl-2), and by transmission electron microscopy. Macroscopically the kidneys were enlarged, showing degeneration and gout deposits. Histologically, glomerulonephrosis and tubulonephrosis were common lesions in all chicks. In two of the five chicks exposed to OTA for 21 days, focal tubular cell proliferation, multiple adenoma-like structures and Bcl-2-positive epithelial cells were identified in layers of the renal papilla and in convoluted tubules. Transmission electron microscopy of the proximal convoluted tubules identified abnormal forms of mitochondria. The nephrotoxic effect of ochratoxicosis in chickens is probably due to carcinogenic changes induced in the epithelial tissues.

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Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language	English
Size	A4
Year of Foundation	1951
Volumes per Year	1
Issues per Year	4
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ISSN	0236-6290 (Print)
ISSN	1588-2705 (Online)