View More View Less
  • 1 Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, Dois Irmãos, CEP 52171-900 Recife, Pernambuco, Brazil
  • 2 University Estadual do Maranhão, São Luís, Brazil
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

Exposure of caprine oocytes and embryos to retinoids enhances embryonic development, but the mechanisms governing this phenomenon have not been characterised. The aim of the present study was to evaluate if the incidence of apoptosis is affected by the addition of retinyl acetate (RAc) and 9-cis-retinoic acid (RA) during in vitro maturation (IVM) of caprine oocytes. Embryonic development was recorded on days 3 and 8 post-fertilisation, and apoptosis was measured by caspase activity and DNA fragmentation (TUNEL assay). Control zygotes had lower capacity to cleave and reach the blastocyst stage (24.45 ± 2.32 and 5.32 ± 0.81, respectively) than those of RAc- (29.96 ± 1.62 and 7.94 ± 0.93, respectively) and RA-treated groups (30.12 ± 1.51 and 7.36 ± 1.02, respectively). Oocytes and blastocysts positive for TUNEL assay were more frequent, respectively, in the controls (8.20 ± 0.78, 8.70 ± 1.05) than in RAc (5.60 ± 0.52, 4.80 ± 0.51) and RA (6.40 ± 0.69, 5.40 ± 0.69). Caspase activity did not differ between control oocytes (7.20 ± 0.91), RAc (6.60 ± 0.68) and RA (7.30 ± 0.67), but it was reduced in RAc- (5.05 ± 0.62) and RA-treated blastocysts (5.75 ± 0.22) compared to controls (8.35 ± 0.71). These results indicate that the addition of retinoids during IVM increases the developmental potential of goat embryos with a concomitant reduction in apoptosis rates.

  • Abazari-Kia, A. H., Mohammadi-Sangcheshmeh, A., Dehghani-Mohammadabadi, M., Jamshidi-Adegani, F., Veshkini, A., Zhandi, M., Cinar, M. U. and Salehi, M. (2014): Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte. J. Assist. Reprod. Genet. 31, 313321.

    • Search Google Scholar
    • Export Citation
  • Almiñana, C., Gil, M. A., Cuello, C., Caballero, I., Roca, J., Vazquez, J. M., Gomez, E. and Martinez, E. A. (2008): In vitro maturation of porcine oocytes with retinoids improves embryonic development. Reprod. Fertil. Dev. 20, 483489.

    • Search Google Scholar
    • Export Citation
  • Anguita, B., Paramio, M. T., Morató, R., Romaguera, R., Jiménez-Macedo, A. R., Mogas, T. and Izquierdo, D. (2009): Effect of the apoptosis rate observed in oocytes and cumulus cells on embryo development in prepubertal goats. Anim. Reprod. Sci. 116, 95106.

    • Search Google Scholar
    • Export Citation
  • Baldassarre, H. and Karatzas, C. N. (2004): Advanced assisted reproduction technologies (ART) in goats. Anim. Reprod. Sci. 82–83, 255266.

    • Search Google Scholar
    • Export Citation
  • Baldassarre, H., Wang, B., Keefer, C. L., Lazaris, A. and Karatzas, C. N. (2004): State of the art in the production of transgenic goats. Reprod. Fertil. Dev. 16, 465470.

    • Search Google Scholar
    • Export Citation
  • Bormann, C. L., Ongeri, E. M. and Krisher, R. L. (2003): The effect of vitamins during maturation of caprine oocytes on subsequent developmental potential in vitro. Theriogenology 59, 13731380.

    • Search Google Scholar
    • Export Citation
  • Cavalcanti Neto, C. C. (2004): Use of retinol for the in vitro production of goat embryos [in Portuguese]. Thesis, Belo Horizonte, Universidade Federal de Minas Gerais, Brazil.

    • Export Citation
  • Chiamenti, A., Aguiar Filho, C. R., Freitas Neto, L. M., Chaves, R. M., Paula-Lopes, F., Lima, P. F., Gonçalves, P. B. D. and Oliveira, M. A. L. (2010): Effects of retinoids on the in vitro development of Capra hircus embryos to blastocysts in two different culture systems. Reprod. Domest. Anim. 45, 6872.

    • Search Google Scholar
    • Export Citation
  • Chiamenti, A., Aguiar Filho, C. R., Moura, M. T., Paula-Lopes, F. F., Neves, J. P., Cavalcanti Neto, C. C., Gonçalves, P. B. D., Lima, P. F. and Oliveira, M. A. L. (2013): Use of retinyl acetate, retinoic acid and insulin-like growth factor-I (IGF-I) to enhance goat embryo production. Acta Vet. Hung. 61, 116124.

    • Search Google Scholar
    • Export Citation
  • Deb, G. K., Dey, S. R., Bang, J. I., Cho, S. J., Park, H. C., Lee, J. G. and Kong, I. K. (2011): 9-cis retinoic acid improves developmental competence and embryo quality during in vitro maturation of bovine oocytes through the inhibition of oocyte tumor necrosis factor-α gene expression. J. Anim. Sci. 89, 27592767.

    • Search Google Scholar
    • Export Citation
  • Deb, G. K., Dey, S. R., Bang, J. I., Lee, J. G. and Kong, I. K. (2012): 9-cis retinoic acid inhibits cumulus cell apoptosis during the maturation of bovine cumulus-oocyte-complexes. J. Anim. Sci. 90, 17981806.

    • Search Google Scholar
    • Export Citation
  • Duque, P., Diez, C., Royo, L., Lorenzo, P. L., Carneiro, G., Hidalgo, C. O., Facal, N. and Gomez, E. (2002 a): Enhancement of developmental capacity of meiotically inhibited bovine oocytes by retinoic acid. Mol. Hum. Reprod. 17, 27062714.

    • Search Google Scholar
    • Export Citation
  • Duque, P., Gomez, E., Hidalgo, C., Facal, N., Fernandez, I. and Diez, C. (2002 b): Retinoic acid during in vitro maturation of bovine oocytes promotes embryonic development and early differentiation. Theriogenology 57, 364.

    • Search Google Scholar
    • Export Citation
  • Gjørret, J. O., Knijn, H. M., Dieleman, S. J., Avery, B., Larsson, L. I. and Maddox-Hyttel, P. (2003): Chronology of apoptosis in bovine embryos produced in vivo and in vitro. Biol. Reprod. 69, 11931200.

    • Search Google Scholar
    • Export Citation
  • Gómez, E., Royo, L. J., Duque, P., Carneiro, G., Hidalgo, C., Goyache, F., Lorenzo, P. L., Alvarez, I., Facal, N. and Díez, C. (2003): 9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells. Mol. Reprod. Dev. 66, 247255.

    • Search Google Scholar
    • Export Citation
  • Hammami, S., Izquierdo, D., Catalá, M. G., Paramio, M. T. and Morató, R. (2014): In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A. Animal 8, 94101.

    • Search Google Scholar
    • Export Citation
  • Hidalgo, C., Diez, C., Duque, P., Facal, N., Prendes, J. M., Fernandez, I. and Gomez, E. (2002): Improved cumulus-oocyte complex yields from heifers treated with retinol. Theriogenology 57, 672.

    • Search Google Scholar
    • Export Citation
  • Hofmann, C. and Eichele, G. (1994): The retinols, biology, chemistry and medicine. In: Sporn, M. B., Roberts, A. B. and Googman, D. S. (eds) Retinoids in Development. Raven Press, New York, NY. pp. 387441.

    • Search Google Scholar
    • Export Citation
  • Keefer, C. L., Baldassarre, H., Keyston, R., Wang, B., Bhatia, B., Bilodeau, A. S., Zhou, J. F., Leduc, M., Downey, B. R., Lazaris, A. and Karatzas, C. N. (2001): Generation of dwarf goat (Capra hircus) clones following nuclear transfer with transfected and nontransfected fetal fibroblasts and in vitro-matured oocytes. Biol. Reprod. 64, 849856.

    • Search Google Scholar
    • Export Citation
  • Keskintepe, L., Simplício, A. A. and Brackett, B. G. (1998): Caprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders. Theriogenology 49, 12651274.

    • Search Google Scholar
    • Export Citation
  • Kwong, P. J., Abdullah, R. B. and Wan Khadijah, W. E. (2012): Increasing glucose in KSOMaa basal medium on culture Day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer. Theriogenology 78, 921929.

    • Search Google Scholar
    • Export Citation
  • Liang, S., Kang, J., Jin, H., Liu, X., Li, J., Li, S., Lu, Y., Wang, W. and Yin, X. J. (2012): The influence of 9-cis-retinoic acid on nuclear and cytoplasmic maturation and gene expression in canine oocytes during in vitro maturation. Theriogenology 77, 11981205.

    • Search Google Scholar
    • Export Citation
  • Lima, P. F., Oliveira, M. A. L., Gonçalves, P. B. D., Montagner, M. M., Reichenbach, H-D., Weppert, M., Cavalcanti Neto, C. C., Pina, V. M. R. and Santos, M. H. B. (2004): Effects of retinol on the in vitro development of Bos indicus embryos to blastocysts in two different culture systems. Reprod. Domest. Anim. 39, 356360.

    • Search Google Scholar
    • Export Citation
  • Lima, P. F., Oliveira, M. A. L., Santos, M. H. B., Reichenbach, H-D., Weppert, M., Paula-Lopes, F., Cavalcanti Neto, C. C. and Gonçalves, P. B. D. (2006): Effect of retinoids and growth factor on in vitro bovine embryos produced under chemically defined conditions. Anim. Reprod. Sci. 95, 184192.

    • Search Google Scholar
    • Export Citation
  • Mem, H., Monson, R. L., Parrish, J. J. and Rutledge, J. J. (2003): Degeneration of cryopreserved bovine oocytes via apoptosis during subsequent culture. Cryobiology 47, 7381.

    • Search Google Scholar
    • Export Citation
  • Paula-Lopes, F. F. and Hansen, P. J. (2002 a): Apoptosis is an adaptative response in bovine preimplantation embryos that facilitates survival after heat shock. Biochem. Biophys. Res. Commun. 295, 3742.

    • Search Google Scholar
    • Export Citation
  • Paula-Lopes, F. F. and Hansen, P. J. (2002 b): Heat shock-induced apoptosis in preimplantation bovine embryos is a developmentally regulated phenomenon. Biol. Reprod. 66, 11691177.

    • Search Google Scholar
    • Export Citation
  • Perez, G. I., Tao, X. J. and Tilly, J. L. (1999): Fragmentation and death (a.k.a. apoptosis) of ovulated oocytes. Mol. Hum. Reprod. 5, 414420.

    • Search Google Scholar
    • Export Citation
  • Romaguera, R., Casanovas, A., Morató, R., Izquierdo, D., Catalá, M., Jimenez-Macedo, A. R., Mogas, T. and Paramio, M. T. (2010): Effect of follicle diameter on oocyte apoptosis, embryo development and chromosomal ploidy in prepubertal goats. Theriogenology 74, 364373.

    • Search Google Scholar
    • Export Citation
  • Roth, Z. and Hansen, P. J. (2004): Involvement of apoptosis in disruption of developmental competence of bovine oocytes by heat shock during maturation. Biol. Reprod. 71, 18981906.

    • Search Google Scholar
    • Export Citation
  • Shaw, D. W., Farin, P. W., Washburn, S. P. and Britt, J. H. (1995): Effect of retinol palmitate on ovulation rate and embryo quality in superovulated cattle. Theriogenology 44, 5158.

    • Search Google Scholar
    • Export Citation
  • Van Blerkom, J. and Davis, P. W. (1998): DNA strand break and phosphatidylserine redistribution in newly ovulated and culture mouse and human oocytes: occurrence and relationship to apoptosis. Hum. Reprod. 13, 13171324.

    • Search Google Scholar
    • Export Citation
  • Xu, J. S., Chang, T. M., Chan, T. H., Ho, P. C. and Yeung, W. S. B. (2001): The incidence of cytoplasmic fragmentation in mouse embryos in vitro is not affected by inhibition of caspase activity. Fertil. Steril. 5, 986991.

    • Search Google Scholar
    • Export Citation
  • Zhang, H., Wu, B., Liu, H., Qiu, M., Liu, J., Zhang, Y. and Quan, F. (2013): Improving development of cloned goat embryos by supplementing α-lipoic acid to oocyte in vitro maturation medium. Theriogenology 80, 228233.

    • Search Google Scholar
    • Export Citation

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 1 0
Jul 2020 4 2 0
Aug 2020 3 0 0
Sep 2020 0 0 0
Oct 2020 1 0 0
Nov 2020 4 0 0
Dec 2020 0 0 0