Francisella tularensis is a Gram-negative bacterium, the causative agent of the zoonotic disease tularaemia. The bacterium has developed several extracellular and intracellular strategies to evade the hosts’ innate and adaptive immune responses. The aims of the study were to examine complement sensitivity of wild and attenuated F. tularensis ssp. holarctica strains in animal hosts of distinct sensitivity to the bacterium, to compare the complement-evading ability of wild strains of different phylogeographic background, and to examine the role of factor H in the host–pathogen interactions. Complement sensitivity assays were carried out on various F. tularensis ssp. holarctica wild strains and on the attenuated live vaccine strain (LVS) with sera of the highly sensitive house mouse (Mus musculus), the moderately sensitive European brown hare (Lepus europaeus) and the relatively resistant cattle (Bos taurus). Specific binding of complement regulator factor H to bacterial membrane proteins was examined by Western blot assays. All wild strains interacted with the hosts’ complement system and showed no significant differences in their survivability. The attenuated LVS was resistant to serum killing in mouse, but was lysed in the sera of hare and cattle. Direct binding of factor H to F. tularensis membrane proteins was not detected.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
Antwerpen, M. H., Schacht, E., Kaysser, P. and Splettstoesser, W. D. (2013): Complete genome sequence of a Francisella tularensis subsp. holarctica strain from Germany causing lethal infection in common marmosets. Genome Announc. 1, e00135-12. doi:10.1128/genomeA.00135-12.
-
Ariza-Miguel, J., Johansson, A., Fernández-Natal, M. I., Martínez-Nistal, C., Orduña, A., Rodríguez-Ferri, E. F., Hernández, M. and Rodríguez-Lázaro, D. (2014): Molecular investigation of tularemia outbreaks, Spain, 1997–2008. Emerg. Infect. Dis. 20, 754. doi:http://dx.doi.org/10.3201/eid2005.130654.
-
Bhide, M. R., Escudero, R., Camafeita, E., Gil, H., Jado, I. and Anda, P. (2009): Complement factor H binding by different Lyme disease and relapsing fever Borrelia in animals and human. BMC Res. Notes 2, 134. doi:10.1186/1756-0500-2-134.
-
Biedzka-Sarek, M., Jarva, H., Hyytiäinen, H., Meri, S. and Skurnik, M. (2008): Characterization of complement factor H binding to Yersinia enterocolitica serotype O: 3. Infect. Immun. 76, 4100–4109.
-
Chen, W., KuoLee, R., Shen, H. and Conlan, J. W. (2004): Susceptibility of immunodeficient mice to aerosol and systemic infection with virulent strains of Francisella tularensis. Microb. Pathog. 36, 211–218.
-
Clinton, S. R., Bina, J. E., Hatch, T. P., Whitt, M. A. and Miller, M. A. (2010): Binding and activation of host plasminogen on the surface of Francisella tularensis. BMC Microbiol. 10, 76. doi:http://www.biomedcentral.com/1471-2180/10/76.
-
Crane, D. D., Warner, S. L. and Bosio, C. M. (2009): A novel role for plasmin-mediated degradation of opsonizing antibody in the evasion of host immunity by virulent, but not attenuated, Francisella tularensis. J. Immunol. 183, 4593–4600.
-
Dempsey, M. P., Dobson, M., Zhang, C., Zhang, M., Lion, C., Gutiérrez-Martín, C. B., Iwen, P. C., Fey, P. D., Olson, M. E., Niemeyer, D., Francesconi, S., Crawford, R., Stanley, M., Rhodes, J., Wagner, D. M., Vogler, A. J., Birdsell, D., Keim, P., Johansson, A., Hinrichs, S. H. and Benson, A. K. (2007): Genomic deletion marking an emerging subclone of Francisellatularensis subsp. holarctica in France and the Iberian Peninsula. Appl. Environ. Microbiol. 73, 7465–7470.
-
Elkins, K. L., Cowley, S. C. and Bosio, C. M. (2003): Innate and adaptive immune responses to an intracellular bacterium, Francisella tularensis live vaccine strain. Microbes Infect. 5, 135–142.
-
Feldman, K. A. (2003): Tularemia. JAVMA-J. Am. Vet. Med. A 222, 725–730.
-
Gyuranecz, M., Birdsell, D. N., Splettstoesser, W., Seibold, E., Beckstrom-Sternberg, S. M., Makrai, L., Fodor, L., Fabbi, M., Vicari, N., Johansson, A., Busch, J. D., Vogler, A. J., Keim, P. and Wagner, D. M. (2012 a): Phylogeography of Francisella tularensis subsp. holarctica, Europe. Emerg. Infect. Dis. 18, 290–293.
-
Gyuranecz, M., Reiczigel, J., Krisztalovics, K., Monse, L., Szabóné, G. K., Szilágyi, A., Szépe, B., Makrai, L., Magyar, T., Bhide, M. and Erdélyi, K. (2012 b): Factors influencing emergence of tularemia, Hungary, 1984–2010. Emerg. Infect. Dis. 18, 1379–1380.
-
Gyuranecz, M., Szeredi, L., Makrai, L., Fodor, L., Mészáros, Á. R., Szépe, B., Füleki, M. and Erdélyi, K. (2010): Tularemia of European brown hare (Lepus europaeus) A pathological, histopathological, and immunohistochemical study. Vet. Pathol. 47, 958–963.
-
Hestvik, G., Warns-Petit, E., Smith, L. A., Fox, N. J., Uhlhorn, H., Artois, M., Hannant, D., Hutchings, M. R., Mattsson, R., Yon, L. and Gavier-Widen, D. (2014): The status of tularemia in Europe in a one-health context: a review. Epidemiol. Infect. 30, 1–24.
-
Janeway, C. A. Jr., Travers, P., Walport, M. and Shlomchik, M. J. (2001): The complement system and innate immunity. In: Immunobiology: The Immune System in Health and Disease. 5th edition. Garland Science, New York. Available from: http://www.ncbi.nlm.nih.gov/books/NBK27100/
-
Jones, C. L., Napier, B. A., Sampson, T. R., Llewellyn, A. C., Schroeder, M. R. and Weiss, D. S. (2012): Subversion of host recognition and defense systems by Francisella spp. Microbiol. Mol. Biol. Rev. 76, 383–404.
-
Keim, P., Johansson, A. and Wagner, D. M. (2007): Molecular epidemiology, evolution, and ecology of Francisella. Ann. N. Y. Acad. Sci. 1105, 30–66.
-
Maraha, B., Hajer, G., Sjödin, A., Forsman, M., Paauw, A., Roeselers, G., Verspui, E., Frenay, I., Notermans, D., de Vries, M. and Reubsaet, F. (2013): Indigenous infection with Francisellatularensis holarctica in the Netherlands. Case Rep. Infect. Dis. Article ID 916985.doi: http://dx.doi.org/10.1155/2013/916985.
-
Meri, T., Amdahl, H., Lehtinen, M. J., Hyvärinen, S., McDowell, J. V., Bhattacharjee, A., Meri, S., Marconi, R., Goldman, A. and Jokiranta, T. S. (2013): Microbes bind complement inhibitor factor H via a common site. PLoS Pathog. 9, e1003308. doi:10.1371/journal.ppat.1003308.
-
Molins, C. R., Delorey, M. J., Yockey, B. M., Young, J. W., Sheldon, S. W., Reese, S. M., Schriefer, M. E. and Petersen, J. M. (2010): Virulence differences among Francisella tularensis subsp. tularensis clades in mice. PLoS One 5, e10205. doi: 10.1371/journal.pone.0010205.
-
Mörner, T. and Sandstedt, K. (1983): A serological survey of antibodies against Francisella tularensis in some Swedish mammals. Nord. Vet. Med. 35, 82–85.
-
Nasr, A. B. and Klimpel, G. R. (2008): Subversion of complement activation at the bacterial surfacepromotes serum resistance and opsonophagocytosis of Francisella tularensis. J. LeukocyteBiol. 84, 77–85.
-
Origgi, F., Frey, J. and Pilo, P. (2014): Characterisation of a new group of Francisella tularensis subsp. holarctica in Switzerland with altered antimicrobial susceptibilities, 1996 to 2013.Euro. Surveill. 19, pii=20858. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=20858.
-
Vogler, A. J., Birdsell, D., Price, L. B., Bowers, J. R., Beckstrom-Sternberg, S. M., Auerbach, R. K., Beckstrom-Sternberg, J. S., Johansson, A., Clare, A., Buchhagen, J. L., Petersen, J. M., Pearson, T., Vaissaire, J., Dempsey, M. P., Foxall, P., Engelthaler, D. M., Wagner, D. M. and Keim, P. (2009): Phylogeography of Francisella tularensis: global expansion of a highly fit clone. J. Bacteriol. 191, 2474–2484.
-
Yu, J. J., Raulie, E. K., Murthy, A. K., Guentzel, M. N., Klose, K. E. and Arulanandam, B. P. (2008): The presence of infectious extracellular Francisella tularensis subsp. novicida in murine plasma after pulmonary challenge. Eur. J. Clin. Microbiol. 27, 323–325.
-
Zarrella, T. M., Singh, A., Bitsaktsis, C., Rahman, T., Sahay, B., Feustel, P. J., Gosselin, E. J., Sellati, T. J. and Hazlett, K. R. O. (2011): Host-adaptation of Francisella tularensis alters the bacterium’s surface-carbohydrates to hinder effectors of innate and adaptive immunity. PLoS One 6, e22335. doi:10.1371/journal.pone.0022335.
Monthly Content Usage
| Abstract Views | Full Text Views | PDF Downloads | |
|---|---|---|---|
| Aug 2020 | 2 | 1 | 1 |
| Sep 2020 | 1 | 0 | 0 |
| Oct 2020 | 0 | 0 | 0 |
| Nov 2020 | 3 | 0 | 0 |
| Dec 2020 | 0 | 0 | 0 |
| Jan 2021 | 0 | 0 | 0 |
| Feb 2021 | 0 | 0 | 0 |
Copyright Akadémiai Kiadó
AKJournals is the trademark of Akadémiai Kiadó's journal publishing business branch.
Copyright Akadémiai Kiadó AKJournals is the trademark of Akadémiai Kiadó's journal publishing business branch.
Powered by PubFactory