View More View Less
  • 1 University of Pannonia, Deák F. u. 16, H-8360 Keszthely, Hungary
  • 2 University of Pannonia, Veszprém, Hungary
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

The aim of the present pilot study was to apply a flow cytometric assay, the so-called OxyDNA test, to determine the level of oxidative DNA damage in fish spermatozoa exposed to different concentrations (0.01–10,000 mg/L) of cadmium. Milt was collected from three randomly selected Prussian carp (Carassius auratus gibelio) males. Oxidative DNA damage was assessed with the OxyDNA kit and using flow cytometry. The ratio of OxyDNA-positive events increased significantly at higher cadmium concentrations. The results indicate that direct contact of fish spermatozoa with cadmium-polluted water initiates genotoxic damage.

  • Aitken, R. J., De Iuliis, G. N., Gibb, Z. and Baker, M. A. (2012): The Simmet lecture: new horizons on an old landscape –oxidative stress, DNA damage and apoptosis in the male germ line. Reprod. Dom. Anim. 47 (Suppl. 4), 714.

    • Search Google Scholar
    • Export Citation
  • Cabrita, E., Martínez-Páramo, S., Gavaia, P. J., Riesco, M. F., Valcarce, D. G., Sarasquete, C., Herráez, M. P. and Robles, V. (2014): Factors enhancing fish sperm quality and emerging tools for sperm analysis. Aquaculture 432, 389401.

    • Search Google Scholar
    • Export Citation
  • Dadoune, J. P. (1995): The nuclear status of human sperm cells. Micron 26, 323345.

  • De Iuliis, G. N., Thomson, L. K., Mitchell, L. A., Finnie, J. M., Koppers, A. J., Hedges, A., Nixon, B. and Aitken, R. J. (2009): DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2’-deoxyguanosine, a marker of oxidative stress. Biol. Reprod. 81, 517524.

    • Search Google Scholar
    • Export Citation
  • Evenson, D. P. (1999): Loss of livestock breeding efficiency due to uncompensable sperm nuclear defects. Reprod. Fertil. Dev. 11, 115.

    • Search Google Scholar
    • Export Citation
  • Evenson, D. P. and Wixon, R. (2005): Environmental toxicants cause sperm DNA fragmentation as detected by the Sperm Chromatin Structure Assay (SCSA). Toxicol. Appl. Pharmacol. 207, 532537.

    • Search Google Scholar
    • Export Citation
  • Evenson, D. P. and Wixon, R. (2006): Clinical aspects of sperm DNA fragmentation detection and male infertility. Theriogenology 65, 979991.

    • Search Google Scholar
    • Export Citation
  • Gregory, M., Aravindakshan, J., Nadialek, S. and Cyr, D. G. (2008): Effects of endocrine disrupting chemicals on testicular functions. In: Alavi, S. M. H., Cosson, J. J., Coward, K. and Rafiee, G. (eds) Fish Spermatology. Alpha Science International Ltd., Oxford, UK. pp. 161214.

    • Search Google Scholar
    • Export Citation
  • Hoornstra, D., Andersson, M. A., Mikkola, R. and Salkinoja-Salonen, M. S. (2003): A new method for in vitro detection of microbially produced mitochondrial toxins. Toxicology in Vitro 17, 745751.

    • Search Google Scholar
    • Export Citation
  • Kakasi, B., Balikó, T., Bercsényi, M., Merth, J. and Nagy, Sz. T. (2013): Determination of the LC50 value of cadmium in European perch (Perca fluviatilis) fingerlings [in Hungarian]. Proceedings of 50th Georgikon Scientific Conference, Keszthely, Hungary. p. 58.

    • Search Google Scholar
    • Export Citation
  • Oliva, R. (2006): Protamines and male infertility. Hum. Reprod. Update 12, 417435.

  • Olsen, A. K., Lindeman, B., Wiger, R., Duale, N. and Brunborg, G. (2005): How do male germ cells handle DNA damage? Toxicol. Appl. Pharmacol. 207, 521531.

    • Search Google Scholar
    • Export Citation
  • Sakkas, D., Mariethoz, E., Manicardi, G., Bizzaro, D., Bianchi, P. G. and Bianchi, U. (1999): Origin of DNA damage in ejaculated human spermatozoa. Rev. Reprod. 4, 3137.

    • Search Google Scholar
    • Export Citation
  • Silva, P. F., Gadella, B. M., Colenbrander, B. and Roelen, B. A. (2007): Exposure of bovine sperm to pro-oxidants impairs the developmental competence of the embryo after the first cleavage. Theriogenology 67, 609619.

    • Search Google Scholar
    • Export Citation
  • Sood, S., Malecki, I. A., Tawang, A. and Martin, G. B. (2012): Survival of emu (Dromaius novaehollandiae) sperm preserved at subzero temperatures and different cryoprotectant concentrations. Theriogenology 78, 15571569.

    • Search Google Scholar
    • Export Citation
  • Varner, D. D. (2008): Developments in stallion semen evaluation. Theriogenology 70, 448462.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 3 1
Jul 2020 1 1 2
Aug 2020 3 0 0
Sep 2020 3 0 0
Oct 2020 3 0 0
Nov 2020 6 2 0
Dec 2020 0 0 0