Acta Veterinaria Hungarica
Volume/Issue:
Volume 64: Issue 1
Mycoplasma and host interaction: In vitro gene expression modulation in Mycoplasma synoviae and infected chicken chondrocytes
Authors:
Ivanka Cizelj University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia

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Daliborka Dušanić University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia

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Dušan Benčina University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia

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Mojca Narat University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia

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Pages:
26–37
Online Publication Date:
Mar 2016
Publication Date:
01 Mar 2016
Article Category:
Research Article
DOI:
https://doi.org/10.1556/004.2016.003
Keywords:
Mycoplasma synoviae ; chicken chondrocytes; gene expression; pathogenicity; enzymes
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The complex interplay between Mycoplasma synoviae and chicken chondrocytes (CCH), which come into direct contact during infectious synovitis, has been examined at the level of gene expression. Our previous studies demonstrated a significant influence of M. synoviae on the level of CCH gene expression. Here, we show for the first time that in vitro co-cultivation of M. synoviae and CCH also induces upregulation of gene expression in this mycoplasma. We observed significantly increased expression of genes important for M. synoviae pathogenicity, including cysteine protease cysP, neuraminidase nanH, haemagglutinin vlhA, and the putative nuclease MS53_0284. Moreover, the pattern of gene expression was dependent on the infection environment. In CCH, significant changes in the expression of genes encoding catabolic enzymes of the cartilage extracellular matrix (cathepsins B, K and L, aggrecanase ADAM10, and matrix metalloproteinase MMP2) were demonstrated. Infection of CCH with M. synoviae also elevated the expression of the gene encoding peptidyl arginine deiminase, type III (PADI3), which is responsible for the post-translational citrullination of proteins.

  • Bartok, B. and Firestein, G. S. (2010): Fibroblast-like synoviocytes: key effector cells in rheumatoid arthritis. Immunol. Rev. 233, 233–255.

    • Search Google Scholar
    • Export Citation

  • Bercic, R. L., Cizelj, I., Dušanic, D., Narat, M., Zorman-Rojs, O., Dovc, P. and Bencina, D. (2011): Neuraminidase of Mycoplasma synoviae desialylates heavy chain of the chicken immunoglobulin G and glycoproteins of chicken tracheal mucus. Avian Pathol. 40, 299–308.

    • Search Google Scholar
    • Export Citation

  • Cecchini, K. R., Gorton, T. S. and Geary, S. J. (2007): Transcriptional responses of Mycoplasma gallisepticum strain R in association with eukaryotic cells. J. Bacteriol. 189, 5803–5807.

    • Search Google Scholar
    • Export Citation

  • Cizelj, I., Bercic, R. L., Dušanic, D., Narat, M., Kos, J., Dovc, P. and Bencina, D. (2011): Mycoplasma gallisepticum and Mycoplasma synoviae express a cysteine protease CysP, which can cleave chicken IgG into Fab and Fc. Microbiology 157, 362–372.

    • Search Google Scholar
    • Export Citation

  • Dušanic, D., Bencina, D., Oven, I., Cizelj, I., Bencina, M. and Narat, M. (2012): Mycoplasma synoviae induces upregulation of apoptotic genes, secretion of nitric oxide and appearance of an apoptotic phenotype in infected chicken chondrocytes. Vet. Res. 43, 7.

    • Search Google Scholar
    • Export Citation

  • Dušanic, D., Bercic, R. L., Cizelj, I., Salmic, S., Narat, M. and Bencina, D. (2009): Mycoplasma synoviae invades non-phagocytic chicken cells in vitro. Vet. Microbiol. 138, 114–119.

    • Search Google Scholar
    • Export Citation

  • Goldring, M. B. and Marcu, K. B. (2009): Cartilage homeostasis in health and rheumatic diseases. Arthritis Res. Ther. 11, 224.

  • Hallamaa, K. M., Tang, S. L., Ficorilli, N. and Browning, G. F. (2008): Differential expression of lipoprotein genes in Mycoplasma pneumoniae after contact with human lung epithelial cells, and under oxidative and acidic stress. BMC Microbiol. 8, 124.

    • Search Google Scholar
    • Export Citation

  • Kerr, K. M. and Olson, N. O. (1970): Pathology of chickens inoculated experimentally or contactinfected with Mycoplasma synoviae. Avian Dis. 14, 290–320.

    • Search Google Scholar
    • Export Citation

  • Lockaby, S. B., Hoerr, F. J., Lauerman, L. H. and Kleven, S. H. (1998): Pathogenicity of Mycoplasma synoviae in broiler chickens. Vet. Pathol. 35, 178–190.

    • Search Google Scholar
    • Export Citation

  • Morrow, C. J., Whithear, K. G. and Kleven, S. H. (1990): Restriction endonuclease analysis of Mycoplasma synoviae strains. Avian Dis. 34, 611–616.

    • Search Google Scholar
    • Export Citation

  • Narat, M., Bencina, D., Kleven, S. H. and Habe, F. (1998): The hemagglutination-positive phenotype of Mycoplasma synoviae induces experimental infectious synovitis in chickens more frequently than does the hemagglutination-negative phenotype. Infect. Immun. 66, 6004–6009.

    • Search Google Scholar
    • Export Citation

  • Noormohammadi, A. H., Markham, P. F., Whithear, K. G., Walker, I. D., Gurevich, V. A., Ley, D. H. and Browning, G. F. (1997): Mycoplasma synoviae has two distinct phase-variable major membrane antigens, one of which is a putative hemagglutinin. Infect. Immun. 65, 2542–2547.

    • Search Google Scholar
    • Export Citation

  • Novak, J. F., Hayes, J. D. and McMaster, J. H. (1995): Mycoplasma-mediated bone resorption in bone organ cultures. Microbios 81, 241–260.

    • Search Google Scholar
    • Export Citation

  • Pfaffl, M. W. (2001): A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29, e45.

  • Razin, S., Yogev, D. and Naot, Y. (1998): Molecular biology and pathogenicity of mycoplasmas. Microbiol. Mol. Biol. Rev. 62, 1094–1156.

    • Search Google Scholar
    • Export Citation

  • Rodwell, A. W. and Whitcomb, R. F. (1983): Methods for direct and indirect measurement of mycoplasma growth. In: Tully, J. G. and Razin, S. (eds) Methods in Mycoplasmology. Academic Press, New York–London. pp. 185–196.

    • Search Google Scholar
    • Export Citation

  • Uysal, H., Nandakumar, K. S., Kessel, C., Haag, S., Carlsen, S., Burkhardt, H. and Holmdahl, R. (2010): Antibodies to citrullinated proteins: molecular interactions and arthritogenicity. Immunol. Rev. 233, 9–33.

    • Search Google Scholar
    • Export Citation

  • Vasconcelos, A. T., Ferreira, H. B., Bizarro, C. V., Bonatto, S. L., Carvalho, M. O., Pinto, P. M., Almeida, D. F., Almeida, L. G., Almeida, R., Alves-Filho, L., Assuncao, E. N., Azevedo, V. A., Bogo, M. R., Brigido, M. M., Brocchi, M., Burity, H. A., Camargo, A. A., Camargo, S. S., Carepo, M. S., Carraro, D. M., de Mattos Cascardo, J. C., Castro, L. A., Cavalcanti, G., Chemale, G., Collevatti, R. G., Cunha, C. W., Dallagiovanna, B., Dambros, B. P., Dellagostin, O. A., Falcao, C., Fantinatti-Garboggini, F., Felipe, M. S., Fiorentin, L., Franco, G. R., Freitas, N. S., Frias, D., Grangeiro, T. B., Grisard, E. C., Guimaraes, C. T., Hungria, M., Jardim, S. N., Krieger, M. A., Laurino, J. P., Lima, L. F., Lopes, M. I., Loreto, E. L., Madeira, H. M., Manfio, G. P., Maranhao, A. Q., Martinkovics, C. T., Medeiros, S. R., Moreira, M. A., Neiva, M., Ramalho-Neto, C. E., Nicolas, M. F., Oliveira, S. C., Paixao, R. F., Pedrosa, F. O., Pena, S. D., Pereira, M., Pereira-Ferrari, L., Piffer, I., Pinto, L. S., Potrich, D. P., Salim, A. C., Santos, F. R., Schmitt, R., Schneider, M. P., Schrank, A., Schrank, I. S., Schuck, A. F., Seuanez, H. N., Silva, D. W., Silva, R., Silva, S. C., Soares, C. M., Souza, K. R., Souza, R. C., Staats, C. C., Steffens, M. B., Teixeira, S. M., Urmenyi, T. P., Vainstein, M. H., Zuccherato, L. W., Simpson, A. J. and Zaha, A. (2005): Swine and poultry pathogens: the complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae. J. Bacteriol. 187, 5568–5577.

    • Search Google Scholar
    • Export Citation

  • Vossenaar, E. R., Radstake, T. R., van der Heijden, A., van Mansum, M. A., Dieteren, C., de Rooij, D. J., Barrera, P., Zendman, A. J. and van Venrooij, W. J. (2004): Expression and activity of citrullinating peptidylarginine deiminase enzymes in monocytes and macrophages. Ann. Rheum. Dis. 63, 373–381.

    • Search Google Scholar
    • Export Citation

  • Yamada, R. (2005): Peptidylarginine deiminase type 4, anticitrullinated peptide antibodies, and rheumatoid arthritis. Autoimmun. Rev. 4, 201–206.

    • Search Google Scholar
    • Export Citation
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Cover Acta Veterinaria Hungarica
Acta Veterinaria Hungarica
Print ISSN:
0236-6290
Online ISSN:
1588-2705

Senior editors

Editor-in-Chief: Ferenc BASKA

Editorial assistant: Szilvia PÁLINKÁS

 

Editorial Board

  • Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
  • Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • János GÁL (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation		 1951
Volumes
per Year		 1
Issues
per Year		 4
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

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