View More View Less
  • 1 Wroclaw University of Environmental and Life Sciences, C. K. Norwida 31, 50375 Wroclaw, Poland
  • 2 Wroclaw University of Environmental and Life Sciences, C. K. Norwida 31, 50375 Wroclaw, Poland
  • 3 Warsaw University of Life Sciences (SGGW), Warsaw, Poland
  • 4 University of Life Sciences, Lublin, Poland
  • 5 Warmia and Mazury University in Olsztyn, Olsztyn, Poland
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

Primary heart tumours affect less than 1% of dogs. Due to their rare incidence, every research showing the frequency of cardiac tumours is valuable. Routine diagnostics is often complemented with immunohistochemical analysis. This study was conducted on 110 patient records from all veterinary faculties in Poland from dogs diagnosed with heart tumours between 1970 and 2014. The dogs’ age, breed and sex with tumour localisation and histopathological diagnosis were analysed. Because of its most common incidence, samples of haemangiosarcoma underwent further examination with assessment of the expression of cell markers that have not been evaluated earlier (i.e. minichromosome maintenance proteins and beta-catenin). We noted 111 tumours including 88.3% malignant and 10.8% benign ones. Haemangiosarcoma and aortic body tumour were the most frequent cardiac neoplasms in the dogs examined (45.9% and 27.9% of all tumours, respectively). Immunohistochemical analysis of haemangiosarcoma showed a positive expression of all markers examined. CD31, vimentin, and beta-catenin showed a positive reaction in all 11 samples examined. At least one proliferative marker (Ki-67, MCM-3 or MCM-7) showed a positive reaction in each sample. MCM-3 showed a higher expression than the two other proliferative markers (P = 0.006), but only Ki-67 showed a positive correlation with the mitotic index (P > 0.05, r = 0.89). Although beta-catenin, MCM-3 and MCM-7 showed a positive reaction in the haemangiosarcomas examined, their usefulness as diagnostic and prognostic factors should be a topic of further research.

  • Agaimy, A., Rösch, J., Weyand, M. and Strecker, T. (2012): Primary and metastatic cardiac sarcomas: a 12-year experience at a German heart centre. Int. J. Exp. Pathol. 5, 928938.

    • Search Google Scholar
    • Export Citation
  • Aupperle, H., März, I., Ellenberger, C., Buschatz, S., Reischauer, A. and Schoon, H.-A. (2007): Primary and secondary heart tumours in dogs and cats. J. Comp. Pathol. 136, 1826.

    • Search Google Scholar
    • Export Citation
  • Bertazzolo, W., Dell’Orco, M., Bonfanti, U., Ghisleni, G., Caniatti, M., Masserdotti, C., Antoniazzi, E., Crippa, L. and Roccabianca, P. (2005): Canine angiosarcoma: cytologic, histologic, and immunohistochemical correlations. Vet. Clin. Path. 34, 2834.

    • Search Google Scholar
    • Export Citation
  • Brown, N. O., Patnaik, A. K. and MacEwen, E. G. (1985): Canine hemangiosarcoma: retrospective analysis of 104 cases. JAVMA 186, 5658.

  • Ciaputa, R., Kandefer-Gola, M., Nowak, M. and Madej, J. A. (2013): Prevalence of tumours in domestic animals in the Lower Silesia (Poland) in 2009–2011. Bull. Vet. Inst. Pulawy 57, 5359.

    • Search Google Scholar
    • Export Citation
  • Ferrer, L., Fondevila, D., Rabanal, R. M. and Vilafranca, M. (1995): Immunohistochemical detection of CD31 antigen in normal and neoplastic canine endothelial cells. J. Comp. Path. 112, 319326.

    • Search Google Scholar
    • Export Citation
  • Galofaro, V., Rapisarda, G., Sfacteria, A., Lanteri, G. and Marino, F. (2012): Beta-catenin expression in pilomatrix carcinoma with multiple visceral metastases in a dog. Acta Vet. Hung. 60, 449457.

    • Search Google Scholar
    • Export Citation
  • Gambichler, T., Shtern, M., Rotterdam, S., Bechara, F. G., Stücker, M., Altmeyer, P. and Kreuter, A. (2009): Minichromosome maintenance proteins are useful adjuncts to differentiate between benign and malignant melanocytic skin lesions. J. Am. Acad. Dermatol. 60, 808813.

    • Search Google Scholar
    • Export Citation
  • Ge, Y., Ro, J. Y., Kim, D., Kim, C. H., Reardon, M. J., Blackmon, S., Zhai, J., Coffey, D., Benjamin, R. S. and Ayala, A. G. (2011): Clinicopathologic and immunohistochemical characteristics of adult primary cardiac angiosarcomas: analysis of 10 cases. Ann. Diagn. Pathol. 15, 262267.

    • Search Google Scholar
    • Export Citation
  • Gesek, M., Rotkiewicz, T., Otrocka-Domagala, I., Pazdzior-Czapula, K., Kozlowska, A., Korzeniowska, P., Grochowska, K. and Welenc, J. (2014): Manifestation of tumours in domestic animals in Warmia and Mazury (Poland) between 2003 and 2011. Bull. Vet. Inst. Pulawy 58, 439446.

    • Search Google Scholar
    • Export Citation
  • Kim, C. H., Dancer, J. Y., Coffey, D., Zhai, Q. J., Reardon, M., Ayala, A. and Ro, J. Y. (2008): Clinicopathologic study of 24 patients with primary cardiac sarcomas: a 10-year single institution experience. Hum. Pathol. 39, 933938.

    • Search Google Scholar
    • Export Citation
  • Kumar, N., Agarwal, S., Ahuja, A., Das, P., Airon, B. and Ray, R. (2012): Spectrum of cardiac tumors excluding myxoma: experience of a tertiary center with review of the literature. Pathol. Res. Pract. 207, 769774.

    • Search Google Scholar
    • Export Citation
  • Kreuter, A., Jesse, M., Potthoff, A., Brockmeyer, N. H., Gambichler, T., Stücker, M., Bechara, F. G., Pfister, H. and Wieland, U. (2009): Expression of proliferative biomarkers in anal intraepithelial neoplasia of HIV-positive men. J. Am. Acad. Dermatol. 63, 490498.

    • Search Google Scholar
    • Export Citation
  • Lam, K. Y., Dickens, P. and Chan, A. C. (1993): Tumors of the heart. A 20-year experience with a review of 12,485 consecutive autopsies. Arch. Pathol. Lab. Med. 117, 10271031.

    • Search Google Scholar
    • Export Citation
  • Lau, K. M., Chan, Q. K., Pang, J. C., Li, K. K., Yeung, W. W., Chung, N. Y., Lui, P. C., Tam, Y. S., Li, H. M., Zhou, L., Wang, Y., Mao, Y. and Ng, H. K. (2010): Minichromosome maintenance proteins 2, 3 and 7 in medulloblastoma: overexpression and involvement in regulation of cell migration and invasion. Oncogene 29, 54755489.

    • Search Google Scholar
    • Export Citation
  • Noszczyk-Nowak, A., Nowak, M., Paslawska, U., Atamaniuk, W. and Nicpon, J. (2010): Cases with manifestation of chemodectomai diagnosed in dogs in Department of Internal Diseases with Horses, Dog and Cat Clinic, Veterinary Medicine Faculty, University of Environmental and Life Sciences, Wroclaw, Poland. Acta Vet. Scand. 52, 35.

    • Search Google Scholar
    • Export Citation
  • Nowak, M., Madej, J. A. and Dziegiel, P. (2007 a): Expression of E-cadherin, ß-catenin and K antigeni and their reciprocal correlations in fibrosarcomas of soft tissues in dogs. In Vivo 21, 751756.

    • Search Google Scholar
    • Export Citation
  • Nowak, M., Madej, J. A. and Dziegiel, P. (2007 b): Expression of E-cadherin, ß-catenin and K antigen and their reciprocal relationships in mammary adenocarcinomas in bitches. Folia Histochem. Cytobiol. 45, 233238.

    • Search Google Scholar
    • Export Citation
  • Rajagopalan, V., Jesty, S. A., Craig, L. E. and Gompf, R. (2013): Comparison of presumptive echocardiographic and definitive diagnoses of cardiac tumors in dogs. J. Vet. Intern. Med. 27, 10921096.

    • Search Google Scholar
    • Export Citation
  • Remmele, W. and Stegner, H. E. (1987): Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue. Pathologe 8, 138140.

    • Search Google Scholar
    • Export Citation
  • Sabattini, S. and Bettini, G. (2009): An immunohistochemical analysis of canine haemangioma and haemangiosarcoma. J. Comp. Pathol. 140, 158168.

    • Search Google Scholar
    • Export Citation
  • Saydam, O., Senol, O., Schaaij-Visser, T. B. M., Pham, T. V., Piersma, S. R., Stemmer- Rachaminov, A. O., Wurdinger, T., Peerdeman, S. M. and Jimenez, C. R. (2010): Comparative protein profiling reveals minichromosome maintenance (MCM) proteins as novel potential tumor markers for meningiomas. J. Proteome Res. 9, 485494.

    • Search Google Scholar
    • Export Citation
  • Stein, T. J., Holmes, K. E., Muthuswamy, A., Thompson, V. and Huelsmeyer, M. K. (2011): Characterization of ß-catenin expression in canine osteosarcoma. Vet. Comp. Oncol. 9, 6573.

    • Search Google Scholar
    • Export Citation
  • Wang, J. G., Liu, H., Yu, W. J., Li, Y. J. and Xin, F. J. (2012): Primary cardiac neoplasms: a clinicopathologic analysis of 81 cases. Zhonghua Bing Li Xue Za Zhi [Chinese J. Pathol.] 41, 808812.

    • Search Google Scholar
    • Export Citation
  • Ware, W. A. and Hopper, D. L. (1999): Cardiac tumors in dogs: 1982–1995. J. Vet. Intern. Med. 13, 95103.