View More View Less
  • 1 University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary
  • 2 MTA-KE Mycotoxins in the Food Chain Research Group, Kaposvár, Hungary
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

The mycotoxin T-2 has many harmful effects on mammalian cells and reproductive functions. In the present study, the in vitro effect of T-2 toxin on mouse blastocysts was examined. Embryos were cultured in media supplemented with 0.5, 0.75 and 1 ng/ml T-2. Different exposure times were applied [96 h (treatment I) or 24 h following 72 h in toxin-free media (treatment II)]. Blastomere number, nuclear chromatin status and blastocoel formation were investigated in blastocysts. Our data show that the effect of T-2 toxin may vary depending on the stage of the embryo at the start of exposure. At 96 h of exposure, the blastocysts had blastomeres with normal chromatin quality but their developmental potential was decreased. After 24 h of exposure applied following a 72-h culture, blastomeres had a higher level of chromatin damage, although their developmental potential was the same as in the control embryos. In both cases, decreased mitotic rate was found, which resulted in decreased blastomere number even at low toxin concentration.

  • AbdelHafez, F., Xu, J., Goldberg, J. and Desai, N. (2011): Vitrification in open and closed carriers at different cell stages: assessment of embryo survival, development, DNA integrity and stability during vapor phase storage for transport.. BMC Biotechnol. 11, 110.

    • Search Google Scholar
    • Export Citation
  • Bennett, J. and Klich, M. (2003): Mycotoxins.. Clin. Microbiol. Rev. 16, 497516.

  • Beyer, M., Ferse, I., Mulac, D., Würthwein, E. and Humpf, H. (2009): Structural elucidation of T-2 toxin thermal degradation products and investigations toward their occurrence in retail food.. J. Agric. Food Chem. 575, 18671875.

    • Search Google Scholar
    • Export Citation
  • Biomin Holding GmbH (2015): Biomin Global Mycotoxin Survey 2015. https://info.biomin.net/acton/fs/blocks/showLandingPage/a/14109/p/p-004e/t/page/fm/17

  • Caloni, F., Ranzenigo, G., Cremonesi, F. and Spicer, L. J. (2009): Effects of a trichothecene, T-2 toxin, on proliferation and steroid production by porcine granulosa cells.. Toxicon 54, 337344.

    • Search Google Scholar
    • Export Citation
  • Dardik, A. and Shultz, R. (1991): Blastocoel expansion in the preimplantation mouse embryo: stimulatory effect of TGF-alpha and EGF.. Development 113, 919930.

    • Search Google Scholar
    • Export Citation
  • Glenn, A. E. (2007): Mycotoxigenic Fusarium species in animal feed.. Anim. Feed Sci. Technol. 137, 213240.

  • Heddle, J., Cimino, M., Hayashi, M., Romagna, F., Shelby, M. D., Tucker, J. D., Vanparys, P. and MacGregor, J. T. (1991): Micronuclei as an index of cytogenetic damage: past, present and future.. Environ. Mol. Mutagen. 18, 277291.

    • Search Google Scholar
    • Export Citation
  • Hnida, C., Engenheiro, E. and Ziebe, S. (2004): Computer-controlled, multilevel, morphometric analysis of blastomere size as biomarker of fragmentation and multinuclearity in human embryos.. Hum. Reprod. 19, 288293.

    • Search Google Scholar
    • Export Citation
  • Huszenicza, Gy. , Fekete, S., Szigeti, G., Kulcsár, M., Fébel, H., Kellems, R. O., Nagy, P., Cseh, S., Veresegyházy, T. and Hullár, I. (2000): Ovarian consequences of low dose peroral Fusarium (T-2) toxin in a ewe and heifer model.. Theriogenology 53, 16311639.

    • Search Google Scholar
    • Export Citation
  • Jackson, K., Ginsburg, E., Hornstein, M., Rein, M. and Clarke, R. (1998): Multinucleation in normally fertilized embryos is associated with an accelerated ovulation induction response and lower implantation and pregnancy rates in in vitro fertilization-embryo transfer cycles.. Fertil. Steril. 70, 6066.

    • Search Google Scholar
    • Export Citation
  • Kovács, M., Tornyos, G., Matics, Z., Kametler, L., Rajli, V., Bodnár, Z., Kulcsár, M., Huszenicza, G., Keresztes, Z. and Cseh, S. (2011): Subsequent effect of subacute T-2 toxicosis on spermatozoa, seminal plasma and testosterone production in rabbits.. Animal 5, 15631569.

    • Search Google Scholar
    • Export Citation
  • Lonergan, P., Fair, T., Corcoran, D. and Evans, A. (2006): Effect of culture environment on gene expression and developmental characteristics in IVF-derived embryos.. Theriogenology 65, 137152.

    • Search Google Scholar
    • Export Citation
  • Martino, N., Dell’Aquila, M., Cardone, R., Somoskoi, B., Lacalandra, G. and Cseh, S. (2013): Vitrification preserves chromatin integrity, bioenergy potential and oxidative parameters in mouse embryos.. Reprod. Biol. Endocrinol. 11, 2738.

    • Search Google Scholar
    • Export Citation
  • Moriwaki, T., Suganuma, N., Hayakawa, M., Hibi, H., Katsumata, Y., Oguchi, H. and Furuhashi, M. (2004): Embryo evaluation by analysing blastomere nuclei.. Hum. Reprod. 19, 152156.

    • Search Google Scholar
    • Export Citation
  • Nasri, T., Bosch, R., Voorde, S. and Fink-Gremmels, J. (2006): Differential induction of apoptosis by type A and B trichothecenes in Jurkat T-lymphocytes.. Toxicol. in Vitro 20, 832840.

    • Search Google Scholar
    • Export Citation
  • Saiz, N. and Plusa, B. (2013): Early cell fate decisions in the mouse embryos.. Reproduction 145, 6580.

  • Shapiro, B., Harris, D. and Richter, K. (2000): Predictive value of 72-hour blastomere cell number on blastocyst development and success of subsequent transfer based on the degree of blastocyst development.. Fertil. Steril. 73, 582586.

    • Search Google Scholar
    • Export Citation
  • Somoskoi, B., Keresztes, Z., Solti, L., Kovács, M. and Cseh, S. (2012): Effect of T-2 mycotoxin on early development of mouse embryos [in Hungarian, with English abstract].. Magy. Allatorvosok 10, 614619.

    • Search Google Scholar
    • Export Citation
  • Somoskoi, B., Kovács, M. and Cseh, S. (2014): Effects of T-2 mycotoxin on in vitro development and chromatin status of mouse embryos being in preimplantation stages. Toxicol. Ind. Health doi:10.1177/0748233714555394.

    • Search Google Scholar
    • Export Citation
  • Song, H., Han, K. and Lim, H. (2007): Progesterone supplementation extends uterine receptivity for blastocyst implantation in mice.. Reproduction 133, 487493.

    • Search Google Scholar
    • Export Citation
  • Tian, Y. and Yamanuchi, T. (2003): Micronucleus formation in 3-day mouse embryos associated with maternal exposure to chlorpyrifos during the early preimplantation period.. Repr. Tox. 17, 401405.

    • Search Google Scholar
    • Export Citation
  • Ványi, A., Glávits, R. and Molnár, T. (1995): Reproductive disorders due to F-2 and T-2 toxins in large-scale pig farms [in Hungarian, with English abstract].. Magy. Allatorvosok 50, 424430.

    • Search Google Scholar
    • Export Citation
  • Weidner, M., Hüwel, S., Ebert, F., Schwerdtle, T., Galla, H. and Humpf, H. (2013): Influence of T- 2 and HT-2 toxin on the blood-brain barrier in vitro: new experimental hints for neurotoxic effects.. PLoS One 8, e60484.

    • Search Google Scholar
    • Export Citation
  • Ye, X., Franco, A., Santos, H., Nelson, D., Kaufman, P. and Adams, P. (2003): Defective S phase chromatin assembly causes DNA damage, activation of the S phase checkpoint, and S phase arrest.. Mol. Cell 11, 341351.

    • Search Google Scholar
    • Export Citation
  • Yuan, G., Wang, Y., Yuan, X., Zhang, T., Zhao, J., Huang, L. and Peng, S. (2014): T-2 induces developmental toxicity and apoptosis in zebrafish embryos.. J. Environ. Sci. 26, 917925.

    • Search Google Scholar
    • Export Citation
  • Zakhartchenko, V., Wolf, E., Palma, G. and Brem, G. (1995): Effect of donor embryo cell number and cell size on the efficiency of bovine embryo cloning.. Mol. Reprod. Dev. 42, 5357.

    • Search Google Scholar
    • Export Citation

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 2 3
Jul 2020 4 0 3
Aug 2020 6 0 0
Sep 2020 4 0 0
Oct 2020 8 0 0
Nov 2020 7 1 0
Dec 2020 1 0 0