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  • 1 Kaposvár University, Guba S. u. 40, H-7400 Kaposvár, Hungary
  • 2 Hungarian Academy of Sciences — Kaposvár University ‘MTA-KE Mycotoxins in the Food Chain’ Research Group, Kaposvár, Hungary
  • 3 National Agricultural Research Centre, Herceghalom, Hungary
  • 4 Szent István University, Gödöllő, Hungary
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Adult male Wistar rats were enrolled in a study to test the acute hepatic effects of 50 mg/kg fumonisin B1 in feed for 5 days. Fumonisin B1 depressed growth and feed intake, and absolute and relative liver weight showed a significant increase. The proportions of C17:0, C18:3 n3, C22:5 n3 and C22:6 n3 fatty acids decreased in the hepatic phospholipid fraction. All proportional decreases modified the hepatocellular membrane lipids into a more rigid state. The fatty acid profile modifications were partly compensated for by endogenous glutathione (preventing the formation of conjugated dienes and trienes as initial phase lipid peroxidation indicators), while the enzymatic antioxidant defence system (glutathione peroxidase) was unaltered. In contrast, hepatic malondialdehyde, the cytotoxic product of end-phase lipid peroxidation showed a concentration increase even after 5 days of feeding. The results indicate a rather strong and rapid hepatic effect of FB1, immediately impairing membrane phospholipids, even before the enzymatic antioxidant defence is activated.

  • Abel, S. and Gelderblom, W. C. (1998): Oxidative damage and fumonisin B1-induced toxicity in primary rat hepatocytes and rat liver in vivo. Toxicology 131, 121131.

    • Search Google Scholar
    • Export Citation
  • Association of Official Analytical Chemists (1984): Official Methods of Analysis (28.054). 14th edition. Association of Official Analytical Chemists, Arlington, VA, USA.

    • Search Google Scholar
    • Export Citation
  • Bócsai, A., Pelyhe, C., Zándoki, E., Ancsin, Z., Szabó-Fodor, J., Erdélyi, M., Mézes, M. and Balogh, K. (2016): Short-term effects of T-2 toxin exposure on some lipid peroxide and glutathione redox parameters of broiler chickens. J. Anim. Physiol. Anim. Nutr. (Berl). 100, 520525.

    • Search Google Scholar
    • Export Citation
  • Christie, W. W. (1982): A simple procedure for rapid transmethylation of glycerolipids and cholesteryl esters. J. Lipid Res. 23, 10721075.

    • Search Google Scholar
    • Export Citation
  • Del Rio, D., Stewart, A. J. and Pellegrini, N. (2005): A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr. Metab. Cardiovasc. Dis. 15, 316328.

    • Search Google Scholar
    • Export Citation
  • Domijan, A., Zeljezic, D., Peraica, M., Kovacevic, G., Gregorovic, G., Krstanac, Z., Horvatin, K. and Kalafatic, M. (2008): Early toxic effects of fumonisin B1 in rat liver. Hum. Exp. Toxicol. 27, 895900.

    • Search Google Scholar
    • Export Citation
  • Dutton, M. F. (1996): Fumonisins, mycotoxins of increasing importance: their nature and their effects. Pharmacol. Ther. 70, 137161.

  • Fodor, J., Balogh, K., Weber, M., Mézes, M., Kametler, L., Pósa, R., Mamet, R., Bauer, J., Horn, P., Kovács, F. and Kovács, M. (2008): Absorption, distribution and elimination of fumonisin B(1) metabolites in weaned piglets. Food Addit. Contam. Part A 25, 8896.

    • Search Google Scholar
    • Export Citation
  • Folch, J., Lees, M. and Stanley, S. H. G. (1957): A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497509.

    • Search Google Scholar
    • Export Citation
  • Gelderblom, W. C., Abel, S., Smuts, C. M., Marnewick, J., Marasas, W. F., Lemmer, F. R. and Ramljak, D. (2001): Fumonisin-induced hepatocarcinogenesis: Mechanisms related to cancer initiation and promotion. Environ. Health Perspect. 109, Suppl. 2, 291300.

    • Search Google Scholar
    • Export Citation
  • Gelderblom, W. C., Cawood, M. E., Snyman, S. D. and Marasas, W. F. (1994): Fumonisin B1 dosimetry in relation to cancer initiation in rat liver. Carcinogenesis 15, 209214.

    • Search Google Scholar
    • Export Citation
  • Gelderblom, W. C., Smuts, C. M., Abel, S., Snyman, S., Van der Westhuizen, D. L., Huber, W. W. and Swanevelder, S. (1997): Effect of fumonisin B1 on the levels and fatty acid composition of selected lipids in rat liver in vivo. Food Chem. Toxicol. 35, 647656.

    • Search Google Scholar
    • Export Citation
  • Halliwell, B. and Cross, C. E. (1994): Oxygen-derived species: their relation to human disease and environmental stress. Environ. Health Perspect. 102, Suppl. 2, 512.

    • Search Google Scholar
    • Export Citation
  • Hulbert, A. J. (2005): On the importance of fatty acid composition of membranes for aging. J. Theor. Biol. 234, 277288.

  • IARC Working Group on the Evaluation of Carcinogenic Risks to Humans (2002): Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. IARC Monogr. Eval. Carcinog. Risks Hum. 82, 1556.

    • Search Google Scholar
    • Export Citation
  • Kang, Y. J. and Alexander, J. M. (1996): Alterations of the glutathione redox cycle status in fumonisin B1-treated pig kidney cells. J. Biochem. Toxicol. 11, 121126.

    • Search Google Scholar
    • Export Citation
  • Klaric, M. S., Pepeljnjak, S., Domijan, A. M. and Petrik, J. (2007): Lipid peroxidation and glutathione levels in porcine kidney PK15 cells after individual and combined treatment with fumonisin B(1), beauvericin and ochratoxin A. Basic Clin. Pharmacol. Toxicol. 100, 157164.

    • Search Google Scholar
    • Export Citation
  • Lawrence, R. A. and Burk, R. F. (1978): Species, tissue and subcellular distribution of non Sedependent glutathione peroxidase activity. J. Nutr. 108, 211215.

    • Search Google Scholar
    • Export Citation
  • Leray, C. (2013): Introduction to Lipidomics: From Bacteria to Man. CRC Press, Boca Raton, USA.

  • Leray, C., Andriamampandry, M., Gutbier, G., Cavadenti, J., Klein-Soyer, C., Gachet, C. and Cazenave, P. (1997): Quantitative analysis of vitamin E, cholesterol and phospholipid fatty acids in a single aliquot of human platelets and cultured endothelial cells. J. Chromatogr. B 696, 3342.

    • Search Google Scholar
    • Export Citation
  • Marnewick, J. L., van der Westhuizen, F. H., Joubert, E., Swanevelder S, Swart, P. and Gelderblom, W. C. (2009): Chemoprotective properties of rooibos (Aspalathus linearis), honeybush (Cyclopia intermedia) herbal and green and black (Camellia sinensis) teas against cancer promotion induced by fumonisin B1 in rat liver. Food Chem. Toxicol. 47, 220229.

    • Search Google Scholar
    • Export Citation
  • Mead, J. F., Alfin-Slater, R. B. and Howton, D. R. (1986): Lipids: Chemistry, Biochemistry and Nutrition. Plenum Press, N.Y., USA.

  • Placer, Z. A., Cushman, L. L. and Johnson, B. C. (1966): Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal. Biochem. 16, 359364.

    • Search Google Scholar
    • Export Citation
  • Pryor, W. A. and Stanley, J. P. (1975): Letter: A suggested mechanism for the production of malonaldehyde during the autoxidation of polyunsaturated fatty acids. Nonenzymatic production of prostaglandin endoperoxides during autoxidation. J. Org. Chem. 40, 36153617.

    • Search Google Scholar
    • Export Citation
  • Sahu, S. C., Eppley, R. M., Page, S. W., Gray, G. C., Barton, C. N. and O’Donnell, M. W. (1998): Peroxidation of membrane lipids and oxidative DNA damage by fumonisin B1 in isolated rat liver nuclei. Cancer Lett. 125, 117121.

    • Search Google Scholar
    • Export Citation
  • Sedlak, J. and Lindsay, R. H. (1968): Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal. Biochem. 25, 192205.

    • Search Google Scholar
    • Export Citation
  • Stockmann-Juvala, H., Mikkola, J., Naarala, J., Loikkanen, J., Elovaara, E. and Savolainen K. (2004): Oxidative stress induced by fumonisin B1 in continuous human and rodent neural cell cultures. Free Radic. Res. 38, 933942.

    • Search Google Scholar
    • Export Citation
  • Szabó, A., Szabó-Fodor, J., Fébel, H., Romvári, R. and Kovács, M. (2014): Individual and combined haematotoxic effects of fumonisin B(1) and T-2 mycotoxins in rabbits. Food Chem. Toxicol. 72, 257264.

    • Search Google Scholar
    • Export Citation
  • Szabó, A., Szabó-Fodor, J., Fébel, H., Mézes, M., Bajzik, G. and Kovács, M. (2016): Oral administration of fumonisin B1 and T-2 individually and in combination affects hepatic total and mitochondrial membrane lipid profile of rabbits. Acta Physiol. Hung. 103, 321333.

    • Search Google Scholar
    • Export Citation
  • Szabó-Fodor, J., Kachlek, M., Cseh, S., Somoskoi, B., Szabó, A., Blochné-Bodnár, Zs., Tornyos, G., Mézes, M., Balogh, K., Glávits, R., Hafner, D. and Kovács, M. (2015): Individual and combined effects of subchronic exposure of three Fusarium toxins (fumonisin B, deoxynivalenol and zearalenone) in rabbit bucks. J. Clin. Toxicol. 5, 264. doi:10.4172/2161-0495.1000264.

    • Search Google Scholar
    • Export Citation
  • Tirosh, O., Shpaizer, A. and Kanner, J. (2015): Lipid peroxidation in a stomach medium is affected by dietary oils (olive/fish) and antioxidants: The Mediterranean versus Western diet. J. Agric. Food Chem. 63, 70167023.

    • Search Google Scholar
    • Export Citation

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