View More View Less
  • 1 Cairo University, Giza, Egypt
  • 2 National Research Centre, Dokki, Giza, Egypt
  • 3 Cairo University, Giza, Egypt
  • 4 University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria
  • 5 National Institute of Infectious Diseases, Tokyo, Japan
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

Accurate and rapid identification of bacterial pathogens of fish is essential for the effective treatment and speedy control of infections. Massive mortalities in market-sized red tilapia (Oreochromis spp.) were noticed in mariculture concrete ponds in northern Egypt. Histopathological examination revealed marked congestion in the central vein of the liver with the presence of bacterial aggregates inside the lumen and in the vicinity of the central vein. A total of 12 isolates of streptococci were obtained from the moribund fish. This study documented the ability of the MicroSeq 500 16S bacterial sequencing method to accurately identify Streptococcus agalactiae and S. dysgalactiae mixed infections from moribund red tilapia that were difficult to be recognised by the commercial biochemical systems. The continuously decreasing cost of the sequencing technique should encourage its application in routine diagnostic procedures.

  • Abdelsalam, M., Asheg, A. and Eissa, A. E. (2013): Streptococcus dysgalactiae: an emerging pathogen of fishes and mammals. Int. J. Vet. Sci. Med. 1, 16.

    • Search Google Scholar
    • Export Citation
  • Abdelsalam, M., Chen, S-C. and Yoshida, T. (2009b): Surface properties of Streptococcus dysgalactiae strains isolated from marine fish. Bull. Eur. Assoc. Fish Pathol. 29, 1523.

    • Search Google Scholar
    • Export Citation
  • Abdelsalam, M., Chen, S-C. and Yoshida, T. (2010a): Phenotypic and genetic characterization of Streptococcus dysgalactiae strains isolated from fish collected in Japan and other Asian countries. FEMS Microbiol. Lett. 302, 3238.

    • Search Google Scholar
    • Export Citation
  • Abdelsalam, M., Chen, S-C. and Yoshida, T. (2010b): Dissemination of streptococcal pyrogenic exotoxin G (spegg) with an IS-like element in fish isolates of Streptococcus dysgalactiae. FEMS Microbiol. Lett. 309, 105113.

    • Search Google Scholar
    • Export Citation
  • Abdelsalam, M., Eissa, A. E. and Chen, S-C. (2015): Genetic diversity of geographically distinct Streptococcus dysgalactiae isolates from fish. J. Adv. Res. 6, 233238.

    • Search Google Scholar
    • Export Citation
  • Abdelsalam, M., Nakanishi, K., Yonemura, K., Itami, T., Chen, S-C. and Yoshida, T. (2009a): Application of Congo red agar for detection of Streptococcus dysgalactiae isolated from diseased fish. J. Appl. Ichthyol. 25, 442446.

    • Search Google Scholar
    • Export Citation
  • Abuseliana, A. F., Daud, H. H. M., Aziz, S. A., Bejo, S. K. and Alsaid, M. (2011): Pathogenicity of Streptococcus agalactiae isolated from a fish farm in Selangor to juvenile red tilapia (Oreochromis sp.). J. Anim. Vet. Adv. 10, 914919.

    • Search Google Scholar
    • Export Citation
  • Agius, C. and Roberts, R. J. (2003): Melano-macrophage centers and their role in fish pathology. J. Fish Dis. 26, 499509.

  • Amal, M. N. A. (2011): Prevalence, risk factors and transmission of Streptococcus agalactiae in the red hybrid tilapia (Oreochromis sp.). PhD Thesis, Faculty of Veterinary Medicine, University Putra Malaysia, Serdang. pp. 161184.

    • Search Google Scholar
    • Export Citation
  • Bancroft, J. D. and Gamble, M. (2008): Theory and Practice of Histological Techniques. 6th edition. Churchill Livingstone, Edinburgh.

  • Bardach, J. E., Ryther, J. H. and McLarney, W. O. (1972): Aquaculture: The Farming and Husbandry of Freshwater and Marine Organisms. John Wiley & Sons Inc., New York. 868 pp.

    • Search Google Scholar
    • Export Citation
  • Chang, P. H. and Plumb, J. A. (1996): Histopathology of experimental Streptococcus sp.infection in tilapia, Oreochromis niloticus, and channel catfish, Ictalurus punctatus. J. Fish Dis. 19, 235241.

    • Search Google Scholar
    • Export Citation
  • Chen, C., Chao, C. and Bowser, P. R. (2007): Comparative histopathology of Streptococcus iniae and Streptococcus agalactiae-infected tilapia. Bull. Eur. Assoc. Fish Pathol. 27, 29.

    • Search Google Scholar
    • Export Citation
  • Elgendy, M. Y. (2013): Epizootiological and molecular studies on the common septicemic bacterial diseases of some saltwater fishes. PhD Thesis, Cairo University, Egypt.

    • Search Google Scholar
    • Export Citation
  • Eltholth, M., Fornace, K., Grace, D., Rushton, J. and Hasler, B. (2015): Characterisation of production, marketing and consumption patterns of farmed tilapia in the Nile Delta of Egypt. Food Policy 51, 131143.

    • Search Google Scholar
    • Export Citation
  • Evans, J. J., Klesius, P. H., Gilbert, P. M., Shoemaker, C. A., Al Sarawi, M. A., Landsberg, J., Duremdez, R., Al Marzouk, A. and Al Zenki, S. (2002): Characterization of ß-haemolytic Group B Streptococcus agalactiae in cultured seabream, Sparus auratus L., and wild mullet, Liza klunzingeri (Day), in Kuwait. J. Fish Dis. 25, 505513.

    • Search Google Scholar
    • Export Citation
  • Fawzy, N. M., Osman, K. M., Ibrahim, M., Naguib, M., Ali, M. and Abd-Elrahman, S. (2014): Streptococcosis in tilapia: Clinico-pathological picture of experimentally infected tilapia. Life Sci. J. 11, 10051012.

    • Search Google Scholar
    • Export Citation
  • Hernandez, E., Figueroa, J. and Iregui, C. (2009): Streptococcosis on a red tilapia, Oreochromis sp. farm: A case study. J. Fish Dis. 32, 247252.

    • Search Google Scholar
    • Export Citation
  • Hopkins, M. J., Sharp, R. and Macfarlane, G. T. (2001): Age and disease related changes in intestinal bacterial populations assessed by cell culture, 16S rRNA abundance, and community cellular fatty acid profiles. Gut 48, 198205.

    • Search Google Scholar
    • Export Citation
  • Hulata, G., Karplus, I. and Harpaz, S. (1995): Evaluation of some red tilapia strains for aquaculture: Growth and color segregation in hybrid progeny. Aquac. Res. 26, 765771.

    • Search Google Scholar
    • Export Citation
  • Lahav, D., Eyngor, M., Hurvitz, A., Ghittino, C., Lublin, A. and Eldar, A. (2004): Streptococcus iniae type II infections in rainbow trout Oncorhynchus mykiss. Dis. Aquat. Org. 62, 177180.

    • Search Google Scholar
    • Export Citation
  • Moustafa, M., Eissa, A. E., Laila, A. M., Gaafar, A. Y., Abumourad, I. M. and Elgendy, M. Y. (2015): Investigations into the potential causes of mass kills in mari-cultured gilthead seabream (Sparus aurata) at Northern Egypt. Pharm. Biol. Chem. Sci. 6, 466477.

    • Search Google Scholar
    • Export Citation
  • Nomoto, R., Munasinghe, L. I., Jin, D. H., Shimahara, Y., Yasuda, H., Nakamura, A., Misawa, N., Itami, T. and Yoshida, T. (2004): Lancefield group C Streptococcus dysgalactiae infection responsible for fish mortalities in Japan. J. Fish Dis. 27, 679686.

    • Search Google Scholar
    • Export Citation
  • Pretto-Giordano, L. G., Müller, E. E., Klesius, P. and Silva, V. G. D. (2010): Efficacy of an experimentally inactivated Streptococcus agalactiae vaccine in Nile tilapia (Oreochromis niloticus) reared in Brazil. Aquac. Res. 14, 15391544.

    • Search Google Scholar
    • Export Citation
  • Romana-Eguia, M. R. and Eguia, R. V. (1999): Growth of five Asian red tilapia strains in saline environments. Aquaculture 173, 161170.

    • Search Google Scholar
    • Export Citation
  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011): MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 27312739.

    • Search Google Scholar
    • Export Citation
  • Verner-Jeffreys, D., Baker-Austin, C., Pond, M., Rimmer, G., Kerr, R. and Stone, D. (2012): Zoonotic disease pathogens in fish used for pedicure. Emerg. Infect. Dis. 18, 10061008.

    • Search Google Scholar
    • Export Citation
  • Wagner, R., Paine, D. and Cerniglia, C. (2003): Phenotypic and genotypic characterization of competitive exclusion products for use in poultry. J. Appl. Microbiol. 94, 10981107.

    • Search Google Scholar
    • Export Citation
  • Watts, J. L. and Yancey, R. J. J. (1994): Identification of veterinary pathogens by use of commercial identification systems and new trends in antimicrobial susceptibility testing of veterinary pathogens. Clin. Microbiol. Rev. 7, 346356.

    • Search Google Scholar
    • Export Citation
  • Woo, P. C., Teng, J. L., Wu, J. K., Leung, F. P., Tse, H., Fung, A., Lau, S. and Yuen, K. (2009): Guidelines for interpretation of 16S rRNA gene sequence-based results for identification of medically important aerobic Gram-positive bacteria. J. Med. Microbiol. 58, 10301036.

    • Search Google Scholar
    • Export Citation
  • Zamri-Saad, M., Amal, M. and Siti-Zahrah, A. (2010): Pathological changes in red tilapias (Oreochromis spp.) naturally infected by Streptococcus agalactiae. J. Comp. Pathol. 143, 227229.

    • Search Google Scholar
    • Export Citation

The author instruction is available in PDF.
Please, download the file from HERE.

 

MANUSCRIPT SUBMISSION

  • Impact Factor (2019): 0.991
  • Scimago Journal Rank (2019): 0.372
  • SJR Hirsch-Index (2019): 32
  • SJR Quartile Score (2019): Q2 Veterinary (miscellaneous)
  • Impact Factor (2018): 1.059
  • Scimago Journal Rank (2018): 0.517
  • SJR Hirsch-Index (2018): 30
  • SJR Quartile Score (2018): Q2 Veterinary (miscellaneous)

Language: English

Founded in 1951
Publication: One volume of four issues annually
Publication Programme: 2020. Vol. 68.
Indexing and Abstracting Services:

  • Biological Abstracts

     

  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture, Biology and Environmental Sciences
  • Elsevier Science Navigator
  • Focus On: Veterinary Science and Medicine
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Subscribers can access the electronic version of every printed article.

Senior editors

Editor(s)-in-Chief: Benkő, Mária

Managing Editor(s): Székely, András

Editorial Board

      Dénes, Béla
      Eszterbauer, Edit
      Fébel, Hedvig
      Fodor, László
      Harrach, Balázs
      Andras Komaromy (USA)
      Peter Massanyi (Slovak Republic)
      Nagy, Béla
      Németh, Tibor
      Neogrády, Zsuzsanna
      Kurt Pfister (Germany)
      Solti, László
      Szabó, József
      Vajdovich, Péter
      Varga, János
      Štefan, Vilĉek (Slovak Republic)
      Vörös, Károly
      Herbert Weissenböck (Austria)
      Zsarnovszky, Attila

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 467 4081 (ed.-in-chief) or (36 1) 213 9793 (editor) Fax: (36 1) 467 4076 (ed.-in-chief) or (36 1) 213 9793