View More View Less
  • 1 University of Veterinary Medicine, Hungária krt. 23–25, H-1143 Budapest, Hungary
  • 2 Hungarian Academy of Sciences, Budapest, Hungary
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

A total of 860 serum samples collected at 86 cattle farms in different parts of Hungary were screened for the presence of antibodies to Mycoplasma bovis using an ELISA test with a recombinant M. bovis membrane protein as antigen. Antibodies to M. bovis were detected in sera collected on all farms, and no farms negative for M. bovis were found. In 88.38% of the herds more than 50% of the sampled animals were infected by M. bovis. A total of 82.91% of the animals had antibodies to M. bovis. The proportion of seropositive animals was higher in the older age groups, and a significant difference was seen in the level of seropositivity between young and older age groups. The results show that M. bovis infection is widespread on Hungarian dairy farms, and its prevalence has increased in the recent decade. The high infection rate of Hungarian cattle herds with M. bovis shows that special attention should be paid to evaluating the aetiological role of M. bovis in bovine respiratory disease complex (BRDC) cases because M. bovis has an immunosuppressive effect and can predispose cattle to other respiratory infections, too.

  • Adamu, J. Y., Wawegama, N. K., Browning, G. F. and Markham, P. F. (2013): Membrane proteins of Mycoplasma bovis and their role in the pathogenesis. Res. Vet. Sci. 95, 321325.

    • Search Google Scholar
    • Export Citation
  • Arcangioli, M. A., Duet, A., Meyer, G., Dernburg, A., Bézille, P., Poumarat, F. and La Grand, D. (2008): The role of Mycoplasma bovis in bovine respiratory disease outbreaks in veal calf feedlots. Vet. J. 177, 8993.

    • Search Google Scholar
    • Export Citation
  • Arcangioli, M. A., Froux, W., Poumarat, F. and Le Grand, D. (2012): Case reports: Mycoplasma bovis otitis in French dairy calves. Rev. Med. Vet. 163, 284286.

    • Search Google Scholar
    • Export Citation
  • Bashiruddin, J. B., de Santis, P., Varga, E. and Stipkovits, L. (2001): Confirmation of the presence of Mycoplasma bovis in Hungarian cattle with pneumonia resembling pleuropneumonia. Vet. Rec. 148, 743746.

    • Search Google Scholar
    • Export Citation
  • Bürki, S., Frey, J. and Pilo, P. (2015): Virulence, persistence and dissemination of Mycoplasma bovis. Vet. Microbiol. 179, 1522.

  • Cusack, P. M. V., McMeniman, N. and Lean, I. J. (2003): The medicine and epidemiology of bovine respiratory disease in feedlots. Aust. Vet. J. 81, 480487.

    • Search Google Scholar
    • Export Citation
  • Fu, P., Sun, Z., Zhang, Y., Yu, Z., Zhang, H., Su, D., Jiang, F. and Wu, W. (2014): Development of a direct competitive ELISA for the detection of Mycoplasma bovis infection based on a monoclonal antibody of P48 protein. BMC Vet. Res. 10, 42.

    • Search Google Scholar
    • Export Citation
  • Gabinaitiene, A., Siugzdaite, J. and Zilinskas, H. (2011): Laboratory diagnosis of Mycoplasma infection in young cattle. Pol. J. Vet. Sci. 14, 8793.

    • Search Google Scholar
    • Export Citation
  • Gagea, M. I., Bateman, K. G., van Dreumel, T., McEwen, B. J., Carman, S., Archambault, M., Shanahan, R. A. and Caswell, J. L. (2006 a): Diseases and pathogens associated with mortality in Ontario beef feedlots. J. Vet. Diagn. Invest. 18, 1828.

    • Search Google Scholar
    • Export Citation
  • Gagea, M. I., Bateman, K. G., Shanahan, R. A., van Dreumel, T., McEwen, B. J., Carman, S., Archambault, M. and Caswell, J. L. (2006 b): Naturally occurring Mycoplasma bovis-associated pneumonia and polyarthritis in feedlot beef calves. J. Vet. Diagn. Invest. 18, 2940.

    • Search Google Scholar
    • Export Citation
  • Giovannini, S., Zanoni, M. G., Salogni, C., Cinotti, S. and Alborali, G. L. (2013): Mycoplasma bovis infection in respiratory disease of dairy calves less than one month old. Res. Vet. Sci. 95, 576579.

    • Search Google Scholar
    • Export Citation
  • Gulliksen, S. M., Jor, E., Lie, K. I., Loken, T., Akerstedt, J. and Osteras, O. (2009): Respiratory infections in Norwegian dairy calves. J. Dairy Sci. 92, 51395146.

    • Search Google Scholar
    • Export Citation
  • Han, X., Khan, F. A., Zhu, X., Zhang, R., Mustafa, R., Hu, C., Chen, Y., Chen, H. and Guo, A. (2015): Establishment of an antibody avidity test to differentiate vaccinated cattle from those naturally infected with Mycoplasma bovis. Vet. J. 203, 7984.

    • Search Google Scholar
    • Export Citation
  • Kirby, F. D. and Nicholas, R. A. J. (1996): Isolation of Mycoplasma bovis from bullock’s eyes. Vet. Rec. 138, 552.

  • Nicholas, R. A. J. and Ayling, R. D. (2003): Mycoplasma bovis: disease, diagnosis, control. Res. Vet. Sci. 74, 105112.

  • Radaelli, E., Luini, M., Loria, G. R., Nicholas, R. A. J. and Scanziani, E. (2008): Bacteriological, serological, pathological and immunohistochemical studies of Mycoplasma bovis respiratory infection in veal calves and adult cattle at slaughter. Res. Vet. Sci. 85, 282290.

    • Search Google Scholar
    • Export Citation
  • Romváry, J., Rózsa, J., Stipkovits, L. and Mészáros, J. (1977): Pneumonia-arthritis syndrome in calves caused by Mycoplasma bovis (M. agalactiae subsp. bovis) [in Hungarian, with English abstract]. Magy. Allatorvosok 32, 801806.

    • Search Google Scholar
    • Export Citation
  • Siugzdaite, J., Gabinaitiene, A. and Kerziene, S. (2012): Susceptibility of Mycoplasma bovis field isolates to antimicrobial agents. Vet. Med. (Praha) 57, 575582.

    • Search Google Scholar
    • Export Citation
  • Soehnlen, M. K., Aydin, A., Murthy, K. S., Lengerich, E. J., Hattel, A. L., Houser, B. A., Fenton, G. D., Lysczek, H. R., Burns, C. M., Townsend, A. M., Brooks, J. W., Wolfgang, D. R. and Jayarao, B. M. (2012): Epidemiology of Mycoplasma bovis in Pennsylvania veal calves. J. Dairy Sci. 95, 247254.

    • Search Google Scholar
    • Export Citation
  • Stipkovits, L., Ripley, P., Varga, J. and Pálfi, V. (2000): Clinical study of the disease of calves associated with Mycoplasma bovis infection. Acta Vet. Hung. 48, 387395.

    • Search Google Scholar
    • Export Citation
  • Sulyok, K. M., Kreizinger, Z., Fekete, L., Jánosi, S., Schweitzer, N., Turcsányi, I., Makrai, L., Erdélyi, K. and Gyuranecz, M. (2014): Phylogeny of Mycoplasma bovis isolates from Hungary based on multilocus sequence typing and multiple-locus variable-number tandem repeat analysis. BMC Vet. Res. 10, 108.

    • Search Google Scholar
    • Export Citation
  • Tenk, M., Bálint, Á., Stipkovits, L., Bíró, J. and Dencso, L. (2006): Detection of Mycoplasma bovis with an improved PCR assay. Acta Vet. Hung. 54, 427435.

    • Search Google Scholar
    • Export Citation
  • Tenk, M., Ball, H. and Stipkovits, L. (2002): Rapid diagnosis of Mycoplasma bovis infection in cattle by capture ELISA test and selective differentiating medium [in Hungarian, with English abstract]. Magyar Allatorvosok 124, 333336.

    • Search Google Scholar
    • Export Citation
  • Tenk, M., Stipkovits, L. and Hufnagel, L. (2004): Examination of the role of Mycoplasma bovis in bovine pneumonia and a mathematical model for its evaluation. Acta Vet. Hung. 52, 445456.

    • Search Google Scholar
    • Export Citation
  • Wiggins, M. C., Woolums, A. R., Sanches, S., Hurley, D. J., Cole, D. J., Ensley, D. T. and Pence, M. E. (2007): Prevalence of Mycoplasma bovis in backgrounding and stocker cattle operations. J. Am. Vet. Med. Assoc. 230, 15141518.

    • Search Google Scholar
    • Export Citation