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  • 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China
  • | 2 Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, P. R. China
  • | 3 College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, P. R. China
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Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes are three bacterial pathogens closely associated with the bovine respiratory disease complex (BRDC). In the current study, a multiplex PCR for the simultaneous detection of these three bacteria in cultures was established. After serial optimisation, the detection limit of the method for the genomic DNA of the three bacteria was 40 pg/μl. The method could detect the genomic DNA of these three bacteria but not the genomic DNA of seven other bacterial strains. Together with the bacterial enrichment technology, the multiplex PCR could be used for detecting the three bacteria in animal tissues. This method might be valuable for speeding up laboratory diagnosis and directing the treatment of BRDC to these three bacterial pathogens.

  • Aghamiri, S. M., Haghkhah, M., Ahmadi, M. R. and Gheisari, H. R. (2014): Development of a multiplex PCR for the identification of major pathogenic bacteria of post-partum endometritis in dairy cows. Reprod. Domest. Anim. 49, 233238.

    • Search Google Scholar
    • Export Citation
  • Angen, O., Thomsen, J., Larsen, L. E., Larsen, J., Kokotovic, B., Heegaard, P. M. and Enemark, J. M. (2009): Respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response. Vet. Microbiol. 137, 165171.

    • Search Google Scholar
    • Export Citation
  • Billington, S. J., Jost, B. H., Cuevas, W. A., Bright, K. R. and Songer, J. G. (1997): The Arcanobacterium (Actinomyces) pyogenes hemolysin, pyolysin, is a novel member of the thiolactivated cytolysin family. J. Bacteriol. 179, 61006106.

    • Search Google Scholar
    • Export Citation
  • Confer, A. W. (2009): Update on bacterial pathogenesis in BRD. Anim. Health Res. Rev. 10, 145148.

  • Dagleish, M. P., Bayne, C. W., Moon, G. G., Finlayson, J., Sales, J., Williams, J. and Hodgson, J. C. (2016): Differences in virulence between bovine-derived clinical isolates of Pasteurella multocida serotype A from the UK and the USA in a model of bovine pneumonic pasteurellosis. J. Comp. Pathol. 155, 6271.

    • Search Google Scholar
    • Export Citation
  • Elbrissi, A., Sabeil, Y. A., Khalifa, K. A., Enan, K., Khair, O. M. and El Hussein, A. M. (2017): Isolation, identification and differentiation of Campylobacter spp. using multiplex PCR assay from goats in Khartoum State, Sudan. Trop. Anim. Health Prod. 49, 575581.

    • Search Google Scholar
    • Export Citation
  • Feng, K., Hu, W., Jiang, A., Sarengaowa Xu, Y., Zou, Y., Yang, L. and Wang, X. (2016): A dual filtration-based multiplex PCR method for simultaneous detection of viable Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus on fresh-cut cantaloupe. PloS One 11, e0166874.

    • Search Google Scholar
    • Export Citation
  • Griffin, D. (2010): Bovine pasteurellosis and other bacterial infections of the respiratory tract. Vet. Clinics North Am. — Food Anim. Pract. 26, 5771.

    • Search Google Scholar
    • Export Citation
  • Grissett, G. P., White, B. J. and Larson, R. L. (2015): Structured literature review of responses of cattle to viral and bacterial pathogens causing bovine respiratory disease complex. J. Vet. Intern. Med. 29, 770780.

    • Search Google Scholar
    • Export Citation
  • Horwood, P. F. and Mahony, T. J. (2011): Multiplex real-time RT-PCR detection of three viruses associated with the bovine respiratory disease complex. J. Virol. Methods 171, 360363.

    • Search Google Scholar
    • Export Citation
  • Hu, Q., Tu, J., Han, X., Zhu, Y., Ding, C. and Yu, S. (2011): Development of multiplex PCR assay for rapid detection of Riemerella anatipestifer, Escherichia coli, and Salmonella enterica simultaneously from ducks. J. Microbiol. Methods 87, 6469.

    • Search Google Scholar
    • Export Citation
  • Kardos, G., Nagy, J., Antal, M., Bistyák, A., Tenk, M. and Kiss, I. (2007): Development of a novel PCR assay specific for Riemerella anatipestifer. Lett. Appl. Microbiol. 44, 145148.

    • Search Google Scholar
    • Export Citation
  • Kishimoto, M., Tsuchiaka, S., Rahpaya, S. S., Hasebe, A., Otsu, K., Sugimura, S., Kobayashi, S., Komatsu, N., Nagai, M., Omatsu, T., Naoi, Y., Sano, K., Okazaki-Terashima, S., Oba, M., Katayama, Y., Sato, R., Asai, T. and Mizutani, T. (2017): Development of a one-run realtime PCR detection system for pathogens associated with bovine respiratory disease complex. J. Vet. Med. Sci. 79, 517523.

    • Search Google Scholar
    • Export Citation
  • Lima, S. F., Teixeira, A. G., Higgins, C. H., Lima, F. S. and Bicalho, R. C. (2016): The upper respiratory tract microbiome and its potential role in bovine respiratory disease and otitis media. Sci. Rep. 6, 29050. doi: 10.1038/srep29050.

    • Search Google Scholar
    • Export Citation
  • Lubbers, B. V. and Turnidge, J. (2015): Antimicrobial susceptibility testing for bovine respiratory disease: getting more from diagnostic results. Vet. J. 203, 149154.

    • Search Google Scholar
    • Export Citation
  • Moriconi, M., Acke, E., Petrelli, D. and Preziuso, S. (2017): Multiplex PCR-based identification of Streptococcus canis, Streptococcus zooepidemicus and Streptococcus dysgalactiae subspecies from dogs. Comp. Immunol. Microbiol. Infect. Dis. 50, 4853.

    • Search Google Scholar
    • Export Citation
  • Nattermann, H. and Horsch, F. (1977): The Corynebacterium pyogenes infection in cattle. 1. Incidence of the pathogen. Arch. Exp. Vet.-Med. 31, 405413.

    • Search Google Scholar
    • Export Citation
  • Panciera, R. J. and Confer, A. W. (2010): Pathogenesis and pathology of bovine pneumonia. Vet. Clin. North Am. — Food Anim. Pract. 26, 191214.

    • Search Google Scholar
    • Export Citation
  • Shoo, M. K., Wiseman, A., Allan, E. M., Dalgleish, R. G., Gibbs, H.A., Al-Hendi, A. B. and Selman, I. E. (1990): Distribution of Pasteurella haemolytica in the respiratory tracts of carrier calves and those subsequently infected experimentally with Dictyocaulus viviparus. Res. Vet. Sci. 48, 383385.

    • Search Google Scholar
    • Export Citation
  • Thonur, L., Maley, M., Gilray, J., Crook, T., Laming, E., Turnbull, D., Nath, M. and Willoughby, K. (2012): One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3. BMC Vet. Res. 8, 37.

    • Search Google Scholar
    • Export Citation
  • Townsend, K. M., Frost, A. J., Lee, C. W., Papadimitriou, J. M. and Dawkins, H. J. (1998): Development of PCR assays for species- and type-specific identification of Pasteurella multocida isolates. J. Clin. Microbiol. 36, 10961100.

    • Search Google Scholar
    • Export Citation

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Senior editors

Editor-in-Chief: Mária BENKŐ

Managing Editor: András SZÉKELY

Editorial Board

  • Béla DÉNES (National Food Chain Safety Office, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (National Agricultural Innovation Centre, Herceghalom, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA
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Phone: (36 1) 467 4081 (ed.-in-chief) or (36 1) 213 9793 (editor) Fax: (36 1) 467 4076 (ed.-in-chief) or (36 1) 213 9793

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2020  
Total Cites 987
WoS
Journal
Impact Factor
0,955
Rank by Veterinary Sciences 101/146 (Q3)
Impact Factor  
Impact Factor 0,920
without
Journal Self Cites
5 Year 1,164
Impact Factor
Journal  0,57
Citation Indicator  
Rank by Journal  Veterinary Sciences 93/166 (Q3)
Citation Indicator   
Citable 49
Items
Total 49
Articles
Total 0
Reviews
Scimago 33
H-index
Scimago 0,395
Journal Rank
Scimago Veterinary (miscellaneous) Q2
Quartile Score  
Scopus 355/217=1,6
Scite Score  
Scopus General Veterinary 73/183 (Q2)
Scite Score Rank  
Scopus 0,565
SNIP  
Days from  145
submission  
to acceptance  
Days from  150
acceptance  
to publication  
Acceptance 19%
Rate

 

2019  
Total Cites
WoS
798
Impact Factor 0,991
Impact Factor
without
Journal Self Cites
0,897
5 Year
Impact Factor
1,092
Immediacy
Index
0,119
Citable
Items
59
Total
Articles
59
Total
Reviews
0
Cited
Half-Life
9,1
Citing
Half-Life
9,2
Eigenfactor
Score
0,00080
Article Influence
Score
0,253
% Articles
in
Citable Items
100,00
Normalized
Eigenfactor
0,09791
Average
IF
Percentile
42,606
Scimago
H-index
32
Scimago
Journal Rank
0,372
Scopus
Scite Score
335/213=1,6
Scopus
Scite Score Rank
General Veterinary 62/178 (Q2)
Scopus
SNIP
0,634
Acceptance
Rate
18%

 

Acta Veterinaria Hungarica
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Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation
1951
Publication
Programme
2020 Volume 68
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

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