The Polled Intersex Syndrome (PIS) is responsible for the absence of horns in homozygous and heterozygous goats causing a female-to-male sex reversal in the homozygous polled genotypic female (XX) goats. A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. The PCR products were easily distinguishable using agarose gel electrophoresis: we detected an X-specific single band in samples originating from healthy phenotypic females and double (X- and Y-) bands in samples from males. The new PCR method is applicable for diagnosing the sex of PIS-affected animals already as newborn kids, in contrast with the phenotypic findings appearing only after puberty, and thus it may replace the cumbersome chromosome investigations.
Altschul, S., Gish, W., Miller, W., Myers, E. and Lipman, D. (1990): Basic local alignment search tool. J. Mol. Biol. 215, 403–410.
A-qin, C., Zi-rong, X. and Song-dong, Y. (2007): Sexing goat embryos by PCR amplification of Xand Y-chromosome specific sequence of the amelogenin gene. Asian-Aust. J. Anim. Sci. 20, 1689–1693.
Asdell, S. A. (1944): The genetic sex of intersexual goats and probable linkage with the gene for hornlessness. Science 99, 124.
Basrur, P. K. and Kanagawa, H. (1969): Anatomic and cytogenetic studies on 19 hornless goats with sexual disorders. Ann. Génét. Sél. Anim. 1, 349–378.
Ennis, S. and Gallagher, T. F. (1994): A PCR-based sex determination assay in cattle based on the bovine amelogenin locus. Anim. Genet. 25, 425–427.
Laurent, B., Maelle, P., Laurence, G., Aurelie, A. B., Maeva, E., Daniel, L. B., Nathalie, D., Christophe, R., Corinne, C., Norbert, B. G. and Eric, P. (2014): FOXL2 is a female sexdetermining gene in the goat. Current Biolog. 4, 404–408.
Maudet, C., Luikart, G., Dubray, D., Von Hardenberg, A. and Taberlet, P. (2004): Low genotyping error rates in wild ungulate feces sampled in winter. Mol. Ecol. Notes 4, 772–775.
Murphy, M. A., Waits, L. P. and Kendall, K. C. (2003): Influence of diet on faecal DNA amplification and sex identification in brown bears (Ursus arctos). Mol. Ecol. 12, 2261–2265.
Pourjafar, M., Badiei, K., Sharifiyazdi, H., Naghib, M. S., Chalmeh, A. and Divar, M. R. (2012): Application of hormonal and single multiplex PCR assays for detection of freemartinism in a horned goat. J. Fac. Vet. Med. 38, 175–181.
Schmidt, D., Ovitt, C. E., Anlag, K., Fehsenfeld, S., Gredsted, L., Treier, A. C. and Treier, M. (2004): The murine winged-helix transcription factor Foxl2 is required for granulosa cell differentiation and ovary maintenance. Development 131, 933–942.
Uhlenhaut, N. H. and Treier, M. (2006): Foxl2 function in ovarian development. Mol. Genet. Metab. 88, 225–234.
Waits, L. P. and Paetkau, D. (2005): Non-invasive genetic sampling of wildlife. J. Wildl. Manage. 69, 1419–1433.
Weikard, R., Pitra, C. and Kühn, C. (2006): Amelogenin crossamplification in the family bovidae and its application for sex determination. Mol. Reprod. Dev. 73, 1333–1337.