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  • 1 National Agricultural Research and Innovation Centre, Szent-Györgyi Albert u. 4, H-2100 Gödöllő, Hungary
  • 2 Szent István University, Gödöllő, Hungary
  • 3 University of Debrecen, Debrecen, Hungary
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The Polled Intersex Syndrome (PIS) is responsible for the absence of horns in homozygous and heterozygous goats causing a female-to-male sex reversal in the homozygous polled genotypic female (XX) goats. A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. The PCR products were easily distinguishable using agarose gel electrophoresis: we detected an X-specific single band in samples originating from healthy phenotypic females and double (X- and Y-) bands in samples from males. The new PCR method is applicable for diagnosing the sex of PIS-affected animals already as newborn kids, in contrast with the phenotypic findings appearing only after puberty, and thus it may replace the cumbersome chromosome investigations.

  • Altschul, S., Gish, W., Miller, W., Myers, E. and Lipman, D. (1990): Basic local alignment search tool. J. Mol. Biol. 215, 403410.

  • A-qin, C., Zi-rong, X. and Song-dong, Y. (2007): Sexing goat embryos by PCR amplification of Xand Y-chromosome specific sequence of the amelogenin gene. Asian-Aust. J. Anim. Sci. 20, 16891693.

    • Search Google Scholar
    • Export Citation
  • Asdell, S. A. (1944): The genetic sex of intersexual goats and probable linkage with the gene for hornlessness. Science 99, 124.

  • Basrur, P. K. and Kanagawa, H. (1969): Anatomic and cytogenetic studies on 19 hornless goats with sexual disorders. Ann. Génét. Sél. Anim. 1, 349378.

    • Search Google Scholar
    • Export Citation
  • Ennis, S. and Gallagher, T. F. (1994): A PCR-based sex determination assay in cattle based on the bovine amelogenin locus. Anim. Genet. 25, 425427.

    • Search Google Scholar
    • Export Citation
  • Laurent, B., Maelle, P., Laurence, G., Aurelie, A. B., Maeva, E., Daniel, L. B., Nathalie, D., Christophe, R., Corinne, C., Norbert, B. G. and Eric, P. (2014): FOXL2 is a female sexdetermining gene in the goat. Current Biolog. 4, 404408.

    • Search Google Scholar
    • Export Citation
  • Maudet, C., Luikart, G., Dubray, D., Von Hardenberg, A. and Taberlet, P. (2004): Low genotyping error rates in wild ungulate feces sampled in winter. Mol. Ecol. Notes 4, 772775.

    • Search Google Scholar
    • Export Citation
  • Murphy, M. A., Waits, L. P. and Kendall, K. C. (2003): Influence of diet on faecal DNA amplification and sex identification in brown bears (Ursus arctos). Mol. Ecol. 12, 22612265.

    • Search Google Scholar
    • Export Citation
  • Pourjafar, M., Badiei, K., Sharifiyazdi, H., Naghib, M. S., Chalmeh, A. and Divar, M. R. (2012): Application of hormonal and single multiplex PCR assays for detection of freemartinism in a horned goat. J. Fac. Vet. Med. 38, 175181.

    • Search Google Scholar
    • Export Citation
  • Schmidt, D., Ovitt, C. E., Anlag, K., Fehsenfeld, S., Gredsted, L., Treier, A. C. and Treier, M. (2004): The murine winged-helix transcription factor Foxl2 is required for granulosa cell differentiation and ovary maintenance. Development 131, 933942.

    • Search Google Scholar
    • Export Citation
  • Uhlenhaut, N. H. and Treier, M. (2006): Foxl2 function in ovarian development. Mol. Genet. Metab. 88, 225234.

  • Waits, L. P. and Paetkau, D. (2005): Non-invasive genetic sampling of wildlife. J. Wildl. Manage. 69, 14191433.

  • Weikard, R., Pitra, C. and Kühn, C. (2006): Amelogenin crossamplification in the family bovidae and its application for sex determination. Mol. Reprod. Dev. 73, 13331337.

    • Search Google Scholar
    • Export Citation

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