Rapid in-house detection method of Campylobacter spp. from food by redox potential monitoring combined with real-time PCR

Orsolya Erdősi; Katalin Szakmár; Zsuzsanna Szili; Géza Szita; Sándor Bernáth; József Sövényi; Péter Laczay

doi:10.1556/004.2018.001

Acta Veterinaria Hungarica

Volume/Issue:: Volume 66: Issue 1

Rapid in-house detection method of Campylobacter spp. from food by redox potential monitoring combined with real-time PCR

Authors:

Orsolya Erdősi

Orsolya Erdősi Department of Food Hygiene, University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary

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Katalin Szakmár

Katalin Szakmár Department of Food Hygiene, University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary

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Zsuzsanna Szili

Zsuzsanna Szili Department of Food Hygiene, University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary

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Géza Szita

Géza Szita Department of Food Hygiene, University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary

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Sándor Bernáth

Sándor Bernáth National Food Chain Safety Office, Directorate of Veterinary Medicinal Products, Budapest, Hungary

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József Sövényi

József Sövényi Sooke Veterinary Hospital Ltd., Sooke, B.C., Canada

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, and

Péter Laczay

Péter Laczay Department of Food Hygiene, University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary

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Pages:: 1–11

Online Publication Date:: Mar 2018

Publication Date:: 01 Mar 2018

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/004.2018.001

Keywords:: Rapid detection method; redox potential; food safety; raw milk; broiler meat

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The rapid detection of Campylobacter spp. is of utmost importance for the reduction of infections in humans by contaminated food products. The standard culturing method (ISO 10272-1:2006) involves a high time and labour demand. In this paper, we present a method that reduces the detection time of Campylobacter spp. to or below one third as compared to the ISO method, at a reduced cost per test. We used redox potential change of enrichment cultures (Bolton broth with Bolton selective supplement) for reliably selecting Campylobacter-contaminated raw milk and broiler meat samples. Identification of Campylobacter spp. in the contaminated samples was done by real-time PCR method. Culturing time to conclusive redox monitoring varied between 6 and 24 h for positive samples, depending on the contamination rate, in contrast to 136 h with the standard culturing process. However, now the Campylobacter-negative majority of food samples will not need to be tested by real-time PCR because redox potential monitoring can identify them in the selective enrichment phase. This method could be potentially used as a faster alternative to the current standard ISO 10272-1:2006, for nonregulatory monitoring purposes.

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Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

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Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language	English
Size	A4
Year of Foundation	1951
Volumes per Year	1
Issues per Year	4
Founder	Magyar Tudományos Akadémia
Founder's Address	H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher	Akadémiai Kiadó
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ISSN	0236-6290 (Print)
ISSN	1588-2705 (Online)