View More View Less
  • 1 University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary
  • | 2 Directorate of Veterinary Medicinal Products, Budapest, Hungary
  • | 3 Sooke Veterinary Hospital Ltd., Sooke, B.C., Canada
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

The rapid detection of Campylobacter spp. is of utmost importance for the reduction of infections in humans by contaminated food products. The standard culturing method (ISO 10272-1:2006) involves a high time and labour demand. In this paper, we present a method that reduces the detection time of Campylobacter spp. to or below one third as compared to the ISO method, at a reduced cost per test. We used redox potential change of enrichment cultures (Bolton broth with Bolton selective supplement) for reliably selecting Campylobacter-contaminated raw milk and broiler meat samples. Identification of Campylobacter spp. in the contaminated samples was done by real-time PCR method. Culturing time to conclusive redox monitoring varied between 6 and 24 h for positive samples, depending on the contamination rate, in contrast to 136 h with the standard culturing process. However, now the Campylobacter-negative majority of food samples will not need to be tested by real-time PCR because redox potential monitoring can identify them in the selective enrichment phase. This method could be potentially used as a faster alternative to the current standard ISO 10272-1:2006, for nonregulatory monitoring purposes.

  • Baylis, C. L., MacPhee, S., Martin, K. W., Humphrey, T. J. and Betts, R. P. (2000): Comparison of three enrichment media for the isolation of Campylobacter spp. from foods. J. Appl. Microbiol. 89, 884891.

    • Search Google Scholar
    • Export Citation
  • Chon, J. W., Kim, H., Yim, J. H., Park, J. H., Kim, M. S. and Seo, K. H. (2013): Development of a selective enrichment broth supplemented with bacteriological charcoal and a high concentration of polymyxin B for the detection of Campylobacter jejuni and Campylobacter coli in chicken carcass rinses. Int. J. Food Microbiol. 162, 308310.

    • Search Google Scholar
    • Export Citation
  • Churruca, E., Girbau, C., Martínez, I., Mateo, E., Alonso, R. and Fernández-Astorga, A. (2007): Detection of Campylobacter jejuni and Campylobacter coli in chicken meat samples by real- time nucleic acid sequence-based amplification with molecular beacons. Int. J. Food Microbiol. 117, 8590.

    • Search Google Scholar
    • Export Citation
  • Cocolin, L., Rantsiou, K., Lacumin, L., Cantoni, C. and Comi, G. (2002): Direct identification in food samples of Listeria spp. and Listeria monocytogenes by molecular methods. Appl. Environ. Microbiol. 68, 62736282.

    • Search Google Scholar
    • Export Citation
  • De Boer, P., Rahaoui, H., Leer, R. J., Montijn, R. C. and van der Vossen, J. M. B. M. (2015): Realtime PCR detection of Campylobacter spp.: A comparison to classic culturing and enrichment. Food Microbiol. 51, 96100.

    • Search Google Scholar
    • Export Citation
  • Erdosi, O., Szakmár, K. and Reichart, O. (2014): Rapid detection of Listeria monocytogenes in raw milk and soft cheese by a redox potential measurement based method combined with realtime PCR. Acta Vet. Hung. 62, 304316.

    • Search Google Scholar
    • Export Citation
  • Erdosi, O., Szakmár, K., Reichart, O., Székely-Körmöczy, P. and Laczay, P. (2012): Application of the redox potential measurement based rapid method in the microbial hygienic control. Acta Aliment. Hung. 41, 4555.

    • Search Google Scholar
    • Export Citation
  • European Food Safety Authority (2016): EU summary report on zoonoses, zoonotic agents and food borne outbreaks in 2015. EFSA J. 14, 5057.

    • Search Google Scholar
    • Export Citation
  • Garrido, A., Chapela, M., Román, B., Fajardo, P., Lago, J. and Vieites, J. M. (2013): A new multiplex real-time PCR developed method for Salmonella spp. and Listeria monocytogenes detection in food and environment samples. Food Control. 31, 7685.

    • Search Google Scholar
    • Export Citation
  • Griffiths, P. L. and Park, R. W. A. (1990): Campylobacters associated with human diarrhoeal disease. J. Appl. Bacteriol. 69, 281301.

  • ISO 10272-1:2006: Microbiology of food and animal feeding stuffs. Horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection method.

  • Josefsen, M. H., Bhunia, A. K., Engvall, E. O., Fachmann, M. S. R. and Hoorfar, J. (2015): Monitoring Campylobacter in the poultry production chain –From culture to genes and beyond. J. Microbiol. Meth. 112, 118125.

    • Search Google Scholar
    • Export Citation
  • Kawatsu, K., Taguchi, M., Yonekita, T., Matsumoto, T., Morimatsu, F. and Kumeda, Y. (2010): Simple and rapid detection of Campylobacter spp. in naturally contaminated chicken-meat samples by combination of a two-step enrichment method with an immunochromatographic assay. Int. J. Food Microbiol. 142, 256259.

    • Search Google Scholar
    • Export Citation
  • Kemmeren, J. M., Mangen, M. J., van Duynhoven, Y. T. and Havelaar, A. H. (2005): Priority Setting of Foodborne Pathogens. RIVM Report 330080001. Bilthoven, The Netherlands.

    • Search Google Scholar
    • Export Citation
  • Lynch, O., Cagney, C., McDowell, D. and Duffy, G. (2011): Occurrence of fastidious Campylobacter spp. in fresh meat and poultry using an adapted cultural protocol. Int. J. Food Microbiol. 150, 171177.

    • Search Google Scholar
    • Export Citation
  • Mericon® Pathogen Detection Handbook (2012): For detection of pathogens in food or animal feed samples using real-time PCR. Qiagen®, p. 13.

    • Search Google Scholar
    • Export Citation
  • Moran, L., Kelly, C. and Madden, R. H. (2009): Factors affecting the recovery of Campylobacter spp. from retail packs of raw, fresh chicken using ISO 10272-1:2006. Lett. Appl. Microbiol. 48, 628632.

    • Search Google Scholar
    • Export Citation
  • Navas, J., Ortiz, S., Lopez, P., Jantzen, M. M., Lopez, V. and Martinez-Suarez, J. V. (2006): Evaluation of effects of primary and secondary enrichment for the detection of Listeria monocytogenes by real-time PCR in retail ground chicken meat. Foodborne Pathog. Dis. 3, 347354.

    • Search Google Scholar
    • Export Citation
  • Reichart, O., Szakmár, K., Jozwiak, Á., Felföldi, J. and Baranyai, L. (2007): Redox potential measurement as a rapid method for microbiological testing and its validation for coliform determination. Int. J. Food Microbiol. 114, 143148.

    • Search Google Scholar
    • Export Citation
  • Rijpens, N. P. and Herman, L. M. (2002): Molecular methods for identifications and detection of bacterial food pathogens. J. AOAC Int. 85, 984995.

    • Search Google Scholar
    • Export Citation
  • Robinson, D. A. (1981): Infective dose of Campylobacter jejuni in milk. Brit. Med. J. 282, 1584.

  • Rodríguez-Lázaro, D., Hernández, M. and Pla, M. (2004): Simultaneous quantitative detection of Listeria spp. and Listeria monocytogenes using a duplex real-time PCR-based assay. FEMS Microbiol. Lett. 233, 257267.

    • Search Google Scholar
    • Export Citation
  • Suh, S. H., Dwivedi, H. P. and Jaykus, L. A. (2014): Development and evaluation of aptamer magnetic capture assay in conjunction with real-time PCR for detection of Campylobacter jejuni. LWT –Food Sci. Technol. 56, 256260. Url1: http://www.microtest.hu/?en; microtester

    • Search Google Scholar
    • Export Citation

Author information is available in PDF.
Please, download the file from HERE.

The manuscript preparation instructions is available in PDF.
Please, download the file from HERE.

Senior editors

Editor-in-Chief: Mária BENKŐ

Managing Editor: András SZÉKELY

Editorial Board

  • Béla DÉNES (National Food Chain Safety Office, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (National Agricultural Innovation Centre, Herceghalom, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 467 4081 (ed.-in-chief) or (36 1) 213 9793 (editor) Fax: (36 1) 467 4076 (ed.-in-chief) or (36 1) 213 9793

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture, Biology and Environmental Sciences
  • Elsevier Science Navigator
  • Focus On: Veterinary Science and Medicine
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

2020  
Total Cites 987
WoS
Journal
Impact Factor
0,955
Rank by Veterinary Sciences 101/146 (Q3)
Impact Factor  
Impact Factor 0,920
without
Journal Self Cites
5 Year 1,164
Impact Factor
Journal  0,57
Citation Indicator  
Rank by Journal  Veterinary Sciences 93/166 (Q3)
Citation Indicator   
Citable 49
Items
Total 49
Articles
Total 0
Reviews
Scimago 33
H-index
Scimago 0,395
Journal Rank
Scimago Veterinary (miscellaneous) Q2
Quartile Score  
Scopus 355/217=1,6
Scite Score  
Scopus General Veterinary 73/183 (Q2)
Scite Score Rank  
Scopus 0,565
SNIP  
Days from  145
submission  
to acceptance  
Days from  150
acceptance  
to publication  
Acceptance 19%
Rate

 

2019  
Total Cites
WoS
798
Impact Factor 0,991
Impact Factor
without
Journal Self Cites
0,897
5 Year
Impact Factor
1,092
Immediacy
Index
0,119
Citable
Items
59
Total
Articles
59
Total
Reviews
0
Cited
Half-Life
9,1
Citing
Half-Life
9,2
Eigenfactor
Score
0,00080
Article Influence
Score
0,253
% Articles
in
Citable Items
100,00
Normalized
Eigenfactor
0,09791
Average
IF
Percentile
42,606
Scimago
H-index
32
Scimago
Journal Rank
0,372
Scopus
Scite Score
335/213=1,6
Scopus
Scite Score Rank
General Veterinary 62/178 (Q2)
Scopus
SNIP
0,634
Acceptance
Rate
18%

 

Acta Veterinaria Hungarica
Publication Model Hybrid
Submission Fee none
Article Processing Charge 1100 EUR/article
Printed Color Illustrations 40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2022 Online subsscription: 710 EUR / 892 USD
Print + online subscription: 824 EUR / 1028 USD
Subscription Information Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation
1951
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2021 3 0 0
Sep 2021 6 0 0
Oct 2021 6 0 0
Nov 2021 6 1 1
Dec 2021 6 0 0
Jan 2022 25 1 0
Feb 2022 0 0 0