The predominance of dietary viruses in bat guano samples had been described recently, suggesting a new opportunity to survey the prevalence and to detect new viruses of arthropods or even plant-infecting viruses circulating locally in the ecosystem. Here we describe the diversity of viruses belonging to the order Picornavirales in Hungarian insectivorous bat guano samples. The metagenomic analysis conducted on our samples has revealed the significant predominance of aphid lethal paralysis virus (ALPV) and Big Sioux River virus (BSRV) in Hungary for the first time. Phylogenetic analysis was used to clarify the relationship to previously identified ALPV strains infecting honey bees, showing that our strain possesses a close genetic relationship with the strains that have already been described as pathogenic to honey bees. Furthermore, studies have previously confirmed the ability of these viruses to replicate in adult honey bees; however, no signs related to these viruses have been revealed yet. With the identification of two recently described possibly honey bee infecting viruses for the first time in Hungary, our results might have importance for the health conditions of Hungarian honey bee colonies in the future.
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Bakonyi, T., Farkas, R., Szendröi, A., Dobos-Kovács, M. and Rusvai, M. (2002): Detection of acute bee paralysis virus by RT-PCR in honey bee and Varroa destructor field samples: rapid screening of representative Hungarian apiaries. Apidologie 33, 63–74.
-
Bányai, K., Kemenesi, G., Budinski, I., Földes, F., Zana, B., Marton, S., Varga-Kugler, R., Oldal, M., Kurucz, K. and Jakab, F. (2016): Candidate new rotavirus species in Schreiber’s bats, Serbia. Infect. Genet. Evol. 48, 19–26.
-
Calisher, C. H., Childs, J. E., Field, H. E., Holmes, K. V. and Schountz, T. (2006): Bats: important reservoir hosts of emerging viruses. Clin. Microbiol. Rev. 19, 531–545.
-
Conceição-Neto, N., Zeller, M., Lefrère, H., De Bruyn, P., Beller, L., Deboutte, W., Yinda, C. K., Lavigne, R., Maes, P., Van Ranst, M., Heylen, E. and Matthijnssens, J. (2015): Modular approach to customise sample preparation procedures for viral metagenomics: a reproducible protocol for virome analysis. Sci. Rep. 5, 16532.
-
Cornman, R. S., Tarpy, D. R., Chen, Y., Jeffreys, L., Lopez, D., Pettis, J. S., vanEngelsdorp, D. and Evans, J. D. (2012): Pathogen webs in collapsing honey bee colonies. PLoS ONE 7, e43562.
-
Dacheux, L., Cervantes-Gonzalez, M., Guigon, G., Thiberge, J. M., Vandenbogaert, M., Maufrais, C., Caro, V. and Bourhy, H. (2014): A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses. PLoS ONE 9, e87194.
-
Donaldson, E. F., Haskew, A. N., Gates, J. E., Huynh, J., Moore, C. J. and Frieman, M. B. (2010): Metagenomic analysis of the viromes of three North American bat species: viral diversity among different bat species that share a common habitat. J. Virol. 84, 13004–13018.
-
Edgar, R. C. (2004): MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucl. Acids Res. 32, 1792–1797.
-
Forgách, P., Bakonyi, T., Tapaszti, Z., Nowotny, N. and Rusvai, M. (2008): Prevalence of pathogenic bee viruses in Hungarian apiaries: situation before joining the European Union. J. Invertebr. Pathol. 98, 235–238.
-
Ge, X., Li, Y., Yang, X., Zhang, H., Zhou, P., Zhang, Y. and Shi, Z. (2012): Metagenomic analysis of viruses from bat fecal samples reveals many novel viruses in insectivorous bats in China. J. Virol. 86, 4620–4630.
-
Granberg, F., Vicente-Rubiano, M., Rubio-Guerri, C., Karlsson, O. E., Kukielka, D., Belák, S. and Sánchez-Vizcaíno, J. M. (2013): Metagenomic detection of viral pathogens in Spanish honeybees: co-infection by Aphid Lethal Paralysis, Israel Acute Paralysis and Lake Sinai Viruses. PLoS ONE 8, e57459.
-
He, B., Li, Z., Yang, F., Zheng, J., Feng, Y., Guo, H., Li, Y., Wang, Y., Su, N., Zhang, F., Fan, Q. and Tu, C. (2013): Virome profiling of bats from Myanmar by metagenomic analysis of tissue samples reveals more novel mammalian viruses. PLoS ONE 8, e61950.
-
Kemenesi, G., Dallos, B., Görföl, T., Boldogh, S., Estók, P., Kurucz, K., Kutas, A., Földes, F., Oldal, M., Németh, V., Martella, V., Bányi, K. and Jakab, F. (2014): Molecular survey of RNA viruses in Hungarian bats: discovering novel astroviruses, coronaviruses, and caliciviruses. Vector Borne Zoonotic Dis. 14, 846–855.
-
Kemenesi, G., Dallos, B., Görföl, T., Estók, P., Boldogh, S., Kurucz, K., Oldal, M., Marton, S., Bányai, K. and Jakab, F. (2015 a): Genetic diversity and recombination within bufaviruses: Detection of a novel strain in Hungarian bats. Infect. Genet. Evol. 33, 288–292.
-
Kemenesi, G., Földes, F., Zana, B., Kurucz, K., Estók, P., Boldogh, S., Görföl, T., Bányai, K., Oldal, M. and Jakab, F. (2016): Genetic characterization of Providence virus isolated from bat guano in Hungary. Genome Announc. 4, e00403-16.
-
Kemenesi, G., Zhang, D., Marton, S., Dallos, B., Görföl, T., Estók, P., Boldogh, S., Kurucz, K., Oldal, M., Kutas, A., Bányai, K. and Jakab, F. (2015 b): Genetic characterization of a novel picornavirus detected in Miniopterus schreibersii bats. J. Gen. Virol. 96, 815–821.
-
Lee, K., Steinhauer, N., Travis, D. A., Meixner, M. D. and Deen, J. (2015): Honey bee surveillance: a tool for understanding and improving honey bee health. Curr. Opin. Insect Sci. 10, 37–44.
-
Li, L., Victoria, J. G., Wang, C., Jones, M., Fellers, G. M., Kunz, T. H. and Delwart, E. (2010): Bat guano virome: predominance of dietary viruses from insects and plants plus novel mammalian viruses. J. Virol. 84, 6955–6965.
-
Liu, S., Vijayendran, D., Carrillo-Tripp, J., Miller, W. A. and Bonning, B. C. (2014): Analysis of new aphid lethal paralysis virus (ALPV) isolates suggests evolution of two ALPV species. J. Gen. Virol. 95, 2809–2819.
-
McMenamin, A. J. and Genersch, E. (2015): Honey bee colony losses and associated viruses. Curr. Opin. Insect Sci. 8, 121–129.
-
Moon, J. S., Domier, L. L., McCoppin, N. K., D’Arcy, C. J. and Jin, H. (1998): Nucleotide sequence analysis shows that Rhopalosiphum padi virus is a member of a novel group of insect- infecting RNA viruses. Virology 243, 54–65.
-
Mordecai, G. J., Brettell, L. E., Pachori, P., Villalobos, E. M., Martin, S. J., Jones, I. M. and Schroeder, D. C. (2016): Moku virus; a new Iflavirus found in wasps, honey bees and Varroa. Sci. Rep. 6, 34983.
-
OMME–Országos Magyar Méhészeti Egyesület [National Hungarian Beekeeping Association] (2017): Magyar Méhészeti Nemzeti Program, Környezetterhelési Monitoringvizsgálat 2016–2017 [Hungarian National Beekeeping Programme, Environmental Impact Monitoring Survey 2016–2017]. Accessed 22 January 2018. Retrieved from: http://www.omme.hu/?p=9982
-
Ravoet, J., De Smet, L., Wenseleers, T. and de Graaf, D. C. (2015): Vertical transmission of honey bee viruses in Belgian queen breeding program. BMC Vet. Res. 11, 61.
-
Ravoet, J., Maharramov, J., Meeus, I., De Smet, L., Wenseleers, T., Smagghe, G. and de Graaf, D. C. (2013): Comprehensive bee pathogen screening in Belgium reveals Crithidia mellificae as a new contributory factor to winter mortality. PLoS ONE 8, e72443.
-
Runckel, C., Flenniken, M. L., Engel, J. C., Ruby, J. G., Ganem, D., Andino, R. and DeRisi, J. L. (2011): Temporal analysis of the honey bee microbiome reveals four novel viruses and seasonal prevalence of known viruses, Nosema, and Crithidia. PLoS ONE 6, e20656.
-
Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. (2013): MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol. 30, 2725–2729.
-
Tapaszti, Z., Forgách, P., Kovágó, C., Békési, L., Bakonyi, T. and Rusvai, M. (2009): First detection and dominance of Nosema ceranae in Hungarian honeybee colonies. Acta Vet. Hung. 57, 383–388.
-
Vanengelsdorp, D., Evans, J. D., Saegerman, C., Mullin, C., Haubruge, E., Nguyen, B. K., Frazier, M., Frazier, J., Cox-Foster, D., Chen, Y., Underwood, R., Tarpy, D. R. and Pettis, J. S. (2009): Colony collapse disorder: a descriptive study. PLoS ONE 4, e6481.
-
Wang, J., Moore, M. E., Murray, Z. L., McInnes, K., White, D. J., Tompkins, D. M. and Hall, R. J. (2015): Discovery of novel virus sequences in an isolated and threatened bat species, the New Zealand lesser short-tailed bat (Mystacina tuberculata). J. Gen. Virol. 96, 2442–2452.
-
Wu, Z., Yang, L., Ren, X., He, G., Zhang, J., Yang, J., Qian, Z., Dong, J., Sun, L., Zhu, Y., Du, J., Yang, F., Zhang, S. and Jin, Q. (2016): Deciphering the bat virome catalog to better understand the ecological diversity of bat viruses and the bat origin of emerging infectious diseases. ISME J. 10, 609–620.
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