First molecular detection of ball python nidovirus in Italy – Short communication

Elisa Rampacci; Marco Masi; Francesco Carlo Origgi; Valentina Stefanetti; Marco Bottinelli; Paolo Selleri; Mauro Coletti; Fabrizio Passamonti

doi:10.1556/004.2019.014

Jump to Content

Acta Veterinaria Hungarica

Volume/Issue:: Volume 67: Issue 1

First molecular detection of ball python nidovirus in Italy – Short communication

Authors:

Elisa Rampacci

Elisa Rampacci Department of Veterinary Medicine, University of Perugia, San Costanzo 4, 06126, Perugia, Italy

Search for other papers by Elisa Rampacci in
Current site
Google Scholar
PubMed

Marco Masi

Marco Masi Centro Veterinario Specialistico, Rome, Italy

Search for other papers by Marco Masi in
Current site
Google Scholar
PubMed

Francesco Carlo Origgi

Francesco Carlo Origgi Department of Infectious Diseases and Pathobiology, Centre for Fish and Wildlife Health, University of Bern, Bern, Switzerland

Search for other papers by Francesco Carlo Origgi in
Current site
Google Scholar
PubMed

Valentina Stefanetti

Valentina Stefanetti Department of Veterinary Medicine, University of Perugia, San Costanzo 4, 06126, Perugia, Italy

Search for other papers by Valentina Stefanetti in
Current site
Google Scholar
PubMed

Marco Bottinelli

Marco Bottinelli Department of Veterinary Medicine, University of Perugia, San Costanzo 4, 06126, Perugia, Italy

Search for other papers by Marco Bottinelli in
Current site
Google Scholar
PubMed

Paolo Selleri

Paolo Selleri Centro Veterinario Specialistico, Rome, Italy

Search for other papers by Paolo Selleri in
Current site
Google Scholar
PubMed

Mauro Coletti

Mauro Coletti Department of Veterinary Medicine, University of Perugia, San Costanzo 4, 06126, Perugia, Italy

Search for other papers by Mauro Coletti in
Current site
Google Scholar
PubMed

, and

Fabrizio Passamonti

Fabrizio Passamonti Department of Veterinary Medicine, University of Perugia, San Costanzo 4, 06126, Perugia, Italy

Search for other papers by Fabrizio Passamonti in
Current site
Google Scholar
PubMed

View More View Less

Pages:: 127–134

Online Publication Date:: Mar 2019

Publication Date:: 01 Mar 2019

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/004.2019.014

Keywords:: Ball python nidovirus; ferlavirus; respiratory disease; RT-PCR; phylogeny reconstruction

Restricted access

Purchase article

USD $25.00

1 year subscription (Individual Only)

USD $836.00

USD $25.00

Click here to view the full Terms and Conditions

USD $836.00

Click here to view the full Terms and Conditions

A retrospective study was conducted to investigate the presence of ferlavirus, ball python nidovirus and bacteria in 32 tracheobronchial lavages from ball pythons raised in captivity and affected by respiratory disease. A touchdown reverse transcription polymerase reaction (RT-PCR) was performed to detect ball python nidovirus RNA targeting a 260-bp portion of the ORF1a gene, while a nested RT-PCR was applied to identify RNA targeting the 518-bp ferlavirus partial L gene. RT-PCR positive products were submitted for Sanger’s sequencing and phylogeny reconstruction. Bacteriological examinations were performed to diagnose a possible bacterial involvement. BLAST analysis revealed that the nucleotide sequences of the six (18.8%) RT-PCR positive amplicons were 90–97% identical to the partial sequence of the ORF1a gene of the recently described ball python nidovirus. All tested snakes were negative for ferlavirus. Thirteen out of 32 samples (40.6%) were bacteriologically positive. Respiratory tract diseases can be a substantial problem for snake breeders, considering the rapid transmission of respiratory pathogens. The results and published studies show that ball python nidovirus is circulating in python collections and could be linked to suboptimal management practices. Surveillance programs are desirable as part of the routine snake health assessment. Tracheobronchial lavage is a fast, practical, cost-effective procedure for sample collection.

Save
Cite
Email this content

Share Link

Copy this link, or click below to email it to a friend
Email this content
or copy the link directly:

https://akjournals.com/abstract/journals/004/67/1/article-p127.xml
The link was not copied. Your current browser may not support copying via this button.

Link copied successfully

Collapse
Expand

Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

Submit Your Manuscript

Author informations are available in PDF.
Please, download the file from HERE

The manuscript preparation instructions are available in PDF.
Please, download the file from HERE

Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 287 7073 (ed.-in-chief) or (36 1) 467 4081 (editor)

E-mail: acta.veterinaria@univet.hu (ed.-in-chief)

Indexing and Abstracting Services:

Biological Abstracts
BIOSIS Previews
CAB Abstracts
Chemical Abstracts
Current Contents: Agriculture, Biology and Environmental Sciences
Elsevier Science Navigator
Focus On: Veterinary Science and Medicine
Global Health
Index Medicus
Index Veterinarius
Medline
Science Citation Index
Science Citation Index Expanded (SciSearch)
SCOPUS
The ISI Alerting Services
Zoological Abstracts

2023
Web of Science
Journal Impact Factor	0.7
Rank by Impact Factor	Q3 (Veterinary Sciences)
Journal Citation Indicator	0.4
Scopus
CiteScore	1.8
CiteScore rank	Q2 (General Veterinary)
SNIP	0.39
Scimago
SJR index	0.258
SJR Q rank	Q3

Acta Veterinaria Hungarica
Publication Model	Hybrid
Submission Fee	none
Article Processing Charge	1100 EUR/article
Printed Color Illustrations	40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency	World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100%
Further Discounts	Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2025	Online subsscription: 832 EUR / 916 USD Print + online subscription: 960 EUR / 1054 USD
Subscription Information	Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title	Individual articles are sold on the displayed price.

Acta Veterinaria Hungarica
Language	English
Size	A4
Year of Foundation	1951
Volumes per Year	1
Issues per Year	4
Founder	Magyar Tudományos Akadémia
Founder's Address	H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher	Akadémiai Kiadó
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-6290 (Print)
ISSN	1588-2705 (Online)