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  • 1 University of Novi Sad, Trg Dositeja Obradovića 8, 21 000 Novi Sad, Serbia
  • 2 University of Belgrade, Belgrade, Serbia
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Consumption of undercooked or raw pork is considered a significant risk factor for human infection with Toxoplasma gondii. In this study, we investigated the genetic structure of 18 T. gondii strains obtained from slaughter pigs from Northern Serbia (mainly Vojvodina). The examined samples originated from eight pigs from large commercial farms, six backyard pigs and four free-range Mangalica pigs, all found to be positive for either viable T. gondii or T. gondii DNA. Genotyping was attempted from both pig tissues and mouse brains from the bio-assays using a multiplex multilocus nested polymerase chain reaction–restriction fragment length polymorphism (Mn-PCR-RFLP) method with seven markers (GRA6, alt. SAG2, PK-1, BTUB, C22-8, CS3 and Apico). Identification was achieved for nine T. gondii isolates. Seven isolates were classified as type II and two as type III. These results are consistent with previous studies on animal isolates from Serbia as well as with previous reports that type III is more frequently found in samples from Southern Europe than in those from other parts of the continent.

  • Bacci, C., Vismarra, A., Mangia, C., Bonardi, S., Bruini, I., Genchi, M., Kramer, L. and Brindani, F. (2015): Detection of Toxoplasma gondii in free-range, organic pigs in Italy using serological and molecular methods. Int. J. Food Microbiol. 202, 5456.

    • Search Google Scholar
    • Export Citation
  • Dardé, M.-L. (2008): Toxoplasma gondii, ‘new’ genotypes and virulence. Parasite. 15, 366371.

  • De Sousa, S., Ajzenberg, D., Canada, N., Freire, L., Da Costa, J. M. C., Darde, M. L., Thulliez, P. and Dubey, J. P. (2006): Biologic and molecular characterization of Toxoplasma gondii isolates from pigs from Portugal. Vet. Parasitol. 135, 133136.

    • Search Google Scholar
    • Export Citation
  • Djokic, V., Blaga, R., Aubert, D., Durand, B., Perret, C., Geers, R., Ducry, T., Vallee, I., Djurković-Djaković, O., Mzabi, A., Villena, I. and Boireau, P. (2016): Toxoplasma gondii infection in pork produced in France. Parasitol. 143, 557567.

    • Search Google Scholar
    • Export Citation
  • Djurković-Djaković, O., Klun, I., Khan, A., Nikolić, A., Knežević-Ušaj, S., Bobić, B. and Sibley, L. D. (2006): A human origin type II strain of Toxoplasma gondii causing severe encephalitis in mice. Microb. Infect. 8, 22062212.

    • Search Google Scholar
    • Export Citation
  • Dubey, J. P., Lunney, J. K., Shen, S. K., Kwok, O. C., Ashford, D. and Thulliez, P. (1996): Infectivity of low numbers of Toxoplasma gondii oocysts to pigs. J. Parasitol. 82, 438443.

    • Search Google Scholar
    • Export Citation
  • Dubey, J. P., Vianna, M. C., Sousa, S., Canada, N., Meireles, S., Correia da Costa, J. M., Marcet, P. L., Lehmann, T., Dardé, M. L. and Thulliez, P. (2006): Characterization of Toxoplasma gondii isolates in free-range chickens from Portugal. J. Parasitol. 92, 184186.

    • Search Google Scholar
    • Export Citation
  • EFSA (2011): Scientific Opinion on the public health hazards to be covered by inspection of meat (swine). EFSA J. 9, 1198.

  • Howe, D. K. and Sibley, L. D. (1995): Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease. J. Infect. Dis. 172, 15611566.

    • Search Google Scholar
    • Export Citation
  • Khan, A., Dubey, J. P., Su, C., Ajioka, J. W., Rosenthal, B. M. and Sibley, L. D. (2011): Genetic analyses of atypical Toxoplasma gondii strains reveal a fourth clonal lineage in North America. Int. J. Parasitol. 41, 645655.

    • Search Google Scholar
    • Export Citation
  • Klun, I., Uzelac, A., Villena, I., Mercier, A., Bobić, B., Nikolić, A., Rajnpreht, I., Opsteegh, M., Aubert, D., Blaga, R., Van der Giessen, J. and Djurković-Djaković, O. (2017): The first isolation and molecular characterization of Toxoplasma gondii from horses in Serbia. Parasit. Vectors 10, 167.

    • Search Google Scholar
    • Export Citation
  • Kuruca, L., Klun, I., Uzelac, A., Nikolić, A., Bobić, B., Simin, S., Djurković-Djaković, O. and Lalošević, V. (2016): Detection of viable Toxoplasma gondii in free-range pigs from the Special Nature Reserve of Zasavica. Contemp. Agric. 65, 16.

    • Search Google Scholar
    • Export Citation
  • Kuruca, L., Klun, I., Uzelac, A., Nikolić, A., Bobić, B., Simin, S., Lalošević, D., Lalošević, V. and Djurković-Djaković, O. (2017): Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia. Parasitol. Res. 116, 31173123.

    • Search Google Scholar
    • Export Citation
  • Marković, M., Ivović, V., Štajner, T., Djokić, V., Klun, I., Bobić, B., Nikolić, A. and Djurković-Djaković, O. (2014): Evidence for genetic diversity of Toxoplasma gondii in selected intermediate hosts in Serbia. Comp. Immunol. Microbiol. Infect. Dis. 37, 173179.

    • Search Google Scholar
    • Export Citation
  • Mercier, A., Devillard, S., Ngoubangoye, B., Bonnabau, H., Bañuls, A. L., Durand, P., Salle, B., Ajzenberg, D. and Dardé, M. L. (2010): Additional haplogroups of Toxoplasma gondii out of Africa: Population structure and mouse-virulence of strains from Gabon. PLoS Negl. Trop. Dis. 4, e876.

    • Search Google Scholar
    • Export Citation
  • Pena, H. F. J., Gennari, S. M., Dubey, J. P. and Su, C. (2008): Population structure and mouse-virulence of Toxoplasma gondii in Brazil. Int. J. Parasitol. 38, 561569.

    • Search Google Scholar
    • Export Citation
  • Shwab, E. K., Zhu, Xi.-Q., Majumdar, D., Pena, H. F. J., Gennari, S. M., Dubey, J. P. and Su, C. (2014): Geographical patterns of Toxoplasma gondii genetic diversity revealed by multilocus PCR-RFLP genotyping. Parasitol. 141, 453461.

    • Search Google Scholar
    • Export Citation
  • Sibley, L. D., Khan, A., Ajioka, J. W. and Rosenthal, B. M. (2009): Genetic diversity of Toxoplasma gondii in animals and humans. Philos. Trans. R. Soc. Lond. B Biol. Sci. 364, 27492761.

    • Search Google Scholar
    • Export Citation
  • Slany, M., Reslova, N., Babak, V. and Lorencova, A. (2016): Molecular characterization of Toxoplasma gondii in pork meat from different production systems in the Czech Republic. Int. J. Food Microbiol. 238, 252255.

    • Search Google Scholar
    • Export Citation
  • Štajner, T., Vasiljević, Z., Vujić, D., Marković, M., Ristić, G., Mićić, D., Pašić, S., Ivović, V., Ajzenberg, D. and Djurković-Djaković, O. (2013): Atypical strain of Toxoplasma gondii causing fatal reactivation after hematopoietic stem cell transplantation in a patient with an underlying immunological deficiency. J. Clin. Microbiol. 51, 26862690.

    • Search Google Scholar
    • Export Citation
  • Su, C., Shwab, E. K., Zhou, P., Zhu, X. Q. and Dubey, J. P. (2010): Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii. Parasitol. 137, 111.

    • Search Google Scholar
    • Export Citation
  • Su, C., Zhang, X. and Dubey, J. P. (2006): Genotyping of Toxoplasma gondii by multilocus PCR-RFLP markers: A high resolution and simple method for identification of parasites. Int. J. Parasitol. 36, 841848.

    • Search Google Scholar
    • Export Citation
  • Vergara, A., Marangi, M., Caradonna, T., Pennisi, L., Paludi, D., Papini, R., Ianieri, A., Giangaspero, A. and Normanno, G. (2018): Toxoplasma gondii lineages circulating in slaughtered industrial pigs and potential risk for consumers. J. Food Prot. 81, 13731378.

    • Search Google Scholar
    • Export Citation
  • Vujanić, M. (2012): Molecular detection and genotyping of Toxooplasma gondii strains isolated in Serbia. PhD Thesis. Faculty of Biology, University of Belgrade, Belgrade, Serbia.

    • Search Google Scholar
    • Export Citation
  • Wang, L., Cheng, H., Huang, K., Xu, Y., Li Y., Du, J., Yu, L., Luo, Q., Wei, W., Jiang, L. and Shen, J. (2013): Toxoplasma gondii prevalence in food animals and rodents in different regions of China: isolation, genotyping and mouse pathogenicity. Parasit. Vectors 6, 273.

    • Search Google Scholar
    • Export Citation

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