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  • 1 University of La Laguna, Campus de Ofra S/N, Santa Cruz de Tenerife 38071, Spain
  • | 2 University Hospital of the Canary Islands, San Cristobal de La Laguna, Tenerife, Canary Islands, Spain
  • | 3 Canary Islands Health Service, Santa Cruz de Tenerife, Canary Islands, Spain
  • | 4 Institute of Care Research, Nurses Association of Santa Cruz de Tenerife, Spain
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The aim of this study was to determine the prevalence of methicillin-resistant Staphylococcus (MRS) in healthy goats on the Island of Tenerife, Spain, as well as to identify the phenotypic and genotypic characteristics of the strains found. A cross-sectional prevalence study was conducted. A total of 158 goats from 15 different farms were sampled between September 2017 and January 2018. The percentage of positive samples of methicillin-resistant Staphylococcus aureus (MRSA) was 15.8% (25/158) and that of methicillin-resistant coagulase-negative staphylococci (MRCoNS) was 6.9% (11/158). All MRSA isolates from goats belonged to one clonal group showing Multi-Locus Sequence type 398. All strains studied (n = 36) were resistant to non-carbapenem beta-lactam antibiotics and susceptible to teicoplanin, linezolid, vancomycin, rifampicin, quinupristin-dalfospristin and mupirocine. In MRSA isolates, the highest percentage of resistance obtained, besides beta-lactam non-carbapenem antibiotics, was to trimethoprim-sulphamethoxazole and, in the case of MRCoNS isolates, to phosphomycin and erythromycin. A total of 12 resistance patterns were obtained, presenting differences between patterns obtained for MRSA and MRCoNS, with 7 different patterns for MRSA and 5 for MRCoNS. We therefore consider it essential to expand the epidemiological study of these strains of animal origin, as well as to increase surveillance and control measures at all stages of the food chain.

  • Alves, P. D., McCulloch, J. A., Even, S., Le Maréchal, C., Thierry, A., Grosset, N., Azevedo, V., Rosa, C. A., Vautor, E. and Le Loir, Y. (2009): Molecular characterisation of Staphylococcus aureus strains isolated from small and large ruminants reveals a host rather than tissue specificity. Vet. Microbiol. 137, 190195. doi: 10.1016/j.vetmic.2008.12.014

    • Search Google Scholar
    • Export Citation
  • Anker, J. C. H., Koch, A., Ethelberg, S., Mølbak, K., Larsen, J. and Jepsen, M. R. (2018): Distance to pig farms as risk factor for community-onset livestock-associated MRSA CC398 infection in persons without known contact to pig farms. A nationwide study. Zoonoses Public Health 65, 352360. doi: 10.1111/zph.12441

    • Search Google Scholar
    • Export Citation
  • Aquino, V., Maluta, R. P. and de Ávila, F. A. (2012): Prevalence of methicillin-resistant staphylococci on a farm: staff can harbour MRS when animals do not. Zoonoses Public Health 59, 13. doi: 10.1111/j.1863-2378

    • Search Google Scholar
    • Export Citation
  • Bhargava, K. and Zhang, Y. (2014): Characterization of methicillin-resistant coagulase-negative staphylococci (MRCoNS) in retail meat. Food Microbiol. 42, 5660. doi: 10.1016/j.fm.2014.02.019

    • Search Google Scholar
    • Export Citation
  • Chu, C., Yu, C., Lee, Y. and Su, Y. (2012): Genetically divergent methicillin-resistant Staphylococcus aureus and sec-dependent mastitis of dairy goats in Taiwan. BMC Vet. Res. 29, 39. doi: 10.1186/1746-6148-8-39

    • Search Google Scholar
    • Export Citation
  • Chuang, Y. Y. and Huang, Y. C. (2015): Livestock-associated methicillin-resistant Staphylococcus aureus in Asia: an emerging issue? Int. J. Antimicrob. Agents 45, 334340. doi: 10.1016/j.ijantimicag.2014.12.007

    • Search Google Scholar
    • Export Citation
  • Clinical Laboratory Standards Institute (2011): Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement Document M100-S21. CLSI, Wayne, PA. Available online: http://www.clsi.org/

    • Search Google Scholar
    • Export Citation
  • Cortimiglia, C., Bianchini, V., Franco, A., Caprioli, A., Battisti, A., Colombo, L., Stradiotto, K., Vezzoli, F. and Luini, M. (2015): Short communication: Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus in bulk tank milk from dairy goat farms in Northern Italy. J. Dairy Sci. 98, 2307-2311. doi: 10.3168/jds.2014-8923

    • Search Google Scholar
    • Export Citation
  • Enright, M. C., Day, N. P. J., Davies, C. E., Peacock, S. J. and Spratt, B. G. (2000): Multilocus Sequence Typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38, 10081015.

    • Search Google Scholar
    • Export Citation
  • European Commission (2016): Report 2016-8978. DG (SANTE) 2016-8887. Available in: http://ec.europa.eu/food/audits-analysis/overview_reports

    • Search Google Scholar
    • Export Citation
  • Feld, L., Bay, H., Angen, Ø., Larsen, A. R. and Madsen, A. M. (2018): Survival of LA-MRSA in dust from swine farms. Ann. Work. Expo. Health 62, 147156. doi: 10.1093/annweh/wxx108

    • Search Google Scholar
    • Export Citation
  • Fernandes Dos Santos, F., Mendonça, L. C., Reis, D. R. L., Guimarães, A. S., Lange, C. C., Ribeiro, J. B., Machado, M. A. and Brito, M. A. V. P. (2016): Presence of mecA-positive multidrug-resistant Staphylococcus epidermidis in bovine milk samples in Brazil. J. Dairy Sci. 99, 13741382. doi: 10.3168/jds.2015-9931

    • Search Google Scholar
    • Export Citation
  • Goerge, T., Lorenz, M. B., van Alen, S., Hübne, N. O., Becker, K. and Köck, R. (2017): MRSA colonization and infection among persons with occupational livestock exposure in Europe: Prevalence, preventive options and evidence. Vet. Microbiol. 200, 612. doi: 10.1016/j.vetmic.2015.10.027

    • Search Google Scholar
    • Export Citation
  • Habrun, B., Račić, I., Beck, R., Budimir, A., Benić, M., Kompes, G., Špičić, S. and Cvetnić, Z. (2011): The presence of methicillin-resistant Staphylococcus aureus on large pig breeding farms in Croatia. Acta Vet. Hung. 59, 419425. doi: 10.1556/AVet.2011.028

    • Search Google Scholar
    • Export Citation
  • Hernández-Porto, M., Castro, B., Ramos, M. J., Arias, A., Aguirre-Jaime, A. and Lecuona, M. (2014): Risk factors for development of methicillin-resistant Staphylococcus aureus-positive clinical culture in nasal carriers after decolonization treatment. Am. J. Infec. Control 42, e7579. doi: 10.1016/j.ajic.2014.03.011

    • Search Google Scholar
    • Export Citation
  • Huber, H., Ziegler, D., Pflüger, V., Vogel, G., Zweifel, C. and Stephan, R. (2011): Prevalence and characteristics of methicillin-resistant coagulase-negative staphylococci from livestock, chicken carcasses, bulk tank milk, minced meat, and contact persons. BMC Vet. Res. 7, 6. doi: 10.1186/1746-6148-7-6

    • Search Google Scholar
    • Export Citation
  • Landeta, G., Curiel, J. A., Carrascosa, A. V., Muñoz, R. and de las Rivas, B. (2013): Characterization of coagulase-negative staphylococci isolated from Spanish dry cured meat products. Meat Sci. 93, 387396. doi: 10.1016/j.meatsci.2012.09.019

    • Search Google Scholar
    • Export Citation
  • Loncaric, I., Brunthaler, R. and Spergser, J. (2013): Suspected goat-to-human transmission of methicillin-resistant Staphylococcus aureus sequence type 398. J. Clin. Microbiol. 51, 16251626. doi: 10.1128/JCM.03052-12

    • Search Google Scholar
    • Export Citation
  • Mahato, S., Mistry, H. U., Chakraborty, S., Sharma, P., Saravanan, R. and Bhandari, V. (2017): Identification of variable traits among the methicillin resistant and sensitive coagulase negative staphylococci in milk samples from mastitic cows in India. Front. Microbiol. 8, 1446. doi: 10.3389/fmicb.2017.01446.

    • Search Google Scholar
    • Export Citation
  • Morcillo, A., Castro, B., Rodríguez-Álvarez, C., González, J. C., Sierra, A., Montesinos, M. I., Abreu, R. and Arias, Á. (2012): Prevalence and characteristics of methicillin-resistant Staphylococcus aureus in pigs and pig workers in Tenerife, Spain. Foodborne Pathog. Dis. 9, 207210. doi: 10.1089/fpd.2011

    • Search Google Scholar
    • Export Citation
  • Nemeghaire, S., Vanderhaeghen, W., Argudín, M. A., Haesebrouck, F. and Butaye, P. (2014): Characterization of methicillin-resistant Staphylococcus sciuri isolates from industrially raised pigs, cattle and broiler chickens. J. Antimicrob. Chemother. 69, 29282934. doi: 10.1093/jac/dku268

    • Search Google Scholar
    • Export Citation
  • Obaidat, M. M., Bani Salman, A. E. and Roess, A. A. (2018): High prevalence and antimicrobial resistance of mecA Staphylococcus aureus in dairy cattle, sheep, and goat bulk tank milk in Jordan. Trop. Anim. Health. Prod. 50, 405412. doi: 10.1007/s11250-017-1449-7

    • Search Google Scholar
    • Export Citation
  • Ortwine, J. K. and Bhavan, K. (2018): Morbidity, mortality, and management of methicillin-resistant S. aureus bacteremia in the USA : update on antibacterial choices and understanding. Hospital Pract. (1995) 46, 6472. doi: 10.1080/21548331.2018.1435128

    • Search Google Scholar
    • Export Citation
  • Parisi, A., Caruso, M., Normanno, G., Latorre, L., Sottili, R., Miccolupo, A., Fraccalvieri, R. and Santagada, G. (2016): Prevalence, antimicrobial susceptibility and molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) in bulk tank milk from southern Italy. Food Microbiol. 58, 3642. doi: 10.1016/j.fm.2016.03.004

    • Search Google Scholar
    • Export Citation
  • Pletinckx, L. J., Verhegghe, M., Crombé, F., Dewulf, J., De Bleecker, Y., Rasschaert, G., Butaye, P., Goddeeris, B. M. and De Man, I. (2013): Evidence of possible methicillin-resistant Staphylococcus aureus ST398 spread between pigs and other animals and people residing on the same farm. Prev. Vet. Med. 109, 293303. doi: 10.1016/j.prevetmed.2012.10.019

    • Search Google Scholar
    • Export Citation
  • Pomba, C., Rantala, M., Greko, C., Baptiste, K. E., Catry, B., van Duijkeren, E., Mateus, A., Moreno, M. A., Pyörälä, S., Ružauskas, M., Sanders, P., Teale, C., Threlfall, E. J., Kunsagi, Z., Torren-Edo, J., Jukes, H. and Törneke, K. (2017): Public health risk of antimicrobial resistance transfer from companion animals. J. Antimicrob. Chemother. 72, 957968. doi: 10.1093/jac/dkw481

    • Search Google Scholar
    • Export Citation
  • Porrero, M. C., Hasman, H., Vela, A. I., Fernández-Garayzábal, J. F., Domínguez, L. and Aarestrup, F. M. (2012): Clonal diversity of Staphylococcus aureus originating from the small ruminants goats and sheep. Vet. Microbiol. 156, 157161. doi: 10.1016/j.vetmic.2011.10.015

    • Search Google Scholar
    • Export Citation
  • Rodrigues, A. C., Belas, A., Marques, C., Cruz, L., Gama, L. T. and Pomba, C. (2018): Risk factors for nasal colonization by methicillin-resistant staphylococci in healthy humans in professional daily contact with companion animals in Portugal. Microbial Drug Res. 24, 434446. doi: 10.1089/mdr.2017.0063

    • Search Google Scholar
    • Export Citation
  • Sahibzada, S., Abraham, S., Coombs, G. W., Pang, S., Hernández-Jover, M., Jordan, D. and Heller, J. (2017): Transmission of highly virulent community-associated MRSA ST93 and live-stock-associated MRSA ST398 between humans and pigs in Australia. Scientific Reports 7, 5273. doi: 10.1038/s41598-017-04789-0

    • Search Google Scholar
    • Export Citation
  • Sato, T., Usui, M., Maetani, S. and Tamura, Y. (2018): Prevalence of methicillin-resistant Staphylococcus aureus among veterinary staff in small animal hospitals in Sapporo, Japan, between 2008 and 2016: A follow up study. J. Infect. Chemother. 24, 588591. doi: 10.1016/j.jiac.2018.01.016

    • Search Google Scholar
    • Export Citation
  • Shore, A. C. and Coleman, D. C. (2013): Staphylococcal cassette chromosome mec: recent advances and new insights. Int. J. Med. Microbiol. 303, 350359. doi: 10.1016/j.ijmm.2013.02.002

    • Search Google Scholar
    • Export Citation
  • Smith, C. L., Klco, S. R. and Cantor, C. R. (1988): Pulsed-field gel electrophoresis and the technology of large DNA molecules. In: Davies, K. E. (ed.) Genome Analysis: A Practical Approach. Oxford IRL Press, Oxford, United Kingdom. pp. 4172.

    • Search Google Scholar
    • Export Citation
  • Sun, J., Yang, M., Sreevatsan, S., Bender, J. B., Singer, R. S., Knutson, T. P., Marthaler, D. G. and Davies, P. R. (2017): Longitudinal study of Staphylococcus aureus colonization and infection in a cohort of swine veterinarians in the United States. BMC Infec. Dis. 17, 690. doi: 10.1186/s12879-017-2802-1

    • Search Google Scholar
    • Export Citation
  • Tenover, F. C., Arbeit, R. D., Goering, R. V., Mickelsen, P. A., Murray, B. E., Persing, D. H. and Swaminathan, B. (1995): Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33, 22332239.

    • Search Google Scholar
    • Export Citation
  • Traversa, A., Gariano, G. R., Gallina, S., Bianchi, D. M., Orusa, R., Domenis, L., Cavallerio, P., Fossati, L., Serra, R. and Decastelli, L. (2015): Methicillin resistance in Staphylococcus aureus strains isolated from food and wild animal carcasses in Italy. Food Microbiol. 52, 154158. doi: 10.1016/j.fm.2015.07.012

    • Search Google Scholar
    • Export Citation
  • Vanderhaeghen, W., Piepers, S., Leroy, F., Van Coillie, E., Haesebrouck, F. and De Vliegher, S. (2014): Effect, persistence, and virulence of coagulase-negative Staphylococcus species associated with ruminant udder health. J. Dairy Sci. 97, 52755293. doi: 10.3168/jds.2013-7775

    • Search Google Scholar
    • Export Citation
  • Zhang, Y., Agidi, S. and LeJeune, J. T. (2009): Diversity of staphylococcal cassette chromosome in coagulase-negative staphylococci from animal sources. J. Appl. Microbiol. 107, 1375-1383. doi: 10.1111/j.1365-2672.2009.04322x

    • Search Google Scholar
    • Export Citation

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