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  • 1 Université Laval, Quebec City, G1V 0A6 Quebec, Canada
  • | 2 Pasteur Institute of Tunis, Tunisia
  • | 3 Universidad de La Rioja, Logroño, Spain
  • | 4 Université de Tunis El Manar, Tunis, Tunisie
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The spread of antimicrobial-resistant bacteria in wildlife must be viewed as a major concern with serious implications for human and animal health. Escherichia coli and enterococcal isolates were recovered from faecal samples of 49 wild rabbits (Oryctolagus cuniculus) on specific media and were characterised using biochemical and molecular tests. For all isolates, antimicrobial susceptibility testing was performed, and resistance genes were detected by PCR. Molecular typing of isolates was carried out by pulsed-field gel-electrophoresis, and E. coli strains were also tested for the presence of intimin (eae) gene characteristic of rabbit enteropathogenic E. coli. A total of 34 E. coli and 36 enterococci [E. hirae (52.8%) and E. faecalis (47.2%)] were obtained. For E. coli, resistance to tetracycline (94%), streptomycin (62%), ciprofloxacin (47%), trimethoprim-sulphamethoxazole (35%) and chloramphenicol (6%) was observed. Resistance to third-generation cephalosporins was detected in one E. coli strain that carried the blaCMY-2 and blaTEM-1 genes. Class 1 integrons were detected in eight isolates. For enterococci, resistance to tetracycline (63.9%), erythromycin (30.5%), streptomycin (18.2%), and chloramphenicol (5.5%) was detected. The tet(M)+tet(L), erm(B) and ant (6)-Ia genes were identified in thirteen, seven and three resistant Enterococcus strains, respectively. Molecular typing showed a high diversity among our strains. Wild rabbits could represent a reservoir of E. coli, and enterococci carrying antimicrobial resistance genes and E. coli additionally carrying the eae gene of enteropathogenic pathotypes could both contaminate the environment. our finding seems to represent the first report of eae-positive E. coli in wild rabbits.

  • Allen, H. K., Donato, J., Wang, H. H., Cloud-Hansen, K. A., Davies, J. and Handelsman, J. (2010): Call of the wild: antibiotic resistance genes in natural environments. Nat. Rev. Microbiol. 8, 251259.

    • Search Google Scholar
    • Export Citation
  • Alonso, C. A., Mora, A., Díaz, D., Blanco, M., González-Barrio, D., Ruiz-Fons, F., Simón, C., Blanco, J. and Torres, C. (2017): Occurrence and characterization of stx and/or eae-positive Escherichia coli isolated from wildlife, including a typical EPEC strain from a wild boar. Vet. Microbiol. 207, 6973.

    • Search Google Scholar
    • Export Citation
  • Ben Said, L., Jouini, A., Alonso, C. A., Klibi, N., Dziri, R., Boudabous, A., Ben Slama, K. and Torres, C. (2016): Characteristics of extended-spectrum β-lactamase (ESBL) and pAmpC beta-lactamase-producing Enterobacteriaceae of water samples in Tunisia. Sci. Total Environ. 550, 11031109.

    • Search Google Scholar
    • Export Citation
  • Ben Said, L., Klibi, N., Dziri, R., Borgo, F., Boudabous, A., Ben Slama, K. and Torres, C. (2015): Prevalence, antimicrobial resistance and genetic lineages of Enterococcus spp. from vegetable food, soil and irrigation water in farm environments in Tunisia. J. Sci. Food Agric. 96, 16271633.

    • Search Google Scholar
    • Export Citation
  • Ben Sallem, R., Ben Slama, K., Sáenz, Y., Rojo-Bezares, B., Estepa, V., Jouini, A., Gharsa, H., Klibi, N., Boudabous, A. and Torres, C. (2012): Prevalence and characterization of extended-spectrum beta-lactamase (ESBL)- and CMY-2-producing Escherichia coli isolates from healthy food-producing animals in Tunisia. Foodborne Pathog. Dis. 9, 11371142.

    • Search Google Scholar
    • Export Citation
  • Ben Slama, K., Jouini, A., Ben Sallem, R., Somalo, S., Sáenz, Y., Estepa, V., Boudabous, A. and Torres, C. (2010): Prevalence of broad-spectrum cephalosporin-resistant Escherichia coli isolates in food samples in Tunisia and characterization of integrons and antimicrobial resistance mechanisms implicated. Int. J. Food. Microbiol. 137, 281286.

    • Search Google Scholar
    • Export Citation
  • Butaye, P., Devriese, L. A. and Haesebrouck, F. (2001): Differences in antibiotic resistance patterns of Enterococcus faecalis and Enterococcus faecium strains isolated from farm and pet animals. Antimicrob. Agents Chemother. 45, 13741378.

    • Search Google Scholar
    • Export Citation
  • CLSI (2017): Performance Standards for Antimicrobial Susceptibility Testing. Twenty-fifth Informational Supplement. CLSI Document, M100S25.

    • Search Google Scholar
    • Export Citation
  • Cole, D., Drum, D. J. V., Stallknecht, D. E., White, D. G., Lee, M. D., Ayers, S., Sobsey, M. and Maurer, J. J. (2005): Free-living Canada geese and antimicrobial resistance. Emerg. Infect. Dis. 11, 935938.

    • Search Google Scholar
    • Export Citation
  • Costa, D., Poeta, P., Sáenz, Y., Vinué, L., Coelho, A., Matos, M., Rojo-Bezares, B., Rodrigues, J. and Torres, C. (2008): Mechanisms of antibiotic resistance in Escherichia coli isolates recovered from wild animals. Microb. Drug Resist. 14, 7177.

    • Search Google Scholar
    • Export Citation
  • Da Costa, P. M., Loureiro, L. and Matos, A. J. F. (2013): Transfer of multidrug resistant bacteria between intermingled ecological niches: the interface between humans, animals and the environment. Int. J. Environ. Res. Public Health 10, 278294.

    • Search Google Scholar
    • Export Citation
  • Dotto, G., Giacomelli, M., Grilli, G., Ferrazzi, V., Carattoli, A., Fortini, D. and Piccirillo, A. (2014): High prevalence of oqxAB in Escherichia coli isolates from domestic and wild lagomorphs in Italy. Microb. Drug Resist. 20, 118123.

    • Search Google Scholar
    • Export Citation
  • Elhani, D., Klibi, N., Dziri, R., Ben Hassan, M., Asli, M. S., Ben Said, L., Mahjoub, A., Ben Slama, K., Jemli, B., Bellaj, R., Barguellil, F. and Torres, C. (2014): vanA-containing E. faecium isolates of clonal complex CC17 in clinical and environmental samples in a Tunisian hospital. Diagn. Microbiol. Infect. Dis. 79, 6063.

    • Search Google Scholar
    • Export Citation
  • Figueiredo, N., Radhouani, H., Gonçalves, A., Rodrigues, J., Carvalho, C., Igrejas, G. and Poeta, P. (2009): Genetic characterization of vancomycin-resistant enterococci isolates from wild rabbits. J. Basic. Microbiol. 49, 491494.

    • Search Google Scholar
    • Export Citation
  • Franz, C. M. A. P., Holzapfel, W. H. and Stiles, M. E. (1999): Enterococci at the crossroads of food safety? Int. J. Food. Microbiol. 47, 124.

    • Search Google Scholar
    • Export Citation
  • Guardabassi, L., Schwarz, S. and Lloyd, D. H. (2004): Pet animals as reservoirs of antimicrobial-resistant bacteria. J. Antimicrob. Chemother. 54, 321332.

    • Search Google Scholar
    • Export Citation
  • Guerrero-Ramos, E., Cordero, J., Molina-González, D., Poeta, P., Igrejas, G., Alonso-Calleja, C. and Capita, R. (2016): Antimicrobial resistance and virulence genes in enterococci from wild game meat in Spain. Food. Microbiol. 53, 156164.

    • Search Google Scholar
    • Export Citation
  • Huys, G., D’Haene, G. K., Collard, J. M. and Swings, J. (2004): Prevalence and molecular characterization of tetracycline resistance in Enterococcus isolates from food. Appl. Environ. Microbiol. 70, 15551562.

    • Search Google Scholar
    • Export Citation
  • Jackson, C. R., Fedorka-Cray, P. J., Davis, J. A., Barrett, J. B., Brousse, J. H., Gustafson, J. and Kucher, M. (2010): Mechanisms of antimicrobial resistance and genetic relatedness among enterococci isolated from dogs and cats in the United States. J. Appl. Microbiol. 108, 21712179.

    • Search Google Scholar
    • Export Citation
  • Jouini, A., Ben Slama, K., Saenz, Y., Klibi, N., Costa, D., Vinué, L., Zarazaga, M., Boudabous, A. and Torres, C. (2009): Detection of multiple-antimicrobial resistance and characterization of the implicated genes in Escherichia coli isolates from food of animal origin in Tunis. J. Food Prot. 72, 10821088.

    • Search Google Scholar
    • Export Citation
  • Klibi, N., Aouini, R., Borgo, F. C., Ben Said, L., Ferrario, C., Dziri, R., Boudabous, A., Torres, C. and Ben Slama, K. (2015a): Antibiotic resistance and virulence of faecal enterococci isolated from food-producing animals in Tunisia. Ann. Microbiol. 65, 18.

    • Search Google Scholar
    • Export Citation
  • Klibi, N., Ben Amor, I., Rahmouni, M., Dziri, R., Gtari, D., Ben Said, L., Lozano, C., Boudabous, A., Ben Slama, K., Mansouri, R. and Torres, C. (2015b): Diversity of species and antibiotic resistance among fecal enterococci from wild birds in Tunisia. Detection of vanA-containing Enterococcus faecium isolates. Eur. J. Wildl. Res. 61, 319323.

    • Search Google Scholar
    • Export Citation
  • Klibi, N., Ben Lagha, A., Ben Slama, K., Boudabous, A. and Torres, C. (2013a): Faecal enterococci from camels in Tunisia: species, antibiotic resistance and virulent genes. Vet. Rec. 172, 213.

    • Search Google Scholar
    • Export Citation
  • Klibi, N., Ben Said, L., Jouini, A., Ben Slama, K., López, M., Ben Sallem, R., Boudabous, A. and Torres, C. (2013b): Species distribution, antibiotic resistance and virulence traits in enterococci from meat in Tunisia. Meat Sci. 93, 675680.

    • Search Google Scholar
    • Export Citation
  • Klibi, N., Ben Slama, K., Masmoudi, A., Gharbi, S., Ruiz-Larrea, F., Fendri, C., Boudabous, A. and Torres, C. (2006): Diversity of structures carrying the aac(6’)-aph(2”) gene in clinical E. faecalis and E. faecium strains isolated in Tunisia. J. Chemother. 18, 353359.

    • Search Google Scholar
    • Export Citation
  • Linaje, R., Coloma, M. D., Perez-Martinez, G. and Zuniga, M. (2004): Characterization of faecal enterococci from rabbits for the selection of probiotic strains. J. Appl. Microbiol. 96, 761771.

    • Search Google Scholar
    • Export Citation
  • López, M., Sáenz, Y., Rojo-Bezares, B., Martínez, S., del Campo, R., Ruiz-Larrea, F., Zarazaga, M. and Torres, C. (2009): Detection of vanA and vanB2-containing enterococci from food samples in Spain, including Enterococcus faecium strains of CC17 and the new singleton ST425. Int. J. Food Microbiol. 133, 172178.

    • Search Google Scholar
    • Export Citation
  • Marinho, C., Igrejas, G., Gonçalves, A., Silva, N., Santos, T., Monteiro, R., Gonçalves, D., Rodrigues, T. and Poeta, P. (2014): Azorean wild rabbits as reservoirs of antimicrobial resistant Escherichia coli. Anaerobe 30, 116119.

    • Search Google Scholar
    • Export Citation
  • Poeta, P., Costa, D., Rodrigues, J. and Torres, C. (2006): Antimicrobial resistance and the mechanisms implicated in faecal enterococci from healthy humans, poultry and pets in Portugal. Int. J. Antimicrob. Agents 27, 131137.

    • Search Google Scholar
    • Export Citation
  • Poeta, P., Costa, D., Saenz, Y., Klibi, N., Ruiz-Larrea, F., Rodrigues, J. and Torres, C. (2005): Characterization of antibiotic resistance genes and virulence factors in faecal enterococci of wild animals in Portugal. J. Vet. Med. 52, 396402.

    • Search Google Scholar
    • Export Citation
  • Ritchie, J. M., Thorpe, C. M., Rogers, A. B. and Waldor, M. K. (2003): Critical roles for stx2, eae, and tir in enterohemorrhagic Escherichia coli-induced diarrhea and intestinal inflammation in infant rabbits. Infect. Immun. 71, 71297139.

    • Search Google Scholar
    • Export Citation
  • Sáenz, Y., Brinăs, L., Domínguez, E., Ruiz, J., Zarazaga, M., Jordi, V. and Torres, C. (2004): Mechanisms of resistance in multiple-antibiotic-resistant Escherichia coli strains of human, animal and food origins. Antimicrob. Agents Chemother. 48, 39961001.

    • Search Google Scholar
    • Export Citation
  • Sáenz, Y., Zarazaga, M., Briňas, L., Lantero, M., Ruiz-Larrea, F. and Torres, C. (2001): Antibiotic resistance in Escherichia coli isolates obtained from animals, foods and humans in Spain. Int. J. Antimicrob. Agents 18, 353358.

    • Search Google Scholar
    • Export Citation
  • Santos, T., Silva, N., Igrejas, G., Rodrigues, P., Micael, J., Rodrigues, T., Resende, R., Gonçalves, A., Marinho, C., Gonçalves, D., Cunha, R. and Poeta, P. (2013): Dissemination of antibiotic resistant Enterococcus spp. and Escherichia coli from wild birds of Azores Archipelago. Anaerobe 24, 2531.

    • Search Google Scholar
    • Export Citation
  • Sayah, R. S., Kaneene, J. B., Johnson, Y. and Miller, R. (2005): Patterns of antimicrobial resistance observed in Escherichia coli isolates obtained from domestic- and wild-animal fecal samples, human septage, and surface water. Appl. Environ. Microbiol. 71, 13941404.

    • Search Google Scholar
    • Export Citation
  • Silva, N., Igrejas, G., Figueiredo, N., Gonçalves, A., Radhouani, H., Rodrigues, J. and Poeta, P. (2010): Molecular characterization of antimicrobial resistance in enterococci and Escherichia coli isolates from European wild rabbit (Oryctolagus cuniculus). Sci. Total Environ. 408, 48714876.

    • Search Google Scholar
    • Export Citation
  • Teshager, T., Herrero, I. A., Porrero, M. C., Garde, J., Moreno, M. A. and Domĕnguez, L. (2000): Surveillance of antimicrobial resistance in Escherichia coli strains isolated from pigs at Spanish slaughterhouses. Int. J. Antimicrob. Agents 15, 137142.

    • Search Google Scholar
    • Export Citation
  • Torres, C., Tenorio, C., Portillo, A., Garcia, M., Martínez, C., del Campo, R., Ruiz-Larrea, F. and Zarazaga, M. (2003): Intestinal colonization by vanA or vanB2-containing enterococcal isolates of healthy animals in Spain. Microb. Drug Resist. 9, 4752.

    • Search Google Scholar
    • Export Citation
  • Turabelidze, D., Kotetishvili, M., Kreger, A., Morris, J. G. and Sulakvelidze, A. (2000): Improved pulsed-field gel electrophoresis for typing vancomycin-resistant enterococci. J. Clin. Microbiol. 38, 42424245.

    • Search Google Scholar
    • Export Citation
  • Van den Bogaard, A. E. and Stobberingh, E. E. (2000): Epidemiology of resistance to antibiotics. Links between animals and humans. Int. J. Antimicrob. Agents 14, 327335.

    • Search Google Scholar
    • Export Citation

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Senior editors

Editor-in-Chief: Mária BENKŐ

Managing Editor: András SZÉKELY

Editorial Board

  • Béla DÉNES (National Food Chain Safety Office, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (National Agricultural Innovation Centre, Herceghalom, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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2020  
Total Cites 987
WoS
Journal
Impact Factor
0,955
Rank by Veterinary Sciences 101/146 (Q3)
Impact Factor  
Impact Factor 0,920
without
Journal Self Cites
5 Year 1,164
Impact Factor
Journal  0,57
Citation Indicator  
Rank by Journal  Veterinary Sciences 93/166 (Q3)
Citation Indicator   
Citable 49
Items
Total 49
Articles
Total 0
Reviews
Scimago 33
H-index
Scimago 0,395
Journal Rank
Scimago Veterinary (miscellaneous) Q2
Quartile Score  
Scopus 355/217=1,6
Scite Score  
Scopus General Veterinary 73/183 (Q2)
Scite Score Rank  
Scopus 0,565
SNIP  
Days from  145
submission  
to acceptance  
Days from  150
acceptance  
to publication  
Acceptance 19%
Rate

 

2019  
Total Cites
WoS
798
Impact Factor 0,991
Impact Factor
without
Journal Self Cites
0,897
5 Year
Impact Factor
1,092
Immediacy
Index
0,119
Citable
Items
59
Total
Articles
59
Total
Reviews
0
Cited
Half-Life
9,1
Citing
Half-Life
9,2
Eigenfactor
Score
0,00080
Article Influence
Score
0,253
% Articles
in
Citable Items
100,00
Normalized
Eigenfactor
0,09791
Average
IF
Percentile
42,606
Scimago
H-index
32
Scimago
Journal Rank
0,372
Scopus
Scite Score
335/213=1,6
Scopus
Scite Score Rank
General Veterinary 62/178 (Q2)
Scopus
SNIP
0,634
Acceptance
Rate
18%

 

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Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation
1951
Publication
Programme
2020 Volume 68
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

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