Acta Veterinaria Hungarica
Volume/Issue:
Volume 68: Issue 4
Expression of the Wilms’ tumour gene and its association with PPARβ/δ in healthy skin and melanoma of horses
Authors:
Itzel Y. Rangel-Sánchez Department of Genetics, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Av. Francisco Villa s/n, Ex Hacienda el Canadá, 66050 Gral. Escobedo, NL, Mexico

Search for other papers by Itzel Y. Rangel-Sánchez in
Current site
Google Scholar
PubMed Close
,
Daniel Salas-Treviño Department of Histology, Faculty of Medicine, Autonomous University of Nuevo León, Mexico

Search for other papers by Daniel Salas-Treviño in
Current site
Google Scholar
PubMed Close
,
Adolfo Soto-Domínguez Department of Histology, Faculty of Medicine, Autonomous University of Nuevo León, Mexico

Search for other papers by Adolfo Soto-Domínguez in
Current site
Google Scholar
PubMed Close
,
Oscar I. Garza-Rodríguez Department of Genetics, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Av. Francisco Villa s/n, Ex Hacienda el Canadá, 66050 Gral. Escobedo, NL, Mexico

Search for other papers by Oscar I. Garza-Rodríguez in
Current site
Google Scholar
PubMed Close
,
Odila Saucedo-Cárdenas Department of Histology, Faculty of Medicine, Autonomous University of Nuevo León, Mexico
Northeast Biomedical Research Centre (CIBIN) of the IMSS, Monterrey, NL, Mexico

Search for other papers by Odila Saucedo-Cárdenas in
Current site
Google Scholar
PubMed Close
,
Pablo Zapata-Benavides Laboratory of Immunology and Virology, Faculty of Biological Sciences, Autonomous University of Nuevo León, Mexico

Search for other papers by Pablo Zapata-Benavides in
Current site
Google Scholar
PubMed Close
,
Juan J. Zarate-Ramos Department of Parasitology, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Mexico

Search for other papers by Juan J. Zarate-Ramos in
Current site
Google Scholar
PubMed Close
,
Sibilina Cedillo-Rosales Department of Virology, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Mexico

Search for other papers by Sibilina Cedillo-Rosales in
Current site
Google Scholar
PubMed Close
, and
Diana E. Zamora-Ávila Department of Genetics, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Av. Francisco Villa s/n, Ex Hacienda el Canadá, 66050 Gral. Escobedo, NL, Mexico

Search for other papers by Diana E. Zamora-Ávila in
Current site
Google Scholar
PubMed Close
https://orcid.org/0000-0002-2225-0495
Pages:
374–379
Online Publication Date:
15 Jan 2021
Publication Date:
19 Apr 2021
Article Category:
Research Article
DOI:
https://doi.org/10.1556/004.2020.00045
Keywords:
skin; melanoma; equine; WT1 gene; PPARβ/δ gene
Restricted access

Abstract

The Wilms’ tumour gene (WT1) has previously been described as an oncogene in several neoplasms of humans, including melanoma, and its expression increases cancer cell proliferation. Recent reports associate the expression of the PPARβ/δ gene (peroxisome proliferator-activated receptor beta/delta) with the downregulation of WT1 in human melanoma and murine melanoma cell lines. The aim of this work was to analyse the expression of WT1 and its association with PPARβ/δ in samples of healthy and melanoma-affected skin of horses by immunohistochemistry. WT1 protein expression was detected in healthy skin, mainly in the epidermis, hair follicle, sebaceous gland and sweat gland, while no expression was observed in equine melanoma tissues. Moreover, it was observed that PPARβ/δ has a basal expression in healthy skin and that it is overexpressed in melanoma. These results were confirmed by a densitometric analysis, where a significant increase of the WT1-positive area was observed in healthy skin (128.66 ± 19.84 pixels 106) compared with that observed in melanoma (1.94 ± 0.04 pixels 106). On the other hand, a positive area with an expression of PPARβ/δ in healthy skin (214.94 ± 11.85 pixels 106) was significantly decreased compared to melanoma (624.86 ± 181.93 pixels 106). These data suggest that there could be a regulation between WT1 and PPARβ/δ in this disease in horses.

  • Bellone, R. R. (2010): Pleiotropic effects of pigmentation genes in horses. Anim. Genet. 41, 100–110.

  • Brouillette, J. A., Andrew, J. R. and Venta, P. J. (2000): Estimate of nucleotide diversity in dogs with a pool-and-sequence method. Mamm. Genome 11, 1079–1086.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Burden, K. (2011): Melanomas and their effect on the grey horses. Young Sci. J. 10, 75–81.

  • Dunning, K. R., Anastasi, M. R., Zhang, V. J., Russell, D. L. and Robker, R. L. (2014): Regulation of fatty acid oxidation in mouse cumulus-oocyte complexes during maturation and modulation by PPAR agonists. PLoS One 9, e87327.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Fleury, C., Bérard, F., Leblond, A., Faure, C., Ganem, N. and Thomas, L. (2000): The study of cutaneous melanomas in Camargue-type gray-skinned horses (2): Epidemiological survey. Pigm. Cell Res. 13, 47–51.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Garrido Ruiz, M. C., Rodríguez Peralto, J. L. and Carrillo Gijón, R. (2010): Comparison of the Protein Expression Profile Between Nevi and Melanoma [in Spanish]. Doctoral Thesis, Universidad Complutense de Madrid Facultad de Medicina, Departamento de Anatomía Patológica, Madrid, Spain. pp. 1–235. ISBN: .

    • Search Google Scholar
    • Export Citation

  • Garza-Rodríguez, O. (2015): Analysis of the Expression of WT1 Gene (Wilm’s tumor) in Equine Melanoma [in Spanish]. MSc Thesis, Universidad Autónoma de Nuevo León, Monterrey, NL.

    • Search Google Scholar
    • Export Citation

  • Ham, S. A., Yoo, T., Hwang, J. S., Kang, E. S., Lee, W. J., Paek, K. S., Park, C., Kim, J.-H., Do, J. T., Lim, D.-S. and Seo, H. G. (2014): Ligand-activated PPARδ modulates the migration and invasion of melanoma cells by regulating Snail expression. Am. J. Cancer Res. 4, 674–682.

    • Search Google Scholar
    • Export Citation

  • Hira, V. V. V., Loncq de Jong, A., Ferro, K., Khurshed, M., Molenaar, R. J. and van Noorden, C. J. F. (2019): Comparison of different methodologies and cryostat versus paraffin sections for chromogenic immunohistochemistry. Acta Histochem.. 121, 125–134.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Lee, C. H., Olson, P., Hevener, A., Mehl, I., Chong, L. W., Olefsky, J. M., Gonzalez, F. J., Ham, J., Kang, H., Peters, J. M. and Evans, R. M. (2006): PPARδ regulates glucose metabolism and insulin sensitivity. Proc. Natl Acad. Sci. U.S.A. 103, 3444–3449.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Michiels, J. F., Perrin, C., Leccia, N., Massi, D., Grimaldi, P. and Wagner, N. (2010): PPARβ activation inhibits melanoma cell proliferation involving repression of the Wilms’ tumor suppressor WT1. Pflügers Arch. – Eur. J. Physiol. 459, 689–703.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Mrowka, C. and Schedl, A. (2000): Wilms’ tumor suppressor gene WT1: From structure to renal pathophysiologic features. J. Am. Soc. Nephrol. 11, S106–S115.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Nakatsuka, S., Oji, Y., Horiuchi, T., Kanda, T., Kitagawa, M., Takeuchi, T., Kawano, K., Kuwae, Y., Yamauchi, A., Okumura, M., Kitamura, Y., Oka, Y., Kawase, I., Sugiyama, H. and Aozasa, K. (2006): Immunohistochemical detection of WT1 protein in a variety of cancer cells. Mod. Pathol. 19, 804–814.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Rodríguez-Martín, M. (2008): Targeting the PPARs. Med. Cutan. Iber. Am. (4) 161–163.

  • Schaffer, P. A., Wobeser, B., Martin, L. E., Dennis, M. M. and Duncan, C. G. (2012): Cutaneous neoplastic lesions of equids in the central United States and Canada: 3,351 biopsy specimens from 3,272 equids (2000–2010). J. Am. Vet. Med. Assoc. 242, 99–104.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Seltenhammer, M. H., Sundström, E., Meisslitzer-Ruppitsch, C., Cejka, P., Kosiuk, J., Neumüller, J., Almeder, M., Majdic, O., Steinberger, P., Losert, U. M., Stöckl, J., Andersson, L., Sölkner, J., Vetterlein, M. and Golovko, A. (2014): Establishment and characterization of a primary and a metastatic melanoma cell line from Grey horses. In Vitro Cell. Dev. Biol. Anim. 50, 56–65.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Sutton, R. H. and Coleman, G. T. (1997): Melanoma and the greying horse: A study of the grey horse melanoma, with special reference to prevalence, tumor structure and biology and associated pigment metabolism abnormalities: A report for the rural industries research development corporation. Rural Ind. Res. Develop. Corp. 1–23.

    • Search Google Scholar
    • Export Citation

  • Tyagi, S., Gupta, P., Saini, A. S., Kaushal, C. and Sharma, S. (2011): The peroxisome proliferator-activated receptor: A family of nuclear receptors role in various diseases. J. Adv. Pharm. Technol. Research (JAPTR) 2, 236–240.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Valentine, B. A. (2006): Survey of equine cutaneous neoplasia in the Pacific Northwest. J. Vet. Diagn. Invest. 18, 123–126.

  • Wagner, N., Panelos, J., Massi, D. and Wagner, K. D. (2008): The Wilms’ tumor suppressor WT1 is associated with melanoma proliferation. Pflugers Arch. – Eur. J. Physiol. 455, 839–847.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Wilsher, M. and Cheerala, B. (2007): WT1 as a complementary marker of malignant melanoma: an immunohistochemical study of whole sections. Histopathology 51, 605–610.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Young, G., Toretsky, J. A., Campbell, A. B. and Eskenazi, A. E. (2000): Recognition of common childhood malignancies. Am. Fam. Physician. 61, 2144–2154.

    • Search Google Scholar
    • Export Citation

  • Zamora-Avila, D. E., Franco-Molina, M. A., Trejo-Avila, L. M., Rodríguez-Padilla, C., Reséndez-Pérez, D. and Zapata-Benavides, P. (2007): RNAi silencing of the WT1 gene inhibits cell proliferation and induces apoptosis in the B16F10 murine melanoma cell line. Melanoma Res. 17, 341–348.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Zamora-Avila, D. E., Zapata-Benavides, P., Franco-Molina, M. A., Saavedra-Alonso. S., Trejo-Avila, L. M., Reséndez-Pérez, D., Méndez-Vázquez, J. L., Isaias-Badillo, J. and Rodríguez-Padilla, C. (2009): WT1 gene silencing by aerosol delivery of PEI-RNAi complexes inhibits B16-F10 lung metastases growth. Canc. Gene Ther. 16, 892–899.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Collapse
  • Expand
Cover Acta Veterinaria Hungarica
Acta Veterinaria Hungarica
Print ISSN:
0236-6290
Online ISSN:
1588-2705

Senior editors

Editor-in-Chief: Ferenc BASKA

Editorial assistant: Szilvia PÁLINKÁS

 

Editorial Board

  • Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
  • Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • János GÁL (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA

University of Veterinary Medicine,

H-1078 Budapest, István utca 2., Hungary

Phone: (36 20) 560 4183 (ed.-in-chief) or (36 1) 478 4100/8430 (editor)

E-mail: acta.veterinaria@univet.hu (ed.-in-chief)

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture, Biology and Environmental Sciences
  • Elsevier Science Navigator
  • Focus On: Veterinary Science and Medicine
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

2023  
Web of Science  
Journal Impact Factor 0.7
Rank by Impact Factor Q3 (Veterinary Sciences)
Journal Citation Indicator 0.4
Scopus  
CiteScore 1.8
CiteScore rank Q2 (General Veterinary)
SNIP 0.39
Scimago  
SJR index 0.258
SJR Q rank Q3

Acta Veterinaria Hungarica
Publication Model Hybrid
Submission Fee none
Article Processing Charge 1100 EUR/article
Printed Color Illustrations 40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2025 Online subsscription: 832 EUR / 916 USD
Print + online subscription: 960 EUR / 1054 USD
Subscription Information Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation		 1951
Volumes
per Year		 1
Issues
per Year		 4
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2024 74 0 0
Sep 2024 76 0 0
Oct 2024 229 0 0
Nov 2024 61 0 0
Dec 2024 64 0 0
Jan 2025 44 1 2
Feb 2025 0 0 0