Acta Veterinaria Hungarica
Volume/Issue:
Volume 69: Issue 2
Retrospective immunohistochemical investigation on dolphin morbillivirus infection by comparing the performance of heterologous monoclonal and polyclonal antibodies – Short communication
Authors:
Federica Giorda Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, via Bologna 148, 10154, Torino, Italy
Institute of Animal Health, University of Las Palmas de Gran Canaria, Arucas, Las Palmas, Spain

Search for other papers by Federica Giorda in
Current site
Google Scholar
PubMed Close
,
Giovanni Di Guardo Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy

Search for other papers by Giovanni Di Guardo in
Current site
Google Scholar
PubMed Close
,
Katia Varello Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, via Bologna 148, 10154, Torino, Italy

Search for other papers by Katia Varello in
Current site
Google Scholar
PubMed Close
,
Alessandra Pautasso Department of Prevention, Local Veterinary Services (ASL1 Imperiese), Bussana di Sanremo, Imperia, Italy

Search for other papers by Alessandra Pautasso in
Current site
Google Scholar
PubMed Close
,
Eva Sierra Institute of Animal Health, University of Las Palmas de Gran Canaria, Arucas, Las Palmas, Spain

Search for other papers by Eva Sierra in
Current site
Google Scholar
PubMed Close
,
Maria Domenica Pintore Department of Prevention, Local Veterinary Services (ASL VCO), Domodossola (VB), Italy

Search for other papers by Maria Domenica Pintore in
Current site
Google Scholar
PubMed Close
,
Carla Grattarola Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, via Bologna 148, 10154, Torino, Italy

Search for other papers by Carla Grattarola in
Current site
Google Scholar
PubMed Close
,
Erika Molica Colella Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Brescia, Italy

Search for other papers by Erika Molica Colella in
Current site
Google Scholar
PubMed Close
,
Enrica Berio Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, via Bologna 148, 10154, Torino, Italy

Search for other papers by Enrica Berio in
Current site
Google Scholar
PubMed Close
,
Maria Goria Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, via Bologna 148, 10154, Torino, Italy

Search for other papers by Maria Goria in
Current site
Google Scholar
PubMed Close
,
Elena Bozzetta Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, via Bologna 148, 10154, Torino, Italy

Search for other papers by Elena Bozzetta in
Current site
Google Scholar
PubMed Close
,
Cristina Casalone Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, via Bologna 148, 10154, Torino, Italy

Search for other papers by Cristina Casalone in
Current site
Google Scholar
PubMed Close
, and
Barbara Iulini Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, via Bologna 148, 10154, Torino, Italy

Search for other papers by Barbara Iulini in
Current site
Google Scholar
PubMed Close
https://orcid.org/0000-0002-6037-5184
Pages:
204–210
Online Publication Date:
23 Jul 2021
Publication Date:
31 Jul 2021
Article Category:
Brief Report
DOI:
https://doi.org/10.1556/004.2021.00028
Keywords:
cetaceans; dolphin morbillivirus; immunohistochemistry; monoclonal antibody; polyclonal antibody
Restricted access

Abstract

Dolphin morbillivirus (DMV) is a pathogen of great concern in free-ranging cetaceans. Confirmation and staging of morbillivirus infections rely on histology and immunohistochemistry (IHC), following molecular detection. As at the present time no specific antibodies (Abs) against DMV are available, two heterologous Abs have been used worldwide for the examinations of morbillivirus infections of cetaceans. One is a monoclonal Ab (MoAb) prepared against the N protein of canine distemper virus (CDV), whereas the other is a polyclonal Ab raised in rabbits against rinderpest virus (RPV). Both Abs are known to show cross-reactivity with DMV. In this study we compared the labelling quality and the neuroanatomical distribution of staining with these two Abs by means of IHC analysis. To this end, serial sections of the target organs from ten free-ranging stranded cetaceans, previously diagnosed as being infected with DMV by PCR and/or serology, were subjected to IHC. The brain, lungs and lymph nodes of one animal were found to be positive with both Abs. From two other animals, the brain and the spleen, respectively, tested positive only with the polyclonal Ab. In the positive brain tissues, multifocal immunostaining was observed, with similar staining location and extent, with the two antibodies tested. Our results suggest that the polyclonal anti-RPV Ab might have a stronger binding activity to DMV than the anti-CDV MoAb. Nevertheless, the elaboration and use of specific anti-DMV Abs might be essential to guarantee conclusive results in diagnostic and pathogenetic investigations.

  • Amude, A. M., Alfieri, A. F. and Alfieri, A. A. (2010): Use of immunohistochemistry and molecular assays such as RT-PCR for precise post mortem diagnosis of distemper-related encephalitis. In: Méndez-Vilas, A. (ed.) Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, Formatex, Spain. pp. 1539–1545.

    • Search Google Scholar
    • Export Citation

  • Di Guardo, G., Proietto, U., Di Francesco, C. E., Marsilio, F., Zaccaroni, A., Scaravelli, D., Mignone, W., Garibaldi, F., Kennedy, S., Forster, F., Iulini, B., Bozzetta, E. and Casalone, C. (2010): Cerebral toxoplasmosis in striped dolphins (Stenella coeruleoalba) stranded along the Ligurian Sea coast of Italy. Vet. Pathol. 47, 245–253.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Díaz-Delgado, J., Groch, K. R., Sierra, E., Sacchini, S., Zucca, D., Quesada-Canales, Ó., Arbelo, M., Fernández, A., Santos, E., Ikeda, J., Carvalho, R., Azevedo, A. F., Lailson-Brito, J. Jr., Flach, L., Ressio, R., Kanamura, C. T., Sansone, M., Favero, C., Porter, B. F., Centelleghe, C., Mazzariol, S., Di Renzo, L., Di Francesco, G., Di Guardo, G. and Catão- Dias, J. L. (2019): Comparative histopathologic and viral immunohistochemical studies on CeMV infection among Western Mediterranean, Northeast-Central, and Southwestern Atlantic cetaceans. PloS One 14, 1–21.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Díaz-Delgado, J., Sierra, E., Vela, A. I., Arbelo, M., Zucca, D., Groch, K. R. and Fernández, A. (2017): Coinfection by Streptococcus phocae and cetacean morbillivirus in a short-beaked common dolphin Delphinus delphis. Dis. Aquat. Org. 124, 247–252.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Geraci, J. R. and Lounsbury, V. J. (2005): Marine Mammals Ashore: A Field Guide for Strandings. 2nd ed. National Aquarium in Baltimore, Baltimore, MD. 382 pp.

    • Search Google Scholar
    • Export Citation

  • Giorda, F., Ballardini, M., Di Guardo, G., Pintore, M. D., Grattarola, C., Iulini, B., Mignone, W., Goria, M., Serracca, L., Varello, K., Dondo, A., Acutis, P. L., Garibaldi, F., Scaglione, F. E., Gustinelli, A., Mazzariol, S., Di Francesco, C. E., Tittarelli, C., Casalone, C. and Pautasso, A. (2017): Postmortem findings in cetaceans found stranded in the Pelagos sanctuary, Italy, 2007–14. J. Wildl. Dis. 53, 795–803.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Pautasso, A., Iulini, B., Grattarola, C., Giorda, F., Goria, M., Peletto, S., Masoero, L., Mignone, W., Varello, K., Petrella, A., Carbone, A., Pintore, A., Denurra, D., Scholl, F., Cersini, A., Puleio, R., Purpari, G., Lucifora, G., Fusco, G., Di Guardo, G., Mazzariol, S. and Casalone C. (2019): Novel dolphin morbillivirus (DMV) outbreak among Mediterranean striped dolphins Stenella coeruleoalba in Italian waters. Dis. Aquat. Org. 132, 215–220.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Peletto, S., Caruso, C., Cerutti, F., Modesto, P., Biolatti, C. Pautasso, A., Grattarola, C., Giorda, F., Mazzariol, S., Mignone, W., Masoero, L., Casalone, C. and Acutis, P. L. (2018): Efficient isolation on Vero.DogSLAMtag cells and full genome characterization of Dolphin Morbillivirus (DMV) by next generation sequencing. Sci. Rep. 8, 1–8.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Sergeant, E. S. G. (2018): Epitools Epidemiological Calculators, Ausvet Pty Ltd. Available from: http://epitools.ausvet.com.au.

  • Smedley, R. C., Patterson, J. S., Miller, R., Massey, J. P., Wise, A. G., Maes, R. K., Wu, P., Kaneene, J. B. and Kiupel, M. (2007): Sensitivity and specificity of monoclonal and polyclonal immunohistochemical staining for West Nile virus in various organs from American crows (Corvus brachyrhynchos). BMC Infect. Dis. 7, 1–9.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Stanton, J. B., Brown, C. C., Poet, S., Lipscomb, T. P., Saliki, J. and Frasca, S., Jr. (2004): Retrospective differentiation of canine distemper virus and phocine distemper virus in phocids. J. Wildl. Dis. 40, 53–59.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Van Bressem, M. F., Duignan, P. J., Banyard, A., Barbieri, M., Colegrove, K. M., De Guise, S., Di Guardo, G., Dobson, A., Domingo, M., Fauquier, D., Fernandez, A., Goldstein, T., Grenfell, B., Groch, K. R., Gulland, F., Jensen, B. A., Jepson, P. D., Hall, A., Kuiken, T., Mazzariol, S., Morris, S. E., Nielsen, O., Raga, J. A., Rowles, T. K., Saliki, J., Sierra, E., Stephens, N., Stone, B., Tomo, I., Wang, J., Waltzek, T. and Wellehan, J. F. (2014): Cetacean morbillivirus: current knowledge and future directions. Viruses 6, 5145–5181.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Verna, F., Giorda, F., Miceli, I., Rizzo, G., Pautasso, A., Romano, A., Iulini, B., Pintore, M. D., Mignone, W., Grattarola, C., Bozzetta, E., Varello, K., Dondo, A., Casalone, C. and Goria, M. (2017): Detection of morbillivirus infection by RT-PCR RFLP analysis in cetaceans and carnivores. J. Virol. Methods 247, 22–27.

    • Crossref
    • Search Google Scholar
    • Export Citation

  • Yang, W. C., Pang, V. F., Jeng, C. R., Chou, L. S. and Chueh, L. L. (2006): Morbilliviral infection in a pygmy sperm whale (Kogia breviceps) from Taiwanese waters. Vet. Microbiol. 116, 69–76.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Collapse
  • Expand
Cover Acta Veterinaria Hungarica
Acta Veterinaria Hungarica
Print ISSN:
0236-6290
Online ISSN:
1588-2705

Senior editors

Editor-in-Chief: Ferenc BASKA

Editorial assistant: Szilvia PÁLINKÁS

 

Editorial Board

  • Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
  • Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • János GÁL (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 287 7073 (ed.-in-chief) or (36 1) 467 4081 (editor)

E-mail: acta.veterinaria@univet.hu (ed.-in-chief)

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture, Biology and Environmental Sciences
  • Elsevier Science Navigator
  • Focus On: Veterinary Science and Medicine
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

2023  
Web of Science  
Journal Impact Factor 0.7
Rank by Impact Factor Q3 (Veterinary Sciences)
Journal Citation Indicator 0.4
Scopus  
CiteScore 1.8
CiteScore rank Q2 (General Veterinary)
SNIP 0.39
Scimago  
SJR index 0.258
SJR Q rank Q3

Acta Veterinaria Hungarica
Publication Model Hybrid
Submission Fee none
Article Processing Charge 1100 EUR/article
Printed Color Illustrations 40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2025 Online subsscription: 832 EUR / 916 USD
Print + online subscription: 960 EUR / 1054 USD
Subscription Information Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation		 1951
Volumes
per Year		 1
Issues
per Year		 4
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2024 17 0 0
Jul 2024 23 0 0
Aug 2024 28 1 0
Sep 2024 43 0 0
Oct 2024 144 0 0
Nov 2024 43 1 1
Dec 2024 2 0 0