Acta Veterinaria Hungarica
Volume/Issue:
Volume 70: Issue 1
Serum protein electrophoretogram profile detected in apparently healthy cats infected with Leishmania infantum – Short communication
Authors:
Sergio Villanueva-Saz Animal Pathology Department, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain
Clinical Immunology Laboratory, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain

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https://orcid.org/0000-0001-6209-4282
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Jacobo Giner Animal Pathology Department, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain
Clinical Immunology Laboratory, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain

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Antonio Fernández Animal Pathology Department, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain
Clinical Immunology Laboratory, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain

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María Magdalena Alcover Department de Biologia, Salut i Medi Ambient, Facultat de Farmacia, Universitat de Barcelona, Av. de Joan XXIII 27-31, 08028, Barcelona, Spain

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Cristina Riera Department de Biologia, Salut i Medi Ambient, Facultat de Farmacia, Universitat de Barcelona, Av. de Joan XXIII 27-31, 08028, Barcelona, Spain

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Roser Fisa Department de Biologia, Salut i Medi Ambient, Facultat de Farmacia, Universitat de Barcelona, Av. de Joan XXIII 27-31, 08028, Barcelona, Spain

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Andrés Yzuel Clinical Immunology Laboratory, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain

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Ana González Animal Pathology Department, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain
Clinical Immunology Laboratory, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain

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Diana Marteles Clinical Immunology Laboratory, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain

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Maite Verde Animal Pathology Department, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain
Clinical Immunology Laboratory, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain

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Pages:
30–34
Online Publication Date:
28 Feb 2022
Publication Date:
23 Mar 2022
Article Category:
Brief Report
DOI:
https://doi.org/10.1556/004.2021.00055
Keywords:
agarose gel electrophoresis; cat; Leishmania infantum; PCR; serology
Restricted access

Abstract

The information about the clinical features of Leishmania infantum infection in cats is scarce. In this study, we evaluated the serum protein electrophoresis of samples from 19 infected but apparently healthy cats. To detect L. infantum infection, two serological tests, i.e. western blot (WB) and enzyme-linked immunosorbent assay (ELISA) as well as quantitative polymerase chain reaction (qPCR) on the blood samples were performed. Eventual infection by several selected bacterial and viral pathogens was also tested. All but one of the cats were found positive with WB. The WB-negative cat was positive by ELISA only. From the 18 WB-positive cats, only three were positive also by ELISA and eight with qPCR, including the only animal which was positive in all the three tests. No concomitant infections were detected in any of the cats. The main alteration of the proteinogram was characterised by an increase of the α-2 fraction. In the five cats with hypergammaglobulinaemia, the pattern detected was polyclonal. None of the cats were seropositive to any other pathogens tested. The presence of polyclonal gammopathy and elevation of the α-2 fraction could suggest the presence of active infection. In contrast, the only detection of an increase of the α-2 fraction alone with the presence of positive serological result could be associated by immune response activation against L. infantum.

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Cover Acta Veterinaria Hungarica
Acta Veterinaria Hungarica
Print ISSN:
0236-6290
Online ISSN:
1588-2705

Senior editors

Editor-in-Chief: Ferenc BASKA

Editorial assistant: Szilvia PÁLINKÁS

 

Editorial Board

  • Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
  • Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • János GÁL (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language English
Size A4
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Foundation		 1951
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per Year		 1
Issues
per Year		 4
Founder Magyar Tudományos Akadémia
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Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

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